Deposition of Mucin Coat on Rabbit Embryos Cultured In Vitro Following Oviductal Transfer

Joung, S.Y.,Yang, J.H.,Im, K.S.,Lee, S.H.,Park, C.S.,Jin, D.I. 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8

Mucin coat is deposited on the embryos during passage through the oviduct in rabbit. When in vitro cultured blastocysts were transferred to the recipients, the lack of mucin coat might account in part for failure of pregnancy after transfer. The present study were carried out to investigate whether deposition of mucin coat were induced when in vitro cultured blastocysts were transferred to recipients. At 19-20 hours post coitus one cell embryos were collected by flushing oviducts. These embryos cultured for 72 hours were reached to blastocyst stage. And these blastocysts were transferred to the oviduct of asynchronized (one day later than the donors) and synchronized recipient. To confirm deposition of the mucin coat blastocysts transferred to the oviduct were recovered at 24 and 48 hours after the transfer. Fifty eight percent of blastocysts recovered from uterus of asynchronous recipient at 24 hours after transfer and 92.9% of blastocysts recovered from uterus of synchronous recipient were 0-10㎛ of mucin coat thickness. And 11.8% of blastocysts of asynchronized recipients and 7.1% of blastocysts from asynchronized recipients were in 11-20㎛ of mucin coat thickness. When blastocysts were recovered from uterus at 48 hours after transfer, 87.0% of blastocysts from asynchronized recipients and 5.9% of blastocyst from synchronized recipients were in 0-10㎛ of mucin coat thickness. And 76.5% of blastocysts of synchronized recipients and 4.4% of blastocysts from asynchronized recipients were in 11-20㎛ of mucin coat thickness. From these results it is speculated that the low implantation rate of in vitro cultured rabbit blastocysts transferred to oviduct of recipient was caused by high degeneration of the embryo after transfer and inappropriate deposition of mucin coat.

Synovitis, Acne, Pustulosis, Hyperostosis, and Osteitis Syndrome: A Case of Spine, Pelvis, and Anterior Chest Wall Involvement, with Overlooked Plantar Pustulosis

김현수,정소용,이수진,백인운,박정미 대한영상의학회 2017 대한영상의학회지 Vol.76 No.5

Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome is an inflammatory clinical condition with aseptic bone lesions and characteristic skin manifestations. A 63-year-old woman presented with vague musculoskeletal symptoms including chronic buttock pain. The clinical work-up revealed multiple spine and osteoarticular involvement. Multilevel bone marrow edema and cortical erosions involving the spine, asymmetric sacroiliitis, and osteosclerosis of the sternoclavicular joint were consistent with a diagnosis of SAPHO syndrome. Considering SAPHO syndrome in the differential diagnosis, subsequent skin inspection revealed plantar pustulosis. Despite the unique feature of accompanying skin and skeletal lesions, skin lesions could be overlooked if not suspected.

Diagnostic Performance of Diffusion- Weighted Steady-State Free Precession in Differential Diagnosis of Neoplastic and Benign Osteoporotic Vertebral Compression Fractures: Comparison to Diffusion-Weighted Echo-Planar Imaging

신재호,정소용,임정현,박정미 대한자기공명의과학회 2017 Investigative Magnetic Resonance Imaging Vol.21 No.3

Purpose: To evaluate the diagnostic performance of diffusion-weighted steadystate free precession (DW-SSFP) in comparison to diffusion-weighted echo-planar imaging (DW-EPI) for differentiating the neoplastic and benign osteoporotic vertebral compression fractures. Materials and Methods: The subjects were 40 patients with recent vertebral compression fractures but no history of vertebroplasty, spine operation, or chemotherapy. They had received 3-Tesla (T) spine magnetic resonance imaging (MRI), including both DW-SSFP and DW-EPI sequences. The 40 patients included 20 with neoplastic vertebral fracture and 20 with benign osteoporotic vertebral fracture. In each fracture lesion, we obtained the signal intensity normalized by the signal intensity of normal bone marrow (SI norm) on DW-SSFP and the apparent diffusion coefficient (ADC) on DW-EPI. The correlation between the SI norm and the ADC in each lesion was analyzed using linear regression. The optimal cut-off values for the diagnosis of neoplastic fracture were determined in each sequence using Youden’s J statistics and receiver operating characteristic curve analyses. Results: In the neoplastic fracture, the median SI norm on DW-SSFP was higher and the median ADC on DW-EPI was lower than the benign osteoporotic fracture (5.24 vs. 1.30, P = 0.032, and 0.86 vs. 1.48, P = 0.041, respectively). Inverse linear correlations were evident between SI norm and ADC in both neoplastic and benign osteoporotic fractures (r = -0.45 and -0.61, respectively). The optimal cut-off values for diagnosis of neoplastic fracture were SI norm of 3.0 in DW-SSFP with the sensitivity and specificity of 90.4% (95% confidence interval [CI]: 81.0-99.0) and 95.3% (95% CI: 90.0-100.0), respectively, and ADC of 1.3 in DW-EPI with the sensitivity and specificity of 90.5% (95% CI: 80.0-100.0) and 70.4% (95% CI: 60.0- 80.0), respectively. Conclusion: In 3-T MRI, DW-SSFP has comparable sensitivity and specificity to DWEPI in differentiating the neoplastic vertebral fracture from the benign osteoporotic vertebral fracture.

토끼 수정란 체외 배양액의 개발에 관한 연구

임경순,진동일,김대경,김성우,정소용,최화식 한국가축번식학회 1998 Reproductive & developmental biology Vol.22 No.1

This experiment was carried out to improve in vitro development of rabbit one-cell embryos to the blastocyst stage. One-cell rabbit embryos were collected at 19∼20hr after superovulation induction and incubated at 39℃ in 5% CO_(2) for 72hr. In order to find optimum conditions in medium that affects the rabbit embryo's development in vitro, RDH medium which mixed with RPMI1640, DMEM and Ham's F10 was compared with the previously reported mediums (Ham's F10 and RD) for embryo development and cell numbers. Three additives (BSA, taurine and glucose) were tested for the development of rabbit one-cell embryos in vitro. When the embryos were cultured in RDH medium, their development was markedly promoted as compared with Ham's F-10 or RD alone. Glucose exhibited no significant effects on embryo development and cell numbers. BSA appeared to promote transition from morula to blastocyst stage and taurine increased cell numbers of cultured embryos markedly regardless of mediums. BSA and taurine together in RDH medium showed the additive effects on embryo development and cell number.

기초 컴퓨터 언어 교육과정의 설계

김일민(Il-Min Kim),정소용(So-Young Jung) 한국멀티미디어학회 2006 한국멀티미디어학회 학술발표논문집 Vol.2006 No.2

컴퓨터 관련 전공 대학교육에 있어서 가장 기본이 되는 교육 과정이 프로그래밍 언어이다. 현재 많은 대학에서 초급 프로그래밍 언어로서 C를 선택하고 있다. C 언어는 유닉스 운영체제를 작성할 만큼의 다양한 기능을 가지고 있으며 현재 산업계에서도 널리 쓰이고 있는 프로그래밍 언어이다. 그러나 C 언어는 교육용으로 개발되지 않았으며 Pascal이나 Simula와 같이 간결하고 통일된 형식을 지원하지 않는다. 이 논문에서는 초급 프로그래밍 언어 교육에 있어서 C언어가 가지고 있는 문제점을 지적하고, 초급 프로그래밍 언어 교육의 방향을 제시하고자 한다.

HSS STI-CMP적용을 위한이중 패드의 최적화

서용진,박성우,김철복,정소용,이경진,김기욱,박창준 대불대학교 2002 大佛大學校大學院 硏究論文集 Vol.- No.1

As the device geometry shrinks to the deep submicron region, chemical mechanical polishing(CMP) planarization become a more essential technique of advanced ULSI process. Also, CMP process was required for the global planarization of inter-metal dielectric(IMD), inter-level dielectric(ILD) layers and interconnections with free-defect. Especially, the complete global planarization of IMD, ILD and interconnections can be achieved only with the CMP process. However, as the IMD and ILD layer gets thinner, several problems were found in the CMP process. It does have various problems such as dishing effect, torn oxide defects and nitride residues in oxide. So, it leads to severe circuit failure, which affects yield. In this paper, we studied the characteristics of polishing pad, which can apply STI-CMP process for global planarization of multilevel interconnection structure. Also, we investigated the effects of different sets of polishing pad, such as soft and hard pad. As an experimental result, hard pad showed center-fast type, and soft pad showed edge-fast type. Totally, the defect level has shown little difference, however, the counts of scratch was defected less than 2 on JR111 pad. Through the above result, we can select optimum polishing pad, so we can expect the improvement of throughput and device yield.

임신 3 기 경증자간전증 환자를 대상으로 한 저용량 Aspirin의 치료 효과

이영희,이종민,이지성,김용욱,서명덕,정소용 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.7

목적: 자간전증 및 자궁내 태아 발육부전을 예방하기 위한 저용량 aspirin 치료는 임신초기 에 고위험 산모를 대상으로 제안되고 있다. 그러나 임신초기에 고위험 산모를 선별하기 위해 수많은 예측방법이 시도되고 있으나 알려진 선별검사의 한계성 때문에 저용량 aspirin 예방용법은 많은 자간전증 산모를 대상으로 시행하기 어렵다. 이에 본 연구는 고위험 산모가 아닌 경증 자간전증의 증상과 징후가 발현된 임신 3기 의 산모를 대상으로 저용량 aspirin이 중증 자간전증으로의 진행을 감소시키고, 임신 예후를 개선시키는지 연구함으로써 저용량 aspirin이 자간전증으로 진단된 산모를 대상으로 치료효과를 보이는지 알아보고자 본 연구를 시도하였다. 방법: 가천의과대학부속 길병원 산부인과에 경증 자간전증으로 입원한 28주에서 36주사이의 초산모 94명을 대상으로 그중 무작위적으로 48명에게 60 ㎎의 aspirin을 투여하고 나머지 46명을 대조군으로 하여 비교·분석하였다. 결과: 치료군과 대조군의 분만시 평균 임신주수는 36.0±2.7주, 36.7±2.1주이었고 평균 혈압은 수축기 153.13±1, 150.65±1 mmHg, 확장기 101.67±7, 99.78±8 mmHg 이었으며 중증 자간전증으로의 진행은 37.5%, 34.8%로 유의한 차이는 없었다[P$gt;0.05]. 신생아 체중은 각각 2311±733, 2415±505 gm이었으며 5분 아프가 점수는 8.1±2, 8.2±2이었고 태아발육부전은 41.7%, 41.3%로 주산기 예후도 유의한 차이가 없는 것으로 나타났다[P$gt;0.05]. 결론: 경증 자간전증 산모를 대상으로 한 본 연구에서 저용량 aspirin이 중증 자간전증의 진행 및 자궁내 태아발육지연에 대한 예방적 효용을 보이지 않았으므로 저용량 aspirin은 임신 1기 및 2기에 임상증상이 발현되기 수주전에 투여해야 자간전증의 발생을 막고 임신예후를 개선시킬 수 있을 것으로 사료된다. Objectives: Low-dose aspirin therapy is recommended as prophylaxis in women at high risk for preeclampsia. This study made to determine whether low-dose aspirin reduces the number of women who develope to severe preeclampsia, and whether it reduces perinatal mortality and improves birth weights in pregnant women who develope the symptom and sign of the mild preeclampsia. Methods: Ninety-four patients were recruited from the antenatal clinics at Gil medical center with mid preeclampsia at 28 to 36 weeks gestation and were randomized to receive either 60㎎ of aspirin or none. Results: The mean blood pressure values [systolic 153.13±1 vs 150.65±1 mmHg, diastolic 101.67±7 vs 99.78±8 mmHg], the rates of development of severe preeclampsia [37.5% vs 34.8%], the gestational ages at delivery [36.0±2.7 vs 36.7±2.1 weeks], newborn body weights [2311±733 vs 2415±505 gm], 5-minute Apgar scores [8.1±2 vs 8.2±2], and fetal growth retardation[41.7% vs 41.3%] were similar in the low-dose aspirin treated and the control groups. Conclusions: We conclude that low-dose aspirin is not curative in reducing development of severe preeclampsia and in lowering fetal growth retardation, but it is essential to a preventive treatment for high risk patients which, in order to be effective, should be started weeks before clinical signs of preeclampsia are present.

악성 질 흑색종 광범위 국소 질 및 직장 절제술의 시도

서명덕(Myeong Deok Seo),이영희(Young Hee Lee),정소용(So Yong Jung),이종민(Jong Min Lee),박찬용(Chan Yong Park),최유덕(Yu Duk Choi),조현이(Hyun I Cho) 대한산부인과학회 1999 Obstetrics & Gynecology Science Vol.42 No.8

Primary malignant melanoma of the vagina is an exceedingly rare entity, accounting for about 3% of all primary vagina1 malignant tumors and 0.3% of all melanoma. The overall 5-year survival in patients with primary vaginal melanoma is notoriously poor, estimated to be between 13% and 19%. A case of primary malignant melanoma of the vagina treated with the wide local excision of vagina and rectum was presented with a brief review of literature.