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Magnolol 및 Honokiol의 항생제와의 병용 효과
정경수,이수나,김영호,배기환,Chung, Kyeong-Soo,Lee, Soo-Na,Kim, Young-Ho,Bae, Ki-Hwan 한국생약학회 2000 생약학회지 Vol.31 No.4
Antibacterial activities of magnolol (MGL) and honokiol (HKL) in combination with four representative antibiotics-amoxicillin (AMPC), oxytetracyclin (OTC), gentamicin (GM) and chloramphenicol (CAP)-were evaluated against four bacterial strains. When tested by disk-plate method, five out of eight combinations such as HKL-AMPC, HKL-CAP, MGL- AMPC, MGL-OTC, and MGL-CAP showed additive to synergistic interaction against gram- negative bacterium Salmonella typhimureum. Of these, MGL-AMPC combination turned out to be antagonistic against Sarcina lutea and Bacillus thurungiensis. Against these two grain-positive bacteria, only HKL-GM combination showed additivity to synergism. All the other combinations showed no interactions. Despite these results, however, no synergism was observed in checkerboard titration assay.
눈꽃동충하초 (Paecitomyces tenuipes DGUM 32001) 균사배양물로부터 분리한 단백다당체 PJ-4의 in vitro 면역촬성
정경수(Kyeong Soo Chung),이지선(Ji Seon Lee),김용해(Yong Hae Kim),한영환(Young Hwan Han),이만형(Mann Hyung Lee) 대한약학회 2002 약학회지 Vol.46 No.3
In the previous report, we described the in vivo antitumor activity of Pl-4, a protein-polysaccharide fraction prepared from the culture filtra1e of an insect-born fungus, Paecilomres tenuipes DGUM 32001. In the present study we elucidated the immunomodulating activity of PJ-4 on the BALB/C mousse splenic Iymphocytes using flow cytometrical techniques. As a result, PJ-4 was found to stimulate the lymphocytes not only to form Iymphoblasts but also to express CD25 (IL-2 receptor u chain) molecule, which is well known as a T cell activation marker. More Interestingly, its T cell stimulatory activity was more strongly exerted on CD8+ T cells than on CD4+ T cells. All these data suggest that PJ-4 exerts its antitumor activity at least partly through stimulation of T cells which play major roles in the cell-mediated immune system.
큰눈물버섯 (Psathyrella velutina)으로부터 분리한 단백다당체 PVP의 항암 ·면역활성
정경수(Kyeong Soo Chung),이지선(Ji Seon Lee) 대한약학회 2001 약학회지 Vol.45 No.6
A protein-polysaccharide fraction of a Korean wild mushroomwas psathyrella velutina, PVP, was prepared and its antitumor immunomodulatory activity was investigated. When PVP was administered once daily for seven days from day 1 to day 7 into male ICR mice implanted with 1 ×105 cells of sarcoma 180 tumor cells into the peritoneum on day 4, it inhibited the growth of sarcoma 180 cells by 92.8%. In XTT assay; PVP also exherted in vitro anti-proliferation activity on U-937, a human monoblastoid cell line, as well as sarcoma 180 cells. PVP showed marked stimulatory activity on the immune system in that it induced the accumulation of PEC (the stimulation index, Sl=4.90 at 100 mgHg), stimulated the BALB/C mouse splenic Iymphocytes to form Iymphoblasts (Sl=5.75 at 100mcg/ml), and upregulated the expression of CD25 molecules. All these results strongly support that PVP exhorts its antitumor activity through stimulation of the immune system as well as antiproliferative activity on the tumor cells.
Phellinus linteus 균사 배양물로부터 분리한 단백다당체 Kp의 항암활성
정경수(Kyeong Soo Chung),김신숙(Shin Sook Kim),김희수(Hee Soo Kim),한만우(Man Woo Han),김병각(Byoung Kak Kim) 대한약학회 1994 약학회지 Vol.38 No.2
A protein-polysaccaride fraction Kp(53.9% polysaccharide, 14.2% protein) was separated from the shake-cultured mycelia of a basidiomycetous fungus, Phellinus linteus, and its antitumor activity against sarcoma 180 in ICR mice was investigated. When administered after the tumor implantation, Kp exerted antitumor activity by inhibiting the growth of the sarcoma 180 solid tumor by 71.5% and increasing the life span of the sarcoma 180 ascitic mice by 51.5% at 100 mg/kg. In pretreatment tests, in which Kp was administered once daily for 9 days before the tumor implantation, Kp inhibited the growth of the solid and ascites form of sarcoma 180, respectively, by 35.4% and by 80.3% at 100 mg/kg. However, Kp showed no in vitro cytotoxic activity against a murine leukemia L1210 and a human gastric tumor SNU.1 upto the concentration of 200 mcg/ml. From these results, it is clear that the antitumor activity of Kp is exerted through its immunomodulating activity on the host''s immune system.
한국산 담자균류의 배양균사에 함유된 항암성분에 관한 연구
정경수(Kyeong Soo Chung),김병각(Byong Kak Kim) 한국생약학회 1982 생약학회지 Vol.13 No.4
To develops new antitumor constituents from higher fungi, eight species of edible basidiomycetcs were shake-cultured and investigated for antitumor constituents using sarcoma 180 as a tumor cell and ICP mice as test animals. Among the tested species, Pluteus cervinus, Hydnum repandrm, Laccaria laccata, and Pleurotus pulmonarius were recognized to produce antitumor components and P. cervinus. was selected for further study. Four fractions were made from the mycelia and three from the culture filtrate of P. cervinus. Fach fraction was tested for its antitumor activity. The most active fraction was the water soluble fraction obtained from the hot water extract of the mycelia. The tumor inhibition ratio of this fraction were 73.9%, 86.3%, and 87.8%, when administered, i.p., at the dose level of 10㎎, 20㎎, and 50㎎/㎏/day for ten days. This antitumor fraction was subjected to gel filtration on Sephadex G-200 to be separated into two fractions. The higher molecular weight fraction which showed an anthrone positive sharp peak on Sephadex G-204 was fund to be active of the two and tenatively named cerviran A. When chemically assayed, cerviran A was found to be a protein-Bound polysaccharide. The polysaccharide moiety was composed of five sugars, and the protein moiety, 17 amino acids.
긴꼬리말불버섯(Lycoperdon pedicellatum)의 항암 면역활성
정경수(Kyeong Soo Chung),김진향(Jin Hyang Kim) 대한약학회 2000 약학회지 Vol.44 No.5
Protein-polysaccharide fractions separated from nine Korean wild mushrooms were subjected to an in vitro screening test for lymphoblastogenic activity. Of these, PPLP, the protein-polysaccharide fraction of Lycoperdon pedicellatum, showed the most potent activity, and were further investigated for its antitumor activity. When intraperitoneally injected into ICR mice once daily for six days at a dose of 30mg/kg, PPLP strongly inhibited the growth of sarcoma 180 tumor cells, showing the inhibition ratio of 97.6%. PPLP also showed in vitro inhibitory activity on sarcoma 180 or leukemia L1210 at the concentration of 500mcg/ml or higher. These results strongly suggest that PPLP might exert its antitumor activity through immunostimulation as well as inhibitory activity on the tumor cells.
영지버석(Ganoderma lucidum) 생장점으로부터 분리한 단백다당류의 면역활성
정경수(Kyeong Soo Chung),김상범(Sang Bum Kim),정수현(Soo Hyun Chung) 대한약학회 1997 약학회지 Vol.41 No.1
Two protein-polysaccharide fractions, GLA and GLB, respectively, were prepared from the pileus of the fully grown carpophores and the tips of the growing carpophores of Ganoderma lucidum. At a dose of 100mg/kg/day ip, GLA and GLB inhibited the growth of sarcoma 180 solid tumor in ICR mice by 56.3% and 81.8%, respectively. In a flow cytometric (FCM) analysis, GLA and GLB enhanced the formation of lymphoblasts of BALB/c, splenic leukocytes at a concentration of 100 mcg/ml, by 38.3% and 61, 3%, respectively. When ip injected into ICR mice, GLB exerted anti-leukopenic effect against cyclophospamide (75mg/kg, ip) in that the leukocyte counts of the peripheral blood of the normal and the cyclophosphamide-treated mice. respectively. was (11.1 +/- 3.8) x 103 and (4.0 +/- 1.8) x 103, while the GLB-cyclophosphamide treated mice showed a leukocyte count of (10.8 +/- 5.1) x 103 CELLS/mcl. These results suggest that GLB is a promising candidate for an effective, cancer immunotherapeutic agent.
솔잎 수액 증류액의 암세포주에 대한 in vitro 세포독성
정영진(Young-Jin Chung),배명원(Myung-Won Bae),정명일(Myoung-Il Chung),이지선(Ji-Seon Lee),정경수(Kyeong-Soo Chung) 한국식품영양과학회 2002 한국식품영양과학회지 Vol.31 No.4
한국산 솔잎을 가압ㆍ압착하여 얻은 수액을 증류한 솔잎 수액 증류액의 각종 암세포주에 대한 in vitro 세포독성을 시료액대비 10배, 20배, 40배 희석군과 대조군에 대해 XTT법으로 실험한 결과, 쥐 백혈병 세포주인 L1210에 대해서는 76~89%, 쥐 육종암세포인 sarcoma 180에 대해서는 61~90%의 세포성장 억제효과를 나타내었다. 또한 인체의 monocyte-like cancer cell인 U937에 대해서는 56~81%, 인체 유방암 세포주인 T47D와 MDA-MB-231에서는 각기 12%, 또 다른 유방암 세포주인 MH7A에서는 64%, 인체 간암 세포주인 SNU-354에 대해서는 72%의 높은 세포 증식 억제효과를 나타내었다. 이로써 본 연구 시료인 솔잎 수액 증류액은 쥐 백혈병 세포주인 L1210, 쥐 육종암세포인 sarcoma 180, 인체 monocyte-like cancercell인 U937, 인체 유방암 세포주인 MH7A, 인체 간암 세포주인 SNU-354에 대해 강한 세포독성을 갖는 것을 알 수 있었다. 이와 함께 솔잎 수액 증류액의 암세포에 대한 독성효과는 솔잎의 처리과정에 따라 다를 수 있으며, 또한 동일한 솔잎 수액 증류액의 농도에서도 암세포주 종류에 따라 세포독성정도가 다름을 알 수 있었다. 따라서 최적 투여 농도와 적용 암세포주를 찾을 경우 새로운 항암제로 개발될 수 있음을 제시하였다. This study was performed to examine the cytotoxic effects of the distilled pine-needle extracts against several cancer cell lines. First, cell lines including mice leukemic cancer cell line (L1210), sarcoma 180 and human monocyte-like cancer cells (U937) were tested using XTT methods in vitro. Pine-needle extracts were prepared by pressing the pine needles and distilling it at below 98℃ and then added to the growth medium in a final dilution of 10, 20, and 40 times. Growth of three kinds of cancer cells was significantly inhibited by more than 50% with the addition of the extracts. Fifty six to seventy six % of inhibition was shown with the 40 times dilution of the extracts. Greater inhibition was achieved with the 20 times dilution (81~90%) and the 10 times dilution (77~89%) of the extracts. Next, other human cancer cell lines including 3 kinds of breast cancer cell lines (T47D, MDA-MB- 231 and MH7A) and one hepatoma cell line (SNU-354) were tested with the 20 times dilution of the extract. T47D and MDA-MB-231 cell lines showed lower inhibition (12%) with the addition of the extract. However, MH7A and SNU-354 cell lines showed 64% and 72% inhibition with the extract, respectively. These results suggest that the distilled pine-needle extracts have strong cytotoxic effect on certain cancer cell lines and the intensity of the effect may vary depending on the process of the pine needle.