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감자 (Solanum tuberosum L.) CycD3유전자의 분리 및 특성 분석
강인홍,최승호,이홍근,황현식,이석찬,정태영,임학태,배신철,Kang, In-Hong,Choi, Seung-Ho,Lee, Hong-Geun,Hwang, Hyun-Sik,Lee, Suk-Chan,Jung, Tae-Young,Lim, Hak-Tae,Bae, Shin-Chul 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.4
D-type cyclins are believed to regulate the G1 to S phase transition in response to nutrient and hormonal signals. We investigated the expression characteristics of the key cell-cycle regulators, mitotic and G1 cyclins in potato (Solanum tuberosum L.). We isolated D-type cyclin gene from potato and it was classified as D3 cyclin by sequence similarities and a phylogenetic analysis, and named as StcycD3;1. The accumulation of transcripts was predominantly associated with mitotically active organs, such as stolons, roots, flowers, leaves, and stems. Transcription of StcycD3;1 can be induced by sucrose. D-type cyclin은 호르몬과 영양분의 영향을 받아 세포주기의 G1기에서 S기로 전환을 조절하는 인자이다. 우리는 감자에서 이 유전자를 분리 해냈고, 염기서열 분석을 통하여 D3 cyclin으로 분류하였다. 그리고 StCycD3;1이라 명명하였다. 다른 D cyclin유전자가 세포 분열이 활발한 조직에서 발현되는 것과 같이 StCycD3;1은 감자의 괴경, 뿌리, 꽃, 잎, 줄기, 뿌리줄기, 복지에서 다양한 발현 양상을 보였고, 영양분의 하나인 sucrose에 의하여 발현이 유도되는 것을 확인하였다.
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조일형 ( Jo Il Hyeong ),박재홍 ( Park Jae Hong ),김영규 ( Kim Yeong Gyu ),이홍근 ( Lee Hong Geun ) 한국물환경학회 2003 한국물환경학회지 Vol.19 No.3
In order to treat the dyeing wastewater, the UV/TiO₂/H₂O₂ system was investigated, and proper protreatment methods were examined to reduce the load on the system considering economical and techical efficiency. Also, to obtain the maximize the removal efficiency of dyeing wastewater, optimal operating conditions for the photocatalytic degradation were investigated by using the responsed surface methodology(RSM) . The independent variables were TiO₂ dosage, H₂O₂ concentration and removal efficiency of dyeing wastewater was dependent variable. With the 2²-factional factorial experimental design, 11 experiments were carried out at various condition, with 4 factorial points, 3 center points and 4 axial points. Maximum removal efficiency(85%) of dyeing wastewater was obtained at TiO₂ dosage(1.82g/L), H₂O₂ concentration (980㎎/L) for photocatalytic oxidation reaction(20min).