In vitro에서 β-site amyloid precursor protein-cleaving enzyme 활성과 amyloid β protein 생산에 대한 총명탕가미방(聰明湯加味方)의 효과

임정화,정인철,임종순,김승형,이상룡,Lim, Jung-Hwa,Jung, In-Chul,Lim, Jong-Soon,Kim, Seung-Hyung,Lee, Sang-Ryong 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.2

Objectives : This experiment was designed to investigate the effect of Chongmyung-Tang Prescription Combination(CmTP-$C_{1-10}$) extract on the production of amyloid $\beta$ protein and $\beta$-site amyloid precursor protein-cleaving enzyme(BACE) activity. Methods : The effect of CmTP-$C_{1-10}$ extract on expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by lipopolysacchride(LPS) and amyloid $\beta$ protein fragment(A$\beta$ fragment) were investigated. The effect of CmTP-$C_{1-10}$ extract on production of amyloid $\beta$ protein(A$\beta$) in BV2 microglia cell line treated by LPS and A$\beta$ fragment were investigated. The effect of CmTP-$C_{1-10}$ extract on BACE activity were investigated. Results : 1. CmTP-$C_9$ extract the most significantly suppressed the expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by LPS and A$\beta$ fragment. 2. CmTP-$C_9$ extract significantly suppressed the production of A$\beta$ in BV2 microglia cell line treated by LPS and A$\beta$ fragment. 3. CmTP-$C_9$ extract the most significantly inhibited BACE activity. Conclusions : These results suggest that CmTP-$C_9$ may be effective for the prevention and treatment of Alzheimer's Disease. Investigation into clinical use of CmTP-$C_9$ for Alzheimer's Disease is suggested for future research.

신생아 집중치료실 입원아에 있어서 전신성 칸디다증의 임상적 특징 : 전신성 세균 감염증과 비교

임정화,박경필,김진경,김행미,Lim, Jung Hwa,Park, Kyung Pil,Kim, Jin Kyung,Kim, Heng Mi 대한소아청소년과학회 2002 Clinical and Experimental Pediatrics (CEP) Vol.45 No.7

목 적: 신생아 집중치료실에 장기간 입원 중인 환아는 입원중 전신성 감염을 일으킬 위험이 있다. 이미 항생제를 투여 받고 있는 환아에서 전신 감염 증상이 발현하면, 전신성 칸디다증은 장기간의 amphotericin B의 투여가 필요하며 세균 감염증은 감수성 있는 항생제로의 교체를 필요로 한다. 본 연구에서는 미숙아인 저출생 체중아를 대상으로 전신성 칸디다증의 임상증상과 증상 발현시의 검사 소견을 전신성 세균 감염증과 비교함으로써 이들 소견의 조기 감별 진단 상의의를 조사하였다. 방 법 : 1997년 1월부터 2001년 6월까지 경북대학교병원 신생아 집중치료실에 입원했던 미숙아 중 저출생 체중아 중에서 배양검사로 확인된 전신성 칸디다증 20례와 전신성 세균 감염증 23례를 대상으로 하여 이들의 의무 기록을 후향적으로 조사하였다. 결 과: 전신성 칸디다증 환아(이하 칸디다군)의 출생시 체중은 $1,290{\pm}290g$으로 전신성 세균 감염증 환아(이하 세균군)의 $1,530{\pm}430g$보다 유의하게 작았으며, 총 정맥 영양, 항생제 투여, 중심 카테테르, 기관 삽관, $H_2$ 차단제 사용의 빈도가 더 높았다. 임상 양상에 있어서는 서맥 동반 무호흡 및 호흡 곤란이 두 군의 주된 발현시 증상으로, 발현 빈도는 칸디다군(90%)에서 세균군(61%)에 비해 높았다. 증상 발현시 시행한 검사 소견에서 혈소판 감소증과 백혈구 감소증이 동반된 경우가 칸디다군 7례(35%), 세균군 2례(9%)로 두 군 사이에 유의한 차이를 보였다(P=0.03). 균은 대부분 혈액에서 배양되었으며(칸디다군 19례, 세균군 19례), 그 외에 요, 중심 카테테르, 뇌척수액 등에서 배양되었다. 감염균은 칸디다군은 Candida albicans가 15례, 세균군은 Klebsiella pneumoniae와 Enterobacter cloacae가 각각 5례로 가장 빈번하였다. 결 론 : 신생아 집중치료실에 장기 입원 중 전신 감염증 증상을 보이는 환아에서 호흡기 증상과 함께 검사상 혈소판 감소 단독 또는 혈소판 감소증과 백혈구 감소증이 동반된 경우에는 전신성 칸디다 감염증 가능성이 높을 것으로 사료된다. Purpose : Long term hospitalized infants in neonatal intensive care units(NICUs) are prone to systemic infection. It is important to differentiate systemic candidiasis from systemic bacterial infection early in the course. Thus, in this study, we have compared clinical characteristics of systemic candidiasis and systemic bacterial infection, in premature low birth weight infants. Methods : Retrospective chart review of the medical records of 20 patients with systemic candidiasis and 23 patients with systemic bacterial infection was performed. Results : Among the risk factors of systemic candidiasis, total parenteral nutrition(TPN), the use of broad spectrum antibiotics, central catheter insertion, endotracheal intubation and the use of H2 blockers were more frequent in neonates with systemic candidiasis than neonates with systemic bacterial infection. Apnea with bradycardia developed more frequently in neonates with systemic candidiasis compared with systemic bacterial infection(75% vs 39%). In laboratory findings at symptom onset, seven cases(35%) of systemic candidal infections and two cases(9%) of systemic bacterial infections showed leukopenia and thrombocytopenia(P=0.03). Blood was the most frequent isolation site of candida and bacteria. Conclusion : In neonates with systemic candidiasis, apnea with bradycardia, pneumonia and thrombocytopenia were prone to develop more frequently. The use of TPN, antibiotics and central catheters was strongly associated with systemic candidiasis. Empirical treatment with antifungal agent should be considered in critically ill neonates with above findings.

범불안장애의 불안증상에 대한 가미소요산의 제형별 효능 비교 임상연구의 중간보고

임정화 ( Jung Hwa Lim ),서복남 ( Bok Nam Seo ),이상룡 ( Sang Ryong Lee ),정인철 ( In Chul Jung ) 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.3

Objectives: The purpose of this research is to examine the efficacy of Gamisoyosan on anxiety of generalized anxiety disorder according to dosage form. Methods: In this randomized, double blinded, placebo-controlled study, we planned to give Gamisoyosan simple extract mixture or Gamisoyosan compound extract or controlled medication for major symptoms of generalized anxiety disorder. As preparatory research, Hamilton rating Scale for Anxiety(HAM-A) was measured as the 1st evaluative instrument, and Korean State-Trait Anxiety Inventory(STAI-K), Penn State Worry Questionnaire(PSWQ) Korean Beck`s Depression Inventory (BDI-K), Symptom Checklist-90-Revised(SCL-90R), WHO Quality of Life Abbreviated(WHOQOL-BREF) and Heart Rate Variability(HRV) were also measured as the 2nd evaluative instrument at the before treatment. Results: Demographic characteristics showed that there are Clinical characteristics-vital signs are within the normal range. The characteristics of disease-chief complaint, pattern Identification and etiological factor of the highest frequency number were worry, heart deficiency with timidity(心膽虛怯), family matters. The average period of disease in subjects is 6.31years. The results of Chest PA, EKG and clinicopathologic examination are within the reference range. The Scores of HAM-A, STAI-K, PSWQ were measured above the cutoff point. There are significant positive correlations among HAM-A, STAI-K, BDI-K and among HAM-A, STAI-K, PSWQ. There are no significant correlations between PSWQ and BDI-K. Conclusions: We considered that selection of subjects in this research is appropriately accomplished And this methodology is expected to be applied to the subsequent research. And also, we hope to make up for this study through various study and discussion.

聰明湯과 巴戟天聰明湯 열수추출물, 초미세분말제형이 microgli 및 기억력 감퇴 병태모델에 미치는 영향

임정화(Jung Hwa Lim),정인철(In Chul Jung),이상룡(Sang Ryong Lee) 한의병리학회 2006 동의생리병리학회지 Vol.20 No.4

두침(頭鍼)을 병행한 한방치료(韓方治療)를 통한 진전(振顫) 환자(患者) 4례(例)에 대한 증례 보고

임정화 ( Jung Hwa Lim ),정인철 ( In Chul Jung ),이상룡 ( Sang Ryong Lee ) 대한한방신경정신과학회 2004 동의신경정신과학회지 Vol.15 No.2

팔미합총명탕(八味合聰明湯) 열수추출물, 초미세분말제형이 Alzheimer`s Disease 병태 모델에 미치는 영향

임정화 ( Jung Hwa Lim ),이상룡 ( Sang Ryong Lee ) 대한한방신경정신과학회 2008 동의신경정신과학회지 Vol.19 No.2

Objective : This experiment was designed to investigate the effects of the PMCMT hot water extract & ultra-fine powder on Alzheimer`s Disease Model Induced by βA. Method : The effects of the PMCMT hot water extract on expression of proinflammatory cytokine mRNA in BV2 microglial cell cell line treated by lipopolysacchaide(LPS). The effects of the PMCMT hot water extract & ultra-fine powder on (1) the behavior (2) AChE in serum (3) the infarction area of the hippocampus, and brain tissue injury in Alzheimer`s diseased mice induced with βA were investigated. Result : 1. The PMCMT hot water extract suppressed the expression of proinflammatory cytokine mRNA in BV2 microglial cell line treated with LPS. 2. The PMCMT hot water extract & ultra-fine powder a significant inhibitory effect on the memory deficit was shown for the mice with Alzheimer`s disease induced by βA in the Morris water maze experiment, which measured stop-through latency and distance movement-through latency 3. The PMCMT hot water extract & ultra-fine powder suppressed the over-expression of AChE activity in the serum of the mice with Alzheimer`s disease induced by βA. 5. The PMCMT ultra-fine powder reduced infarction area of hippocampus significantly, and the PMCMT hot water extract & ultra-fine powder controlled the injury of brain tissue in the mice with Alzheimer`s disease induced by βA. Conclusions : These results suggest that the PMCMT hot water extract & ultra-fine powder may be effective for the prevention and treatment of Alzheimer`s disease. Investigation into the clinical use of the PMCMT hot water extract & ultra-fine powder for Alzheimer`s disease is suggested for future research.

In vitro에서 β-site amyloid precursor protein-cleaving enzyme 활성화 amyloid β protein 생산에 대한 총명탕가미방(聰明湯加味方)

임정화 ( Jung Hwa Lim ),정인철 ( In Chul Jung ),임종순 ( Jong Soon Lim ),김승형 ( Seung Hyung Kim ),이상룡 ( Sang Ryong Lee ) 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.2

This experiment was designed to investigate the effect of Chongmyung-Tang Prescription Combination(CmTP-C1-10) extract on the production of amyloid β protein and β-site amyloid precursor protein-cleaving enzyme(BACE) activity. Methods: The effect of CmTP-C1-10 extract on expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by lipopolysacchride(LPS) and amyloid β protein fragment(Aβ fragment) were investigated. The effect of CmTP-C1-10 extract on production of amyloid β protein(Aβ) in BV2 microglia cell line treated by LPS and Aβ fragment were investigated. The effect of CmTP-C1-10 extract on BACE activity were investigated. Results: 1. CmTP-C9 extract the most significantly suppressed the expression of APP mRNA, BACE2 mRNA in BV2 microglia cell line treated by LPS and Aβ fragment. 2. CmTP-C9 extract significantly suppressed the production of Aβ in BV2 microglia cell line treated by LPS and Aβ fragment. 3. CmTP-C9 extract the most significantly inhibited BACE activity. Conclusions: These results suggest that CmTP-C9 may be effective for the prevention and treatment of Alzheimer`s Disease, Investigation into clinical use of CmTP-C9 for Alzheimer`s Disease is suggested for future research.

뇌파를 이용한 생기능자기조절(뉴로피드백) 훈련에 대한 임상연구 동향 - 2000년부터 2013년까지 국내 학술지 논문을 중심으로 -

임정화 ( Jung Hwa Lim ),성우용 ( Woo Young Seong ) 대한한방신경정신과학회 2014 동의신경정신과학회지 Vol.25 No.3

Objectives: The purpose of this study is to investigate the clinical trial research trends in regards to the neurofeedback training (NFT) in Korean journals. Methods: We researched articles published in Korean journals from 2000 up to 2013 and were related to the NFT. We searched six electronic databases to find relevant articles, using the term ‘neurofeedback’. Results: 1) 6 single group comparative studies and 31 randomized controlled trials were found. 2) Healthy volunteers and students were most frequently studied with neurofeedback training. Other studies included attention deficit hyperactivity disorder (ADHD), Poststroke, panic disorder, premenstrual dysphoric disorder (PDD), temporomendibular disorder (TMD), and obesity. NFT interventions were attempted in diverse training protocols and assessed with many different outcome measurements. 3) Most studies showed effective results after NFT. Conclusions: NFT is increasingly studied and used in various clinical fields. Also, there have been efforts to adopt NFT in Korean medical clinics and researches, and more rigorous and innovative studies are needed in the future.

독서요법과 뉴로피드백을 시행한 심비양허형(心脾兩虛型) 울증(鬱證)환자 치험(治驗) 1례(例)

임정화 ( Jung Hwa Lim ),최강욱 ( Kang Wook Choi ),정인철 ( In Chul Jung ),이상룡 ( Sang Ryong Lee ) 대한한방신경정신과학회 2006 동의신경정신과학회지 Vol.17 No.2

Bibliotherapy is the field of clinical counceling to treat the emotional, psychiatric and social maladjustment. Neurofeedback is a means by which participants can learn voluntary control of the EEG and has been applied to a range of clinical conditions such as epilepsy, attention deficit hyperactivity disorder, depressive disorder. ``Ul-zeong`` comes from obstruction of qi by stress. The mind affect the body. The patient has depressed mood, irritable sign, chest discomfort, flank pain, angry state and some strange feeling on the throat. In this case, a female patient, 30 years old, who complained of amnesia, deficiency of the power of the attention and concentration depersonalization, depressed mood, insomnia ect. We treated the patient with bibliotherapy, neurofeedback and oriental medical treatment such as heral medicine, acupuncture treatment and aroma therapy. In result, the symptoms which she complained were improved.

ACM의 비글견을 이용한 단회 경구투여 용량증가 독성 시험 및 4주 반복 경구투여 용량 결정 시험

임정화 ( Jung Hwa Lim ),이상룡 ( Sang Ryong Lee ),정인철 ( In Chul Jung ) 대한한방신경정신과학회 2013 동의신경정신과학회지 Vol.24 No.1

Objectives: To provide information on the safety of ACM, we carried out a single oral dose-increasing toxicity and 4-weeks repeated oral dose determining test of ACM in beagle dogs. Methods: In a single oral dose-increasing toxicity test, beagles were treated with ACM orally increasing dose level (1,000, 2,000, 5,000 mg/㎏) at interval of 3 days. After administration, signs of toxicity were observed for two weeks. In 4-weeks repeated oral dose determinating test, beagles were treated with ACM with oral dose 500, 1,000, 2,000 mg/kg for 4 weeks. Mortality, clinical signs, body weight changes, food consumption, urinalysis, hematological and biochemical parameters, organ weights, necropsy findings, and histological findings were monitored during the study period. Results: In a single oral dose-increasing toxicity test, we found no mortality, abnormalities in clinical signs, body weight, and necropsy findings during the study period. In 4-weeks repeated oral dose determinating test, we found no mortality, abnormalities in clinical signs, body weight, food consumption, urinalysis, hematological and biological parameters, gross findings, organ weights, necropsy findings, and histopathological findings in any of the beagles tested. Conclusions: The results obtained in these studies suggest that maximum tolerated dose (MTD) of ACM in male and female beagle dogs was supposed to be over 5,000 mg/kg. For the future studies of toxicity, it is advisable that high dose and low dose are set at 2000 mg/kg and 500 mg/kg, respectively.