울산지역 곤충군집에 관한 생태학적 연구

이해풍,강인구 ( Hai Poong Lee,In Koo Kang ) 한국환경생물학회 1986 환경생물 : 환경생물학회지 Vol.4 No.1

Present studies were carried out to determined the structure of Arthropod`s community and their size of 3 sites in Ulsan city, Kyungsangnam-Do. The results obtained were ; 1) Numeral size of Arthropods in the industrial district is comparatively smaller than that in the nonindustrial district where are not polluted so much. 2) Despite of the different composition depending on surveyed area, the insects in order Orthoptera, Hymenoptera, Hemiptera, Coleptera, Collembola and Araneina form the majority part in the surveyed area. 3) Atractomorpha bedeli in Orthoptera, Paratrechina flavipes in Hymenoptera, Cryptocephalus approximates in Coleoptera and Tomocerus varius in Collembola are the common species in the surveyed area. 4) The community of Orthopterous, Hemipterous, Collembolous insects are comparatively higher in nonindustrial area than in industrial area. However, the community of Coleopterous insects is comparatively higher in industrial area than in nonindustrial area.

연교패독산가미방(連翹敗毒散可味方)이 알러지성 접촉피부염에 미치는 영향(影響) - DNCB 재노출부위의 생쥐 피부손상완화와 비만세포 분포 변화를 중심으로 -

권오성,김진택,박인식,안상현,이해풍,김호현,강윤호,Kwon, Oh-Sung,Kim, Jin-Tack,Park, In-Sick,Ahn, Sang-Hyun,Lee, Hai-Poong,Kim, Ho-Hyun,Gang, Yun-Ho 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 연교패독산가미방(連翹敗毒散加味方)이 알러지성 접촉피부염 유발시 발생되는 면역과민반응과 염증반응 저해에 미치는 영향을 조사하기위해 행해진 것으로 2,4-dinitrochlorobenzene(DNCB, 5% DNCB $25{\mu}{\ell}$로 감작, 7일경과 후 2.5% DNCB $5{\mu}{\ell}$로 유발)처리로 알러지성 접촉피부염이 유발된 ICR계 암컷 생쥐에 연교패독산가미방추출물(連翹敗毒散加味方抽出物(0.51g/kg/day)을 음용투여한 다음 contract hypersensitivity assay, 피부의 일반적인 구조 그리고 비만세포의 분포 변화를 관찰하였다. Contract hypersensitivity assay의 결과, 연교패독산가미방추출물(連翹敗毒散加味方抽出物) 투여군의 ear swelling이 DNCB군에 비해 유의성있게 감소한 것으로 나타났다. 알러지성 접촉피부염 유발 후 관찰되는 피부손상은 48시간에 제일 심한 것으로 관찰되었으나, 연교패독산가미방추출물(連翹敗毒散加味方抽出物) 투여군에서는 DNCB군 비해 완화된 결과를 보였다. 연교패독산가미방추출물(連翹敗毒散加味方抽出物) 투여군의 진피에서 림프구를 비롯한 염증관여세포의 이주와 혈관생성 그리고 표피내로의 림프구의 침윤 등이 감소되었고, 표피 기저층과 가시층세포에서는 세포질내 액포화와 세포사이 공간 확장도 줄어든 것으로 관찰되었다. 한편 알러지성 접촉피부염 유발 후 분포가 증가한 비만세포는 형태가 주로 세포내 함유물이 분비된 degranulated type로 나타났다. 이에 반해 연교패독산가미방추출물(連翹敗毒散加味方抽出物) 투여군는 DNCB군에 비해 출현 비만세포 수가 일부 감소했고, 비만세포의 형태는 granulated type였다. 또한 serotonin 면역조직화학적 염색결과로 연교패독산가미방추출물(連翹敗毒散加味方抽出物) 투여군에서 se-rotonin 양성반응성이 높은 granulated type의 비만세포가 주로 관찰되었다. 이상의 결과로 미루어 연교패독산가미방추출물(連翹敗毒散加味方抽出物)은 알러지성 접촉피부염으로 유발된 피부 손상과 염증을 완화시키는 효과가 있는 것으로 사료된다. After allergic contact dermatitis elicitated by Dinitrochlorobenzene(DNCB) treatment, ICR female mice administered Yunkyopaedocksangamibang(YPGM) extract were observed to investigate the effect of YPGM on allergic contact dermatitis. This study investigated that contact hypersensitivity assay, abdominal skin morphologic changes including mast cells. At contact hypersensitivity assay, the right ear swelling in YPGM group were probability decreased than DNCB group. At observation of abdominal skin morphologic change, the infiltration of lymphocyte, lymphocyte insertion to epithelium, enlarged capillary, angiogenesis, and damages of epithelium as cytoplasmic vacuolation and enlarge of inter cellular space in YPGM were diminished than DNCB group. The number of mast cell was increased both DNCB and YPGM group. The shape of mast cell in DNCB group was mainly appeared degranulated type, but granulated type in YPGM group. The number of serotonin positive cell was increased both DNCB and YPGM group. The shape of serotonin positive cell in DNCB group was mainly appeared degranulated type, but granulated type in YPGM group. As results indicated that the YPGM extract administration work on the mitigation of skin damages in mouse with allergic contact dermatitis.

호주산 독거미 ( 꼬마거미과 : 독거미속 ) 에 관한 연구

김주필(Joo Pil Kim),이해풍(Hai Poong Lee) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.3

N/A The Red-Back Spiders(Latrodectus hasselti) were found in all parts of Australia except in the hottest deserts and on the coldest mountains. The spider is now generally recognized as being the same family as the Katipo Spider of New Zealand and the Black Widow Spider of America. This species is the only representative of the genus Latrodectus in Australia. The Red-Back Spider was probably the most common cause of serious spider bites between 1927, when records were first kept, and 1956, when an anti=venom became available. The authors redescribe and figured detailly Red-Back Spider, L. hasselti, which was collected during a field trip of Australia.

혈부축어탕이 Triton WR-1339에 의한 고지혈증 유발 생쥐 간조직내 지질 축적 억제에 미치는 영향

김호현,방혜정,강윤호,박인식,안상현,김진택,이해풍,Kim, Ho-Hyun,Bang, Hyui-Jeng,Gang, Yun-Ho,Park, In-Sick,Ahn, Sang-Hyun,Kim, Jin-Tack,Lee, Hai-Poong 대한동의생리학회 1999 동의생리학회지 Vol.14 No.2

After Triton WR-1339 (TX; 600mg/kg) intraperitoneal injection, hepatic tissues of ICR mice were intragastric injected with Hyulboochucketang extract(HCE; 3.3ml/kg/day) were observed to investigate the suppressive effect of lipid accumulation that evoke by the antihyperlipidemic effect of HCE. These hepatic tissues were fixed in fromol-calcium solution and were cryocut. These tissues stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone(PAN) method for cholesterol. After TX treatment, the increase of hepatocyte having meshlike cytoplasm(HHMC) were shown in all hepatic lobules and the hepatic plates were disappeared in the aggregative region of HHMC. The number of blue black colored lipid drop and dark green colored asterisk shaped cholesterol particle in hepatic cytoplasm were increased and the size of lipid drop and cholesterol particle were enlarged. But, in HCE-treated mice, the HHCM were disappeared and hapatic plate were rearranged. The number of lipid drop and cholesterol particle were decreased than TX-treated mice and the size of lipid drop and cholesterol particle were diminished. As results indicated that the HCE work on the suppression of lipid accumulation in hepatic tissue of hyperlipidemic mice caused by disturbance of lipid metabolism.

산림내 지표면에 서식하는 딱정벌레류(Coleoptera : Carabidae)의 종구성과 계절적 변동에 관한 연구

이해풍,이건영 고려대학교 한국곤충연구소 1995 昆蟲硏究誌 Vol.21 No.1

산림내 지표면에 서식하는딱정벌레류(Coleoptera : Carabidae)의 종구성과 계절적 변동에 관한 연구. 이해풍, 이건형(동국대학교 응용생물학과 본 연구는 1985년부터 1987년까지 경기도 남양주군에 위치한 예봉산 내에서 세가지 다른 수림내 지표면에서 Pitfall trap을 이용하여 딱정벌레류의 종구성과 계절적 변동을 이해하고저 실시하였다. 채집된 딱정벌레는 총 4,148마리로서 35종으로 구성되었다. 주요 우점종은 6종으로서 Carabus sternburgi Roechke, Damaster jankowski Oberthur, Synuchus nitidus (Motschulsky), S. cyclroderus (Bates), S. agonus (Tschitscherine), Chlaenius naeviger Morawitz 등 이었다. 서로 다른 수림간에 우점종의 구성과 풍부도가 현격히 차이가 있었다. 그러한 차이점은 지표면의 환경, 특히 낙엽층, 부식층, 토양습도 등의 차이에 의한 것으로 사료되었다. 전 조사기간 중 딱정벌레류는 연중 3회의 peak 시기를 나타내었으며 그 기간은 5월, 8월, 10월이었다. 우점종들의 발생량과 우점률은 시기별로 차이를 뚜렷이 나타내었는데 C. sternburgi는 5월에 가장 많이 채집되었고, D. jankowski는 8월에, S. nitidus와 S. cycloderus는 10월중에 최성기를 보였다. 종다양도 지수인 Shannon-Wiener 지수는 잣나무숲에서 가장 높았으며 다음은 활엽수림, 적송림 등의 순서였다. 본 보고서를 통하여 종다용도지수의 이용과 실용성에 대하여 논의 하였다. The species composition and seasonal abundance of ground beetles (Coleoptera: Carabidae) was studied in three different tree stands in Mt. Yebong-san, Kyonggi-do, Korea, from 1985 to 1987. Pitfall traps were used to sample adult carabids. During the study, the total 4148 carabids composed 35 species were collected. There were six dominant species: Carabus sternbergi Roechke, Damaster jankowskii Oberthur, Synuchus nitidus(Motschulsky), S. cycloderus (Bates), S. agonus (Tschitseherine), Chlaenius naeviger Morawitz. The species composition and abundance of the dominant species varied with different tree stand. We suspected that differences in the ground condition among habitats were the main factors influencing the distribution and species diversity of carabids. Carabids had three peak time a year during the study period : in May, August and October. The abundance and dominance of six major species varied with season ; C. sternberggi was the moat abundant species in May; D. Jankowskii dominated in August ; and S. nitidus and S. cycloderus were the predominant species in October. Shannon-Wiener Index was the greatest in pinenut tree site und followed by deciduous tree site and Korean pine tree site. The use of diversity indices was discussed.

혈부축어탕의 고지혈증 유발 생쥐 신장내 지방 축적 감소효과

김호현,강윤호,방혜정,김진택,박인식,안상현,이해풍 世明대학교 한의학연구소 1998 韓醫學硏究所 論文集 Vol.1 No.-

Renal tissues of ICR mouse were intraperitoneally injected with Hyulboochucketang(HCE;1.28g/kg/day) after Triton WR-1339(TX;600mg/kg) injection were observed to investigate the lipid accumulation suppressive effect of HCE. The renal tissues were obtained at hour 24,48 and 72 after TX injection that were fixed in fromol-calcium solution and were cryocut. The tissue stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone method for cholesterol. The parietal layer of Bowman's capsule is swelled as cuboidal type at hour 48 after TX injection and the lipid blot and cholesterol particle were noticeably increased in glomerulus than control group. But the parietal layer of Bowman's capsule in HCE treated group was shaped as squamous type that was looked like as normal morphology and the lipid blot and cholesterol particle w3ere considerably decreased in glomerulus than TX group. As results indicated that the accumulation of lipid including cholesterol caused by TX injection were mitigated in renal tissues by the antihyperlipidermic effect of HCE.

산림토양내의 병, 해충방제를 위한 생태학적 기초연구 : I.소나무림과 활엽수림내의 지표곤충 1.Soil Surface Insects in Pine Plantation and Oak Forest

이해풍,이건형,김상훈 東國大學校 1989 論文集 Vol.28 No.-

본 연구는 경기도 남양주군 조안면 진중리 소재 동국대학교 연습림의 소나무림과 참나무림 내에서 1987년 5월부터 11월까지 실시되었으며 지표토양곤충군집을 대상으로 종의 구성, 먹이식습성의 구성, 우점종의 구성, 균등도 등이 조사되었다. 조사된 종은 소나무림 내에서 41과 94속 128종으로 확인되었으며 종의 수가 많은 것으로는 딱정벌레목, 벌목, 톡톡히목 그리고 파리목이었고 참나무림의 경우는 46과 95속 135종이었으며 딱정벌레목, 벌목, 톡톡히목 그리고 파리목 순으로 종수가 많았다. 식습성에 따른 영양관계구성은 초식성의 것이 두 지역 모두 가장 많아 소나무팀에서는 전체 절지동물중 43.6%이었고 다음으로 검류를 포함한 포식충류가 37.2% 그리고 잡식성인 것이 19.0%이었다. 한편 참나무림에서는 각각 36.8%, 47.8% 그리고 15.3%를 나타내어 전체적으로는 종의 다양도에서 소나무림이 약간 높았을 뿐 뚜렷한 차이를 볼 수 없었다. 계절별 군집의 변화동태에서는 두 지역간 약간의 차이를 보여서 포식자의 발생밀도가 참나무림에서 9월 중순에 대조적으로 높았을 뿐 큰 차이없이 전체적으로 5-7월에 높았고 9-10월에 낮았다. During the period from May, 1987, to November, 1987, (on the basis of pitfall trap capture,) 128 species and 135 species of insects together with unidentified were collected in soil surface of Pine plantation and Oak forest respectively in Kyounggi-Do. Spiders were most abundant species group, comprising 22.6% of the total number of individuals in Pine plantation. the most abundant insect groups were Coleoptera, Hymenoptera, Collembola and Diptera in Pine plantation, and Coleopteram Hymenoptera and Diptera in Oak forest. Oak forest supported more species than Pine plantation which supported only 4 species at very high population levels and the rest at low levels. Many of the species in two study areas were either general plant feeders or predators. Seasonal abundance of the total insects including spiders was high in the periods of May to July and low in Nomber.

아황산가스가 집파리의 산란시기와 우화율에 미치는 영향

이해풍,이복임,최덕일 동국대학교 산업기술환경대학원 1994 산업기술논총 Vol.2 No.-

When Musca domestica L. was exposed to the different levels of SO_2(0.3, 0.4, 1.5 ppm) in an air pollutant fumigation chamber, in the case of 0.4 ppm exposed, their oviposition rate significantly decreased with the increasing exposure period at 0.4 ppm and their oviposition was also delayed. The number of pupae from the house fly exposed to SO_2 distinctly decreased when compared with control group and this trend was more pronounced at F_2 than at F_1 generation. The emergence of pupae from the cohorts exposed to SO_2 showed a distinctly decreasing trend with the increase in exposure period and in the concentration of SO_2.

긴볼레기말의 항고지혈증 효과에 관한 연구 : Triton WR-1339 주사에 의한 고지혈증 유발 생쥐의 간세포내 지방 축적 감소를 중심으로 Based on the Decreasing Effect of Lipid Accumulation in Hepatocyte of Murine with Hyperlipidemia induced by Triton WR-1339

박인식,안상현,정재만,강윤호,이해풍,서귀문,홍용기,김호현,김진택 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.7 No.2

본 연구는 긴볼레기말 추출물의 항고지혈증 효과를 조사하기 위해 ICR 생쥐에 TritonWR-1339(TX) 복강주사로 인위적인 고지혈증을 유발시킨 후 긴볼레기말 추출물(30㎎/㎏)를 복강주사하여 시간의 경과에 따른 간세포내에서의 지방 축적 변화를 조직화학적으로 관찰하였다. TX 주사후 그물구조의 세포질출 가진 간세포가 간엽 전체에서 관찰되었고, 일부 간소엽에서는 간세포 손상으로 인한 간세포판 소실이 나타났다. 또한 간세포내 지방축척도 증가하여 전체 간소엽의 간세포에서 지방의 과출현을 확인 할 수 있었고, 지방의 크기도 대조군에 비해 증가된 것으로 관찰되었다. 그러나 긴볼레기말 추출물 주사군에서는 그물구조의 세포질을 가진 간세포의 수가 TX 주사군에 비해 감소되었고, 대부분의 간소엽에서 정상적인 간세포판의 배열을 확인할 수 있었다. 간세포내의 지방 축적과 크기도 감소된 경향으로 관찰되었다. 이상의 결과로 볼 때 해조류 긴볼레기말 추출물은 고지혈증이 유발된 생쥐 간세포 내에서의 과도한 지방축적을 감소시키는 항고지혈증 효과를 하는 것으로 사료된다. Hepatic tissues of ICR mouse were intraperitoneally injeced with Colpomenia bullosa(CB) Extract after Triton WR-1339(TX) injection were observed to investigate the antihyperlipermic effect of CB extract for hyperlipidemic hepatic tissue caused by destruction of lipid metabolism. The hepatic tissues were obtained at hour-24, 48, and 72 after TX injection with CB extract treatment. And then these specimen were fixed in 10% neutral buffer solution and were cryocut. The tissue stained by H&E for general morphology and sudan black B for lipid distribution. The increase of hepatocyte having rneshlike cytoplasm were shown in all hepatic lobules after TX injection and the hepatic plates were disappeared in the region of meshlike hepatocyte aggregation, But the hepatocyte having meshlike cytoplasm were disappeared and hapatic plate were rearranged in CB extract injected mouse. The number of blue black colored lipid drop in hepatic cytoplasm of mouse injected with TX were increased and the size of lipid drop were enlarged. But the number of lipid drop in hepatic cytoplasm of mouse treated CB extract were decreased and the size of lipid drop were diminished. As results indicated that the accumulation of lipid drop caused by TX injection were mitigated by the antihyperlipidermic effect of CB extract.

連翹敗毒散加味方이 알러지성 접촉피비염에 미치는 影響 : DNCB 재노출부위의 생쥐 피부손상완화와 비만세포 분포 변화를 중심으로 Based on the mitigation of skin damage and distributive change of mast cell on DNCB re-expasure region of Mice

권오성,김진택,박인식,안상현,이해풍,김호현,강윤호 동국대학교 한의학연구소 1999 東國韓醫學硏究所論文集 Vol.8 No.1

본 실험은 連翹敗毒散加味方이 알러지성 접촉피부염 유발시 발생되는 면역과민반응과 염증반응 저해에 미치는 영향을 조사하기위해 행해진 것으로 2,4-dinitrochlorobenzene(DNCB, 5% DNCB 25㎕로 감작, 7일경과 후 2.5% DNCB 5㎕로 유발) 처리로 알러지성 접촉피부염이 유발된 ICR계 암컷 생쥐에 連翹敗毒散加味方抽出物(0.51g/㎏/day)을 음용투여 한 다음 contact hypersensitivity assay, 피부의 일반적인 구조 그리고 비만세포의 분포 변화를 관찰하였다. Contact hypersensitivity assay의 결과, 連翹敗毒散加味方抽出物 투여군의 ear swelling이 DNCB군에 비해 유의성있게 감소한 것으로 나타났다. 알러지성 접촉피부염 유발 후 관찰되는 피부손상은 48시간에 제일 심한 것으로 관찰되었으나, 連翹敗毒散加味方抽出物 투여군에서는 DNCB군 비해 완화된 결과를 보였다. 連翹敗毒散加味方抽出物 투여군의 진피에서 림프구를 비롯한 염증관여세포의 이주와 혈관생성 그리고 표피내로의 림프구의 침윤 등이 감소되었고, 표피 기저층과 가시층세포에서는 세포질내 액포화와 세포사이 공간 확장도 줄어든 것으로 관찰되었다. 한편 알러지성 접촉피부염 유발 후 분포가 증가한 비만세포는 형태가 주로 세포내 함유물이 분비된 degranulated type로 나타났다. 이에 반해 連翹敗毒散加味方抽出物 투여군는 DNCB군에 비해 출현 비만세포'수가 일부 감소했고, 비만세포의 형태는 granulated t-ype였다. 또한 serotonin 면역조직화학적 염색결과로 連翹敗毒散加味方抽出物 투여군에서 se-rotonin 양성반응성이 높은 granulated type의 비만 세포가 주로 관찰되었다. 이상의 결과로 미루어 連翹敗毒散加味方抽出物은 알러지성 접촉피부염으로 유발된 피부 손상과 염증을 완화시키는 효과가 있는 것으로 사료된다. After allergic contact dermatitis elicitated by Dinitrochlorobenzene(DNCB) treatment, ICR female mice administered Yunkyopaedocksangamibang(YPGM) extract were observed to investigate the effect of YPGM on allergic contact dermatitis. This study investigated that contact hypersensitivity assay, abdominal skin morphologic changes including mast cells. At contact hypersensitivity assay, the right ear swelling in YPGM group were probability decrease than DNCB group. At obsevation of abdominal skin morphologic change, the infilteration of lymhocyte, lymphocyte insertion to epithelium, enlarged capillary, angiogenesis, and damages of epithelium as cytoplasmic vacuolation and enlarge of inter cellular space in YPGM were diminshed than DNCB group. The number of mast cell was increased both DNCB and YPGM group. The shape of mast cell in DNCB group was mainly appeared degranulated type, but granulated type in YPGM group. The number of serotonin positive cell was increased both DNCB and YPGM group. The shape of serotonin positive cell in DNCB group was mainly appeared degranuled type, but granulated type in YPGM group. As results indicated that the YPGM exrtact administration work on the mitigation of skin damages in mouse with allergic contact dermatitis.