문학텍스트를 활용한 문화소통 능력 향상을 위한 교육 방안 연구

전영희(Chun, Young-Hui),정희영(Jeong, Hee-Young) 대한일어일문학회 2018 일어일문학 Vol.79 No.-

This research is an educational bill study to try cultural lessons utilizing cultural text different from conventional cultural education in which cultural education has concentrated on comparative cultural knowledge transmission in foreign language education. Therefore, the first thing I noticed is the so-called "thinking text" that learners estimate various phenomena with information gathered by themselves, unlike before. What I chose as "thinking text" in this study is the narrative and myth of both Japan and South Korea. The reason for choosing this is that the first one needs to pay attention as universality beyond cultural boundaries, specialty related to the identity of each group, narratives with this conflicting nature and texts of myths as "thinking text" Because there is. The second is to use schema for "new information" called Japanese literature text. In other words, as a way to utilize the learner`s "old information" schema as a way to understand the "new information" called "Kojiki", which attracted attention as a text to think about to foster cultural communication skills related to Japan, I introduced a myth and tale. Classes were divided into a lecture ceremony and a discussion ceremony. Lecture ceremony classes were divided into two, interpretation of text and comparison with similar myth. The discussion ceremony class also parallels the comparison and analysis discussion lesson and the class introducing the scamper technique. The part to pay attention to is the class introducing the scamper technique. It is because we were able to confirm the part that this research is pursuing, that is, learners are thinking a wide range. It also shows understanding of the contents of the lesson that I can not feel any hindrance to digesting and describing knowledge in my own language. It seems that this suggests that cultural education with the goal of nurturing the ability of cultural communication in the future should be oriented.

폐색근위부 및 원위부의 근육세포에서 허혈후 재관류시 나타나는 Super oxide dismutase 의 활성변동에 대한 연구

백두진(PAIK Doo-Jin),양성범(YANG Sung-Bum),안동춘(AHN Dong-Choon),전영희(CHUN Young-Hee),장유경(CHANG You-Kyung),정호삼(CHUNG Ho-Sam) 대한체질인류학회 1997 해부·생물인류학 (Anat Biol Anthropol) Vol.10 No.1

골격근에서 허혈이 지속되면 근육세포 ATP의 대사가 일어나 hypoxanthine 이 생성되고, 사립체에서 발생된 반응성 신소기가 세포악의 변화를 일으켜 세포내 칼슘농도가 증가되면 칼슘의존성 단백질 분해효소의 작용으로 골격근 세포와 혈관내피세포에서 xanthine dehydrogenase가 xanthme oxidase로 전환된다. 허혈상태에서 재관류로 산소가 공급되면 xanthme oxidase와 hypoxanthme의 반응으로 반응성 산소기를 형성하여 근육의 손상이 일어나는 것으로 알려져 있다. 이러한 반응성 산소기의 산화작용에 대처하기 위하여 세포내에는 SOD (superoxlde dlsmutase), catalase등의 항산화효소가 분포하며, 허혈후 재관류시 형성된 반응성 산소기인 superoxlde 기는 SOD의 작용에 의해 대사되는 것으로 알려져 있다 한연, 허혈후 재관류된 근육에서 근육의 원위부가 근위부 흑은 중심부에 비하여 체온이 낮고, 산소의 공급이 지연되어 근육의 손상이 적다는 보고가 있다 이에 저자들은 허혈후 재관휴시 폐색근위부와 원위 부의 근육에서 허혈후 재판류시 형성되는 반응성 산소기의 자극으로 발현되거나 활성화되는 SOD 활성의 변동을 관찰하였다, 실험동물로는 체중 200g-250g 사이의 건강한 Sprague-Dawley 계 수컷 흰쥐를 사용하였으며, urethane (3 mg/kg) 의 복강내 투여로 마취시킨 후 하복부를 절개하고 온엉덩동맥을 혈관집게로 2시간 혈휴를 차단하여 허혈상태를 유발시켰고, 2 시간 허혈시킨 직후, 1시간, 2시간, 6시간, 12 시간, 24시간 및 48시간 동안 재관류한 다음 근위부근육인 넙다리곧은근과 원위부 근육인 장딴지근의 중간부위를 적출하였다. 적출한 조직은 동경절연을 제작하여 anti-human Cu, Zn-SOD 항체와 antthuman Mn-SOD 항체를 1차 항체로 하고 ABC kit와 DAB kit를 이용하여 SOD의 효소황성을 연역세포학적 방법으로 관찰하여 다음과 같은 결과를 얻었다. l. 정상대조군에서 Cu, Zn-SOD활성은 넙다리곧은근의 곤욕세포보다 장딴지은의 근육세포에서 높게 나타났고, Mn-SOD 활성은 두 근육의 근육세포에서 유사하게 나타났다 2. 2시간 허혈로 나타나는 효소의 활성은 각 근육세포마다 다르게 나타났으나 큰 차이는 없었다. 3. 허혈후 재관류시 Cu, Zn-SOD활성은 넙다리곧은근의 근육세포에서 재관류 시간 경과에 따라 증가하여 6시간 경과시 가장 높았고 이후 강소하다가 24 시간 경과시 다시 증가한 후 감소하였으며, 장딴지근의 근육께포에서는 2시간 경과시 가장 높았고 24시간 경과시 증가하여 정상애조군의 수준이 되었으며 이후 변동이 없었다. 4. 허혈후 재관유시 Mn-SOD 활성은 넙다리곧은근의 근육세포에서 허혈후 재관류로 시간 경과에 따라 증가하여 6시간 경과시 가장 높았으며 이후 감소하였고, 장딴지근의 근육세포에서는 6시간 벚 12시간 경과후 가장 높았으며 이후 감소하였다. 5. 넙다리곧은근과 장딴지근에서 허혈후 재관류시 관창된 근육세포에서의 Cu, Zn 및 Mn -SOD 활성은 각효소별로 변동의 양상과 폭이 유사하였다. 이상의 결과를 종합하연 허혈후 재관류시 폐색근위부 벚 원위부 근육의 근육세포에서 SOD 의 변동양상과 변동폭은 유사한 것으로 사료된다.

일산화탄소 폭로후 고압산소 재폭로시 흰쥐 신장 및 심장의 SOD 활성 변동에 관한 연구

전영희,백두진,황세진,박준숙,정호삼 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.1

Carbon monoxide is generated from the incomplete combustion of carbon-containing materials. Carbon monoxide cause muscle necrosis leading to myoglobinuria and acute renal failure. The hyperbaric oxygen exposure is increased production of active free radicals. Free radicals which promoted peroxidation of unsaturated fatty acids may destroy essential cellular components and itself cytotoxic materials. Superoxide dismutase, one of the protective mechanisms against tissue damage, catalyzed the dismutation of superoxide anion to less toxic product. The author performs the present study to investigate on Mn-SOD and Cu, Zn-SOD immunoreactivities in kidney and heart carbon monoxide (CO) and hyperbaric oxygen (HBO) exposure. The animals were divided CO exposure group, air release after CO exposure group, HBO exposure group and, CO and HBO exposure group. The specimen were sectioned in 16㎛ with cryostate. The Mn-SOD and CuZn-SOD immunohistochemistry was performed in renal corpuscle, proximal and distal convoluted tubules of kidney and, in sarcoplasm and sarcolemma of heart muscle. The results obtained were as follows: 1. The immunoreactivity of Mn-SOD were increased in renal corpuscles and distal convoluted tubules, were similar to control group in proximal convoluted tubules. 2. The immunoreactivity of Cu, Zn-SOD were increased than that of control group in renal corpuscles and distal convoluted tubules, were same that of the control group in proximal convouted tubules. 3. The immunoreactivity of Mn-SOD were increased at all experimental group of sarcoplasms of heart muscle. 4. The immunoreactivity of Cu,Zn-SOD were increased than that of control group in sarcolemma of heart muscle. These results suggest that the immunoreactivity of SOD after CO or HBO exposure is greatly increased in kidney and heart.

일산화탄소 및 고압산소의 폭로가 흰쥐의 제2형 폐포세포와 사구체에 미치는 영향에 관한 연구

백두진,정호삼,이계훈,황세진,전영호,전영희 한양대학교 의과대학 1997 한양의대 학술지 Vol.17 No.2

The present study was performed to investigate the ultrastructural changes of the type Ⅱ pneumocyte in the lung and that of the glomerulus in the kidney after carbon monoxide(CO) or hyperbaric oxygen (HBO) exposure. The male Sprague-Dawley rats, weighing about 200gm, were used as experimental animals. The animals were divided into CO exposure group, air exposure after CO exposure group, HBO exposure group and HBO exposure after CO exposure group. After single CO and HBO exposure (3790ppm), the specimen of lung and kidney were obtained. According to routine method, EM preparations were made and observed with electron microscope. The results obtained were as follows ; 1. Slight disruption of the mitochondrial membrane was observed in alveolar type Ⅱ pneumocyte, after single CO exposure. 2. In all experimental groups except CO exposure group, decrease in number and atropy of rough endoplasmic reticulum, disruption of double membrane and crista of mitochondria and vacuolar degeneration of lamellar bodies in alveolar type Ⅱ pneumocytes were observed. 3. In all experimental groups, fusion of the pedicles of podocytes, and obliterations of the endothelial fenestrations were observed. These results suggested that CO and HBO exposure may induced the ultrastructural damages in the alveolar type Ⅱ pneumocyte and the glomerulus.

난소절제가 흰쥐 대동맥의 미세구조에 미치는 영향

최용귀,전영희,백태경,백두진,정호삼 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.2

Cisplatin에 의한 흰쥐 공장의 alkaline phosphatase 활성변화에 미치는 SOD의 영향

김성목,현성희,전영희,문원진,백두진 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

Cisplatin has been widely used as an effective anticancer platinum compound. Cisplatin incorporates with intrastrand or interstrand of the DNA and it inhibits the synthesis of the nucleic acid and protein. During the treatment of cancer patients, cisplatin acts on normally proliferating cells, which results in nephrotoxicity, nausea, vomiting, anorexia, bone marrow suppression and inner ear damage. These toxicities are related to oxygen free radicals formation by cisplatin, which inhibits the activity of the catalase and admages the mitochondria. The author has demonstrated the effect of superoxide dismutase(SOD), one of the antioxidant enzymes, on changes of alkaline phosphatase activities in the jejunum of the cisplatin treated rats. A total of 60 healthy Sprague-Dawley rats weighing about 200gm were used as an experimental animals. Cisplatin(6mg/kg) was administered intraperitoneally with pretreatment of 15000unit/kg of SOD or not. The experimental animals are sacrificed at 6 hours, 12 hours, 24 hours and 3 days after the administration of cisplatin. A part of jejunum were obtained and fixed in 10%-neutral formalin at 4℃. The speciments were sectioned 16㎛ thickness in frozen cryostat. The activity of alkaline phosphatase was evaluated histochemically by Gomori's method and incubation time of sections of specimens was 30minutes. The results were as follows. 1. SOD increase the activity of alkaline phosphatase in the epithelium of jejunum of the rat. 2. The activities of alkaline phosphatase of the epithelium of the mucosa decrease in jejunum of 6 hours and 12 hours of cisplatin treated rats. In the 24 hours and 3 days, activities of alkaline phosphatase of the epithelium increase. The atrophied villi are observed in the 12 hours and 24 hours of cisplatin treated group. 3. The activities of alkaline phosphatase increase in 12 hours and 3 days of cisplatin treated group with SOD pretreatment, the activities decrease in 6 hours of cisplatin treated group with SOD pretreatment. The activities of alkaline phosphatase in the 24 hours of cisplatin treated group with SOD pretreatment show a decided decrease over those of control group with SOD pretreatment. The atrophied villi are seen in the 12 hours and 24 hours of cisplatin treated group with SOD pretreatment. 4. The activities of alkaline phosphatase in the cisplatin treated group with SOD pretreatment are higher than those in the cisplatin treated group, except 24 hours of cisplatin treated group. 5. There are very little changes of alkaline phosphatase activities in the lamina propria, submucosa and muscle layer of all groups. Consequently, these results suggests that cisplatin decreases the activity of the alkaline phosphatase and SOD pretreatment decreases the range of the change in alkaline phosphatase activity of jejunum of the cisplatin treated rats.

cis-Dichlorodiammineplatinum(Ⅱ)이 발정주기에 따른 흰쥐 난관섬모 세포의 형태 및 섬모형성에 미치는 영향에 관한 전자현미경적 및 면역조직화학적 연구

김동옥,송양주,전영희,정호삼 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

electron microscope for morphological changes of cytoplasmic organelles and with light microscope for detection of tubulin substances in the cytoplasm during estrous cycle. Experimental animals(weighting 200gm. female albino rats) divided into 4 groups by the estrous cycles. Ampullar oviducts of these animals were excised at each estrous cycles. The specimen was made into immunocytological reaction slides for detection of tubulin in the cytoplasm, and the other specimen were made to ultrathin section for electron microscopy. All of specimens were examined with light and electron microscope. The results obtained are as follows. 1. The tubulin substances in ciliated cells of oviducts revealed strong reactions during proestrus and estrus but weak reactions during metestus and diestrus. 2. After cis-Platin treatment, tubulin substances in the cytoplasm of oviductal ciliated cells showed weak stain reactions but at diestrus, moderate reactions were seen similar to that of the control group. 3. The cilia and basal bodies of ciliated cells of ampulla oviducts after cisplatin treatment were decreased in number at all stages of estrous cycle. 4. Ciliated cells in cis-Platin treated rat revealed hypertropies of mitochondria, atrophies of Golgi complex, decrease of polyribosomes and segmented cisternae of rough endoplasmic reticulum at proestrus, estrus and metestrus. It is consequently suggested that cis-Platin would induce inhibition of ciliogenesis in oviductal ciliated cells during estrous cycles.

CS gas가 흰쥐의 호흡점막내 섬모세포에서 Tubulin 합성에 미치는 영향

이기득,전영희,백태경,백두진,정호삼 대한해부학회 1996 Anatomy & Cell Biology Vol.29 No.1

Cisplatin이 흰쥐 십이지장의 alkaline phosphatase 활성에 미치는 영향에 대한 연구

정낙은,권순호,전영희,현성희,백두진 한양대학교 의과대학 1995 한양의대 학술지 Vol.15 No.1

Cisplatin is a platinum compound, which has been widely used as an anticancer chemotherapeutic agent. During the treatment of cancer patients cisplatin acts on normally proliferating cells, which results in nephrotoxicity, nausea, vomiting, anorexia, bone marrow suppression and inner ear damage. Cisplatin inhibits the DNA synthesis, protein synthesis and cell division by incorporation to DNA strands. The author has investigated the effect of cisplatin on duodenum of rats histochemically observing the changes in the activity of alkaline phosphatase. The rats treated with 6mg per kg of cisplatin were sacrificed at 6 hours, 12 hours, 24 hours and 3 days after the drug administration. The rats of control group were administered only water for injection. The duodenum specimens were fixed in 10%-neutral formalin at 4 。and sectioned 16 。thickness in frozen cryostat. The activity of alkaline phosphatase was evaluated histochemically by Gomori's method and incubation time of sliced specimen was 30 minutes. The results were as follows. 1. In control rats, the activity of alkaline phosphatase were moderate positive in the epithelium of mucosa and weak or trace positive in lamina propria, submucosa and muscle layer. 2. In the epithelium of the mucosa the activity of alkaline phosphatase were weak positive in 6 hours of cisplatin treated rats, moderate or strong positive in 12 hours and 3 days of cisplatin treated rats and strong positive in 24 hours of cisplatin treated rats. 3. There are trace or weak reactivities in the lamina propria, submucosa and muscle layer of duodenum of all groups. Consequently, it is suggested that cisplatin reduces the activity of alkaline phosphatase at 6 hours after administration and the activities of alkaline phosphatase by regeneration and differentiation of epithelium in the duodenum of cisplatin treated rats.