Generation of Demyelination Models by Targeted Ablation of Oligodendrocytes in the Zebrafish CNS

정아영,김판수,김수현,김은미,김도현,정인영,김환기,류재호,김철희,서진호,박해철,최준 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.1

Demyelination is the pathological process by which myelin sheaths are lost from around axons, and is usually caused by a direct insult targeted at the oligodendrocytes in the vertebrate central nervous system (CNS). A demyelinated CNS is usually remyelinated by a population of oligodendrocyte progenitor cells, which are widely distributed throughout the adult CNS. However, myelin disruption and remyelination failure affect the normal function of the nervous system, causing human diseases such as multiple sclerosis. In spite of numerous studies aimed at understanding the remyelination process, many questions still remain unanswered. Therefore, to study remyelination mechanisms in vivo, a demyelination animal model was generated using a transgenic zebrafish system in which oligodendrocytes are conditionally ablated in the larval and adult CNS. In this transgenic system, bacterial nitroreductase enzyme (NTR), which converts the prodrug metronidazole (Mtz) into a cytotoxic DNA cross-linking agent, is expressed in oligodendrocyte lineage cells under the control of the mbp and sox10 promoter. Exposure of transgenic zebrafish to Mtz-containing media resulted in rapid ablation of oligodendrocytes and CNS demyelination within 48 h, but removal of Mtz medium led to efficient remyelination of the demyelinated CNS within 7 days. In addition, the demyelination and remyelination processes could be easily observed in living transgenic zebrafish by detecting the fluorescent protein, mCherry, indicating that this transgenic system can be used as a valuable animal model to study the remyelination process in vivo, and to conduct high-throughput primary screens for new drugs that facilitate remyelination.

사회부과 완벽주의와 학업스트레스가 시험불안에 미치는 영향: 부적응적 인지적 정서조절전략의 조절된 매개효과

정아영,김은하,신희천 한국심리학회산하학교심리학회 2017 한국심리학회지 학교 Vol.14 No.3

The purpose of this study was to examine the moderated mediating effect of maladaptive cognitive emotion regulation strategies through academic stress on the relationship between socially-prescribed perfectionism and test anxiety. A total of 604 Korean high school students, including 367 males and 237 females, participated in this study by responding to the following questionnaires: Multidimensional Perfectionism Scale (HMPS), Academic Stress Scale, Korean version of Cognitive Emotion Regulation Questionnaire (K-CERQ), Test Anxiety Inventory-Korea (TAI-K). The collected data were analyzed using SPSS Macro. The main findings were as follows. First, using three-step mediated regression analysis, the significant partial mediating effect of academic stress on the relationship between socially-prescribed perfectionism and test anxiety was found. Second, using hierarchial multiple regression analysis, it was found that maladaptive cognitive emotion regulation strategies moderatedthe relationship between academic stress and test anxiety. Third, using SPSS Macro, the moderated mediating effect of maladaptive cognitive emotion regulation strategies (especially, rumination, self-blame, and catastrophizing) on the relationship between socially-prescribed perfectionism, academic stress, and test anxiety was found. Based on the result of this study, we discussed several implications for counseling and education, limitations of the study, and suggestions for future research. 본 연구에서는 인문계 고등학생을 대상으로 사회부과 완벽주의와 시험불안 간의 관계에서 학업스트레스가 매개하는지 살펴보고, 부적응적 인지적 정서조절전략이 이러한 매개효과를 조절하는지 살펴보았다. 이를 위해 인문계 고등학교에 재학 중인 604명의 고등학생을 대상으로 사회부과 완벽주의, 학업스트레스, 부적응적 인지적 정서조절전략, 시험불안을 측정하였고, SPSS Macro를 사용하여 자료를 분석하였다. 본 연구의 결과는 다음과 같다. 첫째, 사회부과 완벽주의와 시험불안 간의 관계를 학업스트레스가 부분매개 하는 것으로 나타났다. 둘째, 부적응적 인지적 정서조절전략이 학업스트레스와 시험불안 간의 관계를 조절하는 것으로 나타났는데, 부적응적 인지적 정서조절전략을 많이 사용할수록 학업스트레스가 시험불안에 미치는 부정적인 영향이 커지는 것으로 확인되었다. 셋째, 조절된 매개효과가 나타났는데, 사회부과 완벽주의와 시험불안 간의 관계에서 학업스트레스의 매개효과는 부적응적 인지적 정서조절전략(특히, 반추, 자기비난, 파국화)을 많이 사용할수록 높아지는 것으로 나타났다. 이러한 결과를 바탕으로 본 연구의 의의, 후속 연구를 위한 제언, 그리고 상담적 함의를 논의하였다.

공연예술연습공간으로 리모델링된 교육시설의 음향특성

정아영,김재수 한국문화공간건축학회 2015 한국문화공간건축학회논문집 Vol.- No.51

Aged public idle facilities or school facilities left empty in urban areas have recently caused social problems. For this reason, each local government plans to remodel such facilities into performing arts practice spaces with the objective of developing local culture and arts and forming cultural communities. From this perspective, this study aimed to assess the changes of spatial elements and finishing materials and improvement in acoustic performance after remodeling in former C Girls' High School recently remodeled into a performing arts practice space. Reverberation time before remodeling ranged from 1.22 to 2.87 seconds, causing acoustic defects due to excessive reverberation. As spatial elements and finishing materials were changed at the stage of remodeling to improve sound-absorbing power and room constant, reverberation time was significantly improved after remodeling: 0.51∼1.00 seconds. In addition, such acoustic performance parameters as the sound pressure level, vocal definition, musical definition, and the vocal delivery index were at more satisfactory levels after remodeling. On the basis of these results, remodeling of neglected educational facilities into performing arts practice spaces could provide necessary spaces for residents and cultural groups to go over their lines and practice movements for plays or dances.

개에서 Malassezia 피부염에 대한 itraconazole 치료 18례

정아영,허우필,정효훈,엄기동,이근우,오태호 한국임상수의학회 2005 한국임상수의학회지 Vol.22 No.2

Itraconazole was found to be an effective antifungal for the treatment of canine Malassezia dermatitis (MD). MD was diagnosed in 18 dogs, which were treated with itraconazole administered orally at 5mg/kg of body weight, q12hrs, for 21 to 30 days. High prevalence breeds of MD were Maltese (22%), Cocker Spaniel (17%), Pekingese (11%), and Vizsla (11%). The dermatological signs of Malassezia dermatitis were crust (31%), alopecia (25%), hyperpigmentation (25%), scales (19%), erythema (13%), lichenification (11%), pustule (11%), ear swelling (11%), papules (5%), and offensive odor (5%). Commonly affected areas were ear canal (41%), axillae (18%), groin (15%), perianal (12%), ventral aspect of the neck (9%), interdigital spaces (1%), and muzzle (1%). Sixty seven percent of dogs with MD had cocci. Clinical responses of itraconazole were seen good, moderate, no responses of itraconazole, in 89%, 0%, and 11%, respectively, according to the owner's satisfaction to follow up call. Recurrence was detected on five good responsive dogs and adverse effects of the treatment were detected in only one dog. On the basis of this clinical study, itraconazole is a good choice in the treatment of canine Malassezia dermatitis. Efficacy, frequency of administration and veterinary approval are the major advantages.

일본 공립도서관의 유형별 특성 분석 - 서비스내용과 서비스공간의 연계적 고찰을 중심으로 -

정아영,Jung, A-Young 한국비블리아학회 2012 한국비블리아학회지 Vol.23 No.2

본 연구는 도서관 이용자의 요구, 이용목적 등이 점점 다양화되고 있는 현시점에서 도서관이 제공하고 있는 서비스내용과 서비스공간의 연계적인 고찰의 필요성에 주목하고 있다. 본 연구에서는 일본 공립도서관을 대상으로 서비스내용과 서비스공간의 연계성을 중심으로, 공립도서관의 유형분류를 실시하였으며, 이를 바탕으로 일본 공립도서관의 유형특성을 분석하였다. 본 연구는 향후 한국 공립도서관의 서비스내용과 연계성 있는 서비스공간계획의 실현에 도움이 될 기초적인 데이터를 마련하는 데 궁극적인 목적이 있다. 조사는 일본 공립도서관을 대상으로 제공되고 있는 서비스의 내용과 서비스제공에 사용되고 있는 서비스공간에 관한 실태를 조사 하였으며, 조사된 데이터는 수량화III류 분석, 군집분석 등 다차원데이터분석법을 사용하여 분석하였다. 도서관의 특성분석의 관점 추출을 진행한 결과, "제공하고 있는 정보의 분야, 정보제공의 방법, 서비스의 제공대상"의 관점으로 추출 할 수 있었다. 또한 44개 도서관의 유형은 (1) 집단을 대상으로 취업, 직업 분야의 정보를 제공하는 도서관, (2) 취업, 직업 정보를 대응서비스지원공간을 통해 제공하고 있는 도서관, (3) 개인을 대상으로 교육 분야의 정보를 제공하고 있는 도서관, (4) 집단을 대상으로 교육 분야의 정보를 제공하고 있는 도서관, (5) 대응서비스공간을 중심으로 정보를 제공 하고 있는 도서관, (6) 개인을 대상으로 하는 서비스제공 중심의 도서관의 6가지로 특성 분류되었다. This study takes note on the need for the consideration of the relationship between the service contents and service space which libraries provide, since at the moment the demands of library users, purpose of using it, etc. gradually become varied. In this study, type classification of public libraries was done focused on the relationship between service contents and service space, targeting Japanese public libraries. Through linking approach between service contents and service space, the analysis of Japanese public libraries' characteristics and type characteristics could be captured. This study has ultimately aimed to prepare for the basic data which will help Korean public libraries to make connection between service contents and service space in the future. The collected service contents and service space had been analyzed by multi-dimensional data analysis methods such as the "Quantification Theory Analysis Type III" and "Cluster Analysis". After characters of libraries were analyzed, the findings of this study show the views such as "the field of information provided, the method of information provision, and the object of service provision." And types of 44 libraries are classified into those which have 6 kinds of characteristics as follows : (1) group-oriented libraries which provide the jobs and employment information, (2) libraries which provide the jobs and employment information through the counselling space, (3) individual-oriented libraries which provide the information of education-related fields, (4) group-oriented libraries which provide the information of education-related fields, (5) libraries which provide information focusing on the counselling space, (6) individual-oriented libraries which focused on the service provision.

Factors That Affect the Rehabilitation Duration in Patients With Congenital Muscular Torticollis

정아영,강은영,이성훈,남두현,전지환,김효정 대한재활의학회 2015 Annals of Rehabilitation Medicine Vol.39 No.1

Objective To determine which factors affect the rehabilitation duration in patients with congenital muscular torticollis (CMT) and to predict the duration of rehabilitation and prognosis.Methods One hundred and eighteen patients (79 males and 39 females) who were diagnosed with CMT and received physical therapy were enrolled in this study. We retrospectively reviewed the information in terms of sex, gestational age, birth weight, methods of delivery, fetal presentation, age at diagnosis, the affected sternocleidomastoid (SCM) muscle site, SCM muscle thickness, ratio of muscle thickness on the affected side to that on the unaffected side (called the ‘abnormal/normal [A/N] ratio’), and range of motion for cervical rotation and side bending.Results The SCM muscle thickness and A/N ratio had a positive linear relationship with the rehabilitation duration. Patients who were in the breech position needed longer rehabilitation. The birth weight and age at diagnosis were negatively correlated with the rehabilitation duration. However, the cervical range of motion, mass site, sex, gestational age, and methods of delivery were not correlated with the rehabilitation duration.Conclusion Patients with a thicker SCM, lower birth weight, and history of breech delivery had a longer rehabilitation duration.

Biological effects of microplastics in Caenorhabditis elegans

정아영,김경원 환경독성보건학회 2022 한국독성학회 심포지움 및 학술발표회 Vol.2022 No.5

The Analysis on the Emotional Evaluation Methods of Environmental Design Elements Using the Eye Mark Recorder and the Repertory Grid Method

정아영 한국콘텐츠학회 2011 International Journal of Contents Vol.7 No.4

This paper is an inquiry into the human sensibility on view from high-rise housing in a viewpoint of architectural environment design that pay attention to multi-family high-rise housing which is becomes a trend in recent years. And this paper is the fundamental approach for applying this result to later architectural environmental plan of multi-family high-rise housing. In this paper, we used repertory grid method as a cognitive psychological way of analysis and eye mark recorder measurement experiments as a physiological way of examination. In the conclusions, examined human sensibilities on views-focused on the analysis result of eye mark recorder measurement experiments through repertory grid method that are carried on the main subject-and described problems after this and possibilities in the same time.

일본 공립도서관의 현황에 관한 조사연구

정아영 한국청소년시설환경학회 2014 한국청소년시설환경학회 학술대회 Vol.2014 No.1

국내 공연장의 프로세니움 아치 분석

정아영,김재수 한국문화공간건축학회 2021 한국문화공간건축학회논문집 Vol.- No.75

Most of the Hall built in the country have a proscenium arch that separates the audience from the stage. The Proscenium Arch has a visual effect, with the eyes facing the stage such as a picture frame, which is a necessary consideration when designing the Performance Halls. However, the lack of standards or statistical data for arch shape in the proper proscenium, It is having difficulty designing and using. From this point of view, In this study, we compared and analyzed the characteristics and specifications of the proscenium arch for each of the 106 Performance Halls built in Korea. As a result of analysis of the specifications of the Performance Halls, in the case of the Korean Traditional music hall, height 7 m, width 14.3 m, aspect ratio 1: 2.06, arch area 101.7 m2, the theater is 5.1 m in height, 11.3 m in width, aspect ratio 1: The arch area was 2.29, the arch area was 61.9 m2, the height of the multipurpose hall was 8.2 m, the width was 15.1 m, the aspect ratio was 1: 1.91, and the arch area was 128.9 m2. In addition, comparing the average size of the proscenium arches at each Performance Halls, it was in the order of  Multipurpose Hall> Korean Traditional music Hall> theater, and the aspect ratio of the proscenium Arch was shown in order of theater> Korean Traditional music Hall> Multipurpose Hall. In addition, as a result of analyzing the correlation between the width and height of the proscenium arch, the explanatory power (R2) of the Korean Traditional music Hall, theater, and multipurpose hall was 35.5%, 65.4%, and 55.2% respectively, and the arch area was 79.7%, 69.0%, and 64.6%. Therefore, knowing the number of seats will allow us somewhat to predict the size of the Proscenium arch. In addition, when comparing the size of the Korean proscenium arches to the standard of Western performances, the Korean Traditional Music Halls are the same as large theaters, medium theaters, and multipurpose halls are the size of opera houses and small theaters. Therefore, it is considered that these results can be used as basic data for determining the appropriate size of the proscenium arch during the design and construction of the Performance Halls.