ASC-H 환자의 p16INK4a 면역세포화학염색 검사의 유용성

임광일,임광일,강여주,김태은,박경신,정은선,최영진,이교영,강창석,이아원 대한병리학회 2011 Journal of Pathology and Translational Medicine Vol.45 No.3

Background: The grey zone of cervical cytology, and in particular atypical squamous cells, can­not exclude HSIL (ASC-H) causes diagnostic difficulties and increases medical expenses. We an­alyzed p16INK4a expression in ASC-H liquid-based cytology specimens (LBCS) to develop more effective methods for the management of ASC-H patients. Methods: We carried out p16INK4a im­munostaining with 57 LBCS of ASC-H diagnostic categories, all of which were histologically co­firmed and 43 cases of which were compared with the results of a human papillomavirus (HPV) chip test. Results: p16INK4a immunostaining with ASC-H LBCS was positive in 20% (3/15) of cer­vicitis, 25.0% (3/12) of tissue-low-grade squamous intraepithelial lesion, 75.0% (18/24) of tissue-high grade squamous intraepithelial lesion (HSIL), and 100% (6/6) of invasive cancer cases. The positivity of p16INK4a in LBCS was correlated with higher grade of histologic diagnosis (r=0.578, p=0.000). The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of p16INK4a immunostaining for the prediction of tissue-HSIL+ were 80.0%, 77.8%, 80.0%, and 77.8%, respectively. The sensitivity, specificity, PPV, and NPV of p16INK4a immunostaining plus HPV chip test for predicting tissue-HSIL+ were 71.2%, 86.4%, 84.2%, and 79.2%. Conclu­sions: p16INK4a immunostaining as well as HPV chip testing with remaining LBCS with ASC-H are useful objective markers for the prediction of tissue-HSIL+.

폐신문지(廢新聞紙)(ONP)로부터 부유선별(浮遊選別)을 이용한 잉크분리(分離)시 초음파처리(超音波處理) 효과(效果)

임광일,황인성,김정운,유광석,안지환,한춘,Lim, Gwang Il,Hwang, In Sung,Kim, Joung Woon,You, Kwang Suk,Ahn, Ji Whan,Han, Choon 한국자원리싸이클링학회 2012 資源 리싸이클링 Vol.21 No.5

재생률이 높은 폐신문지(old newspaper, ONP)를 부유선별법을 이용하여 탈묵을 진행하였을 시, 해리시간, 초음파처리에 따른 백색도, 잔류잉크농도(effective residual ink concentration, ERIC), 수율의 효과를 규명하였다. 또한 부유선별시 pH, 포수제에 따른 백색도, ERIC과 수율의 효과도 규명하였다. 해리시간이 증가할수록 백색도와 ERIC이 증가함을 보였으나 수율의 차이는 없었다. 해리시간이 30분일 때 백색도 58% ERIC 182.9 ppm이였고, 해리를 더 진행하더라도 백색도의 증가를 볼 수 없었다. 단 해리 된 ONP를 초음파발생장치를 이용하여 초음파처리를 했을 경우 단 2분의 해리시간으로도 58%이상의 백색도를 얻을 수 있었다. 초음파처리를 20분간하고, pH 11에서 포수제로 등유을 사용했을 때 백색도가 59.32%로 가장 높았다. In this study, the sonication method was applied to remove ink from old newspaper(ONP) using flotation. Results indicated increased whiteness and effective residual ink concentration (ERIC) with longer disintegration. Therefore 58% of whiteness and 182.9 ppm ERIC were obtained for 30 minutes of disintegration time without the change in yield. Longer disintegration than 30 minutes had no effects on whiteness without the application of ultra-sonication. On the other hand, higher than 58% of whiteness was achieved in 2 minutes of disintegration when the ultra-sonication was applied. The highest whiteness (59.32 %) was obtained by applying sonication for 20 minutes and froth flotation with kerosene as a collector under the alkaline condition (pH 11).

Development of safe gene delivery viral vectors

임광일 한국공업화학회 2021 한국공업화학회 연구논문 초록집 Vol.2021 No.0

선형예측 음성부호화에서의 음원모델링에 관한 연구

임광일,이태원,이승훈,이규호 고려대학교 공학기술연구소 1990 고려대학교 생산기술연구소 생기연논문집 Vol.26 No.1

The research for speech quality improvement of the linear predictive vocoding is under way in two points of view: an accurate extraction of vocal tract parameter and a modeling of excitation signal in a speech production model. This paper proposes the new hybrid excitation model for linear predictive vocoding which gives an improved speech quality over that of a conventional excitation model, and an evident data compression effect over that of a typical multi-pulse excitation model. To verify the validity of the proposed model, a computer simulation is carried out. It is observed that the proposed excitation model in vocoding performs an improved speech quality and a higher data compression effect.

합성(合成) 추출(抽出) 수지(樹脂)를 이용(利用)한 백금용액(白金溶液)으로부터 알루미늄과 철(鐵)의 분리(分離)

임광일,한동혁,황인성,한춘,이진영,김준수,박형규,Lim, Gwang-Il,Han, Dong-Hyuk,Hwang, In-Sung,Han, Choon,Lee, Jin-Young,Kim, Joon-Soo,Park, Hyung-Kyu 한국자원리싸이클링학회 2011 資源 리싸이클링 Vol.20 No.3

수지합성 시 추출제의 고른 분산과 균일한 입자를 만들어 알루미늄과 철의 추출량을 증가시키고자 반응 개시제인 benzoyl peroxide(BPO)의 첨가법을 변화시킨 결과 세 번 나누어 첨가한 3pot의 성능이 가장 우수하였다. D2EHPA를 이용하여 합성한 수지의 경우 Fe와 Pt간에 Fe는 100% 추출되었으며, Al과 pt간의 분리실험에서 99.9%의 Al이 추출되었다. 수입되는 $P_{204}$ 수지 역시 Fe와 Pt간의 분리실험에서 100%의 Fe가 추출되었으며 Al과 Pt간의 분리실험에서는 98.9%의 Al이 추출되는 것을 확인하였다. 합성수지와 $P_{204}$내 추출제 함량은 각각 61.8, 60%이며, 이러한 차이가 추출효율에 차이를 가져오는 것으로 판단된다. 한편 두 수지 모두 $55^{\circ}C$까지는 Al과 Fe의 추출효율이 증가하나, 그 이상의 온도에서는 영향이 없는 것으로 확인되었다. FT-IR분석 결과 추출제에 따른 고유의 피크를 $1000cm^{-1}$에서 확인할 수 있었으며 합성한 추출 수지와 $P_{204}$ 수지 모두 $2900cm^{-1}$에서 가교 폴리스틸렌의 특징적인 피크를 확인하였다. For the separation of aluminum and iron from platinum mixtures, extraction resins were synthesized and separation efficiencies were compared with those by commercial one, $P_{204}$. During synthesis, the suspension polymerization method was adopted with D2EHPA as an extractant. Also, benzoyl peroxide as a starter was divided into 3parts and injected for the uniform size and dispersion of resin particles. Comparison tests resulted in 100% separation of Fe and Pt for both synthetic and $P_{204}$ resins. In case of Al and Pt, synthetic and $P_{204}$ resin gave extraction efficiencies of 99.9% and 98.9%, respectively. Difference in extractant contents of synthetic resin(61.8%) and $P_{204}$(60%) was considered to give differences in separation efficiencies of aluminum and iron elements. For both resins, separation efficiencies of Al and Fe increased up to $55^{\circ}C$. According to FT-IR analyses of both resins, specific peaks of D2EHPA and crosslinked polystyrene were identified at the wavenumber of $1000cm^{-1}$ and $2900cm^{-1}$ respectively.

Miscibility of chitosan/starch blends in solution by viscosity and refractive index methods

임광일,나재식 한국고분자학회 2009 한국고분자학회 학술대회 연구논문 초록집 Vol.2009 No.4

Physical properties of Silicone Acrylic Resins Coating

임광일,김성길,나재식 한국고분자학회 2009 한국고분자학회 학술대회 연구논문 초록집 Vol.2009 No.4

Retroviral Infection of hES Cells Produces Random-like Integration Patterns

임광일 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.5

Retroviral integration provides us with a powerful tool to realize prolonged gene expressions that are often critical to gene therapy. However, the perturbation of gene regulations in host cells by viral genome integration can lead to detrimental effects, yielding cancer. The oncogenic potential of retroviruses is linked to the preference of retroviruses to integrate into genomic regions that are enriched in gene regulatory elements. To better navigate the double-edged sword of retroviral integration we need to understand how retroviruses select their favored genomic loci during infections. In this study I showed that in addition to host proteins that tether retroviral pre-integration complexes to specific genomic regions, the epigenetic architecture of host genome might strongly affect retroviral integration patterns. Specifically, retroviruses showed their characteristic integration preference in differentiated somatic cells. In contrast, retroviral infections of hES cells, which are known to display decondensed chromatin, produced random-like integration patterns lacking of strong preference for regulatory-element-rich genomic regions. Better identification of the cellular and viral factors that determine retroviral integration patterns will facilitate the design of retroviral vectors for safer use in gene therapy.

Design and Implementation of Digital Signal Processor and Development System

임광일,이우선,신인철,이태원,Lim, Kwang Il,Lee, Woo Sun,Shin, In Chul,Rhee, Tae Won The Institute of Electronics and Information Engin 1986 전자공학회논문지 Vol.23 No.6

A real-time microprogrammable digital signal processor is designed and implemented using the bit-slice logic, a parallel multiplier, 74 series TTLs and MOS memories. A microinstruction set for the processor is defined and an application program development system is constructed. For its performance evalution, a digital filter and FFT are implemented with this digital signal processor. It is proved that this processor is faster than commrcially available single chip digital signal processors such as \ulcornerD 7720, AMI 2811, enabling very high speed digital signal processing.

Image Analysis of HER2 Immunohistochemical Staining of Surgical Breast Cancer Specimens

임광일,박홍식,김동민,이연수,이아원 연세대학교의과대학 2019 Yonsei medical journal Vol.60 No.2

Purpose: Trastuzumab is an effective treatment for human epidermal growth factor receptor 2 (HER2)-amplified breast cancers. We sought to develop a simple protocol for HER2 image analysis of breast cancer specimens. Materials and Methods: In a preliminary test, we found that at least 1000 tumor cells need to be examined in the most stronglystained areas. Next, we evaluated the clinical usefulness of this established protocol of image analysis in 555 breast cancer patients. Results of the HER2 immunohistochemical (IHC) staining were compared between manual scoring and image analysis. Results: The HER2 IHC results obtained by the image analysis method correlated well with those obtained by the manual scoringmethod (Cohen’s kappa=0.830). Using the HER2 silver in situ hybridization (SISH) results as a gold standard, sensitivity valueswere 72.1% for manual scoring and 74.0% for image analysis; specificity values were 96.2% for manual scoring and 94.7% for imageanalysis; and accuracy values were 91.7% for manual scoring and 90.8% for image analysis. McNemar’s test was applied to theresults, and there were no statistically significant differences in sensitivity and specificity between the positive (p=0.688) and negative(p=0.118) SISH groups. Conclusion: HER2 image analysis results were similar to those obtained via the manual scoring method, indicating that the useof image analysis can reduce assessment time and effort. We suggest that image analysis-based evaluation of 1000 tumor cells inthe most strongly IHC-stained area, regardless of stroma content, is sufficient for determining HER2 expression levels in breastcancer specimens.