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농식품 환경 분야(PF) : PF-13 ; 두 지역에서 재배된 배 중 Fenitrothion과 Myclobutanil의 잔류 패턴
전상오 ( Sang Oh Jeon ),황정인 ( Jeong In Hwang ),전영환 ( Young Hwan Jeon ),이상협 ( Sang Hyup Lee ),김장억 ( Jang Eok Kim ) 한국환경농학회 2014 한국환경농학회 학술대회집 Vol.2014 No.-
It is important to investigate biological half-lives (t1/2) of pesticides for predicting their residual amounts at harvest time. In this study, dissipation patterns of fenitrothion and myclobutanil were studied in two pear orchards, Yeongcheon (YC) and Gyungju (GJ), and pre-harvest residue limits (PHRLs) for the pesticides in pears were calculated using their half-lives. The residual amount of fenitrothion in YC pear at the harvest time slightly exceeded its maximum residue limits (MRLs) which is 0.20 mg/kg, whereas that in GJ pear was below MRLs. In case of myclobutanil, the residual amounts were below MRLs in pears of both regions. The dissipation constants of fenitrothion in YC and GJ pears were 0.055 (t1/2 = 12.6 days) and 0.040 (t1/2 = 17.3 days), respectively, and those of myclobutanil were 0.025 (t1/2 = 12.6 days) and 0.097 (t1/2 = 17.3 days), respectively. According to the calculated PHRLs, if the residual amounts of fenitrothion and myclobutanil in pears were 0.48~0.67 mg/kg and 1.20~ 1.69 mg/kg, respectively, at 10 days prior to the harvest, their residue amounts in pears at the harvest time would be below the MRLs.
04 포스터 발표 : 환경 화학 분야(PC) ; PC-04 : 미나리와 들깻잎 중 살균제 Boscalid의 잔류특성 비교
전상오 ( Sang Oh Jeon ),황정인 ( Jeong In Hwang ),이상협 ( Sang Hyeob Lee ),경기성 ( Kee Sung Kyung ),김태화 ( Tae Hwa Kim ),권찬혁 ( Chan Hyeok Kwon ),손영욱 ( Yeong Uk Son ),김동술 ( Dong Sool Kim ),김장억 ( Jang Eok Kim ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-
Different growth type of crops is one of major factors determining residual patterns of pesticides in them. In this study, residual characteristics of boscalid in water dropworts (WD) and perilla leaves (PL) which are different in leaf size were investigated. Commercial boscalid (49.3%, WP) was sprayed 2 or 3 times at interval of a week prior to harvest, and crop samples were collected at both final application and harvest days. Residual amounts of boscalid in the WD and PL were analyzed using GC-ECD, and recovery rates of boscalid spiked with 0.1 and 0.5 mg/kg on the WD and PL ranged from 91.8 to 111.0%. Initial residual amounts of boscalid were 20.3-44.5 mg/kg in WD and 101.6-138.7 mg/kg in PL. These results show that initial amounts of boscalid deposited on PL may be 4 folds larger than those in WD, when assuming that weights of the WD and PL are same. At the harvest day, residual amounts of boscalid in WD and PL decreased by 35.6% (13.0-28.9 mg/kg) and 21.1% (88.2-98.6 mg/kg), respectively, of the initial residual amounts. Pesticides applied on PL, which has wide leaves, may have larger residual amount and longer persistency than those on WD.
농식품 환경 분야(PF) : 국내 주요 유통 농산물 중 엽경채류에 대한 잔류농약의 통계학적 및 확률론적 분포에 따른 위해성평가
이상협 ( Sang Hyeob Lee ),전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),전상오 ( Sang Oh Jeon ),이성은 ( Sung Eun Lee ),장한섭 ( Han Sub Jang ),김장억 ( Jang Eok Kim ) 한국환경농학회 2014 한국환경농학회 학술대회집 Vol.2014 No.-
최근 3년간(2010년~2012년) 국립농산물품질관리원에서 실시한 엽경채류 중 주요 유통 농산물 10종(들깻잎, 미나리, 배추, 부추, 상추, 시금치, 쑥갓, 양배추, 얼갈이배추, 열무)에 대한 농약 잔류실태조사 결과, 조사된 12,849건 중 MRL을 초과한 시료는 427건으로 부적합 시료는 0.03% 이었다. 부적합 시료 중 MRL을 초과한 농약은 acrinathrin 외 73종이었다. 엽경채류 10종 중 acrinathrin외 73종에 대한 통계학적 위해성평가를 위하여 @Risk 통계프로그램을 이용한 극단(95 percentile) 및 초극단(99 percentile) 값을 바탕으로 연령별 %ADI 및 %ARfD 평가를 실시하였다. 또한 ADI와 ARfD를 초과할 수 있는 확률론적 위해성 평가는 R 프로그램을 사용하여 Monte-Carlo simulation을 통해 수행하였다. 그 결과, 연령별 %ADI 위해도는 소아기 0.0000~0.4416%, 청소년기 0.0000~0.1486%, 성인기 0.0000~0.4276%로 나타났으며 극단값은 소아기에 최고 0.2947%, 초극단값은 소아기에 최고 0.6595%로 나타났다. 또한, 연령별 %ARfD 위해도는 소아기 0.0000~0.0074%, 청소년기 0.0000~0.0049%, 성인기 0.0001~0.0447%로 나타났으며 극단값은 소아기에 최고 0.0022%, 초극단값은 소아기에 최고 0.0961%로 나타났다. 확률론적 위해성 평가를 통해 엽경채류 중 73종 농약의 ADI 및 ARfD 초과 확률을 산출한 결과, ADI를 초과할 확률은 0.00~0.28%, ARfD를 초과할 확률은 0.05%로 극히 낮은 수준이었다. 따라서 다양한 통계학적 기법을 이용한 위해성평가 결과들로 미루어 보면 엽경채류 중 잔류농약 노출에 의한 위해성은 모두 낮은 것으로 나타났다.
04 포스터 발표 : 농식품 환경분야(PF) ; PF-03 : 엽채소 작물 중 잔류농약 모니터링 결과에 대한 통계학적 해석
이상협 ( Sang Hyeop Lee ),황정인 ( Jeong In Hwang ),전상오 ( Sang Oh Jeon ),강민수 ( Min Su Kang ),정혜현 ( Hye Hyun Jeong ),장한섭 ( Han Sub Jang ),김장억 ( Jang Eok Kim ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-
A risk assessment method using @Risk program was developed for evaluating a number of analysis data on residual pesticides in leafy vegetables produced in Korea. Results of pesticide residue analysis conducted by National Agricultural Products Quality Management Service (NAQS) between 2010-2012 were used as the residual data for the risk assessment. The average of unacceptable rate for residual pesticide in leafy vegetables was 0.03% which is equivalent to 427 out of the total 12,849 times. It is only 0 to 1% of ADI value for the analyzed pesticides, indicating that the tested leafy vegetables were safe as they were below the their MRLs. The exposure assessment was done using @Risk program to find 95% value and 99% value. Results of the statistical analysis showed that the residual pesticides in leafy vegetables were determined to be below the MRL value, and that it was safe to the consumers of infants, adolescences, and adults. Using the distribution of diet consumption on leafy vegetables and the residual pesticides, the Monte-Carlo simulation showed ADI and ARfD values for the residual pesticides in leafy vegetables ranged from 0.00 to 0.28%. Therefore, it was not exceeding over ADI and ARfD.
03 구두발표 : 구두발표(OA) - 농업환경 및 토양,수질 분야 ; OA-02 : 토성이 다른 토양 중 Endosulfan 이성질체의 잔류양상 예측
황정인 ( Jeong In Hwang ),전상오 ( Sang Oh Jeon ),이상협 ( Sang Hyeob Lee ),강민수 ( Min Su Kang ),정혜현 ( Hye Hyun Jung ),곽세연 ( Se Yeon Kwak ),이성은 ( Sung Eun Lee ),김장억 ( Jang Eok Kim ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-
Pesticides persisted in soil environment may be subjected to the unexpected uptake by plant root. As these absorbed pesticides can result eventually in safety problems to agricultural products, it is required to predict the plant uptake extent of soil-persisting pesticides inadvance. One of the previous prediction methods is to assess the uptake extent using a mathematical model. When developing the plant uptake model of soil-persisting pesticides, adsorption and dissipation interactions between pesticides and soil may be parameters that play major roles in determining the uptake extent into plant. With considerations of parameters relative to these interactions, we developed a chemical-specific residue (CSR) model, Ce(t)=C0·(1/2)t/T/Kd, in order to predict the residual patterns of endosulfan isomers ( - and -) in soils containing different organic matter contents. The adsorption (Kd) and dissipation (T) parameters were obtained from indoor experiments, and the applicability of CSR model was assessed by comparing between experimental measurements and modeled estimates. The Kd and T values were significantly varied depending on organic matter contents in the tested soils. For the low organic matter-containing soil, the Kd and T values of the -isomer were 50.2 mL g-1 and 16 d, respectively; the values of the -isomer were 54.4 mL g-1 and 231 d, respectively. On the other hand, for the high organic matter-containing soil, the Kd values were 377.4 mL g-1 for the -isomer and 407.4 mL g-1 for the -isomer, and the T values for the - and -isomers were 139 and 347 d, respectively. Using these parameter values, the modeled values were in good agreement with the measured values with standard errors of less than 23.5%. Therefore, the developed CSR model may be used as a tool for predicting the residual amount of endosulfan in soil.
식품의약품안전처 SESSION : 고감도 신속 다종농약 다성분 검색/검사법 개발 연구-LC-MS/MS법
전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),김장억 ( Jang Eok Kim ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-
현재 세계적으로 자유무역협정(FTA: Free Trade Agreement)으로 인하여 수입식품의 양과 종류 도 매년 지속적으로 증가하여 시중 어디서든지 손쉽게 수입농식품을 구할 수 있다. 수입농산물의 양이 점점 늘어감에 따라 수입농산물의 안전성 평가가 주요한 현안으로 등장하고 있다. 따라서 식 품 안전에 대한 국내외적 경쟁력 확보를 위하여 잔류농약관리의 PLS 체계 도입 필요하다. 본 연구 는 LC-MS/MS를 이용하여 PLS 체계용 고감도 신속 다성분 검색법 개발하고자 한다. 국내에 등록 된 91종 농약의 다성분 동시분석법을 개발하기 위하여 식품공전상의 "다종농약다성분 동시분석법-제2법"에 적용하였다. 현미, 오렌지와 고추시료를 acetonitrile 로 추출한 후, 이를 감압여 과 한 후 여액에 sodium chloride을 넣어주었다. 여과액을 흔들어 섞어 주고 정치하여 acetonitrile층과 물층을 분리시켰다. 상등액인 acetonitrile층 취하여 감압 농축한 후 잔류물을 재용해하여 이를 정제 하였다. SPE cartridge를 이용하여 정제한 후 재용해하여 LC-MS/MS로 분석하였다. 국내미등록 농약 91종 대한 잔류분석법의 벨리데이션을 실시한 결과 대상 분석성분 부근에 시료 중 불순물에 의한 간섭은 관찰되지 않았으며, 검량선의 matrix-matched법으로 사용하였다. 본 연구 에서 확립된 시료조제 및 기기분석과정을 수행하여 얻은 회수율을 산출한 결과는 55∼162%(n=6) 로 나타났으며, 분석오차는 25% 이내로 나타났고, 정량한계는 0.01 mg/kg 이하였다. 본 시험에 의해 확립된 91종에 대하여서는 간단한 모니터링으로는 사용이 가능할 것으로 생각되 며, 앞으로도 나머지 농약에 대하여서 PLS체계를 위한 다성분 분석법 대한 연구가 지속적으로 수행되어야 할 것으로 생각된다.
농산물에 대한 LC-MS/MS를 이용한 농약의 다성분분석법 확립
전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),도정아 ( Jung Ah Do ),오재호 ( Jae Ho Oh ),홍진환 ( Jin Hwan Hong ),이영득 ( You 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-
Pest control is major problem in front of farmer worldwide, an enormous economic loss is observed due to pest every year. There are several chemical pesticides available for controlling agricultural pests. Studies have shown chemical pesticides can cause significant health risks to humans, contaminate water supplies, and to plant itself. To come through this problem, we previously developed some natural pesticides i.e. oak pyroligneous liquor, rice bran and phytic acid which were tested against Perilla frutescens rust disease. We found good pesticide capacity of these natural extracts, so we were veracious to know whether it has toxic effect or not for that we designed the present investigation. In the present study we have determined toxicity on the basis of fish survival assay, seed germination assay and mice feed assay. Briefly, a concentration of 50 and 100μg\ml of all pesticides were supplied in water of fishes for 96hrs. We have taken two kind of fishes for this experiment Misgurnus anguillicaudatus(10) and Cyprinus carpio(10). After completion of the incubation period number of dead fish were calculated. Simultaneously, we determined seed germination test on four types of seed Cabbage, Radish, Black beans and Red kidney beans. A number of 20 seeds in case of red kidney beans 5 seeds were kept in sterile Petri dishes bedded with layer of tissue papers (Kimtec) which were wet with autoclaved distilled water. Treatment of pesticides was done with a concentration of 50 and 100μg\ml for 48 to 96 hrs. The number of non-germinated seeds was counted after the incubation period. In the case of animal toxicity test we supplied pesticides in the drinking water of mice and they were kept under investigation of body weight and death of mice. The results obtained from fish experiment suggest no toxicity of oak and rice bran but at a higher concentration one fish was found dead. In the case of seed germination assay all the seeds were observed to be germinated at both concentrations for all pesticides. No decrease in body weight was found in mice experiment as well as all mice were live. In accordance with all data, we concluded that the use of Oak, Rice bran and Phytic acid as a pesticide is safe and effective especially in the case of Perilla rust disease.
Glutathion S-Transferase에 의한 Tolclofos-Methyl의 Biotransforation
김정민 ( Jung Min Kim ),전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),김장억 ( Jang Eok Kim ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-
Tolclofos-methyl(TM) is a fungicide of thiophosphate group which is widely used to control of soil born diseases and damping-off. However, its excessive usages are lead to the accumulation of this compound on soil and crops. The major TM metabolic pathways are oxidative desulfuration of TM to oxone and related derivatives, oxidation of the 4-methyl group to alcohols and acids, cleavage of P-O-aryl and P-O-methyl linkages and conjugation of the resultant acid with glycine. Glutathione S-transferases(GSTs) are the enzymes that detoxify natural and exogenous toxic compounds by conjugation with glutathione(GSH). The objective of this study was to know the fate of TM in crops through the GST dependant biotransformation for detoxification. First of all, We liked to present the possibility for conjugation between TM and GSH by GST in extracts of pig liver in vitro. We verified our result by specific GST activity assay using dinitrochlorobenzene and found that enzyme activity of pig liver extract is the highest among several sample such as crops. Recoveries for TM analysis at two fortification levels of 1.0 and 5.0 mg/kg were 93.1 ± 1.8% and 96.8 ± 4.7%, respectively. Removal ratio of TM were 15.6∼28.5% in buffer with extract of pig liver for 2day, at 30℃. The results suggested that the GST isolated from pig liver might have a role in the protection mechanism against toxic materials such as a TM.