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돼지 난소내 과립세포의 염색질이상응축과 여포액내 스테로이드호르몬의 농도 변화와의 상관관계
이창주,윤용달,김종흡,김문규,Lee, Chang-Joo,Yoon, Yong-Dal,Kim, Jong-Heup,Kim, Moon-Kyoo 대한생식의학회 1989 Clinical and Experimental Reproductive Medicine Vol.16 No.1
In order to study the follicular atretic mechanism in mammalian ovary, the relationship between pyknotic index (PI) of granulosa cells and the steroid concentrations in the follicular fluid of atretic follicle was investigated. Follicles were isolated from porcine ovary according to their sizes and the reproductive phases. Steroid concentrations were quantified by high-performance liquid chromatography. As PI increased, the concentration of progesterone was significantly increased(p<0.05), whereas testosterone and estradiol showed no significant changes in their concentration. As follicular size was increased, PI of follicular GC in the luteal phase was increased significantly(p<0.05) and the molar ratio of progesterone to testosterone was increased in the follicles of follicular phase. It can be concluded that progesterone accumulated in the follicular fluid as atresia of the follicles was progressed, and that PI of granulosa cells could be used as one of convenient and pratical criteria for the identification of follicular atresia.
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공황발작으로 응급실에 내원한 공황장애 환자들의 임상 특징
이창주,남범우,손인기,Lee, Chang-Ju,Nam, Beom-Woo,Sohn, In-Ki 한국정신신체의학회 2021 정신신체의학 Vol.29 No.1
Objectives : This study was designed to investigate datas related to panic attack and treatment in emergency room of panic disorder patients who visited emergency room for panic attack. Methods : A retrospective analysis of medical records was conducted on 92 patients with panic disorder who visited Chungju Konkuk university hospital emergency department due to panic attack and had bodily symptoms from 1st January 2010 to 31th December 2019. In addition to demographic characteristics and comorbid disorders, triggering stressors and alcohol consumption were corrected as pre-panic attack datas, bodily symptoms at the time of panic attack were corrected as datas during attack, electrocardiogram trial, consultation with psychiatrist, admission and information of used psychotropic drugs were corrected as post-attack data. Depending on size of data, Chi-square test or Fisher's exact test was used. Collected data was analyzed using R 4.03. Results : Cardiovascular disease was accompanied by 5.4% and depressive disorder was the most common coexisting mental disorder. Among triggering stressors, economic problem/work-related stress was significantly higher in men than women (𝛘<sup>2</sup>=4.322, p<0.005). The most common physical symptom during attack was circulatory (65.2%), followed by respiratory (57.6%), numbness-paralysis (33.7%), dizziness (19.6%), gastro-intestinal (14.1%) and autonomic symptom (12.0%). Electrocardiogram was taken at higher rate when patients complained circulatory symptom (𝛘<sup>2</sup>=8.46, p<0.005). The psychotropic drug most commonly used in emergency room was lorazepam, used in 92.1%. Conclusions : The most common bodily symptom during panic attack was circulatory symptom and the most common triggering stressor in men was economic problem/work-related stress. The most commonly used psychotropic for panic attack was lorazepam.
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무지개송어에서 분리된 IHNV (감염성 조혈 괴사바이러스) 유전자형에 따른 병원성 비교
이창주,김광일,한유선,제갈명은,김영진,Lee, Chang-Ju,Kim, Kwang-Il,Han, Yu-Seon,Jegal, Myeong-Eun,Kim, Yung-Jin 한국생명과학회 2021 생명과학회지 Vol.31 No.6
This study investigated the pathogenicity of different genotypes of infectious hematopoietic necrosis virus (IHNV) strains isolated from infected rainbow trout in Gangwon Province. RtPc0314c and RtPc0314g strains belonging to the JRt-Shizuoka lineage and RtPc0816g strain belonging to the JRt-Nagano lineage showed 100% cumulative mortality when inoculated at a high titer. In addition, more rapid necrosis was observed in rainbow trout infected with RtPc0314c and RtPc0314g mutations. When inoculated at a low titer, 100% mortality was not observed until the end of the experiment, but the mortality was higher in rainbow trout infected with a mutant strain belonging to the JRt-Shizuoka linage than a mutant strain belonging to the JRt-Nagano lineage. A histopathological analysis showed a clear signature of infection in kidney and spleen tissues upon infection with RtPc0314c and RtPc0314g but no signature of infection associated with the Rt03186 strain. Based on the results in this study, it seems that strains belonging to the JRt-Shizuoka lineage in Gangwon Province IHNV are more pathogenic.
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Follicular Lactate Dehydrogenase Activity and Steroid Concentrations in the Immature Gilt Ovary
이창주,윤용달,Lee, Chang Joo,Yoon, Yong-Dal The Korean Society for Reproductive Medicine 2005 Clinical and Experimental Reproductive Medicine Vol.32 No.3
연구목적: 난포가 폐쇄되는 동안의 생화학적 변화를 규명하기 위하여 미성숙 돼지의 정상 및 폐쇄 난포 내 lactate dehydrogenase (LDH) 활성도 변화 및 동일 난포액 내 스테로이드호르몬의 농도변화를 알아보기 위하여 본 연구를 시행하였다. 재료 및 방법: 난포액 (FF), 과립세포 (GC), 협막세포 (TC) 내 LDH의 활성도를 측정하였으며, 난포액 내 progesterone ($P_4$), testosterone (T), estradiol ($E_2$)의 농도변화를 방사면역측정법으로 정량하였다. 결 과: 정상 및 폐쇄 난포에서 $P_4$의 농도변화를 보이지 않았다. 그러나 폐쇄 난포액 내 T의 농도($3.85{\pm}1.50ng/ml$)는 정상 난포 ($1.29{\pm}0.54ng/ml$)에 비해 현저히 높았으며 정상 난포 내 $E_2$의 농도 ($43.29{\pm}19.51ng/ml$) 는 폐쇄 난포 ($18.82{\pm}7.27ng/ml$)에 비해 현저히 높은 것으로 나타났다. 정상 난포액 내 $P_4$의 농도는 난포의 크기에 정의 상관관계 (r=0.75)를 보였다. 정상 난포 내 T:$P_4$의 비율 ($8.14{\pm}3.35$)은 폐쇄 난포 ($1.39{\pm}0.60$)에 비해 현저히 높았으며, 정상 TC ($433.63{\pm}102.40{\mu}U/{\mu}g$ DNA) 및 FF ($246.86{\pm}58.96{\mu}U/{\mu}l$) 내 LDH 활성도는 폐쇄 난포 (각각 $83.7{\pm}10.5$와 $38.71{\pm}9.00$)에 비해 현저히 높게 나타났다. 정상 난포의 GC 및 FF 내 LDH 활성도는 $E_2$의 농도와 부의 상관관계를 보였지만, 폐쇄 난포의 TC 내 LDH 활성도는 $P_4$, T, $E_2$의 농도에 대해 정의 상관관계를 나타내었다. 결 론: 본 실험의 결과, 미성숙 돼지 난포의 폐쇄는 TC 내 LDH 활성도 감소와 밀접한 관계를 갖는 것으로 사료된다.
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상완골 과간 분쇄골절에서 L-형의 관절외 주두절골술 후 Y-형 금속판 고정술
이창주(Chang Ju Lee),조원호(Won Ho Cho),장호근(Ho Guen Chang),이응주(Ung Joo Lee),장준동(Jun Dong Chang),이상수(Sang Su Lee) 대한골절학회 1996 대한골절학회지 Vol.9 No.4
Intercondylar fractures of the distal humerus in adults are rare and notoriously difficult to treat. We treated 8 patients by open reduction and internal fixation using Y-plate with extraarticular olecranon osteotomy in posterior approach. L-shaped osteotomy was performed at the extraarticular portion of olecranon with thiceps tendon insertion remained to proximal portion of it, using air-saw. The follow- up period ranged from l2 to 30 months with average of 19.7 months. The fractures were classified according to AO classification. The results were evaluated using Jupiter et al grading system. 1) There was no loosening of fixation device of humeral condyle and olecranon., 2) Niether delayed union nor nonunion of olecranon and humeral condyle were found. 3) Exellent grade was achieved in 4 patients(50%), three(38%) had good. and one(12%) poor. Flexion-extension arc ranged from 60 degree to 120 degree with mean of 98.8 degree. 4) Complications included postoperative neuritis in one, myositis ossificance in one, and heterotopic bone in one patient. Authors would introduce the method and result of extraarticular olccranon osteotomy in posterior approach for the intercondylar fracture of distal humerus, as a new technique.
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