약용 식물 추출물의 항산화 활성 검색

정성제,송효남,백남인,이진희,성낙술,이승은 한국응용생명화학회 2004 Journal of Applied Biological Chemistry (J. Appl. Vol.47 No.1

The antioxidant activities of 80% methanol extracts obtained from 118 medicinal plants were tested through the evaluation of DPPH and superoxide anion radicals scavenging activity. Methanol extracts of Sophora japonica (76.9%) and Camptotheca acuminata Dence (50.9%) were found to have more than 50% DPPH radical scavenging activity while those of Perilla frutescens (37.2%), Amomum costatum (34.9%), Prunus ansu (33.2%), Mentha arvensis (32.3%), Serratula koreana (32.2%), Eriobotrya japonica (30.5%), and Artemisia asiatica (30.5%) showed more than 30% scavenging activity. Even though all of the commercial antioxidants didn’t show superoxide anion radical activity, Mentha arvensis (87.7%), Eriobotrya japonica (84.9%), Amomum costatum (82.9%), Camptotheca acuminata Dence (82.1%) showed more than 80% scavenging activity. Mentha arvensis, Eriobotrya japonica, Amomum costatum, Camptotheca acuminata Dence showed strong antioxidative activity in the both DPPH and superoxide anion radical scavenging activities.

약용 식물 추출물의 항산화 활성 검색

정성제 ( Jeong Seong Je ),이진희 ( Lee Jin Hui ),송효남 ( Song Hyo Nam ),성낙술 ( Seong Nag Sul ),이승은 ( Lee Seung Eun ),백남인 ( Baeg Nam In ) 한국응용생명화학회 2004 Applied Biological Chemistry (Appl Biol Chem) Vol.47 No.1

The antioxidant activities of 80% methanol extracts obtained from 118 medicinal plants were tested through the evaluation of DPPH and superoxide anion radicals scavenging activity. Methanol extracts of Sophora japonica (76.9%) and Camptotheca acuminata Dence (50.9%) were found to have more than 50% DPPH radical scavenging activity while those of Perilla frutescens (37.2%), Amomum costatum (34.9%), Prunus ansu (33.2%), Mentha arvensis (32.3%), Serratula koreana (32.2%), Eriobotrya japonica (30.5%), and Artemisia asiatica (30.5%) showed more than 30% scavenging activity. Even though all of the commercial antioxidants didn`t show superoxide anion radical activity, Mentha arvensis (87.7%), Eriobotrya japonica (84.9%), Amomum costatum (82.9%), Camptotheca acuminata Dence (82.1%) showed more than 80% scavenging activity. Mentha arvensis, Eriobotrya japonica, Amomum costatum, Camptotheca acuminata Dence showed strong antioxidative activity in the both DPPH and superoxide anion radical scavenging activities.

Burkholderia(Pseudomonas) cepacia의 황 산화 특성 및 황화 수소 제거

정성제(Sung-Je Jung),이은관(Eun-Kwan Lee),전억한(Uck-Han Chun),윤인길(In-Kil Yoon),박창호(Chang-Ho Park),박승국(Seung-Kook Park) 한국생물공학회 2001 KSBB Journal Vol.16 No.5

다당류 레반의 점도 특성 및 열적 특성에 관한 연구

정성제,송기방,김병용,전억한,이상기 한국산업식품공학회 1999 산업 식품공학 Vol.3 No.3

김치 발효에 관여하는 젖산균으로부터 Bacteriocin의 검색

조재선,정성제,김영목,전억한 경희대학교 부설 식량자원개발연구소 1994 硏究論文集 Vol.15 No.-

Lactic acid bacteria in Kimchi fermentation were tested for inhibitory activity against Gram positive bacteria and Gram negative bacteria. The Lactobacillus brevis (KCCM 35464) was found to produce a antimicrobial substance. It showed relatively wide range of inhibition spectrum against gram positive and gram negative bacteria and maintained the inhibitory activity between pH 4.0 and pH 9.0. The antimicrobial substance was obtained in the stationary growth phase and was purified by gel chromatography. The inhibitory effect of the antimicrobial substance on sensitive bacterial strains was determined by filter paper test. The activity of antimicrobial substance was stable at 75℃. On the basis of its electrophoretic pattern is SDS-PAGE, antimicrobial substance appeared as a single band of 59 KDalton.

독성물질 농도에 따른 Photobacterium phosphoreum의 bioluminescence 변화

정계훈,정성제,이용제,허문석,전억한 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.1

본 연규에서는 발광미생물인 P. phosphoreum을 이용하여 수질 측정용 바이오센서를 개발하고자 free cell과 고정화 세포의 독성물질에 대한 반응성을 조사하였다. 고정화 물질로는 절차가 비교적 간단한 sodium alginate를 사용하였다. Alginate는 발광미생물의 빛 발생 대사를 저해하지 않을 뿐만 아니라 bioluminescence의 투과성이 탁월하였다. 독성물질의 MIC값을 보면 As_2O_3가 400 ppm, phenol이 800 ppm, SeO_2와 CrO_3가 60 ppm으로 나타났다, 독성물질 As_2O_3, phenol, SeO_2, CrO_3를 3가지의 조건 free cell과 Sr-alginate 및 Ca-alginate에 고정화한 세포에 투여하여 반응성을 조사하였다. Free cell이 가장 민감한 반응을 보였으며 free cell과 고정화 세포 모두 독성물질에 대하여 발광량이 농도에 비례하여 감소하였다. 특히, 고정화 세포인 경우 free cell보다 독성물질의 mass transfer에 미치는 영향때문에 민감성은 떨어지지만 직선의 관계식을 나타내었다. Sr-alginate고정화 세포와 Ca-alginate고정화 세포의 독성물질에 대한 반응 양상은 비슷하게 나타났다. 독성물질에 대한 반응분석은 Gamma value로부터 EC_50을 구하여 각 독성물질의 농도와 P. phosphoreum의 발광량과의 상관관계 및 각 독성물질의 독성정도를 산출하였다. Photobacterium phoshoreum was used for the study of bioluminescence response to toxic substances including phenol, As_2O_3, SeO_2, and CrO_3 in view of developing monitoring system. Measurement of inhibition of bioluminescence in P. phosphoreum has been proposed as a sensitive and rapid procedure to monitor toxic substances. The concentration of toxic substance causing 50% light reduction(EC_50) in bioluminescence intensity was determined with free and immobilized P. phosphoreum. The minimum inhibitory concentration(MICs) for bioluminescence emission were found to be 400 ppm for As_2O_3, 800 ppm for phenol, 60 ppm for SeO_2 and 60 ppm for CrO_3, respectively. The linear correlation between Gamma value and the concentration of toxic substances was obtained and EC_50 was calculated from the linear correlation. The free cells were shown to be more sensitive to toxic substances than cells immobilized on Sr-alginate and Ca-alginate, However, the linear regrassion curves were derived form the Sr-alginate immobilized cells indication the immobilization method is a useful tool for monitoring of toxic substances under the more stable condition of bioluminescence.

Photobacterium phosphoreum의 생체발광 유지도에 관한 연구

김현숙,정성제,전억한 한국산업미생물학회 2000 한국미생물·생명공학회지 Vol.28 No.2

P. phosphoreum의 생존과 생체발광도는 온도에 의해 많은 영향을 받는다. 냉동 저장한 세포의 경우 glycerol의 보호작용으로 세포농도와 생균수는 측정기간 동안 일정하게 유지된 반면 생체발광도는 glycerol 첨가 직후 급속히 감소하였으며, 저장 이후에도 감소된 생체발광도가 활성화되지 못하였다. 최적 생육온도인 20℃의 경우 저장 초기 세포가 성장함에 따라 세포수의 증가를 보였으나 일정 시간 이후 세포 분해 현상으로 인하여 생균수 및 세포 집락수의 감소를 나타내었으며, 생체발광도는 저장 3일 이후 소멸되었다. 이와는 대조적으로 4℃에 저장한 세포의 생체발광도는 저장 10일 동안 지속되어 가장 높은 생체발광 유지도를 나타내었으나 장기간 저온 저장으로 인하여 세포가 VBNC 상태에 돌입됨에 따라 총균수와 생균수는 일정한 반면 저장 10일 이후 세포 집락수의 급격한 감소를 나타내었으며, 저장 20일 이후 간균에서 구균으로 세포 형태상의 변화를 나타내었다. 이에 따라 세포 저장 시 접종원의 농도를 달리하여 VBNC 상태와 생체발광도의 관련성을 조사한 결과 VBNC 세포가 증가할수록 생체발광도의 감소를 나타내었다. 따라서 VBNC 세포를 감소시키기 위하여 세포를 고정화하여 저장한 결과 별도의 활성제 없이 실온에서 다시 활성화되어 고정화하지 않은 세포에 비해 2.3배 높은 생체발광 유지도를 나타내었으며, 저온저장에 따른 platebility 소실과 세포 응축현상이 나타나지 않았다. 이러한 결과는 세포의 고정화 방법을 이용하여 4℃에서도 세포의 생존 및 생체발광 유지도를 향상시킬 수 있으며, 동결 건조법의 단점을 보완해 줄 것으로 생각된다. The object of this work is to improve the maintenance of bioluminescence from stored Photobacterium phosphoreum in a view of developing continuous monitoring system for pollutants. The long-term experiments were performed to determine the effect of storage temperature and immobilization on the maintenance of bioluminescence and viability of P. phosphoreum. A naturally luminescent bacterium, P. phosphoreum was starved in 2.5% NaCl solution at 20℃, 4℃, -20℃ and -70℃ for 30 days. In vivo luminescence was measured by luminometry, and total cell concentrations and concentrations of culturable and viable cells were determined by acridine orange staining, dilution plate counting, and direct viable counting, respectively. The bioluminescence emission from cells stored at 4℃ was maintained up to 10 days while those with starved cells at other temperature ranges decreased to background level within 3 days. In terms of viability of cells, concentrations of cells stored at 20℃ were rapidly decreased as a result of cell lysis, leading to a drop in culturable and viable counts while cells stored at 4℃ was shown viable but nonculturable state during starvation. With immobilized cells on strontium alginate, the bioluminescence showed higher maintenance than free cells and decreased with count number of nonculturable cells.

Photobacterium phosphoreum을 고정화하기 위한 Carboxymethylcellulose Sodium Salt [CMC] 담체의 이용성 연구

이용제,정성제,허문석,전억한 한국생물공학회 2000 KSBB Journal Vol.15 No.1

Bioluminescence of Photobacterium phosphoreum has been used for the detection of pollutants in the environment. Immobilization method was used to maintain the stability of bioluminescence of P. phosphoreum. The carboxymethylcellulose was investigated to find out whether it was suitable for the immobilization of P. phosphoreum as a matrix without disturbing the bioluminescence emission. A maintenance of bioluminescence was determined from the P. phosphoreum immobilized on the various concentrations of carboxymethylcellulose. A relatively high bioluminescence intensity was shown with immobilized cells on 1%(w/v) carboxymethylcellulose. The effect of carboxymethylcellulose concentrations on the sensitivity of Crcompounds including $Na_{2}CrO_{4}$, $K_{2}CrO_{4}$, $CrO_{3}$, CrK$(SO_4)_{2}$ and $CrCl_{3}$ to the bioluminescence intensity. The calculated $EC_{50}$ showed that the linear relations between such substances and bioluminesence intensity were established.

산가수 분해법에 의한 레반 올리고당의 제조 및 유산군 생육촉진 효과 연구

강태호,정성제,강순아,강기효,장은경,김승환,김철호,이상기,전억한 한국생물공학회 2002 KSBB Journal Vol.17 No.2

Levan oligosaccoharides and low molecular weight levin were produced from levin by acid hydrolysis and following column chromatography. Levan hydrolysis was progressed proportionally as increased incubation time. In terms of levan hydrolysis reaction, no differences were found from the sources of levan. Optimum hydrolysis conditions for the formation of levan oligosaccharides were, 0.38 M H$_2$S0$_4$; and incubation at 95$\^{C}$ for 4 min. The purified products were determined as the mixture of oligosaccharides (degree of polymerization (DP) of 3-6), Two of lactic acid-producing bacteria, Lactobacillus plantarum KCTC 3104 and Pediococcus pentosaceus KCTC 3507, were studied in vitro for their ability to metabolize levin oligosaccharides. Apparently, the growth of both cells were increased by levin oligosaccharide diet, compared with those of levan diets, suggesting that levan oligosaccharides may be beneficial in selectively growth of lactic acid-producing bacteria.

PEG 분해균주의 분리와 PEG film의 상용성에 관한 연구

이제혁,정성제,이준열,전억한 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-

PEG를 sole carbon과 energy source로서 이용하는 미생물을 자연계에서 분리하였고, PEG의 분자량이 높아질수록 그 분해 미생물의 수가 감소하는 것을 확인하였다. 또한, liquid culture로서 PEG농도를 감소시키는 미생물을 선별하였고, 분해율은 PEG 8000이 약 18.8%였으며 PEG 10000은 약 25.4%인 것으로 조사되었다. PEG film의 제조를 위해 EMAA 및 EAA와의 상용성을 적외선 분광(IR) 스펙트럼을 사용하여 조사한 결과, EMAA와 EAA의 카르보닐기와 PEG의 에테르기와의 강한 수소결합이 형성으로 blend film제조시 상용성이 있는 것으로 확인되었다. Several strains capable of degrading PEGs(Polyethylene Glycols)were isolated and investigated for their biodgradation ability of PEGs. Microorganisms screened for the biodegradation studies were those grown on the PEG used as a sole carbon and energy source. It was known that the number of microorganisms decreased when grown on the high molecular weight of PEG(e.g. 20,000). A liquid culture was carried out with such microorgaisms and resulted in the decrease in PEG concentration meaning that PEG was degraded in the reactor. The biodegradability was found to be about 18.8% for PEG-8000 and 25.4% for PEG-10000, respectively. For the manufacture of biodegradable PEG film, EMAA/PEG and EAA/PEG blending ability was investigated with IR spectrum and showed that it was possible to produce blending film.