사슴의 내부기생충 감염에 관한 조사연구

윤화중,정길생,Yoon Hwa-Joong,Chung Kil-Saeng 대한수의사회 1983 대한수의사회지 Vol.19 No.7

The authors made a survey on the species and distribution of internal parasites in domesticated deer. Total 75 specimen which collected from Seoul City, Gyeonggi Do, Kangwon Do and Chung-Nam Province were carried out for the actual condition of parasitic

선진 유럽유기농업의 환경보전 기능과 안전농산물 생산 - 한국유기농업의 발전을 위한 농업정책적 제안 -

정길생 ( Chung Kil-saeng ),손상목 ( Sohn Sang-mok ),이윤건 ( Lee Yoon-gun ) 한국유기농업학회 1996 韓國有機農業學會誌 Vol.5 No.1

In Korea there is still no basic standard for organic agriculture and organic farmers in Korea do not follow the minimum requirements of IFOAM basic standard. Most of them just practice the organic agriculture applying organic fertilizer, commercial seed without legume, rotation and green manure. But they believe this system is a absolutely environmental friendly agricultural system and it produce a safe agricultural product since they are not aware of the basic standard of organic agriculture at all. The overuse of organic fertilizer by some organic farmer have caused some severe problems such as NO₃ and P₂O₅ accumulation in soil profile showed the potential risk for nitrate and phosphate leaching. In the paper, it is discussed on the the environmental friendly function and the safe vegetable production by european organic agriculture which keeps the internationally recognized basic standards of organic agriculture. Therefore it is strongly recommended that korean organic farmer have to follow the IFOAM basic standard in order to practice the environmental agriculture and produce the safe food. And it is also necessary to introduce to Korea the basic standard of organic agriculture which coincides with IFOAM’s and Codex of FAO/WHO immediately if they really want to practice an organic agriculture in the country.

수정란이식의 산업화 방안

정길생 ( Kil Saeng Chung ) 한국축산학회 1983 한국축산학회지 Vol.25 No.7

Prostaglandin F2 - Alpha 및 Nor - Adrenalin 투여가 유우의 발정주기와 혈중 Steroid Hormone 함량에 미치는 영향

정길생 ( Kil Saeng Chung ) 한국축산학회 1979 한국축산학회지 Vol.21 No.5

This experiment was carried out to develop a new luteolytic reagent and its effective administration technique which could be used for the synchronization of estrus cycle in dairy cattle. Five miligram of prostaglandin F₂x and 2 ㎎ of nor-adrenalin were administrated by way of intravulvosubmucous, respectively, and the induction of estrus and the rates of synchronization were observed. The changes in the concentration of steroid hormones such as progesterone, estradiol-17β and testosterone in blood plasma of dairy cattle prior to and after administration of the reagents were also estimated. The results obtained in this experiment were summarized as follows; 1) Standing estrus was induced in 8 cows out of 10 cows within 2 to 4 days after administration of prostaglandin F 2α injected by way of intravulvosubmucous at 10 days after natural ovulation. 2) Six cows out of 10 cows were resulted in standing estrus within 5 to 6 days after administration of nor-adrenalin via intravulvosubmucous at 10 days after natural ovulation. 3) The concentrations of steroid hormens in blood plasma of dairy cattle responded to the administration of prostaglandin F2α and nor-adrenalin were determined. The Plasma progesterone levels had fallen from 7.615±0.81ng/㎖ and 6.242±182ng/㎖ to 2.839±0.341ng/㎖ and 1.583±0.520 ng/㎖, respectively, within 6 hoots after administration of prostaglandin F2α and noradrenalin and then declined to a baseline level within 24 hours. 4) The concentration of plasma estradiol-17β rose to a peak level, 55.7±13.19 pg/㎖, within 3 days after injection of prostaglandin F2α and 60.3±8.15 pg/㎖ within 5 days after injection of nor-adrenalin, respectively, and the estrus were induced at the time when estradiol-17β levels pose to the peak, respectively. 5) The peak levels of estradiol-17β during the estrus induced by the administration of prostaglandin F2α or nor-adrenalin were lower significantly than those of estradiol-17β during the natural estrus, respectively. 6) Testosterone concentration increased during the 3 days preceding estrus and attained peak values, 59.4±9.73pg/㎖ and 55.8±6.14pg/㎖, respectively, 48 hour before the onset of estrus induced by prostaglandin F2X or nor-adrenalin. 7j Progesterone concentrations in blood plasma of dairy cattle not responded to the administration of prostaglandin or nor-adrenalin were also fallen sharply within 6 hours after administration of the reagents but no levels below 1.0 ng/㎖ were observed. 8) From the results above mentioned it could be concluded that nor-adrenalin was as effective reagent as prostaglandin F2α for the synchronization of estrus cycle in dairy cattle and that intravulvosubmucous injection method was the most effective technique for the administration of the reagents.

포유동물배의 미세조작 기술

정길생(Kil Saeng Chung) 건국대학교 유전공학연구소 1984 건국 유전공학지 Vol.1 No.-

한국재래산양의 정액성상에 관한 연구

강석원,정길생 ( Seok Won Kang,Kil Saeng Chung ) 한국축산학회 1976 한국축산학회지 Vol.18 No.2

This experiment was carried out to clarify the seasonal changes in general characteristics such as semen volume, sperm concentration, sperm motility, sperm abnormality and pH of the semen collected from Korean native goats by the method of electrostimulation. The results obtained in this experiment were as follows: 1. Semen volume per ejaculation was increased with decreasing daylight and the reverse was true fur the incensing daylight; average semen volume per ejaculation of November, December and January was 0.95㎖ and that of July, August and September was 0.50㎖. Significant difference (P$lt;0.05) between those two volumes was observed: 2. Seasonal changes in sperm concentration was reciprocal to those of semen volume. Average sperm concentration of July, August and September was 16.02×10^8/㎖, and that of November, December and January was 9.86×10^8/㎖ and there was also significant difference (P$lt;0.05) between those two concentration. 3. Sperm motility was changed in parallel with the variation of semen volume per ejaculation; average sperm motility of November, December and January was 83.30 and that of July, August and September was 55.10 as motility index, and significant difference (P$gt;0.05) between those two values was observed. 4. Sperm abnormality was also changed in parallel with semen volume; average percentages of abnormal spermatozoa during November, December and January were higher than those of the other months. 5. The pH of ejaculated semen was ranged from 6.80 to 7.20 throught the year and no remarkable variation acording to season was observed. 6. Significantly high (P$lt;0.01) negative correlations between semen volume and sperm concentration, sperm concentration and sperm motility, sperm concentration and sperm abnormality was observed. And there was also positive correlation between semen volume and sperm motility, semen volume and sperm abnormality, semen volume and pH, and sperm motility and pH. 7. It was clarified by those results that the reproductive activity of Korean native goat was activated by the increasing daylight began from November to January in Korea.

경기지방에 있어서 유우의 유방염에 관한 조사연구

손봉환,정길생 ( Bong Whan Shon,Kil Saeng Chung ) 한국축산학회 1978 한국축산학회지 Vol.20 No.3

A total of 285 quarter milk samples presented by cow owners and field veterinarians from 137 dairy cow s of 41 herds were examined for mastitis and the following results were obtained. 2. The highest percentage of quarter infected by mastitis was observed in August and other month were low. 3. It was found that 89 quarters (3.22%) were acute mastitis, 791 quarters (67.00%) were chronic mastitis and 5 quarters(1.78%) were mastitis before parturition. 4. The main causative organisms of the mastitis were Staphylococcus spp. (39.63%), Streptococcus spp. (38.22%). 5. The majority of causative organisms were very resistant to penicillin (85.4%) and streptomycin(78.1%). Most of the other drugs were highly effective as inhibitor for the most causative organisms in vitro.

한우의 수정란 이식에 관한 연구 제1보 . GTH 단독투여에 의한 한우의 쌍태 유기

고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4

For the studies of twin birth induction on Korean native cattle, the following processes were taken: first, PMS were injected subcutaneously to promote the growth of follicles, then HCG were injected into the vein for the superovulation and the artificial insemination were performed for conception. The results were gained as follows: 1. Average duration from PMS administration to onset of estrus was 3.8 to 4.7 days and no differences due to dosage of PMS were observed. 2. Duration of induced estrus was ranged from 3.3 to 4.6 days and the length of induced estrus cycle was shortened than that of normal estrus. 3. The most grown follicles (5.3 ∼ 5.5) were observed in the cattle administrated with 2,000 IU of PMS and 1,000 IU of PMS was considered to be the optimum dosage for the limited ovulation. 4. Ovulation rate was declined when the PMS dosage was increased more than 1,000 IU. 5. The highest rate of twin birth (35.7%) was obtained from the cattle administrated with 1,000 IU of PMS and the next was the cattle administrated with 2,000 IU and 750 IU of PMS, respectively.

한우의 수정란 이식에 관한 연구 제1보 . GTH 와 PGF 2α 투여에 따른 난소반응

고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4

For the induction of follicle development and heat, PGF₂α was injected intramuscularly two days after PMS was injected by subcutaneous or intra-ovary methods on multiparous cows during of 9-13 days of the estrus cycle. After the above treatments, intravenous HCG injection for the superovulation was followed. The responses of ovaries were as follows: 1. Average duration from PMS administration to the onset of estrus was 5 days and the duration was shortened with the increasing dosage of PMS. 2. Duration of induced estrus was ranged from 2.3 to 4.8 days and the duration of estrus was shortened with the decreasing dosage of PMS. 3. Number of follicle obtained from the cattle treated with 4,000, 3,000, 2,000, 1,000 and 500 IU of PMS were clarified to be 11.4, 11.5, 7, 2.3 and 2.5, respectively, after 4.8 - 5.2 days of PMS administration. 4. Observing the results of slaughter, ovulation rates of the cattle treated with 4,000, 3,000, 2,000, 1,000, and 500 IU of PMS were 16.3, 35.6, 60.0, 65 and 75%, respectively. 5. The numbers of degenerated, or bled follicle were increased with the increase of PMS dosage.

한우의 수정란 이식에 관한 연구 제3보 . 수정란의 비외과적 채취와 이식

고광두,정길생,이기만 ( Gwang Du Goh,Kil Saeng Chung,Ki Man Lee ) 한국축산학회 1981 한국축산학회지 Vol.23 No.4

For the purpose of obtaining the artificial twin birth in the transplanted Korean native cows, non-surgical obtaining of the fertilized eggs and their transplantations were performed. The results were as follows: 1. Average six eggs per donor were ovulated and 65% of them were recovered by non-surgical technique. 2. Eighty two percent of eggs recovered were found to be morphologically normal and were at 4-32 cell stage, respectively. 3. Seventy five percent of cattle treated with prostaglandin F₂α were synchronized in their estrus within 4 days after administration. 4. No twin fetation was obtained from the recipients which received fertilized eggs.