Field Trial on Control Effect of Poison Baits against German Cockroaches

Ree, Han-Il,Lee, Du-Ho,Lee, In-Yong,Jeon, Soung-Hoo INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

최근 널리 사용되고 있는 몇가지 독먹이 제제의 바퀴 방제효과를 평가하기 위하여 서울시내 모대학의 실험동물 사육실과 한 고층아파트 건물을 대상으로 소규모 야외시험을 실시하였다. 실험동물 사육실에는 hydramethylnon 2% 제제 설치시 2주 후 96.6%, 4주 후 99.2%의 방제율을 보였고 이와같은 방제효과가 조사기간인 18주(97.8%) 까지 계속되었다. Chlorpyrifos 0.6% 제제의 경우는 설치 2주 후 93.5%의 방제율을 보였고 12주까지 동일한 수준으로 계속된 후 18주에는 69.9%로 감소하였다. 고층아파트의 경우, hydramethylnon 2% 제제 처리시 2, 4, 6 및 8주만에 각각 81.4%, 88.8%, 91.5% 및 95.6%의 방제율을 보였고, chlorpyrifos 0.6%의 경우, 처리 2, 4, 8주에 각각 61.3%, 66.6%, 72.0% 및 84.6%의 방제율을 보였다. Fenitrothion MC 5%의 경우, 2, 4, 6, 8주에 각각 53.2%, 52.5%, 58.5% 및 27.7%의 방제율을 나타냈다. 이와같이 각 제제의 방제율이 큰 차이를 보인 원인은 여러가지가 있을 수 있겠으나, 그중 한 요인으로는 설치한 약제의 양을 들 수 있을 것이다. 즉 1 box에 들어있는 독먹이의 양을 비교한 결과 hydramethylnon 66g(8.25g×8discs), Chlorpyrifos 29g(2.9g×10discs)였고 fenitrothion MC는 19.2g(3.2g×6discs)이었다. Small scale field trials on control effect of three poison bait products against German cockroaches(Blattella germanica) were carried out in laboratory animal rooms and an apartment building in Seoul. The trial in the laboratory animal rooms showed that hydramethylnon 2% bait treatment gave the reduction rate of 96.6%, 99.2%, 99.0%, 99.5% and 97.8%, in 2, 4, 8, 12 and 18 weeks after treatment respectively, and chlorpyrifos 0.6% bait treatment gave the reduction rate of 93.5%, 93.5%, 92.7%, 92.3% and 69.9% in 2, 4, 8, 12 and 18 weeks after treatment, respectively. The trial in the apartments showed that hydra-methylnon 2% bait treatment gave 81.4%, 88.8%, 91.5% and 95.6% of reduction rate on 2, 4, 6 and 8 weeks post-treatment respectively, chlorpyrifos 0.6% bait treatment gave 61.3%, 66.6%, 72.0% and 84.6% on 2, 4, 6 and 8 weeks post-treatment respectively, and fenitrothion MC 5% bait treatment gave 53.2%, 52.5%, 58.5% and 27.7% on 2, 4, 6 and 8 weeks post-treatment. Such differences in the reduction rate would be resulted from many factors, and the amount of the bait would be one of them, as shown that a package of the fenitrothion MC 5% bait and the chlorpyrifos 0.6% bait contained only 19.2g(3.2g×6discs) and 29g(2.9g×10discs) respectively whereas a package of the hydramethylnon 2% bait contained 66g(8.25g×8discs).

우리나라에 서식하고 있는 모기 ( Culex pipiens )의 항원 추출과 항원성 분석

이기영,이기선(Ki Sun Lee),이수영(Soo Young Lee),정병주,김규언,류정우(Jeong Woo Ryu),염혜영(Hae Yung Yun),이한일(Han Il Lee),용태순(Tai Soon Yong),전성후(Soung Hoo Jeon) 대한소아알레르기호흡기학회(구 대한소아알레르기 및 호흡기학회) 1999 소아알레르기 및 호흡기학회지 Vol.9 No.3

목 적 : 모기에 물린 후 나타나는 여러 가지 반응들은 혈청내 모기 특이 IgE 항체와 관련이 있는 것으로 알려져 있다. 이미 서구에서는 모기에 물린 후 팽진, 구진 등의 피부 국소반응과 출혈성 피부 병변, 혈관 부종, 천식 및 쇼크에 이르는 전신반응에 대한 증례 보고와 면역학적 연구가 시행되어 왔으나, 국내에서는 이에 관한 연구가 전무한 실정이다. 모기 항원은 모기의 몸체, 두흉부, 침샘, 침 등을 이용한 immunoblot 분석을 통해 알 수 있으며, 모기의 종에 따라 그 항원성은 상이하다. 정제된 항원의 제조는 모기 알레르기 환자의 진단 및 면역치료에 필수적이나, 상품으로 시판되는 모기 항원들은 현재까지 그 항원 농도와 단백질 농도가 일정치 않아 표준화가 필요한 것으로 판명되었다 이에 우리나라에서 서식하는 Culex pipiens의 항원을 추출하여 그 항원성을 분석해 보았다. 방 법 : 연세의대 소아과, 아주의대 소아과 알레르기 클리닉에 내원하여 시행한 피부 반응검사상 모기 항원에 양성 반응을 보인 환아들을 대상으로 하였다. 국립보건원 의동물학과로부터 사육중인 Culex pipiens를 공급받아 마취하에 두흉부를 분리하여 homogenizer를 이용하여 수용성 균질액을 만들고, 원심분리하여 filter에 투과시켜 조항원을 만들었다. Bradford method에 따라 단백질 농도를 측정하고, SDS-PAGE를 시행하여 분리한 후, nitroucellulose membrane에 전이시킨 후, 모기 항원으로 시행한 피부반응검사상 양성 반응을 보인 환아의 혈청을 이용하여 immunoblot을 실시하여 IgE와 결합하는 단백띠를 관찰하였다. 결 과 : 1) Culex pipiens 조항원의 단백질 농도는 10.88 mg/ml 이었다. 2) Culex pipiens 조항원의 SDS-PAGE 결과 20여개의 단백띠가 분리되었다. 3) Immunoblot 결과 70 kD, 60 kD, 34 kB의 단백띠가 IgE와 결합하였다. 결 론 ; 우리 나라에서 서식하는 모기 중 Culex pipiens의 조항원을 제조하였으며, 70 kD, 60 kD, 34 kD의 단백띠가 알레르기를 유발하는 항원으로 추측되며, 향후, 침샘 및 침을 이용한 정제된 항원을 이용한 연구가 시행되어야 할 것으로 기대된다. Purpose : Adverse reactions to mosquito bites have been recognized for some time, and these usually consist of large swellings, generalized urticaria, angioedema, and less frequently asthma. There have been some efforts to define immunologic characteristics of mosquito allergens throughout the world. But, in Korea, the immunologic analysis of mosquito allergen have not been made. Methods : Extracts of locally distributed female mosquito Culex pipiens, were prepared from their heads and thoraxes. By sodium dodecylsulfate-polyacrylamide gel electrophoresis, proteins of two extracts were isolated. Then their proein bands were transferred to nitrocellulose membrane. Immunoblotting was performed with sera of allergic patients to mosquito bites, then completed by antibodies to human IgE. Results : Immunoblotting showed IgE binding proteins weighing 70 kD, 60 kD, 34 kD in Culex pipiens. Conclusion : We found that mosquito antigens induced IgE response, and it suggested that species-specific antigens exist. Further investigation using salivary gland extracts from Culex genus are needed in identifying specific allergens.

Transforming variant of Met receptor confers serum independence and anti-apoptotic property and could be involved in the mouse thymic lymphomagenesis

백철민,전성후,장자준,이복순,이재호 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.4

Met tyrosine kinase receptor, the receptor of hepatocyte growth factor/scatter factor (HGF/SF), is present in mouse tissues as two major isoforms differing by a 47-aminoacid segment in the juxtamembrane domain via alternative splicing of exon 14. We found that the smaller isoform of Met (Sm-Met) was highly transformable in both in vitro and in vivo tumorigenesis assays. In this report, close examination of the transforming activity of the Sm-Met showed that the expression of Sm-Met conferred the cells serum independence and antiapoptotic property when treated with doxorubicin. These properties of Sm-Met seemed to be originated from its far longer maintenance of tyrosine kinase activity after the binding of HGF/SF. Interestingly, the longer maintenance of activated status was accompanied with more increase of tyrosine phosphorylation of Stat3 protein. Moreover, we have tried to find (an) animal tumorigenesis model(s) showing the increase in the expression of this transforming variant of Met. In γ- ray-induced mouse thymic lymphoma model, the expression of the mRNAs for Sm-Met was significantly increased as well as those of wild type Met and HGF/SF, suggesting a possible role of the Sm-Met in tumorigenesis in vivo.

Staphylococcus epidermidis로부터 새로운 단백질 분해효소 유전자의 cloning과 sequencing I: Subcloning for sequencing

하상덕,전성후,이현환 한국외국어대학교 외국학종합연구센터 부설 기초과학연구소 1996 기초과학연구 Vol.5 No.-

사람의 피부로부터 분리된 Staphylococcus epidermidis 균으로부터 새로운 단백질 분해효소 유전자들을 Cloning하고 동정하였다. 이들 protease 유전자들은 각각 0.7Kb와 1.2Kb의 크기였으며, 각각 pSS1과 pSS2로 명명하고 이들을 가진 재조합 plasmid를 대장균에 도입하였을 때 colony 주위에 투명한 환을 보였다. 이들 유전자들은 이미 보고된(J. Basic Sci., HUFS, 4, 1996, pp.211-221)것들과 크기나 유전자 지도가 달랐다. 이들 유전자들을 sequencing하기 위하여 몇 개의 subclone을 만들고 이들로부터 염기서열을 부분 결정하였으며, 정확한 유전자 지도를 작성하였다. Novel protease genes from Staphylococcus epidermidis, which was isolated from Human skin, were cloned and characterized. The insert size of these protease gene is 0.7Kb and 1.2Kb, respectively. The E. coli cell which harboring these plasmids showed a clear halo around the colony. The size and restriction map were different from those of previously reported pMG301, pMG302 and pMG303(J. Basic. Sci. HUFS. 4. 1996. pp.211-221). To determine the nucleotide sequence of the protease gene from pSS1, several subclones were made and characterized. Using the subclones, partial nucleotide sequence and fine restriction map were determined. The searching for complete ORF is now understudied.

데이터마이닝 기법을 이용한 소득 수준별 가계부채총액 예측모형 구축

김재진,전성아,황은혜,최정균,최종후 한국자료분석학회 2015 Journal of the Korean Data Analysis Society Vol.17 No.6

SFLC (the survey of household finances and living conditions) is a panel survey performed by Statistics Korea in collaboration with financial supervisory service, and the Bank of Korea, in annual basis based on same pool of households. The survey collects micro-information of income, debt, and expenditure by households characteristics, economic living condition, and duration of changes, where it is a unique survey providing information of household level in Korea. We investigate into construct classification and prediction model of amount of household debt based on 2014 SFLC,. The household debt can be classified into debt with investment purpose by households with high-level of income and sufficient assets, and household debt supplementing lack of living cost, and business fund by households with low-level of income, and lesser amount assets. We try to compare with the whole sample and highest 20 percent and lowest 20 percent empirically. Decision tree (CHAID and CART), and regression models are applied for classification and prediction model of amount of household debt. ‘2014 가계금융ㆍ복지조사’는 통계청이 금융감독원 및 한국은행과 공동으로 매년 동일한 가구를 조사하는 패널조사로 가구특성별 소득, 자산, 부채, 지출 등 가구의 재무건전성을 파악하고, 경제적 삶의 수준, 변화 지속기간 등을 미시적으로 파악하는 조사로서 가구 단위의 부채를 파악할 수 있는 유일한 조사이다. 본 연구는 ‘2014 가계금융ㆍ복지조사’에 기반하여 가계부채총액에 대한 분류(classification) 및 예측(prediction) 모형을 구축하고자 하는데 연구 목적을 두고 있다. 가계부채의 유형은 소득수준도 높고 자산도 풍족한 가계가 투자를 목적으로 하는 과정에서 늘어난 가계부채와 소득수준도 낮고 자산도 적은 가계가 부족한 생계비나 사업자금으로 인해 늘어난 가계부채로 나누어볼 수 있다. 본 연구에서는 이를 실증적으로 규명해 보고자 소득수준 최상위 20%, 최하위 20% 계층에 대하여 각각 별도의 모형을 구축하고 전체의 결과와 비교한다. 모형구축을 위하여 의사결정나무(decision tree)와 회귀(regression) 모형이 사용되는데 의사결정나무모형의 경우 CHAID와 CART 분리 알고리즘이 각각 적용된다. 구축된 경쟁 모형을 대상으로 모형 선호 기준통계량에 기반하여 가계부채총액의 분류 및 예측을 위한 추천 모형을 탐지한다.

p21Cip/WAF1 activation is an important factor for the ERKpathway dependent anti-proliferation of colorectal cancer cells

박기숙,전성후,오종원,최강열 생화학분자생물학회 2004 Experimental and molecular medicine Vol.36 No.6

p21Cip/WAF1, an important regulator of cell proli-feration, is induced by both p53- and extra-celular signal regulated kinase (ERK) pathways. The induction of p21Cip/WAF1 occurs by prolonged activation of the ERKs caused by extracellular stimuli, such as zinc. However, not all the cells appeared to respond to ERK pathway dependent p21Cip/WAF1 induction. Here we investigated the cause of such diference using colorectal cancer cells. p21Cip/WAF1 induction and concomitant reduc-tion of bromodeoxyuridine (BrdU) incorporation DLD-1. However, HCT-16 cels with high end-ogenous p21Cip/WAF1 levels did not show any ad-ditional increment of p21Cip/WAF1 levels by zinc treatment and did maintain high BrdU incor-poration level. The p21Cip/WAF1 induction by zinc depended upon prolonged activation of extra-cellular signal regulated kinase (ERK) was not ob-served in HCT-116 cells. The percentage of BrdU positive cells was 50% higher in p21Cip/WAF1 -/- HCT-116 cells compared to p21Cip/WAF1 +/+ HCT- 16 cels, and no cels induced p21Cip/WAF1 in-corporated BrdU in its nucleus, yet confirming the importance of p21Cip/WAF1 induction in anti- proliferation. These results again suport that p21Cip/WAF1 induction is a determinant in the re-gulation of colonic proliferation by the ERK pathway.

Fauna of Arthropods of Medical Importance in Chindo Island, Korea

Ree, Han-Il,Lee, Won-Ja,Lee, In-Yong,Jeon, Seoung-Hoo INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

1994년 7월 전라남도 진도에서 위생절지동물상을 조사하였다. 모기는 8종이 채집되었는데 그중 일본뇌염 매개종인 작은 빨간집모기(Culex tritaeniorhynchus)가 우점종이었다. 신종으로 보이는 Culex sp. 유충이 채집되었는데 명명은 성충이 채집되지 않아 보류하였다. 깔따구는 모두 11속 23종이 채집되었는데 그 중 Cladopelma viridula(녹색사촌무깔따구, 신칭), Dicrotendipes septemmaculatus(여섯점갈래깔따구, 신칭) 및 Harnischia urtilamellata(흑무깔따구. 신칭)는 한국 미기록종이어서 자세히 재기재하였다. 깔따구류중 우점종은 안개무늬날개깔따구(Chironomus kiiensis)이었고(67.3%). 노란깔따구Ch. flaviplumus)가 그 다음이었다(15.6%). 등에모기는 모두 5종이 채집되었고 그중 Culicoides punctatus가 88.7%로 우점종이었다. Foreipomia sp.도 채집되었는데 이 속은 한국에서 처음으로 기록되는 것이다. 들쥐 채집결과는 등줄쥐(Apodemus agrarius)가 높은 밀도를 보였고 등줄쥐에 기생하는 외부기생 절지동물로 Leptotrombidium orientale, Ixodes nipponensis, Laelaps jettmani, Eulaelaps stabularis등이 동정되었다. Fauna of medically important arthropods in Chindo Island was studied in July 1994. Among eight species of mosquitoes (Diptera: Culicidae), Culex tritaeniorhynchus was predominant (46.5% of the total), and Anopheles sinensis the next. Seven specimens of the unidentified Culex sp. which seemed to be new were also collected. Total 23 species of non-biting midges (Diptera: Chironomidae) were found in Chindo Is. Of them, Chironomus kiiensis was the predominant species (67.3%), and Ch. flaviplumus the next (15.6%). Three species, Cladopelma viridula, Dicrotendipes septemmaculatus and Harnischia urtilamellata are reported for the first time in Korea, and they are fully re-described with illustrations. Among five species of biting midges (Diptera: Ceraopogenidae) collected, Culicoides punctatus was predominant (88.7%). A species of Foreipomia was also collected, and this genus is not recorded in Korean fauna. Only two species of domicilary cockroaches, Periplaneta japonica and P. americana were collected in small numbers and Blatella germanica wich is known as the most predominant species in Korea was not found. The predominant species of field rodents was Apodemus agrarius and their ectoparasites were Leptotrombidium orientale (Acarina: Trombiculidae), Ixodes nipponensis (Acarina: Ixodidae), Laelaps jettmani and Eulaelaps stabularis (Acarina: Laelapidae).

Identification of salivary proteins in Aedes togoi (Theobald) that bind to mouse immunoglobulin E

Kyung‐Min Choi,이경열,전성후 한국곤충학회 2018 Entomological Research Vol.48 No.3

Mosquito saliva contains a number of immunogenic molecules that can induce allergic reactions in host animals. The oriental tiger mosquito, Aedes togoi (Theobald), feeds on both humans and animals and is also a vector of various pathogenic viruses. However, information on immunogenic antigens in the saliva of this mosquito species is scarce. In this study, we used immunohistochemical staining and confocal microscopy to determine the fine structure of the acini lobes of salivary glands of A. togoi. In addition, we identified proteins from salivary gland extracts that reacted to mouse immunoglobulin E in an immunoblot analysis. At least nine proteins, ranging in size from 10 to 145 kDa, bound to IgEs; a 33 and 57.5 kDa protein showed the strongest binding signal. Our results provide important information on the identities of potential allergens in mosquito saliva.

Comparative epidemiological studies on vector/reservoir animals of tsutsugarmushi deseasse between high and low endemic areas in Korea

REE, Han-Il,CHO, Min-Kee,LEE, In-Yong,JEON, Soung-Hoo INSTITUTE OF TROPICAL MEDICINE YONSEI UNIVERSITY 1995 YONSEI REPORTS ON TROPICAL MEDICINE Vol.26 No.1

쯔쯔가무시병 환자의 유병률이 가장 높은 남해안 연안지역과 유병률이 낮은 동해안 연안 지역에서 매개 진드기 및 들쥐와 연관되는 제반 역학적 비교조사를 1993년 10월에 실시하였다. 채집된 6종의 들쥐(187개체) 중 등줄쥐(Apodemus agrarius)가 양지역에서 모두 절대 우점종이었고 개체군 밀도에 있어서도 차이가 없었다(트랩 채집률 각각 20.4%, 19.4%). 국내 매개종으로 확인된 Leptotrombidium pallidum과 L. scutellare는 모두 채집된 6종의 들쥐중 등줄쥐에 주로 기생하였는데, 두당 각각 59마리와 69마리가 기생하고 있었다. 등줄쥐의 Rickettsia tsutsugamushi 병원체에 대한 항체 양성률은 남해안 43.2%, 동해안 63.6%로 동해안 지역에서 약간 높게 나타나 환자 발생과는 다른 양상을 보였다. 지역에 관계없이 등줄쥐의 감염률(항체 양성률)과 매개종인 L. scutellare와 L. pallidum의 개체군 밀도간에 높은 상관관계를 보였다(r=0.87). 이와같은 사실은 들쥐에 대한 양종의 매개 효율성(vector efficacy)이 똑같이 높다는 것을 의미한다. 양지역간의 가장 중요한 역학적 차이는 매개종으로, 남해안 지역에서는 L. scutellare가 절대 우점종(74.9%)인 반면에 동해안 지역에서는 L. pallidum이 절대 우점종 (60.4%)이었다. 두종의 개체군 밀도에는 큰 차이가 없었다. 조사지역이 불충분하여 확실하게 결론지을 수는 없지만, L.scutellare의 개체군 밀도가 높을수록 환자 유병률도 높게 나타나, L. scutellare가 인체에 대한 매개효율성(vector efficacy)이 L. pallidum보다 월등하게 높은 중요한 매개종이라 생각된다. Comparative epidemiological studies on vector reservoir animals of tsutsugamushi disease were carried out in between south coast (the highest endemic) areas and east coast (low endemic) areas in October 1993. Fauna of field rodents and their population densities were not different between two areas. Antibody positive rate of Apodemus agrarius sera was higher in east coast (43.2% in south coast and 63.6% in east coast). High correlation (r=0.87) was shown between antibody positive rate of A. agrarius sera and population density of the vector mites (Leptotrombidium scutellare and L. pallidum). L. scutellare was predominant in south coast, showing 110.6 chigger index (74.9% of the total chiggers), whereas L. pallidum was predominant in east coast, showing 126.3 chigger index (60.4% of the total). As higher population density of L. scutellare was found in south coast where the prevalence rate of tsutsugamushi disease is the highest, it result from more frequent vector-human contact.

A prospective, multicenter study on the clinical effectiveness of abiraterone in metastatic castration-resistant prostate cancer in Korea: Pre- vs. post-chemotherapy

정승환,연상은,김수연,권태균,전성수,최영득,권동득,정병하,홍성후,김병훈,이효진,신상준,최우석,박성우,강택원,윤석중,조진선,최세민,이나리,곽철 대한비뇨의학회 2023 Investigative and Clinical Urology Vol.64 No.5

Purpose: The proper treatment sequence for administering abiraterone acetate plus prednisolone (AAP) and chemotherapeutic agents has not yet been elucidated for metastatic castration-resistant prostate cancer (mCRPC). Hence, this study evaluated the effectiveness and safety of AAP in pre- and post-chemotherapy settings using real-world data. Materials and Methods: This prospective, multicenter, open-label, observational study included 506 patients with mCRPC. Patients were classified according to the timing of chemotherapy into pre- and post-chemotherapy groups. The effectiveness and safety of AAP were compared between the groups; the prostate-specific antigen (PSA) response, PSA progression-free survival, and radiologic progression-free survival were assessed; and adverse drug reactions were recorded. Results: Among the included patients, 319 and 187 belonged to the pre- and post-chemotherapy groups, respectively. Risk classification was similar between the two groups. The PSA response was 61.8% in the pre-chemotherapy group and 39.0% in the post-chemotherapy group (p<0.001). The median time to PSA progression (5.00 vs. 2.93 mo, p=0.001) and radiologic progression-free survival (11.84 vs. 9.17 mo, p=0.002) were significantly longer in the pre-chemotherapy group. Chemotherapy status was associated with PSA (hazard ratio [HR] 1.39, 95% confidence interval [CI] 1.09–1.77) and radiologic progression (HR 1.66, 95% CI 1.18–2.33) during AAP treatment. Adverse drug reactions were reported at similar frequencies in both groups. Conclusions: In this postmarketing surveillance, AAP benefited patients with mCRPC, especially in settings before chemotherapy was administered, resulting in a high PSA response and longer PSA and radiologic progression-free survival with tolerable adverse drug reactions.