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호흡기 배상세포에서 폴리양이온성 펩티드에 의해 야기되는 뮤신유리 억제 현상의 특이성 규명
이충재(Choong Jae Lee) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.3
N/A In the present study, we intended to investigate whether polycationic peptides including poly-Llysine (PLL) and poly-L-arginine (PLA) specifically inhibit the mucin release and do not affect significantly the release of the other releasable glycoproteins with less molecular weight than mucin`s from cultured airway goblet cells. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hr and chased for 30 min in the presence of varying concentrations of either poly-L-arginine (PLA) or poly-L-lysine (PLL) to assess the effects on 3H-mucin release and on the total elution profile of the treated culture medium. The results were as follows : (1) PLL 78,000, PLL 9,600 and PLA 8,900 inhibited mucin release in a dose-dependent manner; (2) These polycationic peptides did not inhibit the release of the other releasable glycoproteins with less molecular weights than mucin`s. We conclude that these polycationic peptides `specifically` inhibit mucin release from airway goblet cells. This finding suggests that these polycationic peptides might be used as a specific airway mucin-regulating agent.
Illite 분제 원액의 육모 활성 시험 및 단회 투여 경구 독성시험
이충재(Choong Jae Lee),박형섭(Hyung Sup Park),임동술(Dong Sool Yim),김박광(Bak Kwang Kim),정재훈(Jae Hoon Cheong) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.4
N/A The hair-growth effect of Illite was suggested by some people who were using Illite as a beautifying material. We investigated the hair-growth effect of Illite powder. The hair-growth effects were investigated by two methods; the activity of hair-growth after shaving the hairs on the black mouse (C57BL/6) and the recovery activity of hair-growth after hair-loss induced by cyclophosphamide treatment. Suspension of Illite powder was applied to the back of the black mouse by method of skin paste. Illite promoted significantly the hair growth of mouse in both conditions of shaving and hair-loss. And then we investigated the toxicity which may be induced by Illite when it was administrated orally as a single dose. We could not find out any significant toxicity induced by single dose oral administration of Illite.
생명과학적 연구결과와 성서의 위생학적 기록 및 현대 건강기별의 조화에 관한 일 고찰 (一考察)
이충재 ( Choong Jae Lee ) 글로벌기독교세계관학회 2010 학문과기독교세계관 Vol.1 No.-
In this study, the author tried to investigate whether multitude of biomedical reports can be harmonized with the hygienic records found in the Bible and the Spirit of Prophecy. The three examples including debates on the adequacy of daily water intake recommendation (1.5 liter/day) and intake source of vitamin B12 in absolute vegetarians, based on the published articles in academic journals, were reviewed. On the basis of result of article review, the author suggests that, after all, the natural sciences advocate the concordance of biomedical studies and hygienic records found in the Bible and the Spirit of Prophecy and prove the Bible is God`s revelation, although temporarily the natural sciences fail to support the truths in the Bible.
일차배양 햄스터 기관표면 상피세포에 대한 양이온성 폴리아민의 무독성 효과
이충재(Choong Jae Lee),고광호(Kwang Ho Ko) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.1
In the present study, we intended to investigate whether cationic polyamines including poly-L-lysine (PLL) and poly-L-arginine (PLA) induce cytotoxicities to cultured hamster tracheal surface epithelial (HTSE) cells. Confluent HTSE cells were chased for 30 min in the presence of PLL or PLA of different molecular weights. Possible cytotoxiciries of PLL or PLA were assessed by measuring both Lactate Dehydrogenase (LDH) release during treatment and the number of floating cells after treatment and by checking the possible changes on the morphology of HTSE cells during treatment. The results were as follows: in the case of treatment of PLL or PLA of which molecular weight is about 78,000 and 92,000, respectively, (1) there was significant release of LDH during treatment, (2) the number of floating cells were significantly increased after treatment and (3) there were significant changes on the morphology of cultured HTSE cells. However, in the case of PLL or PLA of which molecular weight is under 10,000 (about 9,600 and 8,900, respectively), no significant signs of cytotoxicities mentioned above were detected. We found that cationic polyamines might be non-toxic under specific range of molecular weights and suggest that the cytotoxicity of cationic polyamine might depend on the molecular sizes of each cationic polyamine.
코로나바이러스 감염증-19(COVID-19)에 대한 의생명과학적 고찰
이충재(Lee, Choong Jae) 삼육대학교 신학연구소 2020 신학과 학문 Vol.29 No.-
본 논문에서는, COVID-19의 병태생리학적 특징과 이 감염증을 치료하기 위한 신약후보물질 개발과 관련된 효과적 전략을 포함한 최근의 정보에 대해 간략히 서술하고자 하였다. SARS-CoV-2에 의한 COVID-19의 세계적 대유행 가능성은, 의약학자들로 하여금 이 질병을 효과적으로 예방 혹은 치료할 수 있는 신약후보물질의 개발에 박차를 가하게 하였다. 현재까지, Umifenovir, Nitazoxanide, Chloroquine, Hydroxychloroquine, Camostat mesylate, Ribavirin, Lopinavir, Ritonavir, Baricitinib, Imatinib, Dasatinib, Cyclosporine, Remdesivir, Favipiravir, Dexamethasone 등의 약물을 대상으로 항바이러스 혹은 적어도 증상조절 효과가 발현되는지 활발히 연구되고 있으나, 아직 인체를 대상으로 한 무작위 이중맹검 임상시험에서 그 유효성과 안전성이 입증된 약물은 없는 실정이다. 결론적으로, COVID-19의 종식 혹은 극복을 위해 이 질병의 병태생리학적 특성을 더 상세히 파악하고 감염 증후군을 효과적으로 통제 가능한 치료용 신약을 개발할 수 있는 혁신적 전략이 절실히 요구되고 있다. In the present study, the author tried to review the pathophysiology of coronavirus disease 2019(COVID-19) and the strategy for the development of novel therapeutic agent to regulate COVID-19. The pandemic status of COVID-19 provoked by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) urges the medical scientists to search the potential efficacious drugs for the prevention and treatment of COVID-19. To date, Umifenovir, Nitazoxanide, Chloroquine, Hydroxychloroquine, Camostat mesylate, Ribavirin, Lopinavir, Ritonavir, Baricitinib, Imatinib, Dasatinib, Cyclosporine, Remdesivir, Favipiravir, Dexamethasone have been examined whether each agent manifests the potential antiviral and/or disease-modifying activities. However, no agent has been proven to be efficacious in randomized double-blind clinical trials. Therefore, it is essential to develop the optimal novel drug candidate through future study, based on the expedited understanding of pathobiology and clinical features of COVID-19.
폴리 - 엘 - 아르기닌이 햄스터 기도 배상세포에서의 뮤신 유리 및 흰쥐 기도 배상세포내 함유된 점액에 미치는 영향
이충재(Choong Jae Lee) 한국응용약물학회 2001 Biomolecules & Therapeutics(구 응용약물학회지) Vol.9 No.4
N/A In this study, we tried to investigate whether poly-L-arginine (PLA) (MW 10,800) significantly affect mucin release from cultured hamster airway goblet cells and the mucosubstances of hypersecretory airway goblet cells of rats. Confluent primary hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with ^3H-glucosamine for 24 hr and chased for 30 min in the presence of varying concentrations of PLA to assess the effects on ^3H-mucin release. Possible cytotoxicities of PLA were assessed by measuring both Lactate Dehydrogenase (LDH) release and by checking the possible changes on the morphology of HTSE cells during treatment. For in vivo experiment, hyperplasia of rat airway goblet cells and increase in intraepithelial mucosubstances were induced by exposing rats to SO_2 for 3 weeks and varying concentrations of PLA were administered inhalationally to assess the effects on the mucosubstances of airway goblet cells of rats. The results were as follows : (1) PLA significantly inhibited mucin release from cultured HTSE cells in a dosedependent manner; (2) there was no significant release of LDH and no significant change on the morphology of cultured HTSE cells during treatment; (3) PLA also affected the intraepithelial mucosubstances of hypersecretory rats and restored them to the levels of control animals. We conclude that PLA inhibit mucin release from airway goblet cells without significant cytotoxicity and possibly normalize the hypersecretion of airway mucosubstances in vivo. This finding suggests that PLA might function as an airway mucoregulative agent.
인간 기도 상피세포에서 증가된 뮤신 생성에 대한 쿠마린과 인도메타신의 억제작용
이재우(Jae Woo Lee),김길동(Kil-Dong Kim),전병규(Byeong Kyou Jeon),이충재(Choong Jae Lee) 대한약학회 2010 약학회지 Vol.54 No.5
We examined whether indomethacin, noscapine, coumarin, uridine and betaine affect airway mucin production induced by EGF or TNF-α from NCI-H292 cells. Cells were pretreated with each agent for 30 min and then stimulated with EGF or TNF-α for 24 h. Of the five compounds, coumarin suppressed airway mucin production induced by EGF or TNF-α. However, indomethacin suppressed airway mucin production induced by EGF. This result suggests that coumarin and indomethacin can regulate the production of mucin induced by EGF, by directly acting on airway epithelial cells.