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Retroviral vector를 이용한 종양괴사인자 (TNF-$\alpha$) 유전자 이입 암세포에서 종양괴사인자 수용체의 발현
이혁표,유철규,김영환,심영수,한성구,Lee, Hyuk-Pyo,Yoo, Chul-Gyu,Kim, Young-Whan,Shim, Young-Soo,Han, Sung-Koo 대한결핵및호흡기학회 1997 Tuberculosis and Respiratory Diseases Vol.44 No.6
연구배경 : 종양괴사인자(tumor necrosis factor ; TNF)는 다양한 생물학적 기능을 가지고 있는 바, 그 중 생체 외에서 증명된 뚜렷한 항암 효과로 말미암아 최근 항암 유전자요법의 중요한 대상으로 관심을 모으고 있다. 현재 유전자 이입의 기술적 문제로 생체 외에서 암세포에 유전자 이입을 시행한 후 이를 다시 환자의 생체내로 이식하는 방법이 연구의 주종을 이루고 있다. 그러나 저자들의 과거의 연구를 포함한 여러 연구에서 TNF가 이입된 암세포는 TNF에 대해 내성을 보이는 것으로 증명되었고 이에는 새로이 방어 단백질을 합성하는 것이 관여할 것이라는 시사가 있었다. 이 획득내성의 기전을 밝히는 것이 종양생물학의 이해를 넓히고 보다 효과적인 항암 유전자요법을 개발하기위한 매우 중요한 과제로 생각된다. Background : Tumor necrosis factor(TNF) has been considered as an important candidate for cancer gene therapy based on its potent anti-tumor activity. However, since the efficiency of current techniques of gene transfer is not satisfactory, the majority of current protocols is aiming the in vitro gene transfer to cancer cells and re-introducing genetically modified cancer cells to host. In the previous study, it was shown that TNF-sensitive cancer cells transfected with TNF-$\alpha$ cDNA would become highly resistant to TNF, and the probability was shown that the acquired resistance to TNF might be associated with synthesis of some protective protein. Understanding the mechanisms of TNF-resistance in TNF-$\alpha$ cDNA transfected cancer cells would be an important step for improving the efficacy of cancer gene therapy as well as for better understandings of tumor biology. This study was designed to evaluate whether the levels of TNF receptor mRNA expression and soluble TNF receptor release from cancer cells are changed after TNF-$\alpha$ cDNA transfection. Method : We transfected TNF-$\alpha$ c-DNA to WEHI164(murine fibrosarcoma cell line), NCI-H2058(human mesothelioma cell line), A549(human non-small cell lung cancer cell line), ME180(human cervix cancer cell line) cells using retroviral vector(pLT12SN(TNF)) and confirm the expression of TNF with PCR, EUSA, MTT assay. Then we determined the TNF resistance of TNF-$\alpha$ cDNA transfected cells(WEHI164-TNF, NCIH2058-TNF, A549-TNF, ME180-TNF) and evaluated the TNF receptor mRNA expression with Northern blot analysis and soluble TNF receptor release with EUSA. Results : The TNF receptor mRNA expressions of parental cells and genetically modified cells were not significantly different. The soluble TNF receptor levels of media from genetically modified cells were lower than those from parental cells. Conclusion : The acquired resistance to TNF after TNF-$\alpha$ cDNA transfection may not be associated with the change in the TNF receptor and the soluble TNF receptor expression.
후천성면역결핍증후군 환자에게 급성 호산구성 폐렴 양상을 보인 폐포자충 폐렴
이보라 ( Bo Ra Lee ),김현국 ( Hyun Kook Kim ),박이내 ( I Nae Park ),최상봉 ( Sang Bong Choi ),정훈 ( Hoon Jung ),이현경 ( Hyun Kyung Lee ),이성순 ( Sung Soon Lee ),이영민 ( Young Min Lee ),이혁표 ( Hyuk Pyo Lee ),최수전 ( Soo Jeo 대한결핵 및 호흡기학회 2010 Tuberculosis and Respiratory Diseases Vol.68 No.2
A 73-year-old man was admitted with a sudden onset of dyspnea. He had never smoked. The chest radiograph and computed tomography revealed bilateral ground glass opacity and an enlarging perihilar consolidation with lymphadenopathies. There was a higher percentage of eosinophils (72%) in the bronchoalveolar lavage fluid (BALF) than normal. The patient was diagnosed with acute eosinophilic pneumonia and managed with steroid. Pneumocystis pneumonia (PCP) was diagnosed by an examination of the BALF, and the patient was treated with trimethoprim-sulphamethoxazole. The patient tested positive to the HIV antibody and the peripheral blood CD-4 positive lymphocyte count was only 33/μL. The percentage of eosinophils in the BALF can increase in some cases of PCP that is complicated with AIDS. Only a few cases of eosinophilic pneumonia associated with PCP pneumonia have been reported in patients with AIDS but there are no case reports in Korea. This case highlights the need to consider PCP when the percentage of eosinophils in the BALF is elevated.
폐색전증과 파종성혈관내응고가 합병된 특발성 과호산구 증후군 1례
이종성 ( Jong Sung Lee ),이현경 ( Hyun Kyung Lee ),이성순 ( Sung Soon Lee ),진재용 ( Jae Yong Jin ),이영민 ( Young Min Lee ),이혁표 ( Hyuk Pyo Lee ),김주인 ( Joo In Kim ),구본일 ( Bon Il Ku ),최수전 ( Soo Jeon Choi ),염호기 ( Ho K 대한결핵 및 호흡기학회 2004 Tuberculosis and Respiratory Diseases Vol.57 No.6
조주연 ( Joo Yeon Cho ),이현경 ( Hyun Kyung Lee ),이성순 ( Sung Soon Lee ),이혜경 ( Hye Kyung Lee ),이영민 ( Young Min Lee ),이혁표 ( Hyuk Pyo Lee ),김주인 ( Joo In Kim ),최수전 ( Soo Jeon Choi ),염호기 ( Ho Kee Yum ) 대한결핵 및 호흡기학회 2006 Tuberculosis and Respiratory Diseases Vol.61 No.5
Acute fibrinous and organizing pneumonia (AFOP) is a histological pattern consisting of prominent intra-alveolar fibrin and organizing pneumonia, with out hyaline membranes or prominent eosinophilia. The clinical manifestations of AFOP resemble those of acute lung injury such as acute interstitial pneumonia (AIP). However, the classic histological patterns of AFOP differ from diffuse alveolar damage (DAD), bronchiolitis obliterans with organizing pneumonia (BOOP) or acute eosinophilic pneumonia (AEP). The characteristic intra-alveolar fibrin ball and lack of classic hyaline membrane are the predominant histological features of AFOP. Although some reports suggest that its clinical course is less catastrophic than DAD, the clinical entity that distinguishes AFOP from DAD has not been established. We present a case of pathologically demonstrated AFOP in a 79-year-old man. The radiological findings of our case were similar to those of DAD, presented with diffuse bilateral lung infiltrations. However, despite the rapid development of respiratory failure, the patient had a better response and outcome to steroid therapy than what would be expected for DAD. (Tuberc Respir Dis 2006; 61: 479-483)
기관지내시경 검사상 우연히 발견된 무증상의 기관지 과립세포종 2예
강은희,이성홍,나영숙,최태영,남궁준,정병오,이혁표,김주인,염호기,최수전,조혜제,이혜경,Kang, Eun-Hee,Lee, Sung-Hong,Na, Young-Sook,Choi, Tae-Young,NamGung, June,Jeoung, Byung-Oh,Lee, Hyuk-Pyo,Kim, Joo-In,Yum, Ho-Kee,Choi, Soo-Jeon,Cho, Hye 대한결핵및호흡기학회 1999 Tuberculosis and Respiratory Diseases Vol.46 No.1
저자들은 타질환으로 입원중 기관지내시경검사상 우연히 발견된 과립세포종 2예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Granular cell tumor(formerly named to be granular cell myoblastoma) was first described by Abrikossoff in 1926 and is a rare tumor thought to be of Schwann cell origin. It can occur at any soft tissue of the body, but most cases are found at the tongue, skin, breast and GI tract Only 6% of them occur in respiratory system. We report two cases of asymptomatic granular cell tumor of the bronchus that were detected incidentally by bronchoscopy. One patient had aspiration pneumonia, the other had immotile cilia syndrome. The former patient was simply observed and bronchoscopic extirpation of the tumor was done in the latter patient.