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HSP27 EXPRESSION IN OSTEOBLAST BY THERMAL STRESS
임재석,김병렬,권종진,장현석,이의석,전상호,우현일,Rim, Jae-Suk,Kim, Byeong-Ryol,Kwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Suk,Jun, Sang-Ho,Woo, Hyeon-Il Korean Association of Maxillofacial Plastic and Re 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.1
Aim of the study: Thermal stress is a central determinant of osseous surgical outcomes. Interestingly, the temperatures measured during endosseous surgeries coincide with the temperatures that elicit the heat shock response of mammalian cells. The heat shock response is a coordinated biochemical response that helps to protect cells from stresses of various forms. Several protective proteins, termed heat shock proteins (hsp) are produced as part of this response. To begin to understand the role of the stress response of osteoblasts during surgical manipulation of bone, the heat shock protein response was evaluated in osteoblastic cells. Materials & methods: With primary cell culture studies and ROS 17/2.8 osteoblastic cells transfected with hsp27 encoding vectors culture studies, the thermal stress response of mammalian osteoblastic cells was evaluated by immunohistochemistry and western blot analysis. Results: Immunocytochemistry indicated that hsp27 was present in unstressed osteoblastic cells, but not fibroblastic cells. Primarily cultured osteoblasts and fibroblasts expressed the major hsp in response to thermal stress, however, the small Mr hsp, hsp27 was shown to be a constitutive product only in osteoblasts. Creation of stable transformed osteoblastic cells expressing abundant hsp27 protein was used to demonstrate that hsp27 confers stress resistance to osteoblastic cells. Conclusions: The demonstrable presence and function of hsp27 in cultured bones and cells implicates this protein as a determinant of osteoblastic cell fate in vivo.
하악 제 3대구치의 치근 만곡도와 매복정도의 관계 분석
임재석(Jae-Suk Rim),권종진(Jong-Jin Kwon),장현석(Hyun-Seok Jang),박영준(Young-Joon Park),전상호(Sang-Ho Jun),현승돈(Seung-Don Hyun) 대한구강악안면외과학회 2002 대한구강악안면외과학회지 Vol.28 No.1
Purpose : We examined the relationships between the root curvatures of the mandibular third molars and their locations on the mandible, so that it might be contributed to elucidate the origin of the formation and development of the Korean mandibular third molar and help many clinicians to treat the mandibular third molars. Materials and Methods : Panoramic radiographs of 237 patients who had been treated at Korea University Ansan Hospital from March, 2000 to April, 2001 and had bilateral mandibular third molars were examined and analyzed. Results : There was no statistically significant difference between men and women (p<0.05). But there were significantly fewer subjects with bilateral curved roots in bilateral third molars regardless of their status (p<0.01) and were statistically significant differences between the groups with bilateral curved roots in the mandibular third molars with same status and with unilateral curved roots in the mandibular third molars with different status and between the groups with unilateral curved roots in the mandibular third molars with same status and with bilateral curved roots in the mandibular third molars with different status(p<0.05). Subjects with bilateral curved roots in the mandibular third molars with bilateral partial impacted status and with bilateral curved roots in the mandibular third molars with bilateral complete eruption status were significantly fewer (p<0.01) and subjects with the mandibular third molars with partial impacted and complete impacted status and with the mandibular third molars with complete erupted and partial impacted status had significantly fewer bilateral curved roots (p<0.05). Conclusions : When we postulate that it is genetic and environmental factors that affect the root formation, it seems that environmental factors have more effects on the formation of the mandibular third molars than on that of the mandibular first and second molars
PLEIOTROPHIN (PTN) EXPRESSION IN OSTEOBLASTIC CELLS
김병렬,임재석,권종진,장현석,이의석,전상호,김영진,Kim, Byeong-Yol,Rim, Jae-Suk,Kwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Seok,Jun, Sang-Ho,Kim, Young-Jin Korean Association of Maxillofacial Plastic and Re 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.6
Pleiotrophin or osteoblast-specific factor 1(HOSF-1) is a growth-associated protein present in bone matrix. This study was designed to study pleiotrophin expression in osteoblastic cells. Pleiotrophin was expressed by osteoblast-like cell line. Pleiotrophin expression increased following the proliferative phase and was minimal at the terminal phases of the induced differentiation of cultured MC3T3-E1 cells. Pleiotrophin expression represents another autocrine factor that may contribute to the physiologic control of induced bone formation. In this study, induced osteogenesis will be examined in the context of the osteoblast expression of and regulation by PTN. I hypothesized that PDGF-BB stimulation of PTN expression represents an important paracrine signal during the induced osteogenesis associated with periodontal and implant surgeries. The possible mediation by PTN of anabolic effects attributed to PDGF-BB stimulation was examined in cell culture models of osteoblast differentiation. These studies will contribute fundamental insights to osteoblast biology and insights regarding the potential use of factors such as PTN in the clinical environment.
이바다,권진일,임재석,백지웅,박진후,김형준,Lee, Bada,Kwon, Jin-Il,Lim, Jae-Seok,Baek, Jiwoong,Park, Jin Hoo,Kim, Hyung Jun 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.6
Traumatic lacerations are common in the orofacial region as a result of accidents. Due to the frequent movement of the skin around the mandible, scars in that area are more likely to widen or become hypertrophic. Treatment of facial laceration was performed on three patients and followed by regular check-up. It was discovered that the scars have become hypertrophic, so steroid injections were used. The results were satisfactory with the decrease in sizes and hardnes of the scar. Facial scarring is a particularly distressing phenomenon and has always been a challenge to treat as the scars are more likely to widen or become hyphertrophied due to the frequent movement of the muscle in the facial area. We confirmed that the positive effect of steroid on hypertrophic scars. So, we suggest that proper treatment and periodic follow-up, adjuvant treatments especially steroid injection is necessary in patients with lacerations.
POSTOPERATIVE TOXIC SHOCK SYNDROME: REPORT OF A CASE
장현석,권종진,임재석,이갑재,Jang, Hyon-Seok,Kwon, Jong-Jin,Lim, Jae-Seok,Lee, Kab-Jae Korean Association of Maxillofacial Plastic and Re 1999 Maxillofacial Plastic Reconstructive Surgery Vol.21 No.1
Toxic Shock Syndrome(TSS)은 1978년 Todd등에 의해 처음 보고된 이래 젊은 여성에서 월경과 연관된 질환으로 생각되어 왔으나 최근에는 코수술을 비롯한 두경부수술 및 기타 소수술후 합병증으로 많은 증례가 발표되고 있다. 이의 진단은 주로 임상적 진단, 즉 갑작스런 술후 고열, 설서, 저혈압 및 홍피증과 혈액배양등에서 검출되는 포도상구균등으로 가능하다. TSS은 대부분 수액공급 및 혈압의 유지, 전신적인 항생제 투여등으로 쉽게 치료가 가능하지만, 경과가 좋지 않은 경우 면역글로불린의 사용도 시도되어 좋은 결과를 얻고 있으며 조기 발견 및 처치가 가장 중요하다고 할 수 있다. 이에 저자는 상악골 및 비골, 안와근심벽 골절을 지닌 48세 남자환자에서 골절부에 대한 관혈적 정복고정술후 발생된 TSS의 처치에 대해 문헌고찰과 함께 보고하는 바이다. Toxic shock syndrome is an acute disease involving multiple organs. I described here a case of TSS associated with intranasal packing. Four major criteria, which are involvement of multiple organ systems, fever, hypotension or shock, and rash with subsequent desquamation should be fulfilled for the diagnosis. The exact pathogenesis is not well understood, however it is thought to be due to the effects of an enterotoxin produced by certain strains of staphylococcus aureus.
HSP27 MODULATION OF IMPLANT- ASSOCIATED METAL ION CYTOTOXICITY OF OSTEOBLASTIC CELLS
윤정호,하동진,임재석,권종진,장현석,이의석,김대성,Yoon, Jung-Ho,Ha, Dong-Jin,Rim, Jae-Suk,Kwon, Jong-Jin,Jang, Hyon-Seok,Lee, Eui-Seok,Kim, Dae-Sung Korean Association of Maxillofacial Plastic and Re 2006 Maxillofacial Plastic Reconstructive Surgery Vol.28 No.2
Objectives: The extent of bone formation that occurs at the interface of metallic implants and bone is determined by the number and activity of osteoblastic cells. Stress proteins may be contributing determinants of cell viability in altered environments. Hsp27 is a small Mr hsp which is known as a molecular chaperone. Methods: To better understand how heat shock protein 27 contributes to endosseous implant - associated metal ions affects on osteoblastic cell viability, the effect of chromium and titanium ions were compared to effects of cadmium ions in the ROS17/2.8 osteoblastic cell line. Results: ROS17/2.8 osteoblastic cell line demonstrated ion - specific reductions in growth; reductions were significantly greater for cadmium than for chromium or titanium. Chromium impaired growth of cultures without altering cell viability measured using the MTT assay. A stable transformed cell line expressing additional hsp27(clone "A7") was resistant to the toxic effects of titanium and partially protected from cadmium toxicity. Conclusions: A role for hsp27 in protection of osteoblastic cells from metal ion toxicity is supported by the chromium - induced elevations in hsp27 abundance and the behavior of the A7 cell line in response to metal ions in culture. Similar biochemical responses to altered cellular environments may contribute to the fate of tissues adjacent to select metallic implants.
지방조직 유래 줄기세포의 조골세포로의 분화에 대한 실험적 연구
이의석,장현석,권종진,임재석,Lee, Eui-Seok,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2008 Maxillofacial Plastic Reconstructive Surgery Vol.30 No.2
Stem cells have self-renewal capacity, long-term viability, and multiline age potential. Adult bone marrow contains mesenchymal stem cells. Bone marrow-derived mesenchymal stem cells (BMSCs) are progenitors of skeletal tissue components and can differentiate into adipocytes, chondrocytes, osteoblasts, and myoblasts in vitro and undergo differentiation in vivo. However, the clinical use of BMSCs has presented problems, including pain, morbidity, and low cell number upon harvest. Recent studies have identified a putative stem cell population within the adipose tissue. Human adipose tissue contains pluripotent stem cells simillar to bone marrow-derived stem cells that can differentiate toward the osteogenic, adipogenic, myogenic, and chondrogenic lineages. Human adipose tissue-derived stem cells (ATSCs) could be proposed as an alternative source of adult bone marrow stem cells, and could be obtained in large quantities, under local anesthesia, with minimal discomfort. Human adipose tissue obtained by liposuction was processed to obtain ATSCs. In this study, we compared the osteogenic differentiation of ATSCs in a specific osteogenic induction medium with that in a non-osteogenic medium. ATSCs were incubated in an osteogenic medium for 28 days to induce osteogenesis respectively. Osteogenic differentiation was assessed by von Kossa and alkaline phosphatase staining. Expression of osteocyte specific bone sialoprotein, osteocalcin, collagen type I and alkaline phosphatase, bone morphogenic protein 2, bone morphogenic protein 6 was confirmed by RT-PCR. ATSCs incubated in the osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. Expression of osteocyte specific genes was also detected. Since this cell population can be easily identified through fluorescence microscopy, it may be an ideal source of ATSCs for further experiments on stem cell biology and tissue engineering. The present results show that ADSCs have an ability to differentiate into osteoblasts. In the present study, we extend this approach to characterize adipose tissue-derived stem cells.
가토 두개골 결손부에서 베타-삼칼슘 인산염 이식 시 혈소판 풍부 섬유소와 혈소판 풍부 혈장의 골형성능에 관한 연구
박정균,주현중,이의석,장현석,임재석,권종진,Park, Jeong-Kyun,Joo, Hyun-Jung,Lee, Ei-Seok,Jang, Hyon-Seok,Lim, Jae-Seok,Kwon, Jong-Jin 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.1
Purpose: Addition of platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) to grafting material has become widely accepted additively for bone regeneration because it can raise high expectations on it's clinical potential. The aim of this study was to evaluate the efficacy of PRP and PRF on early bone regeneration of rabbits when used in combination with beta tricalcium phosphate. Methods: In eight rabbits, the calvarium was exposed and the two marrows were penetrated. After then these artificial bone defects were augmented with ${\beta}$-TCP or ${\beta}$-TCP with PRP or ${\beta}$-TCP with PRF and covered. The animals were sacrificed after four and eight weeks. Histologic findings were observed under the light-microscope and histomorphometric analysis was performed by measuring calcified area of new bone formation within the CSD. Results: They demonstrated that new bone formation tended to be produced along the outline of graft materials. More amounts of newly bone was regenerated in ${\beta}$-TCP only and in combination of${\beta}$-TCP with PRF and it was statistically significant. In contrast, there was no significant difference between nothing apply and ${\beta}$-TCP with PRP groups in the relative amounts of newly mineralized bone. Conclusion: Within the limitation of this study, it can be concluded that PRF in combination with ${\beta}$-TCP showed a positive effect on bone regeneration and statistically it was significant.