Cumulus Free 생쥐 성숙란의 초자화 동결-융해 후 Simple Media에서의 수정 및 배 발달

정수경,김성건,이정재,오지현,이용호,김선행,Jung, Soo-Kyung,Kim, Sung-Kun,Lee, Jung-Jae,Oh, Ji-Hyun,Lee, Yong-Ho,Kim, Sun-Haeng 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.3

Objective: To observe the capability of fertilization and embryo development including blastocyst formation of the oocytes in simple media after thawing of the cryopreserved cumulus-free mouse oocytes by vitrification method. Methods: Oocytes were collected from 5 to 6 weeks old ICR female mice, and were denuded from the cumulus cells by 0.1% hyaluronidase. Recovered mature oocytes in study group were cryopreserved by vitrification method using EM grid for $5{\sim}7$ days. In brief, oocytes were exposed in dPBS containing 1.5 M EG and 5.5 M EG+1 M sucrose for 2.5 minutes and 20 seconds each, and then executed vitrification by plunging in LN2 after loading on EM grid. Thawing treated by exposure of 1, 0.5, 0.25 and 0.125 M sucrose solution for 2.5 minutes each in order and used for experiments. Spermatozoa aspirated form the epididymis of 12 weeks old ICR male mice were used for insemination after capacitation. T6 media containing 0.4% BSA were used for fertilization and development. Results: Survival and fertilization rates after thawing were 76.9% and 79.6% respectively. Fertilization rate was lower (p<0.005) than that of control group (92.9%). There was no difference in embryo developmental rates from 2-cell to morula, however, the blastocyst formation rate and mean cell numbers of blastocysts in study group (63.3%, $58.9{\pm}9.2$) were lower compared with those of control group (76.1%, $63.5{\pm}8.9$). Conclusion: Vitrification is an effective method for mouse mature oocyte cryopreservation with high survival and fertilization rate after thawing. And in simple media, fertilization rates and embryo development of frozen-thawed mouse oocytes are satisfactory.

지역사회복지관 가족복지서비스 분류체계에 관한 연구

정수경 ( Soo Kyung Jung ) 한국가족사회복지학회 2008 한국가족복지학 Vol.24 No.-

본 연구는 지역사회복지관 가족복지서비스 분류체계와 그 적용 현황을 분석해 봄으로써, 현 분류체계의 유용성을 파악하고 문제점을 찾아 새로운 분류체계 개발을 위한 논의를 하고자 한다. 문헌고찰을 하여 유용한 분류체계의 조건들을 탐색하고, 그 조건들을 중심으로 보건복지부의 분류체계를 분석해 본 후, 복지관의 분류체계 적용 현황을 조사하였다. 분석결과, 현 분류체계는 기준범주의 부재, 유형에 대한 상세설명의 불충분성, 중복가능성 그리고 포괄성을 담보할 수 없는 점, 실용성의 한계가 있는 것으로 분석되었다. 복지관에서의 적용 현황을 조사한 결과 분류체계를 적용하는 정도가 저조하였고, 적용하는 경우에도 지침에 부합되게 적용하지 못하는 경우가 많았으며, 사업을 구성하는 유용한 준거틀로 기능하지 못하는 문제점들이 발견되었다. 이를 통해 가족복지서비스를 위한 새로운 분류체계 개발 과제를 논의하였다. 본 연구는 가족복지서비스의 분류체계를 재정비함으로써, 복지관에서의 가족복지서비스를 새로운 틀에서 구조화하고 계획하고 평가하게 하여, 복지관에서의 가족복지서비스가 보다 전문적이고 체계적으로 정립되도록 기여할 수 있다는 데 의의가 있다. The purpose of this study is to analyze the usefulness of a typology used for family services in community welfare centers. This article has a brief review of the requirements necessary for a typology to be useful. Then, there is a critical review of the typology currently used to classify family services according to requirements. The final conclusion is that the present typology shows an absence of clear criteria, lack of detailed descriptions and clarity, and the possibility of overlap. It also does not cover all the family services in community welfare centers. Analyzing 292 community welfare centers on the basis of the Korean typology, it was found that the present typology does not work as a framework to organize family services. Only 24 percent of community welfare centers use the Korean typology as a classification system and its fidelity rate is low. Discussion on critical issues in developing a new typology followed. These findings contribute to promoting the development of a useful typology by researchers and policy makers that is responsive to changing contexts and to helping social workers effectively organize, plan, and evaluate all family services provided in community welfare centers.

산화티타늄을 이용한 VOCs의 광촉매 반응

정수경 ( Soo Kyung Jung ) 한국환경과학회 2008 한국환경과학회지 Vol.17 No.2

사회복지관 이혼가족지원사업에 관한 실태조사

정수경 ( Soo Kyung Jung ) 전주대학교 사회과학연구원 ( 구 사회과학종합연구소 ) 2008 社會科學論叢 Vol.24 No.1

VOCs가 흡착된 흡착제의 SFE 공정을 이용한 재생특성

정수경(Soo-Kyung Jung) 한국환경관리학회 2002 環境管理學會誌 Vol.8 No.4

VOCs의 광촉매용 산화티타늄 제조

정수경(Soo-Kyung Jung),이재동(Jae-Dong Lee) 한국환경관리학회 2007 環境管理學會誌 Vol.13 No.1

TiO₂에이로젤은 솔-젤공정과 높은 비표면적 요구로 습윤젤의 모세관 압력을 피하기 위해 초임계 건조공정에 의해 제조하였다. TiO₂에어로젤은 최소한의 구조변형을 위해 이산화탄소로 용매치환을 하며, 60℃, 3500 psi의 조건 하에서 실험을 수행하였으며, 에어로젤의 특성은 epoxide/TiCl₄의 비율과 수분의 양을 변화시키면서 측정하였다. 제조된 에어로젤의 광촉매 산화실험은 Benzene, toluene, m-xylene을 이용하여 실험하였으며, 상용되는 에이로젤과 그 특성을 비교하였다. 전구체로 propylene oxide를 사용할 경우에 비해 1,2-epoxy-butane를 사용할 경우 에어로젤의 비표면적, 기공부피, 기공크기가 현저히 감소하였다. 또한 6 mol의 epoxide를 사용할 경우 표면특성은 향상됨을 알 수 있었다. Titania gel formations were prepared by sol-gel method using titanium(N) chloride (TiCl4), and its characteristics were analyzed by varying the epoxide/TrCl₄ ratio and the amount of water. In the end, titania (TiO2) aerogel were prepared using supercritical drying process. VOCs such as benzene, toluene, and m-xylene (BTX) were oxidized using prepared titania aerogel and commercially available TiO2, and its performance was compared. The surface area, pore volume, and average pore diameter of 1,2-epoxybutane are significantly smaller than the propylene oxide. And the titania aerogels with 6 mol of epoxides have high surface areas, pore volumes, and average pore diameters.

Lipopolysaccharide로 자극한 RAW 264.7 세포에서 성체줄기세포 유래 엑소좀(exosome)의 면역 조절 효과

정수경 ( Soo Kyung Jung ),박미정 ( Mi Jeong Park ),이지현 ( Jienny Lee ),변정수 ( Jeong Su Byeon ),구나연 ( Na Yeon Gu ),조인수 ( In Soo Cho ),차상호 ( Sang Ho Cha ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.3

본 연구는 대식세포에서 LPS를 이용하여 염증 유사 세포모델을 만들고 염증 유사 대식세포 모델에서 성체줄기세포의 면역 조절 능력을 평가하였다. LPS 자극에 의해 증가된IL-1β, TNF-α 및 IL-10의 생성은 성체줄기세포를 공배양한 실험군 뿐만 아니라 성체줄기세포를 배양한 상층 배양액을 처리한 실험군에서도 동일한 효과를 나타내었으며, 또한 성체줄기세포 유래 엑소좀을 염증 유사 대식세포 모델에 처리하여 유사한 결과를 관찰하였다. 이 결과는 성체줄기세포 자체의 염증 억제 기능보다는 성체줄기세포 유래 엑소좀을 포함하여 성체줄기세포가 분비하는 bioactive molecules에 의해 세포 간 신호 전달이 이루어지고 있음을 의미하며, 이러한 엑소좀은 염증 관련 질환 분야에 치료적 적용이 가능하고 또한 새로운 염증 치료제 개발의 툴로 사용될 수 있음을 시사한다. Mesenchymal stem cells (MSCs) are multipotent stem cells that can be differentiated into a variety of cell types, including adipocytes, osteoblasts, chondrocytes, β-pancreatic islet cells, and neuronal cells. MSCs have been reported to exhibit immunomodulatory effects in many diseases. Many studies have reported that MSCs have distinct roles in modulating inflammatory and immune responses by releasing bioactive molecules. Exosomes are cell-derived vesicles present in biological fluids, including the blood, urine, and cultured medium of cell cultures. In this study, we investigated the immunomodulatory effects of mouse adipose tissue-derived MSCs (mAD-MSCs), cultured medium (MSC-CM) of mAD-MSCs, and mAD-MSCderived exosomes (MSC-Exo) on lipopolysaccharide (LPS)-induced RAW 264.7 cells. We observed that the expression levels of IL-1β, TNF-α, and IL-10 were significantly increased in LPS-stimulated RAW 264.7 cells compared to those in LPS-unstimulated RAW 264.7 cells. Additionally, these values were significantly (p < 0.05) decreased in mAD-MSCs-RAW 264.7 cell co-culture groups, MSC-CM-treated groups, and MSC-Exotreated groups. MSCs can modulate the immune system in part by secreting cytokines and growth factors. We observed that immunomodulatory factors such as IL-1β, TNF-α, and IL-10 were secreted by mAD-MSCs under co-culturing conditions of mAD-MSCs with activated RAW 264.7 cells. In addition, mAD MSC-derived exosomes exhibited similar immunomodulatory effects in activated RAW 264.7 cells. Therefore, our results suggest that mAD-MSCs have an immunomodulatory function through indirect contact.

학교사례관리 준거틀에 대한 사회복지사들의 인식

정수경(Jung Soo-kyung) 경성대학교 사회과학연구소 2009 社會科學硏究 Vol.25 No.4

본 연구는 전주지역의 교육복지투자지역지원사업 학교에서 지역사회교육전문가로 일하고 있는 사회복지사들이 효과적인 학교사례관리의 준거틀을 어떻게 인식하고 있는지를 탐색하여, 그로부터 지역에 적합한 학교사례관리 모델의 개발에 필요한 기초자료를 얻는데 연구의 목적이 있다. 이를 위해 2009년 4월 13일과 5월 17일에 9명의 사회복지사를 대상으로 포커스 그룹 인터뷰를 실시했다. 사회복지사의 인식을 밝혀내고 그 결과를 문헌의 준거틀과 비교분석한 결과 이론적 준거틀과 유사성 및 차이점을 보였다. 개별화, 생태학적 사정, 학생의 기능향상, 사례관리체계의 중요성, 연계 및 서비스 조정 역할, 직접 서비스 제공, 총괄평가에 있어서는 이론적 준거틀과 유사성을 보였다. 그러나 교사의 협력에 대한 과도한 중요성 인식, 욕구 사정 및 계획에서 학생 욕구에 대한 상대적으로 낮은 비중, 사례관리체계 구축의 실행가능성에 대한 낮은 기대, 구체적인 계획의 필요성에 대한 양가감정, 직접적인 학습지도의 중요성에 대한 인식, 형성평가에 대한 낮은 관심 등은 문헌의 준거틀과는 다른 것이었다. 이러한 연구결과를 토대로 학교사례관리의 원리와 목표, 과정에 대한 표준 및 실천 지침이 개발되어야 하고, 지역 교육청 차원에서의 학교사례관리체계 구축을 위한 노력이 필요하다는 제언을 하였다. The purpose of this study is to get insights from social workers for the development of the effective school case management in local Education Welfare Investment Priority Zone Project. For this, 2 focus group interviews were conducted in which 9 experienced social workers participated, on April 13th and May 17th. 2009. Comparing participants' perception to theoretical framework, some similarities and differences were found. Similarities were in individualization, ecological assessment, students' problem -focused goal, importance of creating supportive system, coordinating role, direct service, summative evaluation. Differently to the theoretical framework, participants showed over-focus on the cooperation with teachers, relatively low importance to students' needs, low expectation of the possibility on creating supportive system, ambivalence to the necessity of planning concretely, importance on the direct assistant for study and little concern in the formative evaluation. Based on these results, the development of standards and practice guidelines for the school case management, in principles, goals and process and the efforts toward creating support system for the school case management were suggested.

임상병리과의 활동기준원가 관리 적용에 관한 연구

정수경(Soo Kyung Jung),정기선(Key Sun Jung),최황규(Hwang Gue Choi),류규수(Kyu Soo Rhyu) 한국병원경영학회 2000 병원경영학회지 Vol.5 No.1

This empirical study, activity-based costing, a newly inroduced approach that gas proved to be an improvement over the conventional costing system in product or service costing, is applied at department of clinical pathology in K university hospital. The study subjects were 233 test procedures done in clinical laboratory of K university hospital. Activity analysis was done by interview, questionnaires, and time study, and the amount of resources consumed by each activity and their costs are then traced and applied to the laboratory tests. The main purpose of this study were to compare the test costs of activity-based costing with those of conventional costing, and test fees of medical insurance, and to provide accurate cost informations for the decision makers of hospital. The major findings of this study were as belows. 1. The cost drivers for application of activity-based costing at clinical laboratory were cases of sample collection, case of specimen, cases of test, and volume-related allocation bases such as direct labor hours and total revenue of each test. 2. The profits of each clinical laboratory fields analyzed by conventional costing were different from the profits analyzed by activity-based costing, especially in the field of Urinalysis(approximately over estimated 750%). 3. The standard full costs by conventional costing were quite different from the costs computed by using activity-based costing, and the difference is most significant with the tests of long labor time. 4. From the comparison between costs computed by using activity-based costing and medical insurance fees, some test fees were significantly lower than the costs, especially in the non-automated fields. As described in this study, activity-based costing provides more accurate cost information than does conventional costing system. The former approach is especially important in the health care industry including hospitals in which planning and controlling the costs services provided are the key to maintaining a healthy financial status for the organization. Despite the contribution of activity-based costing the economic as well as technical feasibilities of implementing such a cost accounting system in an organization must be evaluated. In the development of activity-based costing systems, an activity analysis has to be conducted to identify activities that consume resources. This involves a detailed study of the organization`s logistics and accounting information systems, and it is an expensive project in itself. Besides, it can be quite difficult and time consuming to identify and trace resource consumption to a specific activity. Thus the activity-based costing system should be implemented only when the decrease in cost of error far exceeds the increase in cost of measurement. By combining activity-based costing with standard costing, health are administrators can better plan and control the costs of health services provided while ensuring that the organization`s bottom line is healthy.

Cryptococus neoformans 감염조직의 병리조직학 및 초미세구조학적 연구

정수경(Soo-Kyung Jung),윤철종(Chul-Jong Yoon) 고려대학교 보건과학연구소 1999 보건과학논집 Vol.8 No.1