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장지환,최권영 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Yarrowia lipolytica is well known for fatty acids and fatty alkanes biotransformation. it has 12 kinds of Alk monooxygenase enzymes and some are known to be responsible for fatty alkane ω-oxidation. Oxyfunctionalization of fatty alkanes, especially at ω-position, generates very interesting oxidative metabolites which could be converted further into various biopolymer monomers such as polyamide. One of the most important oxidation enzymes is alcohol dehydrogenase enzymes (ADHs) which could convert ω-hydroxyfatty acids into ω -oxofatty acids. However, not much information about Y. lipolytica ADHs were not elucidated. Thus, in this study, we cloned and expressed Y. lipolytica ADHs in E. coli. In order to solubilize the ADH enzymes, several optimization strategies like inducer concentrations, co-expression with molecular chaperones were investigated. The E. coli cells which expressed Y. lipolitica ADH could produce dodecanal up to 37.27 mg/L in whole cell biotransformation of dodecanol.
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장지환,최권영 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.2
In this study, alcohol dehydrogenases (ADH) enzymes from Yarrowia lipolytica were investigated for the cloning, soluble expression, and biotransformation of 1-dodecanol to 1-dodecanal, which reaction was thermodynamically unfavorable. Sole expression of ADHs in Escherichia coli did not produce soluble form of cytosolic protein, in spite of the effort to solubilize ADH protein by optimizing IPTG concentration, temperature, and auto-induction medium. Eventually, the active form of soluble ADH proteins was successfully obtained through the co-expression of ADH with chaperone protein in pG-KJE8 vector. After analyzing the individual sets of optimization, it was determined that pET-28a(+)::adh coexpression with the pG-KJE8 molecular chaperone in LB medium with 0.1 mM IPTG, 4 mg/mL arabinose, and 2 ng/mL tetracycline achieved optimum expressions against all of the five ADH proteins. Finally, the whole cell biotransformation activity of ADH2 was determined in 1-dodecanol oxidation to 1-dodecanal, followed by further oxidation to 1-dodecanoic acid.
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Predictors of 30-day Mortality and 90-day Functional Recovery after Primary Pontine Hemorrhage
장지환,송윤규,김영준 대한의학회 2011 Journal of Korean medical science Vol.26 No.1
The factors related to death and functional recovery after primary pontine hemorrhage (PPH) in Koreans has not been well defined. The authors sought to identify independent predictors of death and functional recovery after PPH using data obtained at a single institute. Data were collected retrospectively on 281 patients with PPH admitted to the Stroke Unit at our hospital between January 1, 2000 and December 31, 2009. Multivariate logistic regression analysis was used to evaluate the associations between selected variables and 30-day mortality and 90-day functional recovery after PPH. One-hundred and ten patients (39.1%) died within 30 days of PPH and 27 patients (9.6%) achieved functional recovery within 90 days. By multivariate analysis, unconsciousness, dilated pupils,abnormal respiration, systolic blood pressure < 100 mmHg, hydrocephalus, and conservative treatment were found to be predictors of 30-day mortality, whereas consciousness, intact motor function, no history of hypertension or diabetes mellitus,intact eye movement, a hematoma volume of < 5 mL, no ventricular hemorrhage, and normally sized ventricle were found to be predictors of 90-day functional recovery. The present findings suggest that systolic hypotension of less than 100 mmHg may predict 30-day mortality and a history of underlying hypertension and diabetes mellitus may predict 90-day functional recovery.
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장지환(Jihwan Jang) 원광대학교 동양학대학원 2016 동양학연구 Vol.12 No.-
우리가 현재의 생을 잘 사는 것도 필요하지만 죽음을 잘 맞이하는 것도 필요하다. 그것은 오늘날 웰빙이라는 용어와 마찬가지로 웰다잉이라는 용어가 유행하고 있는 것과 그 궤를 같이한다고 본다. 그렇다면 삶과 죽음을 잘 맞이하는 방법에 대하여 관심을 가질법한 일이다. 특히 종교의 역할 가운데 이러한 문제를 간과할 수 없으며, 종교의 중심역할이 이러한 의례로서 나타난다. 원불교 의례가운데 천도의례는 이러한 면에서 매우 주목되는 바이며, 이와 관련한 소태산 대종사의 가르침이 주목된다. Though we need to live well in the present, it is necessary for facing our death. It is around the track with Well-being and Well-dying at the present day. If it is so, we probably show some interest in the ways to welcome life and death. In particular, central rituals of religion can not passing over playing the role of taking that kind of reaction. Among Won Buddhist rituals, it is taking note of the way of delivering the Dead to the paradise as well as the Great Master Sotaesan’s teaching connected with that deliverance.
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