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폐암환자의 경기관지 생검조직에서 MAGE 및 GAGE 발현
정만홍(Maan Hong Jung),김지호(Ji Ho Kim),김지혜(Ji Hae Kim),박기룡(Ki Ryong Park),옥철호(Chul Ho Oak),조현명(Hyun Myung Cho),장태원(Tae Won Jang) 대한내과학회 2002 대한내과학회지 Vol.62 No.1
N/A Background: There has been significant pr ogress in the identification of tumor associated antigens. Among the tumor associated antigens, MAGE (melanoma antigen), BAGE, GAGE, PRAME, NY-ESO were named as cancer/testis specific antigens since they are only expressed in the testis or cancer cells. Because of their relative specificity, they have been considered as the appropriate targets for the specific immunotherapy, or the early diagnosis of several cancers. In bronchogenic cancer, these antigens would be useful as a promising candidate in the screening test or immunotherapy. This study was to investigate the expression of MAGE and GAGE genes in the bronchogenic cancer tissues obtained by bronchoscopy. Methods: In five normal bronchial and 26 cancer tissues obtained by bronchoscopic biopsy from 26 bronchogenic cancer patients, total cellular mRNA was extracted. Then RT PCR was run in 35 cycles, with two different kinds of primers designed to detect the several subtypes of MAGE DNA simultaneously and the similar process to detect GAGE DNA was also done. Concurrently, DNA sequencing of the isolates was done in portion to prove the isolates are cloned MAGE and GAGE DNA. With probes confirmed by DNA sequencing, the isolates were reevaluated by Southern blotting. Then the expression of MAGE or GAGE in the bronchogenic cancer tissues was evaluated by the tissue types and clinical staging. Results: In the five controls, MAGE or GAGE was not detected in any specimen and beta actin was not expressed in 4 cases, suggesting the specimen might be too small to detect beta actin by 35 cycles of PCR. In the 26 cancer tissues, the expression rate of MAGE and GAGE was 42.3% (11/26) and 42.3% (11/26) respectively and MAGE or GAGE were expressed in 17 cases (65.3%). Neither clinical staging nor tissue types were associated with the expression of MAGE or GAGE. Bet a actin was not detected in 11 cases of cancer specimen, but MAGE or GAGE were expressed in 10 cases of them. Conclusion: Using these primers in detection of MAGE or GAGE genes in the bronchoscopic cancer tissues obtained by bronchoscopy biopsy tissues seems to be effective or complimentary method in screening of bronchogenic cancer patients, who would be the candidate for the possible immunotherapy.(Korean J Med 62:58-68, 2002)
옥철호,정만홍,장태원,신성훈 고신대학교(의대) 고신대학교 의과대학 학술지 2008 고신대학교 의과대학 학술지 Vol.23 No.3
Background : Many tumor specific antigens have been studied for tumor diagnosis and immunotherapy. Among tumor specific antigens , Melanoma antigen gene(MAGE) is exclusively expressed in the testis or malignant cells. We investigated MAGE expression in pleural fluid to differentiate malignant from benign pleural effusion. And the results were compared with those of cytologic examination and tumor maker(CEA) Method : we studied 56 patients with pleural effusions to the Kosin University Gospel Hospital between April 2002 and April 2004( 31 men and 25 women: mean age, 56 years). Expression of MAGE was examined by RT-PCR method using a commercial kit. Tumor maker (carcinoembryonic antigen[CEA]) in pleural fluid was determined by immunoassay. Thirty six patients were proven to have malignant pleural effusion by cytology and pleural biopsy, while 20 patients had benign pleural effusion. Results : MAGE was not expressed in 20 patients with benign pleural effusion, while it was expressed in 23 patients (63.8%) of 36 patients with malignant effusion. The sensitivity of cytologic examinations were 50%. At 100% specificity, a pleural CEA > 50ng/mL had 20% overall sensitivity. The combination of cytology and MAGE reached 84% sensitivity, whereas the combined use of the cytology and tumor marker and MAGE increased sensitivity up to 92%. More than one third of cytology-negative malignant pleural effusion had expression of MAGE. Conclusion : Expression of MAGE in pleural fluid would be a useful and complementary method for differential diagnosis between benign and malignant pleural effusion.