PC 카메라를 이용한 수돗물의 과망간산칼륨소비량 적정 자동화

이형춘,Lee, Hyeong-Choon 한국환경보건학회 2008 한국환경보건학회지 Vol.34 No.1

An automatic titration system using a PC-camera with a color filter on its lens was used in the $KMnO_4$ consumption test of tap water and distilled water in relation to blank tests. The very faint pink color of titration end point could be effectively detected by using a yellow cellophane paper as a color filter. The average hue value (Havg) of 192 pixels in the image of the sample solution being titrated was computed and followed up at regular time intervals during titration in order to detect the titration end point. The Havg decrease of 2 degrees from the average of first 10 Havgs was regarded as reaching the end point. The volume of 0.01N $KMnO_4$ consumed by a tap water sample was $0.728{\pm}0.022ml$ in manual titration and $0.735{\pm}0.013ml$ in automatic titration (p=0.580). The volume of 0.01N $KMnO_4$ consumed by a distilled water sample was $0.383{\pm}0.015ml$ in manual titration and $0.367{\pm}0.015ml$ in automatic titration (p=0.252). The high p-values for t-test suggested that there were good agreements between manual and automatic titration data and the automatic method proposed in this article was considered to effectively replace the manual titration.

전분분해효소와 유산균에 의한 보리의 유산발효

이형춘,구영조,신동화 한국식품영양학회 1988 韓國食品營養學會誌 Vol.1 No.2

효소로써는 태평양화학주식회사에 액화아밀라아제를, 유산균으로써는 Lactobacillus sp. L-5를 선발하여, 이들을 고체상태의 찐 보리기질에 동시에 접종한 후 37℃로 48시간 배양한 결과 풍미가 우수한 보리의 유산발효산물이 얻어졌다. 또한, 보리기질의 수분함량을 70%내외로 할 경우 발효산물의 물성이 우수하였다. 회전드럼형발효조를 사용하여 37℃, 15rpm의 조건하에서 발효할 경우, 유산균은 대수증식기에서 0.6hr^-1의 비증식속도로 증식하여 배양 12시간만에 정체기로 들어갔으며, 산도는 계속 증가하여 배양 48시간후에 1.17%였다. 발효경과에 따라 보리맛은 계속 감소하여 48시간 배양후에는 요구르트적 풍미를 갖는 호상(糊狀)의 발효산물로 되었다. Fermented barley food was produced by the combining action of an enzyme and a lactobacillus. When Lactobacillus sp. L-5 and commercial liquefying amylase from Tae Pyeong Yang Chemical Co. were selected, inoculated on steamed barley and cultivated at 37℃ for 48hrs, the fermented product of good quality was obtained. In batch cultivation using rotary drum fermentor, viable cell count reached 1.1×10 exp (9)CFU/g after 12hrs' cultivation, and specific growth rate in logarithmic phase was 0.6hr^-1. Viable cell count, acidity, pH, concentration of reducing sugar and viscosity of the 48hrs' fermentation product from rotary drum fermentor was 4.3×10 exp (8)CFU/g. 1.17%, 3.1, 10.7% and 1430cp.

[12월의 주제 : “탄생, 그 기쁨의 무게”] 만들고 또 만들고…

이형춘 새가정사 2009 새가정 Vol.- No.-

유가배양에 의한 알콜증류폐액으로부터 빵효모의 생산+

이형춘 서원대학교 응용과학연구소 1996 응용과학연구 Vol.5 No.1

Nutrients including carbon source were supplied to an alcohol distillery wastewaster used as fermentation media for baker's yeast, production in order to improve productivity of cultivation. Glucose was used as carbon source, and it coud be maintained at a sufficiently low concentration and completely removed at the end of cultivation by a computer-controlled feeding system using DO as a feeding indicater. In the batch culture without supplying any nutrients, cell density was 2.08g/l, but high cell density of 21g/l was obtained in fed-btach culture by supplying carbon, nitrogen and phosphorus source. In fed batch culture using ammonia water as both pH control reagents and nitrogen source, cell density of 12g/l was obtained by a simple cultivation method and cell yield was 0.44g dry cell/g consumed glucose.

곰팡이, 효모 및 유산균에 의한 보리의 혼합발효

이형춘,구영조,신동화 한국식품영양학회 1988 韓國食品營養學會誌 Vol.1 No.2

곰팡이로써는 Rhizopus delemar IFO 4746, 효모로써는 Hansenula anomala IFO 0568, 유산균으로써는 Lactobacillus sp.를 선발하여 이들을 고체상태의 찐 보리기질에 동시에 접종한 후 30℃로 2일, 37℃로 3일 혼합배양하여 풍미가 우수한 보리발효산물을 얻었다. 발효시 시간경과에 따라 pH는 24시간만에 급격히 떨어져 배양 5일후에는 3.4였고, 산도는 계속 증가하여 5일후에는 1.9%였으며, 색도는 계속 감소하였다. Fermented barley food was produced by the mixed culture fermentation with a tri-culture system of a mold, a yeast and a lactobacillus. When Rhizopus delemar IFO 4746, Hansenular anomala IFO 0568 and Lactobacillus sp. L-5 were selected and cultivated on steamed barley at 30℃ for 2days and 37℃ for 3days, the fermented product of good quality was obtained. During fermentation, changes in acidity, pH, water content and color of fermented barley were examined.

마이크로컴퓨터에 의한 溫熱指數의 自動測定 및 學習

이형춘,우세홍,김을환,최한영,유영식,김남천,이상건 서울保健大學 1991 論文集 Vol.11 No.1

알콜증류폐액을 이용한 빵효모의 유가배양에 있어서 온-라인 FIA에 의한 암모늄농도의 제어

이형춘 서원대학교 응용과학연구소 1997 응용과학연구 Vol.6 No.1

Ammonium was maintained at the relatively low range of concentration in an alcohol distillery wastewater used as fermentation media of Baker's yeast that was produced by fed - batch culture in which glucose was supplied by the increase of DO of medium. On-line FIA system and a program for the automatic analysis and control of ammonium were developed and ammonium concentration of wastewater media could be maintained within the range of 7.0 ~ 27.7mM. Ammonium control not only enabled economic supply of nitrogen source but also increased the specific growth rate and biomass yield (Yx/s).

한지자숙폐액 희석액을 이용한 사료첨가효모의 배양에서 자숙원액을 영양성분의 첨가에 의한 균체농도의 증가

이형춘 서원대학교 응용과학연구소 1999 응용과학연구 Vol.8 No.1

The undiluted Korean paper digestion wastewater and some major nutrients were added during the cultivations of a feed additive yeast in the diluted wastewater media (wastewater:distilled water=1:6.5) in order to increase the cell concentration. The addition of undiluted wastewater neary reaching the stationary phase increased the maximum cell concentration by 2.7 times higher than no addition. And the addition of undiluted wastewater before reaching the stationary phase increased the maximum cell concentration by only 2.0 times, but the cultivation time to maximum cell concentration was thought to be shortened. The addition of glucose as a carbon source, (NH())()SO() as a nitrogen source and KH()PO() as a phosphorus source to the wastewater media could increase the maximum cell concentration by 4.2 times. by adding (NH())()SO() and KH()PO() together with the undiluted wastewater the maximum cell concentration could be obtained faster than by adding only undiluted wastewater.

휘발성염기질소의 자동분석

이형춘 서원대학교 응용과학연구소 2003 응용과학연구 Vol.12 No.1

돈육과 우육의 휘발성 염기질소 분석에 기존의 미량확산법 대신 더 빠르고 더 간편한 자동화 FIA 장치를 이용한 방법을 사용할 수 있는가를 통계적으로 검토하였다. 동일한 시료에 대하여 미량확산법과 자동화 FIA장치를 이용한 방법으로 분석한 data의 상관계수는 돈육의 경우 0.993이고 우육의 경우 0.996으로서 상관성이 매우 컸다. 또한, 적합도 검정의 결과 99.5%의 유의 수준에서 조차도 두가지 방법의 유의차가 인정되지 않을 정도로 두가지 방법의 결과가 서로 잘 일치하였다. 따라서, 돈육과 우육의 휘발성 염기질소 분석에 자동화 FIA장치에 의한 방법이 사용될 수 있다고 생각되었으며, 다른 식육류에도 적용될 수 있다고 생각되었다. An automated method using FLA apparatus with an ammonia electrode as a detector was statistically investigated to be able to replace traditional Conway method in the analysis of volatile basic nitrogen in pork and beef samples. The correlation coefficient between the data from Conway method and the automated FLA method was 0.993 for pork samples and 0.996 for beef samples. No significant difference between two methods was recognized even at the significant level of 99.5% in Chi-sqaure tests. The good agreement between the data from tow methods showed that the automated FLA method which is faster and more convenient than Conway method can be applied in the analysis of volatile basic nitrogen in various meats.

알콜증류폐액을 이용한 빵효모배양에서 포도당, 암모늄 및 인산의 자동첨가에 의한 증균 : 온라인 FIA시스템에 의한 인산의 자동분석 및 제어 Automatic Analysis and Control of Phosphate with an On-line Flow Injection Analysis System

이형춘 서원대학교 응용과학연구소 2002 응용과학연구 Vol.11 No.1

알콜증류폐액에 빵효모를 배양하는 중에 포도당, 암모늄 및 인산을 자동첨가하여 균체량을 효율적으로 증가시킴으로써 폐액의 재이용성을 향상시키고자 하였다. 포도당은 DO를 제어파라미터로 하는 제어방법을 사용하여 자동첨가하였으며, 암모늄 pH-stat 방법을 사용하여 HN_4OH 로서 자동첨가하였다. 인산의 경우에는 분석방법으로써 ascorbic acid법을 채택한 on-line FIA system을 제작하여 사용하였다. 이 system으로 배양 19.4시간후부터 배양액을 자동분석하고 제어한 결과 배양액의 PH_4P 농도를 23.3~43.4mg/L의 낮은 농보범위로 유지할 수 있었다. 세가지 영양성분을 자동처가하면서 배양한 결과 증식의 정체없이 규체량을 33.0배 더 증가시킬 수 있었다. 배양전기간의 비증식속도는 0.19hr^-1였다. Automatic addition of glucose , ammonium and phosphate to an alcohol distillery wastewater and the control of them at low concentration were tried to efficiently increase the cell concentration of a baker's yeast cultivated in that wastewater. Glucose was added by a method which uses DO as control parameter. Ammonium was added by a pH-stat method. For the automatic addition of phosphate, an on-line FIA system which adopted ascorbic acid method was developed for the automatic analysis of phosphate. With this system, the PO_4-P concentration of fermentation media was automatically analyzed and controlled from after 19.4hr in the low range of 23.3∼43.4 ㎎/L. The cell concentration could be efficiently increased by 33.0 times after the addition of three nutrients. Overall specific growth rate was 0.19hr^-1