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Nested PCR 기법을 이용한 인삼 뿌리썩음병원균의 특이적 검출
장창순,김홍기,송정영,이정주,김선익,유성준 한국식물병리학회 2005 식물병연구 Vol.11 No.1
Cylindrocarpon destructans is a soil-borne plant pathogenic fungus causing root rot on ginseng and trees. Rapid and exact detection of this pathogen was practiced on ginseng seedlings by nested PCR using speciesspecific primer set. The second round of PCR amplification by Dest 1 and Dest 4 primer set formed 400 bp of species-specific fragment of C. destructans from the product of first round of amplification by ITS 1 and ITS 4 primer set. In the PCR sensitivity test based on DNA density, nested PCR detected to the limit of one fg and it meant the nested PCR could detect up to a few spores of C. destructans. Also, nested PCR made it possible to detect the pathogen from ginseng seedlings infected by replantation on artificial infested soil. Our nested PCR results using species-specific primer set could be utilized for diagnosis of root rot disease in ginseng cultivation. Cylindrocarpon destructans는 인삼 및 수목에 뿌리썩음병을 일으키는 토양 전염성 식물병원균이다. 신속 정확한 검출 가능성을 알아보기 위하여 종 특이적인 primer와 nested PCR 기법을 활용하여 인삼 유묘로부터 뿌리썩음병균 C. destructans의 검출을 시도하였다. ITS 1과 ITS 4 primer를 이용한 1차 PCR 증폭산물에 대해 Dest 1과 Dest 4 primer로 2차 PCR 증폭을 실시한 결과 병원성이 확인된 C. destructans에서만 400 bp의 종특이적 증폭산물을 얻을 수 있었다. 종 특이적 primer와 nested PCR 기법을 이용한 인삼뿌리썩음병균 DNA에 대한 반응 민감도는 최저 약 1 fg으로 나타나 단 몇 개의 포자만 존재해도 검출이 가능하였다. 또한, nested PCR 기법은 실제 이병토양에 심었을 경우에도 C. destructans에 감염된 인삼 유묘로부터만 정확하게 병원균을 검출해 내었다. 종특이적 primer와 nested PCR 기법을 이용한 본 연구 결과는 실제 재배농가에서 인삼 경작시 뿌리썩음병 진단에 매우 유용하게 활용될 수 있을 것으로 판단된다.
홍기천,노경빈,이성웅,장창순,노준양,이두선 대한응급의학회 1993 대한응급의학회지 Vol.4 No.1
It is important work in the department of emergency medicine to manipulating data for the emergency patients. We developed a computer program for effectively managing data of the emergency patients and department on Dec. 1991, and began to build database on Jan. 1992. This program offers the various tools and functions for manipulating data, for quickly finding and displaying the information as you need, and for obtaining the basic results and analyses for quality assurance. The program was coded in a programming mode of FoxPro 2.5, and runs on IBM-PC-compatible computers. The systemic configurations to use properly, requires DOS 5.0 in O.S(operating system), 4 Megabytes or more in RAM(Random Access Memory), 100 Megabytes or more in Hard Disk capacity, at least 10 Megabytes in available disk space, and intel-80386(32 bits) in computer processing chip. The 8 bits combination codes are applied to write "Hangeul"(Korean language character). This program provides the various functions as the followings. 1. Be able to input, output, and search data as you need. 2. Be able to obtain the various reports by analysing the patient data. 3. Be able to retrieve the article data in Journal of Korean Society of Emergency Medicine, American Journal of Emergency Medicine, and Annals of Emergency Medicine as keywords, authors, etc. 4. Be able to manage the departmental data. By examples, manager of the emergency members, scheduler of the department, and collection book of the common senses in emergency medicine. 5. Other programs, which to reset and back up the selected data files for safely maintaining program, are supplied 6. Be able to study and prospectively analyze the clinical data by inserting the predefined optional programs. We expect the more advanced and beneficial program to manage data for the emergency patients and department.
국내 배추 뿌리혹병균, Plasmodiophora brassicae의 race와 그 우점 양상
장세정,임용표,김홍기,허승환,장창순,강성우 한국식물병리학회 2007 식물병연구 Vol.13 No.1
Single spores were isolated from infected roots of Chinese cabbage with a typical clubroot symptom, collected from different Chinese cabbage cultivation areas in Korea. When the single spore isolates were inoculated on Chinese cabbage, radish, turnip, kale, leaf mustard and Williams' differential varieties, among 321 roots harvested two weeks after inoculation, a visual symptom was observed on only one root and light/uncommon symptoms were done on 70 roots. These 71 individuals were homogenized and used as inocula. These inocula caused generally higher pathogenicity than that of single spore. Finally 15 isolates, with enough growth for conducting further experiment, were selected. These 15 individuals were grouped four, seven, two and two into race 1, race 4, race 9 and race 11, respectively, using Williams' differential set. It was confirmed that race 4 were dominantly present in Korea. These 15 had been obtained from roots of Chinese cabbages, radishes and turnips inoculated with single resting spores and had shown pathogenicity to Laurentian and Wilhelmsburger belong to Rutabaga in Williams' differential variety set. Therefore, we assume that such characteristic pathotypes including race 4, especially, of P. brassicae showing strong pathogenicity to Chinese cabbage, radish and turnip may be dominant in Korea.