Alteration of Bile Acid Transporter Expression in Patients with Early Cholestasis Following Living Donor Liver Transplantation

정은선,정은선,김병기,이연수,배시현,윤승규,최종영,박영민,김동구,김소윤 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.1

Background : Intrahepatic cholestasis can occur early after living donor liver transplantation (LDLT). We investigated the changes in the expressions of the bile acid transporters and the liver histology in the patients who suffered with early cholestasis (EC) following LDLT. Methods : The histological differences between 15 graft livers with EC after LDLT and 5 graft livers with biliary stricture following LDLT were evaluated. The hepatic mRNA levels of the bile canaliculi transporters (BSEP, MRP2, MRP3, MDR1, MDR3, NTCP) in 40 (20 graft livers, 20 matched donor livers) liver biopsy tissues were analyzed by performing real-time reverse-transcription polymerase chain reaction (RT-PCR). Results : Microscopic examination revealed hepatocellular and/or bile canalicular cholestasis around acinar zone 3 in the livers of the patients with EC. In the livers with biliary stricture, the cholestasis was dominantly observed in the hepatocytic cytoplasm and in the bile ductules around the portal area rather than around acinar zone 3. The BSEP and MRP2 mRNA levels in the EC livers were significantly reduced by 44% and 23%, respectively (p=0.000), compared to the matched donor livers. The levels of MDR3 and NTCP mRNA in the EC livers increased by 738% (p=0.000) and 281% (p<0.01), respectively. The change of the expressions of the bile acid transporters in the patients with biliary stricture was less significant than that in the EC group. Conclusions : These results suggest that the altered expressions of the bile acid transporters may play a role in the pathogenesis of EC following LDLT. Background : Intrahepatic cholestasis can occur early after living donor liver transplantation (LDLT). We investigated the changes in the expressions of the bile acid transporters and the liver histology in the patients who suffered with early cholestasis (EC) following LDLT. Methods : The histological differences between 15 graft livers with EC after LDLT and 5 graft livers with biliary stricture following LDLT were evaluated. The hepatic mRNA levels of the bile canaliculi transporters (BSEP, MRP2, MRP3, MDR1, MDR3, NTCP) in 40 (20 graft livers, 20 matched donor livers) liver biopsy tissues were analyzed by performing real-time reverse-transcription polymerase chain reaction (RT-PCR). Results : Microscopic examination revealed hepatocellular and/or bile canalicular cholestasis around acinar zone 3 in the livers of the patients with EC. In the livers with biliary stricture, the cholestasis was dominantly observed in the hepatocytic cytoplasm and in the bile ductules around the portal area rather than around acinar zone 3. The BSEP and MRP2 mRNA levels in the EC livers were significantly reduced by 44% and 23%, respectively (p=0.000), compared to the matched donor livers. The levels of MDR3 and NTCP mRNA in the EC livers increased by 738% (p=0.000) and 281% (p<0.01), respectively. The change of the expressions of the bile acid transporters in the patients with biliary stricture was less significant than that in the EC group. Conclusions : These results suggest that the altered expressions of the bile acid transporters may play a role in the pathogenesis of EC following LDLT.

The Role of Parental Support in Heritage Language Maintenance of Korean-Americans

정은선 한국외국어대학교 영미연구소 2019 영미연구 Vol.45 No.-

Among a number of factors that influence the maintenance or loss of a heritage language (HL), parental support has been found to be a critical predictor of HL maintenance. Despite the pivotal role played by parents in maintaining the HL, many immigrant parents face challenges in their involvement which is more complicated than it seems. By partaking in in-depth semi-structured interviews with second generation Korean-Americans, the present study examined how heritage speakers perceive parental involvement and whether they believe it plays a critical role in the successful maintenance of HL as previously thought. Three themes emerged from the interview data that represented common observable trends in the participants’ perceptions of and experience with the HL: (1) transmission of parental attitudes and beliefs was strong regardless of proficiency level and personal goals; (2) formal instruction and ethnic community played a limited role in maintaining and developing the HL; and (3) HL literacy practices in the home was of great importance in HL development and maintenance.

Mucinous Adenocarcinoma Involving the Ovary: Comparative Evaluation of the Classification Algorithms using Tumor Size and Laterality

정은선,배정훈,이아원,최영진,박종섭,이교영 대한의학회 2010 Journal of Korean medical science Vol.25 No.2

For intraoperative consultation of mucinous adenocarcinoma involving the ovary, it would be useful to have approaching methods in addition to the traditional limited microscopic findings in order to determine the nature of the tumors. Mucinous adenocarcinomas involving the ovaries were evaluated in 91 cases of metastatic mucinous adenocarcinomas and 19 cases of primary mucinous adenocarcinomas using both an original algorithm (unilateral ≥10 cm tumors were considered primary and unilateral <10 cm tumors or bilateral tumors were considered metastatic) and a modified cut-off size algorithm. With 10 cm, 13 cm, and 15 cm size cut-offs, the algorithm correctly classified primary and metastatic tumors in 82.7%, 87.3%, and 89.1% of cases and in 80.6%, 84.9%, and 87.1% of signet ring cell carcinoma (SRC) excluded cases. In total cases and SRC excluded cases, 98.0% and 97.2% of bilateral tumors were metastatic and 100% and 100% of unilateral tumors <10 cm were metastatic, respectively. In total cases and SRC excluded cases, 68.4% and 68.4%of unilateral tumors ≥15 cm were primary, respectively. The diagnostic algorithm using size and laterality, in addition to clinical history, preoperative image findings,and operative findings, is a useful adjunct tool for differentiation of metastatic mucinous adenocarcinomas from primary mucinous adenocarcinomas of the ovary.

장기간~Furosemide를 복용한 환자에서 신석회화를 동반한 급성 간질성 신염 1예

정은선,김용수,김영옥,천경아,윤선애,임국희,방병기,홍순화,박재명 대한신장학회 1998 Kidney Research and Clinical Practice Vol.17 No.6

Drug-induced acute interstitial nephritis is characterized by renal interstitial inflammatory cell infiltration and commonly presents as acute renal failure. This is caused mainly by methicillin, non-steroidal antiinflammatory drugs, sulfonamide diuretics such as thiazide, but cases induced by furosemide are rare. We report a patient with acute interstitial nephritis causing reversible acute renal failure and dermatitis while she was taking furosemide. A 37-year old woman was referred to our hospital because of generalized skin rash and non-oliguric acute renal failure. She had peripheral eosinophilia (1,577/mm3) and serum creatinine level of 6.8mg/dL. Skin biopsy showed leukoclastic vasculitis and percutaneous renal biopsy showed severe interstitial infiltration of lymph ocyte and mild interstitial fibrosis with focal tubular atrophy. After withdrawal of furosemide, renal function and skin lesions were completely recovered.

로맨틱 JEAN 패션디자인 연구

정은선,김정혜 한국니트디자인학회 2008 패션과 니트 Vol.6 No.1

Fashion is a cultural code most sensitively demonstrating movements in changing society; the jean fashion has grown not only in comfortable and functional everyday clothing but also in attractive high fashion; a decorative and feminine romantic style of the jean fashion has been remarkably developed in the 21st century. In designing the jean fashion by introducing aesthetic properties of the romantic fashion, this study developed and used various kinds of jean using flounce, ruffle, unraveling, taping, stitches, and cubic flower embroidery designs. Silhouettes and details were expressed in a modern and feminine image applying the line of corsets. On this basis, an attempt was made to present a method to expand expressions of the jean fashion and develop designs in pursuit of characterization and diversification. The purpose of this study is to develop materials to emphasize expressions of a new fashion and creators' individuality to expand the area of material expression and create styles for a new romantic jean fashion design. For this purpose, prior literature and researches were reviewed for the concept and features of the romantic fashion, properties of the romantic fashion, and the concept, history, kinds, and expressions of the jean fashion, along with collection of photo data from Internet websites and collection magazines to perform case analysis of the modern romantic fashion design and jean-using designs in the modern fashion. On this basis, eight suites of jean clothes were manufactured by introducing a romantic fashion. This study obtained the following conclusions. First, theoretical consideration of the romantic jean fashion demonstrated that it was development of materials and creation of creative designs which exerted great effects on designs and played a key role in the modern fashion. It is jean which is being used in various classes, regardless of age, gender, or rank, in the modern fashion using various materials; various detail expressions and feminine images of the romantic jean fashion have been developed markedly in this 21st century. The property of the modern romantic fashion is found to be sensuality caused by decorativeness and exposure, which functions in combination with various expressions of jean to make the romantic jean fashion remarkable today. Second, it was possible to develop handcraft-like, artistic jean for a material of a work and give feminine and aesthetic expression of the romantic jean fashion. Silk chiffon dyed in the same color with jean was mixed and matched to emphasize the soft and delicate style of the romantic fashion; it was possible to express an ample sense of quality by various expressions including cubic flower embroideries, stitches, taping, and unraveling. Third, four colors―light blue, indigo blue, grey, and black―were used to express the jean fashion with elegant romantic images. Accent was given by color arrangement with light colors of unraveled strands and stitches; it was possible to emphasize cuts and the lines of flower motives with accentual colors, such as white, yellow, and red. Fourth, it was possible to create a jean fashion in a specific style by introducing cuts of corsets and applying decorative details, such as flounce, frill, lace-up, and hook & eye, in order to emphasize feminine and decorative features of the romantic fashion in the style of works Fashion is a cultural code most sensitively demonstrating movements in changing society; the jean fashion has grown not only in comfortable and functional everyday clothing but also in attractive high fashion; a decorative and feminine romantic style of the jean fashion has been remarkably developed in the 21st century. In designing the jean fashion by introducing aesthetic properties of the romantic fashion, this study developed and used various kinds of jean using flounce, ruffle, unraveling, taping, stitches, and cubic flower embroidery designs. Silhouettes and details were expressed in a modern and feminine image applying the line of corsets. On this basis, an attempt was made to present a method to expand expressions of the jean fashion and develop designs in pursuit of characterization and diversification. The purpose of this study is to develop materials to emphasize expressions of a new fashion and creators' individuality to expand the area of material expression and create styles for a new romantic jean fashion design. For this purpose, prior literature and researches were reviewed for the concept and features of the romantic fashion, properties of the romantic fashion, and the concept, history, kinds, and expressions of the jean fashion, along with collection of photo data from Internet websites and collection magazines to perform case analysis of the modern romantic fashion design and jean-using designs in the modern fashion. On this basis, eight suites of jean clothes were manufactured by introducing a romantic fashion. This study obtained the following conclusions. First, theoretical consideration of the romantic jean fashion demonstrated that it was development of materials and creation of creative designs which exerted great effects on designs and played a key role in the modern fashion. It is jean which is being used in various classes, regardless of age, gender, or rank, in the modern fashion using various materials; various detail expressions and feminine images of the romantic jean fashion have been developed markedly in this 21st century. The property of the modern romantic fashion is found to be sensuality caused by decorativeness and exposure, which functions in combination with various expressions of jean to make the romantic jean fashion remarkable today. Second, it was possible to develop handcraft-like, artistic jean for a material of a work and give feminine and aesthetic expression of the romantic jean fashion. Silk chiffon dyed in the same color with jean was mixed and matched to emphasize the soft and delicate style of the romantic fashion; it was possible to express an ample sense of quality by various expressions including cubic flower embroideries, stitches, taping, and unraveling. Third, four colors―light blue, indigo blue, grey, and black―were used to express the jean fashion with elegant romantic images. Accent was given by color arrangement with light colors of unraveled strands and stitches; it was possible to emphasize cuts and the lines of flower motives with accentual colors, such as white, yellow, and red. Fourth, it was possible to create a jean fashion in a specific style by introducing cuts of corsets and applying decorative details, such as flounce, frill, lace-up, and hook & eye, in order to emphasize feminine and decorative features of the romantic fashion in the style of works

한반도 지역 미세먼지와 초미세먼지의 권역별 변화 경향 및 기상인자와의 관련성

정은선,이윤곤,이그림,박성빈,김광년 한국기상학회 2020 한국기상학회 학술대회 논문집 Vol.2020 No.10

육군 신병 교육 훈련 상황에서 심리적 강인성이 정신건강에 미치는 영향: 대처방식과 성취목표를 매개변인으로

정은선 한국상담심리학회 2007 한국심리학회지 상담 및 심리치료 Vol.19 No.1

This study examined the effect of psychological hardiness on mental health in the military basic training period using coping strategies and achievement goals as mediating variables. We administered measures of psychological hardiness, mental health, ways of coping, and achievement goals to 300 military recruits at the end of a demanding, 5-week basic training period. The results revealed that psychological hardiness, ways of coping and achievement goals were significantly correlated with mental health, as measured by psychological well-being and distress. The results of this study are important in revealing the effects of psychological health, coping, and achievement goals on the mental health of military recruits. In addition, this study is an important contribution because of its empirical focus on adjustment to military service. Implications for the future studies are suggested. 본 연구는 육군 현역훈련병을 대상으로 신병 교육 훈련 과정에서 심리적 강인성(이하 강인성), 대처방식, 성취목표가 정신건강에 어떠한 영향을 미치는지 알아보고자 하였다. 이를 위해 동일한 시기에 입소한 훈련병 300명을 대상으로 설문을 통해 강인성, 대처방식, 성취목표, 정신건강을 측정하고 그 결과를 분석하였다. 연구 결과, 강인성, 대처방식, 성취목표가 정신건강에 영향을 미치는 것으로 나타났다. 그 결과를 요약하면 다음과 같다. 첫째, 강인성이 높을수록(특히 몰입이 높을수록) 정신건강의 하위변인인 안녕감은 높고 불편감은 낮은 것으로 나타났다. 둘째, 문제대처가 높을수록 안녕감이 높고 불편감이 낮은 반면, 정서대처가 높을수록 안녕감이 낮고 불편감은 높은 것으로 나타났다. 셋째, 숙달목표를 가질수록 안녕감은 높고 불편감이 낮으며, 수행회피목표를 가질수록 안녕감은 낮고 불편감이 높은 것으로 나타났다. 마지막으로, 강인성이 정신건강에 영향을 미치는 과정을 대처방식과 성취목표가 매개하는 것으로 나타났다. 본 연구를 통해 강인성이 실제 스트레스 상황에서 정신건강에 긍정적인 영향을 미치고, 이 과정에서 대처양식, 성취목표의 매개적 역할에 대해 확인하였다. 신병 교육 훈련 기간 동안 훈련병의 심리적 특성을 경험적으로 밝히고 상담을 통한 개입의 필요성 등을 논의하였다.

난소에서 발생한 내배엽동 종양의 복수의 세포학적 소견 - 1례 보고 -

정은선,김영신,강창석,심상인,Jeong, Eun-Sun,Kim, Young-Shin,Kang, Chang-Suk,Shim, Sang-In 대한세포병리학회 1995 대한세포병리학회지 Vol.6 No.2

The cytologic findings of endodermal sinus tumor of the ovary are reported. The cytologic preperations were obtained from ascitic specimens. The findings on Papanicolaou-stained smears included a clean background and poorly preserved atypical cells loosely arranged in irregular or papillary groups. At high magnification, the cells had ill-defined and microvacuolated cytoplasms, with an increased nuclear-cytoplasmic ratio and prominent nucleoli. Hyaline globules characteristic of the alpha-fetoprotein (AFP)-synthesizing cells of endodermal sinus tumor were observed within the cells with periodic-acid-Schiff(PAS) stain. The presence of PAS-positive hyaline globules can be regarded, therefore, as a diagnostic clue to endodermal sinus tumor in an appropriate clinical setting and in the presence of AFP production. The cytologic findings in a cell block is important, in addition to smears, to obtain more diagnostic clues. A preliminary cytomorphologic diagnosis of this highly malignant tumor is valuable as a guide in planning further immunocytochemical and serologic studies.

고관절치환술 적정성 평가 방안 마련

정은선,유혜림,류미경 건강보험심사평가원 심사평가연구소 2019 연구보고서 Vol.2018 No.0

한국과 중국 지상미세먼지농도의 최근 변화 경향과 기상인자와의 관련성 분석

정은선,김광년,이윤곤 한국기상학회 2019 한국기상학회 학술대회 논문집 Vol.2019 No.10