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초임계 이산화탄소 및 유기용매를 이용하여 추출된붕장어(Conger myriaster) 오일의 품질특성
박진석,조연진,정유린,전병수 한국청정기술학회 2019 청정기술 Vol.25 No.4
In this study, the extraction of Conger myriaster oil by using supercritical carbon dioxide (SC-CO2) and organic solventwas investigated. The extraction conditions conducted for SC-CO2 varied for pressure (25, 30 MPa) and temperature (45, 55 ℃),while the SC-CO2 flow rate was kept constant during the experiment (27 g min-1) and hexane was used as a conventional organicsolvent. The extraction yield indicated that the best extraction condition would be SC-CO2 at 55 ℃ and 30 MPa, resulting in thehighest yield of 37.73 ± 0.14%. The oils were characterized for their fatty acid (FAs) composition using gas chromatography, whileit was revealed that the major FAs were mystric acid, palmitoleic acid, oleic acid, electroosapentaenoic acid (EPA), anddocosahexaenoic acid (DHA). The oxidation stability of the extracted C. myriaster oil was evaluated by measuring the acid value,peroxide value, and free fatty acid. The best oxidative stability was obtained from SC-CO2 extracted oil at 30 MPa and 55 ℃. There was a significant difference in the color properties of the SC-CO2 and hexane extracted oils, with the SC-CO2 extractedoil showing better chromaticity than the oil extracted using hexane. Extracting oils from C. myriaster with SC-CO2 could bringbetter economic benefits than using organic solvents. When supercritical carbon dioxide was used, there was no post-treatmentprocess; thus, it was confirmed that this is a more environmentally friendly oil extraction method. 본 연구는 초임계 이산화탄소 및 유기용매를 이용하여 동결건조된 붕장어로부터 초임계 이산화탄소 및 유기용매를 이용하여 오일을 추출하고 그 특성을 파악하였다. 초임계 이산화탄소의 경우 압력(25, 30 MPa) 및 온도(45, 55 ℃) 조건을 변화시켜실험을 수행하였으며, 초임계 이산화탄소의 유량(27 g min-1)은 실험 중 일정하게 유지되었다. 유기용매로는 헥산(hexane)을사용하였다. 오일 추출 수율의 경우 55 ℃, 30 MPa에서 추출한 오일이 37.73 ± 0.14%로 가장 높은 수율을 나타내었다. 추출된 오일의 지방산 조성은 가스 크로마토그래피를 이용하여 분석하였으며, mystric acid, palmitic acid, palmitoleic acid, oleicacid, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA)가 붕장어 오일의 주요 지방산으로 확인되었다. 산가, 과산화물가 및 유리지방산을 측정함으로써 추출된 붕장어 오일의 산화 안정성을 평가하였으며, 55 ℃, 30 MPa에서 추출한 오일로부터 최상의 산화 안정성을 확인하였다. 소비자들의 선호도에 직접적인 영향을 끼치는 색도의 경우는 초임계 이산화탄소를통해 추출된 오일이 유기용매를 이용하여 추출된 오일보다 뛰어난 색도를 나타냄을 확인할 수 있었다. 붕장어로부터 초임계 이산화탄소를 이용하여 오일을 추출하게 되면 유기용매를 사용한 방법보다 더 나은 경제적 이익을 가져올 수 있으며, 초임계 이산화탄소를 이용할 경우 후처리 공정이 없기 때문에 더 친환경적인 오일의 추출법임을 확인하였다.
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정연겸,박진석,정유린,전병수,박대찬,이헌숙,장미순,심길보 한국수산과학회 2020 한국수산과학회지 Vol.53 No.3
We investigated the effect of the modified starch [physical treatment, acetate (SA-1, SA-2, and SA-3), hydroxypropyl(HS), and acetylated distarch adipate] content on the physicochemical properties of fish cakes, called Eomuk in Korea,during storage at -20°C. When wheat flour (native starch, NS) was used, the moisture content decreased duringfrozen storage, resulting in a lower water-holding capacity and higher expressible drip. Compared with NS, addingmodified starch had a lower effect on the moisture content during frozen storage. The water-holding capacity andexpressible drip differed with the type of modified starch added. The expressible drip was lowest with HS; the SA-3and HS modified starch were whiter than NS. Therefore, HS and SA-1 are suitable additives to improve the qualityof frozen fish cakes.
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수산물의 콜레스테롤 분석을 위한 콜레스테롤 분석법 개선 및 검증
정소희 ( So Hee Jung ),정유린 ( Yu Rin Jeong ),신혜영 ( Hye Young Shin ),김연계 ( Yeon-kye Kim ),윤나영 ( Na-young Yoon ) 한국수산과학회 2022 한국수산과학회지 Vol.55 No.2
Cholesterol is an essential component for maintaining health; however, excessive consumption can lead to diseases. Thus, continuous monitoring of cholesterol content is important in food research. The cholesterol analysis method used in Korea follows the Korean Food Standards Codex. As this method uses gas chromatography, derivatization of the sample is required, and analysis time is more than 30 min. Kim developed a new method using liquid chromatog raphy; however saponification by the non-heat saponification method is insufficient. To address these limitations, a new cholesterol analysis method was developed and verified. The correlation coefficients for the cholesterol standard (STD) were maintained above 0.99. The limit of detection and limit of quantitation of cholesterol STD were 2.41 μg/mL and 7.31 μg/mL, respectively. The accuracies for cholesterol were 92.21-99.02%. The developed analytical method was also verified using three standard reference materials, and their accuracies were 93.71-97.09%. In addi tion, the cholesterol content of fishes was analyzed, and the values were compared with those recorded by the United States Department of Agriculture. Our results suggest that this method could be used as a new analytical method for cholesterol in seafood.
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강세원 ( Se-won Kang ),김은지 ( Eun Ji Kim ),정유린 ( Yu-rin Jung ),고해주 ( Hae Ju Ko ) 대한화장품학회 2018 대한화장품학회지 Vol.44 No.3
조류 소재에 관한 연구는 꾸준히 진행되고 있다. 본 연구에서는 미역, 다시마, 불등가사리 등 해조류를 혼합한 발효추출물의 항산화, 미백, 보습의 활성 실험을 진행하였다. 또한 유산균으로는 김치유산균 Lactobacillus sakei균주를 이용하였다. 복합해조류 발효추출물의 생리활성 실험을 진행하였으며, 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) 라디칼 소거능에서는 양성대조군 보다 높은 저해효능을 보였다. 미백효능 실험에서는 3,4-dihydroxy-L-phenylalanine (L-DOPA), mushroom tyrosinase 실험을 수행하였으며, 발효 전보다 발효 후의 미백활성이 높게 나타나는 것을 확인할 수 있었다. 한편, 해조류 소재의 유효성분인 fucose에 대한 HPLC분석을 수행하였으며, 용매조건에 대한 표준화 방법을 새로이 정립하였다. 이 연구는 복합해조류 추출물이 항산화, 미백 등 화장품 소재로서 가능성을 제안할 수 있다. Studies on seaweed-based materials have been progressing steadily day by day. In this experiment, we checked the anti-oxidant, whitening, and moisturizing activities of fermented extract from a mixture of Undariapinnatifida, Saccharina japonica, and Gloiopeltis furcate. Also, Lactobacillus sakei strains of kimchi were used as the lactic acid bacteria. The physiological status of the combined seaweed extracts was also investigated. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis-3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical scavenging results showed that the inhibitory effects of the combined seaweed extracts were higher than the positive control. Furthermore, 3,4-dihydroxy-L-phenylalanine (L-DOPA) and Mushroom tyrosinase tests were conducted during the whitening efficacy experiment. Hence, it was confirmed that the whitening activity of fermented extracts was greater than the extracts without fermentation. HPLC analysis of fucose (an active ingredient of seaweed) was also performed and a standard method for solvent conditions was newly established. This study suggests that the composite of algae extract has potentials to be used as anti-oxidant and whitening agents in cosmetics.
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