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장성필,조찬영,남진현,임시형,신동훈,정태용 대한기계학회 2010 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.24 No.4
Pressure signals measured in gas pipelines provide useful information for monitoring the status of pipeline network operations. This study numerically investigates leakage detection and location in a simple gas pipeline branch using transient signals from an array of pressure sensors. A pipeline network simulation model was developed and used to predict one-dimensional compressible flow in gas pipelines. Two monitoring methods were tested for leakage detection and location; these include cross-correlation monitoring and pressure differential monitoring. The performance and reliability of the two monitoring methods were numerically assessed based on simulation results for a simple pipeline branch operation with a prescribed leakage rate (5% of total flow rate in the gas pipeline).
Development of a strategy for the screening of α-glucosidase-producing microorganisms
Bo Zhou,Nan Huang,Wei Zeng,Hao Zhang,Guiguang Chen,Zhiqun Liang 한국미생물학회 2020 The journal of microbiology Vol.58 No.2
α-Glucosidase is a crucial enzyme for the production of isomaltooligosaccharide. In this study, a novel method comprising eosin Y (EY) and α-D-methylglucoside (AMG) in glass plates was tested for the primary screening of α-glucosidaseproducing strains. First, α-glucosidase-producing Aspergillus niger strains were selected on plates containing EY and AMG based on transparent zone formation resulting from the solubilization of EY by the hydrolyzed product. Conventional methods that use trypan blue (TB) and p-nitrophenyl-α-Dglucopyranoside (pPNP) as indicators were then compared with the new strategy. The results showed that EY-containing plates provide the advantages of low price and higher specificity for the screening of α-glucosidase-producing strains. We then evaluated the correlation between the hydrolytic activity of α-glucosidase and diffusion distance, and found that good linearity could be established within a 6–75 U/ml enzyme concentration range. Finally, the hydrolytic and transglycosylation activities of α-glucosidase obtained from the target isolates were determined by EY plate assay and 3,5- dinitrosalicylic acid-Saccharomyces cerevisiae assay, respectively. The results showed that the diameter of the transparent zone varied among isolates was positively correlated with α-glucosidase hydrolytic activity, while good linearity could also be established between α-glucosidase transglycosylation activity and non-fermentable reducing sugars content. With this strategy, 7 Aspergillus niger mutants with high yield of α-glucosidase from 200 obvious single colonies on the primary screen plate were obtained.
The Comparison of the 3D graph for the energy-equal of LEACH-Mobile
장성필,정계동,이종용 한국인터넷방송통신학회 2017 Journal of Advanced Smart Convergence Vol.6 No.1
In this paper, propose an algorithm to improve network lifetime by equally consuming energy of LEACH - Mobile sensor nodes. LEACH is one of energy efficient protocols. However, we did not consider the mobility of nodes. Therefore, the transmission reception success rate of the moving data is reduced. LEACH-Mobile is a protocol that has improved the drawbacks of these LEACH. However, since LEACH-Mobile has a larger number of data packets and consumes more energy than LEACH, it has a disadvantage that the lifetime of the network is short. In order to improvement these disadvantage, Based on the average of the remaining energy of the node, cluster heads are elected with a number of nodes whose energies are larger than the average of the remaining energy from the member nodes. After that, by trying to increase the lifetime of the network by equalizing the remaining energy. In to confirm whether improve the lifetime of the network, In this paper, the number of nodes and the position of all nodes are varied for each specific round, the rest energy is equalized, and the algorithm which uniformly selected the cluster head is compared with LEACH.
Demonstration of Alternative Fabrication Techniques forRobust MEMS Device
장성필,박재영,Doo Yeol Cha,Heung Shik Lee 한국전기전자재료학회 2006 Transactions on Electrical and Electronic Material Vol.7 No.4
This work describes efforts in the fabrication and testing of robust microelectromechanical systems (MEMS). Robustness is typically achieved by investigating non-silicon substrates and materials for MEMS fabrication. Some of the traditional MEMS fabrication techniques are applicable to robust MEMS, while other techniques are drawn from other technology areas, such as electronic packaging. The fabrication technologies appropriate for robust MEMS are illustrated through laminated polymer membrane based pressure sensor arrays. Each array uses a stainless steel substrate, a laminated polymer film as a suspended movable plate, and a fixed, surface micromachined back electrode of electroplated nickel. Over an applied pressure range from 0 to 34 kPa, the net capacitance change was approximately 0.14 pF. An important attribute of this design is that only the steel substrate and the pressure sensor inlet is exposed to the flow; i.e., the sensor is self-packaged.