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인슐린 농도 변화에 따른 인슐린 내재화 ( Internalization ) 와 Degradation 의 변화
박원근(Won Kun Park),최웅환(Woong Hwan Choi),한인권(In Kwon Han),김선우(Sun Woo Kim),정운원(Woon Won Jung),문인걸(In Gul Moon) 대한내과학회 1988 대한내과학회지 Vol.34 No.4
N/A This study was conducted to investigate the effect of ambient concentration of insulin on insulin internalization and degradation in human erythrocytes. The absolute amount of internalized insulin was quantitated by multiplying the concentration of insulin by the percent specifically bound and internalized (by acid extraction method, PBS, pH 5.7) at the various insulin concentration (1.4, 2.5, 12.4, 112.4 ng/m1). The insulin degradation products (IDP) was assayed by using Sephadex G-50 column, HPLC with radioisotope detector in human erythrocytes which were incubated (37℃) at the various insulin concentration (1.43, 2.76, 14.76, 26.60ng/ml). Insulin internalization (ng/ml) increased in human erythrocytes with increasing concentration of insulin (3hr incubation time, 0.336 at 1.4, 0.275, at 2,5, 3.844 at 12.4, and 24.728ng/ml at 112.4ng/ml, 4hr incubation time, 0. 476, at 1.4, 0.925 at 2.5, 4.712 at 12.4 and 38.216ng/ml at 112.4ng/ml). Insulin degradation products (%) increased (2hr incubation time, 20.9 at 1,43; 38.0 at 2.76 and 38.0% at 14.76ng/ml) but no more than at a level of insulin concentration (2.76ng/ml) in normal human erythrocytes. In diabetic human erythrocytes, insulin degradation products (%) also increased (3br incubation time, 14.1 at 1.43, 19.0 at 2,76, 23.0 at 14.76, and 23.2% at 26.60ng/ml, 4hr incubation time, 39.4 at 1.43, 46.9 at ~2.76, 52. S at 14.76 and 53.0% at 26.60ng/ml) but no more than at a level of insulin concentration (14.76ng/ml), This result indicated that the ambient concentration of insulin may play an important role in the regulation of intracellular processing of insulin.
Type 2 DM 환자에서 적혈구를 이용한 인슐린 내재화율과 Degradation Products 에 관한 연구
최웅환(Woong Hwan Choi),박원근(Won Kun Park),한인권(In Kwon Han),김선우(Sun Woo Kim),정운원(Woon Won Jung),문인걸(In Gul Moon) 대한내과학회 1988 대한내과학회지 Vol.34 No.3
N/A We studied insulin internalization and degradation at 37℃ in human erythrocytes from patients with type II DM and normal subjects. The internalization of 125I-Insulin in human erythrocytes was studied by using an acid extraction technique (pH 5.7, PBS) from 54 patients with type II DM and 14 normal subjects. Insulin degradation products was assayed with using Sephadex G-50 column, HPLC with radioisotope detector from 4 patients with type II DM and 14 normal subjects. The maximal rate of insulin internalization (Incubation time: 4hours, at 37℃) was decreased in patients with type II DM [51.40±14.67% (±SD) VS 80.23±7.73 % (±SD). P<0.001]. The maximal degradation products of insulin (Incubation time: 4hours, at 37℃) was decreased in patients with type II DM [62.50±10.50% (±SD) VS 87.75±6.66% (SD). p<0.001]. In conclusion, insulin internalization and degradation in human eryhrocytes from patients with type II DM are significantly reduced. These defect may be related to the cellular insulin resistance present in these patients.