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      • KCI등재

        유색 찰옥수수의 항산화력 및 작물학적 특성

        이희봉,박보영,지희정,조진웅,김석형,모은경,이미라,Lee, Hee-Bong,Park, Bo-Young,Ji, Hee-Chung,Cho, Jin-Woong,Kim, Seog-Hyung,Mo, Eun-Kyung,Lee, Mi-Ra 한국작물학회 2006 Korean journal of crop science Vol.51 No.suppl1

        본 시험 결과를 요약하면 다음과 같다. 1. 공시교잡종의 평균 과피두께는 $37{\mu}m$로 얇은 특성을 보였는데 특히 CNU108 교잡종이 $30.3{\mu}m$로 가장 얇은 특성을 보였고 출사일수는 CNU69와 CNU202 등이 59일로 가장 빨랐으나 CNU160 교잡종은 68일로 본 교잡종들 중에서 가장 늦었다. 2. CNU70과 CNU138의 교잡종들은 높은 항산화효소(xanthin oxidase, catalase, superoxide dismutase)의 활성을 나타내었고, 특히 CNU160과 CNU193 교잡종은 각각 94.8%, 94.6% 등으로 높은 항산화력(EDA)을 나타내었다. 3. CNU109와 CNU34의 교잡종에서는 전반적으로 높은 항산화효소 활성 및 항산화력을 나타내었다. 따라서 CNU34, CNU70, CNU108, CNU138, CNU193 등의 교잡종 찰옥수수가 항산화 효과가 높은 고기능성 유색 찰옥수수인 것으로 사료되었다. This study was carried out to evaluate major agronomical characterization and phenol compound contents, Xanthine oxidase inhibitory activity (XO), Catalase activity, Superoxide dismutase activity (SOD) and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radical sacvenging activity were analyzed in colored waxy corns. The mean of stem height and ear length were 248.8 cm and 18.6 cm, respectively. The pericarp thickness in CNU108 $(30.3{\mu}m)$ was thinner than other hybrids. The period of tasseling days in CNU69 and CNU202 were very shorter than other hybrids (59 days). 100-kernel weight of CNU50 was 35.6 g and heavier than the others. The antioxidant activities such as xanthin oxidase (XO), catalase and superoxide dismutase (SOD) were higher in CNU70 and CNUI 38. Especially highest EDA (electron donating ability) in DPPH radical scavenging effect was 94.8% and 94.6% in CNU160 and CNU193, respectively. In the results, the antioxidant enzyme activity and antioxidant acticity were higher in CNU109 and CNU 34 hybrids. The hybrids, CNU34, CNU70, CNU108, CNU 138 and CNU193 may be considered higher functional color waxy corn.

      • 초음파 유도하 터널식 혈액투석용 도관 삽입술

        이희봉,장근조,Lee Hee Bong,Jang Kuhn Jo 대한방사선사협회 2001 대한방사선사협회지 Vol.27 No.2

        Ⅰ. Purpose : To evaluate the usefulness of ultrasound-guided, radiologic placement of tunneled hemodialysis catheters via internal jugular vein(IJV). Ⅱ. Materials and Methods : In 32 patients(31 chronic renal failure, 1 acute renal failure), We performed

      • KCI등재

        Call Admission Control using Soft QoS-based Borrowing Scheme in DVB-RCS Networks

        이희봉,장영민,이호진,Lee, Hee-Bong,Jang, Yeong-Min,Lee, Ho-Jin The Korean Institute of Communications and Informa 2005 韓國通信學會論文誌 Vol.30 No.2a

        본 논문에서는 DVB-RCS (Digital Video Broadcast-Return Channel via Satellite) 망에서 호 수락제어 (CAC)를 위한 Soft-QoS 기반의 borrowing 기법을 제안한다. 제안된 기법은 위성 망에서 사용 가능한 자원이 부족할 때 새로운 호를 받아들이기 위해 서비스 중인 호 중 일부에서 일시적이고 공평하게 대역폭을 가져온다. 각각의 호에서 가져오는 대역폭의 양은 각 사용자의 critical bandwidth ratio에 비례하며 critical bandwidth ratio는 제안된 기법에서 이용하는 QoS 파라메타 중 하나이다. 시뮬레이션 결과는 제안된 기법이 호의 blocking 확률과 대역폭 효율 측면에서 성능의 향상이 있음을 보여준다. We propose a soft QoS-based borrowing scheme for call admission control(CAC) in DVB-RCS(Digital Video Broadcast-Return Channel via Satellite). Some of the ongoing calls temporarily and fairly release bandwidths that can be used to accommodate a new call in an overloaded satellite network. The amount of bandwidth borrowed from each call is proportional to each user's critical bandwidth ratio, one of parameters for soft QoS mechanism. Simulation results show that the proposed scheme improves the system performance in terms of call blocking probability and bandwidth utilization.

      • KCI등재

        대추 (Zizyphus jujuba MILLER)의 저장중(貯藏中) 화학성분변화(化學成分變化)에 관(關)한 연구(硏究)

        이희봉,김성열,Lee, Hee Bong,Kim, Seung Yeol 충남대학교 농업과학연구소 1988 Korean Journal of Agricultural Science Vol.15 No.1

        건조(乾操)대추의 저장기간중(貯藏期間中)에 나타나는 갈변현성(褐變現象)과 화학성분(化學成分)의 변화(變化)를 검토(檢討)하기 위하여 4품종(品種)의 신선(新鮮)한 대추를 수집(蒐集)하여 일반화학성분(一般化學成分)을 분석(分析)하여 품종간(品種間)의 성분함량(成分含量)을 비교(比較)하고 이들중 보은(報恩)대추를 천일(天日) 건조(乾燥)하여 상온(常溫)에서 12개월(個月)동안 저장(貯藏)하면서 경시적(經時的)으로 색상(色相), 적정산도(滴定酸度), pH, vitamin C, 유기산(有機酸) 총(總) amino산(酸), 유리(遊離) amin산(酸), 유리당(遊離糖), hydroxymethyl furfural (HMF) 및 phenol 화합물(化合物)들을 분리정량(分離定量)하여 다음과 같은 결과(結果)를 얻었다. 1. 신선(新鮮)한 대추의 일반화학성분중(一般化學成分中) 수분(水分) 외(外)에는 당질(糖質)(22-28g)과 vitamin C(62-72mg)의 함량(含量)이 많았으며 품종간(品種間)에는 큰 차이(差異)가 나타나지 않았다. 2. 건조(乾操)대추의 갈색도(褐變度)는 저장기간(貯藏期間)에 따라 증자처리구(蒸煮處理區) 비처리구(非處理區) 모두 크게 증가(增加)되었다. 3. 신선(新鮮)한 대추의 vitamin C 함량(含量)은 297.4mg%DB이었으나 건조(乾操)한 후(後) 12개월(個月) 저장(貯藏)하였을 때는 20.2mg%DB로 약(約) 93%가 소실(消失)되었다. 4. 유기산(有機酸)은 oxalic acid, succinic acid, fumaric acid, malic acid, citric acid의 5종(種)이 동정(同定)되었고 저장중(貯藏中)의 함량변화(含量變化)는 fumaric acid만이 증가(增加)되었고 나머지 4종(種)의 유기산(有機酸)은 감소(減少)하였다. 5. 총(總) amino산(酸)은 17종(種)이 동정(同定)되었고 이들중(中) 주요(主要) amino산(酸)은 proline, threonine, glutamic acid, lysine이었으며 저장기간중(貯藏期間中) 총(總)aminol산(酸)은 약(約) 30% 감소(減少)되었다. 6. 유리(遊離) amino산(酸)은 threonine, proline, alanine, valine의 4종(種)이 동정(同定)되었으며 proline의 함량(含量)이 특(特)히 많았고 저장기간(貯藏期間中)중 평균(平均) 85%가 감소(減少)하였다. 7. 건조(乾操)대추의 유리당(遊離糖)은 fructose, glucose, sucrose로 구성(構成)되었 으며 12개월(個月) 저장(貯藏)하였을 때 유리당(遊離糖)의 총(總) 함량(含量)이 24% 감소(減少)하였으며 sucrose는 4개월(個月) 이후(以後)에는 검출(檢出)되지 않았다. 8. 저장중(貯藏中)의 대추에서 HMF를 분리동정(分離同定)하였으며 저장기간(財藏期間)에 따라 그 함량(含量)은 현저(顯著)하게 증가(增加)하였다. 9. 건조(乾燥)대추의 phenol 화합물(化合物)로는 caffeic acid, ferulic acid, P-coumaric acid의 3종(種)이 동정(同定)되었고 그 함량(含量)은 저장기간중(貯藏期間中)에 상당(相當)한 양(量)이 감소(減少)되었다. In order to find out changes of chemical components related to browning of dried jujube, four varieties were subjected to the proximate analysis. Boeun, one of the major varieties in Korea, was sun-dried and stored for 12 months and analyzed periodically for one year. Browning, titrable acidity, pH, contents of ascorbic acid, organic acid, total amino acid, free amino acid, free sugar, hydroxymethylfurfural (HMF) and phenolic compounds were determined and compared with those of fresh jujube. The results obtained are summarized as follows; 1. In comparison with other similar fruits, jujube was high contents of ascorbic acid (62-79mg%) and carbohydrate (22-28%) excluding fiber. 2. Browning was increased in both steam-treated and nontreated plot together as storage period was prolonged. 3. Ascorbic acid content of fresh jujube was as high as 297.4mg% DB, but it was decreased to 20.2mg% DB, after 12 months storage. Therefore, loss of ascorbic acid was very great up to 93% of its original content. 4. Five kinds of organic acid, oxalic, succinic, fumaric, malic and citric acid were identified as major organic acids. It was interesting that only fumaric acid content was increased while others decreased during storage. 5. Seventeen kinds of amino acid were identified in the analysis of total amino acid content. Major amino acids were found to be proline, threonine, glutamic acid and lysine. During 12 months storage, 30% of original total amino acid was decreased and this was mostly accounted for free amino acids. 6. Threonine, proline, alanine and valine were identified as free amino acids which showed 85% decrease after 12 months storage. 7. Free sugars of jujube were composed of fructose, glucose and sucrose. They showed 24% decrease after 12 months storage, Especially sucrose was not detected after 4 months storage. 8. HMF of dried jujube was isolated and identified. Its content was increased up to great extent as storage period prolonged. 9. Caffeic, ferulic and P-coumaric acid were identified as jujube phenolic compounds which were significantly decreased during storage. In conclusion, non-enzymatic browning was thought to be more important than enzymatic reaction in the dried jujube. Amino-carbonyl reaction, ascorbic acid oxidation and reaction between phenolic compounds and sugar or nitrogen compounds were observed to be related to this browning.

      • Metabolism of Ginseng Saponin in Animal Body (I)

        이희봉,주충노,Lee, Hee-Bong,Joo, Chung-No 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.2

        인삼(Panax ginseng C.A. Meyer) 뿌리 절편을 효소원으로 사용하여 $^{14}C$-acetate로부터 제조한 방사성 인삼 사포닌을 Thin layer chromatography, High performance liquid chromatography, Autoradiography 등으로 확인하였다. 1 mg의 인삼 사포닌을 쥐에게 구경 투여하고 1시간 후, 간장으로부터의 추출물을 분석한 결과, 회수된 방사능의 약 25%가 분해되지 않은 사포닌 상태로 존재하고 있음을 확인하였다. 또한 인삼 사포닌 투여 (구경)후 시간에 따른 방사능의 변화 양상을 초사한 결과 간에서의 사포닌의 반감기는 4~6시간, 인삼 사포닌의 농도는 $10^{-5}%$ 수준으로 예측되었다. $^{14}C$-labelled ginseng saponins from $^{14}C$-acetate were prepared using the root slices of panaxginseng C.A. Meyer as an enzyme source and the products were demonstrated to be identical with the saponins extracted from the roots by thin layer chromatography, high performace liquid chromatography and autoradiography. The radioactivity of the liver was monitored on time course after the feeding of 1 mg of ginseng saponin containing $^{14}C$-labelled saponin. The extract from the liver of rats killed at one hr. after the oral administration of 1 mg of ginseng saponins containing the $^{14}C$-saponins was analyzed found that 25% of the radioactivity recovered was found as undissociated form of the saponin. It semed that the half life of the saponin might be 4~6 hrs and the concentration of the saponin would be $10^{-5}%$ level in the liver.

      • The Incorporation of Eucaryotic Gene Using Ti Plasmid Vector: I. The Introduction of Yeast Homoserine Dehydrogenase Gene into Agrobacterium tumefaciens

        이희봉,주충노,홍순주,김성완,임창진,김영명,Lee, Hee-Bong,Joo, Chung-No,Hong, Sun-Joo,Kim, Seong-Wan,Lim, Chang-Jin,Kim, Young-Myeong 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.1

        효모에 있어서 threonine과 methionine 생합성에 관여하는 효소들 중 하나인 homoserine dehydrogenase가 식물체내로 도입된 후, 그 역할을 조사하고자 Ti plasmid에서 유도된 pGA658 vector와 homoserine dehydrogenase 유전자 함유 DNA의 재조합을 시도하였다. 그 결과 pGA658 vector의 nos promoter 다음에 효모의 homoserine dehydrogenase 유전자를 함유한 DNA 절편이 방향이 반대로 삽입된 재조합 plasmids가 성공적으로 만들어졌다. 형질전환된 E. coli와 Agrovbacterium tumefaciens는 적절한 항생물질 함유배지에서 저항성을 나타내었으며, 그들의 plasmid를 분리하여 여러가지 제한효소를 이용한 크기를 조사해 보니, 예상되는 크기와 일치하는 것을 관찰할 수 있었다. 또한 형질전환된 E. coli와 A. tumefaciens내에서의 yeast homoserine dehydrogenase 유전자 발현을 조사하기 위해 그 효소의 활성을 조사해 본 결과, 형질전환된 E. coli에서는 대조군에 비해 약간의 증가를 나타내었으나, 형질전환된 A. tumefaciens에서는 변화가 없었다. DNA recombination using plasmid pGA658, a Ti plasmid vector, was tried to study the role of yeast homoserine dehydrogenase after introducing its gene into plant cells. Two recombinant plasmids with opposite orientation of the gene DNA under nos promoter of vector pGA658, pKDB1 and pKDB2, were constructed by the aid of two intermediate vectors, pUC7 and pUC119. Transformed E. coli and Agrobacterium tumefaciens were confirmed by the resistance to appropriate antibiotics and by sizing DNA restriction fragments after plasmid isolation. Assay of homoserine dehydrogenase activity showed that a little increase in its activity was detected in transformed E. coli, but no increase in transformed Agrobacterium tumefaciens, compared with its untransformed strain.

      • Incorportation of Foreign Gene with Ti Plasmid Vector System: (I) Introduction of E. coli Thioredoxin Gene into A. tumefaciens.

        이희봉,주충노,홍순주,김성완,임창진,김영명,Lee, Hee-Bong,Joo, Chung-No,Hong, Soon-Joo,Kim, Seong-Wan,Lim, Chang-Jin,kim, Young-Myeong 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

        외래 유전자를 도입함으로써 식물체에 새로운 유전정보를 부여하려는 노력이 다각적으로 수행되어 왔으며 최근에는 넓은 숙주범위를 갖는 Ti plasmid를 vector로 이용한 외래 유전자의 도입이 활발히 진행되고 있다. 본 연구에서는 Ti plasmid vector의 일종인 pGA658을 이용하여 광합성 조절 등 여러가지 기능을 갖고 있는 것으로 알려져 있는 thioredoxin 유전자를 식물체내로 도입하여 식물체내에서의 그 역할을 조사할 목적으로 DNA 재조합을 시도하였다. E. coli thioredoxin 유전자를 함유하는 pCJF 4의 Hind III-BamHI DNA fragment를 pGA658의 Hind III-Bgl II site에 삽입시키고 E. coli에 transformation 한 후 형질전환체의 확인은 항생물질 marker에 대한 저항성으로, 재조합 DNA의 유전자 지도는 여러가지 제한효소를 이용한 절편의 크기 확인으로, 그리고 도입 유전자의 발현은 효소 활성 측정에 의 하여 확인하였다. 이렇게 확인된 재조합된 plasmid pKDB3를 담배세포로 도입하기 위한 전 단계로서 freeze-thaw 방법으로 Agrobacterium에 transformation 한 후 항생물질 저항성과 제한효소를 이용한 절편의 크기 확인 및 효소 활성 측정에 의하여 pKDB3가 도입되어 안정하게 유지됨을 확인할 수 있었다. In this part of study on the incorporation of foreign gene into plant cells, a derivative of Ti plasmid vector (pGA658), containing E. coli thioredoxin gene, was prepared and introduced into Agrobacterium tumefaciens. A recombinant plasmid, pKDB3, was constructed by transferring HindlII-BamHI DNA fragment of pCJF4, including E. coli thioredoxin gene, into HindIII-BglII restriction sites of plasmid pGA658. By doing this, E. coli thioredoxin gene is expected to express from nos promoter of pGA658 after the incorporation into plant cells. The structure of DNAs isolated from kanamycin-resistant E. coli transformants was convinced by restriction mapping. As a preceding step before incorporation into plant cells, the recombinant plasmid pKDB3 was transformed into A. tumefaciens by freeze-thaw procedure. In Agrobacterium transformants, the expression of E. coli thioredoxin gene was positively observed, and this suggested the stable existence of the E. coli gene.

      • Incorporation of Foreign Gene with Ti Plasmid Vector System: (II) Expression of E. coli Thioredoxin Gene in Cultured Tobacco Cells.

        이희봉,주충노,홍순주,김성완,임창진,김영명,Lee, Hee-Bong,Joo, Chung-No,Hong, Soon-Joo,Kim, Seong-Wan,Lim, Chang-Jin,Kim, Young-Myeong 생화학분자생물학회 1988 한국생화학회지 Vol.21 No.4

        본 연구에서는 E. coli thioredoxin 유전자를 함유하고 있는 재조합된 plasmid pKDB3의 담배세포내로의 도입을 시도하고, 도입된 thioredoxin 유전자의 발현을 조사하였다. 담배(Nicotina tabacum cv Xanthi) 세포로의 재조합 plasmid pKDB3의 도입은 담배잎 절편과 재조합 DNA 및 helper Ti plasmid pTiBo 542를 함유하고 있는 Agrobacterium tumefaciens strain A281과의 cocultivation 방법을 이용하여 행하여졌으며, 형질전환된 담배세포는 항생물질 저항성 배지에서의 callus 형성 여부로 선별되었고, 선별된 형질전환된 calli는 shoot와 root 형성을 위해 적절한 MS agar 배지에서 계속 키워졌다. 이와 같이 형질전환된 담배세포에서 완전한 식물체로 재생된 담배잎에서의 E. coli thioredoxin 유전자의 발현을 조사한 결과 thioredoxin 활성이 형질전환된 담배세포가 정상세포에 비해 9배 정도 더 큰 것으로 나타났다. 이러한 일련의 결과들은 E. coli thioredoxin 유전자가 성공적으로 담배세포에 들어가서 높은 수준으로 발현됨을 보여주고 있다. This study was performed to observe the expression of E. coli thioredoxin gene incorporated in tobacco cells. The recombinant DNA used, pKDB3, had been constructed from a Ti plasmid vector pGA658 and a bacterial plasmid pCJF4 harboring E. coli thioredoxin gene, as described in the preceding paper (Lee et al., 1988). The leaf discs of plant (N. tabacum cv Xanthi) were transformed to kanamycin resistance by the cocultivation with Agrobacterium A281 containing plasmid pKDB3. Transformed leaf discs were cultured in MS agar medium with kanamycin for callus induction. Kanamycin-resistant tobacco calli were continuously grown in MS agar medium for shoot induction, and then in MS agar medium for root induction. Expression of E. coli thioredoxin gene in the plant tissue regenerated from transformed tobacco cells was confirmed by DTNB assay. The thioredoxin activity of transformed tobacco cells was much higher (about 9 times) than that of normal tobacco cells. Our results suggest that E. coli thioredoxin gene was successfully incorporated into tobacco cells, and the incorporated bacterial gene could be expressed at a high level.

      • KCI등재

        안팍구조(構造)-한국건축공간(韓國建築空間)의 구조주의적(構造主義的) 해석(解析)

        이희봉,Lee, Hee-Bong 한국건축역사학회 1992 건축역사연구 Vol.1 No.2

        The binary classification, Yin & Yang, can be shown in our culture. Yin & Yang thinking reveals the Up/Down (Principal/Subordinate) relation in space, and Before/After relation in time, and it shows the sequence of Contrast${\to}$Reconciliation & Unification${\to}$Contrast. For example, a follower is Yin against King, while Yang as a father against his son in our trational cognitive structure. With Left/Right, Front/Rear, and Upper/Lower, In/Out division is a basic body-space term to grasp the space position. In the traditional Korean house, when we go out from the deepest 'In', Anbang to Daechung, we may call Anbang 'In' and Daechung 'Out'. When from Daechung to Anmadang reversely, we call Daechung 'In' and Anmadang 'Out'. Namely, Daechung is both 'In' and 'Out'. This is a fallacy logic in view of western basic conception. 'The In/Out Structure, system of a series of inner spaces, is defined as a transformation of cognitive structure of Yin & Yang thinking to the Korean architectural space. The space structure is one of the important deep structure in Korean society.' The concept of the space structure, apperred in east Asia, can make humane space, for it is not a physical 'type' of typepology but related to cognitive structure of human thinking, The structure is applicable to space design in modern society, for the cognitive structure exists in living culture by transformation.

      • KCI등재

        한국(韓國) 건축역사(建築歷史) 연구의 비판(批判)과 방향모색(方向摸索) - 실천이론(實踐理論)으로서의 건축역사방법(建築歷史方法)-

        이희봉,Lee, Hee-Bong 한국건축역사학회 1992 건축역사연구 Vol.1 No.1

        The purpose of this paper is to suggest new direction to the historical research society of Korean architecture by epistemological discussion. Korean architectural history at present, without question of history for what, for whom, and how, is regarded as 'history for the past' only 'to describe past' or 'to collect correct data' However, history is beyond that; history ought to suggest a direction of present and near future. Moreover, architecture is not pure but applied knowledge, that is, focused to 'how to build'. Therefore, past historical knowledge by research should influence to present design. In order to do that, history of mere data collection should change direction to the history of meaning by interpretation, and then, of finding design principle. The principle used in architectural community becomes theory in scientific research community, and finally is unified to 'theory of practice'. Creative history should be substituted for retrospective history reusing historical reservoir, at the specific situation of Korea; tradition has been discontinued drastically and Western method imported to the point of being culturally-colonized. Some expmples of altanative history are suggested at the end of this paper as conclusion.

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