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정근일,강원화,이정아,신동하,배경숙,박호용,박희문,Jeong, Keun-Il,Kang, Won-Hwa,Lee, Jung-Ah,Shin, Dong-Ha,Bae, Kyung-Sook,Park, Ho-Young,Park, Hee-Moon 한국미생물학회 2010 미생물학회지 Vol.46 No.1
Coenzyme Q10 (CoQ10)은 전자전달계에 필수적인 요소로 질병치료 및 완화에 도움이 되어 산업 의학적으로 그 활용도가 넓어지고 있다. 본 연구에서는 새로운 CoQ10 생산균주를 선별하기 위하여 quinone 분석 결과 CoQ10을 함유하는 것으로 확인된 8종 미생물의 생장특성과 CoQ10 생산능을 1차 조사하여, 세균류인 Paracoccus denitrificans KCTC 2530과 Asaia siamensis KCTC 12914를 대량배양을 통한 CoQ10 생산에 유리한 특성을 갖는 균주로 선별하였다. 이들 세균류의 생장 및 CoQ10 생산의 최적조건을 플라스크배양으로 조사한 결과, M81 배지를 기반으로 하여 탄소원으로는 4% fructose, 질소원으로는 2% yeast extract가 가장 좋은 것으로 조사되었으며, 배양온도는 $30^{\circ}C$, 배지의 최적 pH는 P. denitrificans KCTC 2530의 경우 pH 6.0, A. siamensis KCTC 12914의 경우 pH 8.0으로 조사되었다. 이를 바탕으로 2 L fed-batch culture를 수행한 결과, P. denitrificans KCTC 2530은 1 L 당 $14.34{\pm}0.473$ mg, A. siamensis KCTC 12914는 $12.53{\pm}0.231$ mg의 CoQ10을 생산하였다. Coenzyme Q10 (CoQ10) is an essential lipid-soluble component of membrane-bound electron transport chains. CoQ10 is involved in several aspects of cellular metabolism and is increasingly being used in therapeutic applications for several diseases. Despite the recent accomplishments in metabolic engineering of Escherichia coli for CoQ10 production, the production levels are not yet competitive with those by fermentation or isolation. So we tested several microorganisms obtained from the KCTC of Biological Resource Center to find novel sources of strain-development for CoQ10-production. Then we selected two strains, Paracoccus denitrificans (KCTC 2530) and Asaia siamensis (KCTC 12914), and tested to optimize the CoQ10 production conditions. Among the carbon sources tested, CoQ10 production was the highest when fructose was supplied about 4% concentration. Yeast extract produced the highest CoQ10 production about 2% concentration. The highest CoQ10 production was obtained at pH 6.0 for P. denitrificans and pH 8.0 for A. siamensis. And two strains showed the highest CoQ10 production at $30^{\circ}C$, but the highest DCW was obtained at $37^{\circ}C$. In the fed-batch culture, P. denitrificans yielded $14.34{\pm}0.473$ mg and A. siamensis yielded $12.53{\pm}0.231$ mg of final CoQ10 production.
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이성희(Lee, Sung Hee),정근아(Jeong, Keun Ah) 한국육아지원학회 2020 육아지원연구 Vol.15 No.4
본 연구의 목적은 만 5세 유아를 대상으로 유치원에서의 노래 부르기를 어떻게 인식하고 있는지 알아봄으로써 2019 개정 누리과정의 음악교육에 대한 방향 및 교사 역할을 모색해보고자 하였다. 이를 위해 D시의 4개 유치원 만 5세 유아 67명을 대상으로 4-6명씩 14집단으로 구성하여 집단 면담을 실시하였고, 연구 일지와 관찰일지를 수집하였다. 집단 면담은 유아를 대상으로 소그룹 형태의 면담을 계획하였고 유치원에서의 노래 부르기에 대한 경험과 생각을 담은 내용으로 질문하였으며 유아의 답변을 녹음하였다. 녹음자료를 전사하고 자료 수집 내용을 분석한 결과는 다음과 같다. 첫째, 연구에 참여한 유아들은 유치원에서의 노래 부르기를 나를 표현하는 놀이 그 자체로 인식하였다. 둘째, 유아들은 노래 부르기를 공감과 소통의 매개체로 인식하였다. 셋째, 유치원에서 부르는 노래를 일상 속 공동체 생활도구로 인식하였다. 넷째, 학습을 위한 유치원 활동으로 인식하였다. 본 연구를 통해 교사가 2019 개정 누리과정을 토대로 노래 부르기를 할 때는 유아들의 노래 부르기에 대한 이해를 바탕으로 유아가 자유롭게 표현하는 노래와 놀이경험으로 통합되는 과정을 탐색하고 놀이와 활동을 연계하여 지원할 수 있어야 함을 시사한다. The purpose of this study was to explore five-year-olds’ perspectives of singing in kindergarten, seeking the direction and role of teachers in music education under the revised 2019 Nuri Curriculum. 67 children aged five in four kindergartens in D city were studied. Group interviews with four to six children were conducted, and research logs and observation journals were collected. In the group interview, children’s experiences and thoughts about singing in kindergarten were asked, and their responses were recorded and transferred. The results of the data analysis are as follows. First, the participant children perceived singing in kindergarten as the play itself that expresses oneself. Second, children recognized singing as a medium of empathy and communication. Third, the songs sung in kindergarten were recognized as tools for daily community life. Fourth, singing was recognized as a learning activity. This study suggests that when teachers guide singing activities based on the 2019 revised Nuri Curriculum, they should understand the process of children’s freely expressed songs being integrated into the play experience, considering children’s perspectives on singing, and thus support their learning by linking play and activities.
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정근(Keun Chung),김정배(Jeong Bae Kim),민헌기(Hun Ki Min),김영민(Young Min Kim),노영수(Young Soo Rho) 대한두경부종양학회 1998 대한두경부 종양학회지 Vol.14 No.1
Objectives: Minimal improvement in the long-term survival of head and neck cancer(HNC) patients has occurred despite a multitude of advances in the control of loco regional disease and a second primary malignancy(SPM) contribute to the continued poor prognosis for the HNC patients. This study was performed in order to identify the clinical characteristics of SPM in the HNC patients. Materials and Methods: The medical records of 354 patients of head and neck squamous cell carcinoma that were followed up after initial treatment during the period of 1987 through 1994 were reviewed. This study examines the medical records of 354 patients with squamous cell carcinoma of the head and neck, of whom 26 subsequently developed a second neoplasm. Results: The actuarial SPM rate was 7.3%, and median time to presentation for the SPM was 26.8 months. The SPM were more likely to occur in male patients who had oral cavity index tumors. Patient whose index tumor was small at diagnosis had a greater chance of developing a second tumor as did those with no cervical lymph node metastases to the neck. Initial treatment modality was not associated with an increased risk of developing a second tumor. The commonest sites for the SPM were the lung and other head and neck area. The 3-year survival for patients who developed a secondary tumor from the time of its diagnosis was 27.8%. Conclusion: The SPM in the head and neck cancer patients are not uncommon and early detection of the SPM will contribute to increase the long-term survival of HNC patients.
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