안동호 상류 운곡천의 이화학적 수질특성과 식물플랑크톤 군집 특성

이중복,이희무,이건주,박정원,박재충,김동걸,권기석 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1

This investigation is about the characteristics of phytoplankton community and physciochemical water quality of specific 6-point the Woon-kog stream system in upsteam of the Andong Lake. DO value was showed over 8.1㎎/L at each site and COD_Mn, BOD, T-N, T-P tend to increase as they stream down and that the existence and dominance of phytoplankton was low and it was difficult to conclude the definite correlation of water quality and phytoplankton community. Finally, it seemed to be desirable that alternatives for pollutional reduction should be made and performed on the basis of the continuous monitoring of the inflow to preserve the Andong Lake.

Biodegradation of Organochlorine Insecticide Endosulfan by the Fungus Eutypella sp. KEF-1

이중복,박상열,신기선,전춘표,김장억,권기석 한국환경농학회 2011 한국환경농학회지 Vol.30 No.2

BACKGROUD: α- and β- Endosulfan isomers of endosulfan, an endocrine disrupting chemical, are widely used cyclodiene organochlorine pesticide in worldwide, and it has widespread application in agriculture and can contaminate river-system as runoff from soil or aerial deposition METHOD AND RESULTS: In this study, an attempt was made to isolate an endosulfan degrading fungus from endosulfan-polluted agricultural soil. Through repetitive enrichment and successive subculture in media containing endosulfan and its metabolites as the sole carbon source, a fungus designated KEF-1 was isolated. Based on phylogenetic analysis, strain KEF-1 was assigned to the genus Eutypella. Also, the ITS (internal transcribed spacer) sequences of KEF-1 were submitted to GenBank under accession number EF581006. In potato dextrose broth containing 8 µg/mL endosulfan, strain KEF-1 completely degraded the endosulfanin 12 days. CONCLUSION: These results suggested that Eutypella sp. KEF-1 has potential as a biocatalyst for endosulfan bioremediation

돼지 오제스키병(病)에 관한 연구(硏究): 1. 감염자돈(感染仔豚)으로 부터 원인체의 분리(分離) 및 동정(同定)

이중복,안수환,김병한,송재영,김용희,설동섭,Lee, Jung-bok,An, Soo-hwan,Kim, Byoung-han,Song, Jae-young,Kim, Yong-hee,Sul, Dong-sup 대한수의학회 1988 大韓獸醫學會誌 Vol.28 No.1

The first outbreak of Aujeszky's disease(AD) was identified from piggery located at the southern part of Korea in July, 1987. This piggery suffered from a significant economic loss caused by unexpected piglet mortality and reproductive failure. Etiologic viral agents were isolated from tonsil and spleen of the infected piglets, and the isolates produced a typical cytopathic effects of herpesvirus with giant cell formation when inoculated in many different cells. Subsequently the field isolates were characterized as suid herpesvirus I by cross-neutralization test and indirect fluorescence assay utilizing specific monoclonal antibody, and were proved to be a pathogenic strain of AD virus(ADV).

Induction of castration by immunization of male dogs with recombinant gonadotropin-releasing hormone(GnRH)-canine distemper virus(CDV)T helper cell epitope p35

이중복,정미정,문영찬,조익현,예정옹,김선근,장화석,박성영,송창선,김휘율,박근규,Steven McOrist,최인수 대한수의학회 2005 Journal of Veterinary Science Vol.6 No.1

Immunocastration is a considerable alternative to a surgical castration method especially in male animal species for alleviating unwanted male behaviors and characteristics. Induction of high titer of antibody specific for gonadotropin-releasing hormone (GnRH) correlates with the regression of testes. Fusion proteins composed of canine GnRH and T helper (Th) cell epitope p35 originated from canine distemper virus (CDV) F protein and goat rotavirus VP6 protein were produced in E. coli. When these fusion proteins were injected to male dogs which were previously immunized with CDV vaccine, the fusion protein of GnRH-CDV Th cell epitope p35 induced much higher antibody than that of GnRH-rotavirus VP6 protein or GnRH alone. The degeneration of spermatogenesis was also verified in the male dogs immunized with the fusion protein of GnRH-CDV Th cell epitope p35. These results indicate that canine GnRH conjugated to CDV Th cell epitope p35 acted as a strong immunogen and the antibody to GnRH specifically neutralized GnRH in the testes. This study also implies a potential application of GnRH-based vaccines for immunocastration of male pets.

식품 및 환경, 기타 Biphenyl의 Sphingobium yanoikuyae BK-10에 의한 분해 특성

이중복 ( Jung Bok Lee ),김동걸 ( Dong Geol Kim ),최충식 ( Chung Sig Choi ),손호용 ( Ho Yong Sohn ),김장억 ( Jang Eok Kim ),권기석 ( Gi Seok Kwon ) 한국미생물생명공학회 2006 한국미생물·생명공학회지 Vol.34 No.2

고추역병 유발병원균 Phytophthora capsici에 대한 Bacillus sp. AM-651의 항진균활성

이중복 ( Jung Bok Lee ),신정학 ( Jeong Hak Shin ),장종옥 ( Jong Ok Jang ),신기선 ( Kee Sun Shin ),최충식 ( Chung Sik Choi ),김건우 ( Kun Woo Kim ),조민섭 ( Min Sub Jo ),전춘표 ( Chun Pyo Jeon ),김윤회 ( Yun Hoi Kim ),권기석 ( Gi S 한국미생물 · 생명공학회 2008 한국미생물·생명공학회지 Vol.36 No.3

부추 당침액의 유산균 발효에 따른 항산화 및 ACE저해활성

이중복(Jung-Bok Lee),배정식(Jung-Shik Bae),손일권(Il-Kwon Son),전춘표(Chun-Pyou Jeon),이은호(Eun-Ho Lee),주우홍(Woo-Hong Joo),권기석(Gi-Seok Kwon) 한국생명과학회 2014 생명과학회지 Vol.24 No.6

본 연구는 최근 국민들이 많은 관심을 갖는 식물발효 효소액 중 부추 당침액(부추:설탕 1:3)을 유산균(Lactobacillus acidophilus AML 0422, Lactobacillus brevis HLJ 59, Lactobacillus helveticus AML0410, Lactobacillus plantarum KCTC 13093) 발효를 통해 발효 부추액의 항균활성 및 항산화활성과 항고혈압활성 등의 기능성을 발효 전 후의 유효 특성을 조사하였다. 유산균을 이용하여 발효한 발효 부추액은 S. aureus KCTC 1916에 대해 31.43 mm의 높은 항균력을 보이는 것으로 조사되었으며, 총 폴리페놀함량, 총 플라보노이드 함량과 DPPH 활성 소거능이 160.8-178 mg/ml, 100-108 mg/ml, 82.8-93.5%로 각각 조사되었다. DPPH 활성 소거능에 있어서는 Vitamin C 50 ppm과 유사한 활성을 보이는 것으로 나타났다. 심혈관질환 및 뇌혈관질환 등 고혈압과 관련이 깊은 ACE 저해활성은 유산균의 종류에 따라 50.4-67%로 발효전인 32.6%보다 좋은 활성을 보이는 것으로 조사되었다. 이상의 결과를 종합하였을 때 유용 유산균을 이용한 부추 당침액의 발효산물은 항균활성, 항산화활성과 ACE 저해활성 등 우수한 생리활성 효과를 나타내는 것으로 조사되었다. In recent years, the growing interest in the health care benefits of sugared-plant fermented enzymes has led to increased consumption. This study investigated the fermentation of sugared-buchu (Leek:sugar, 1:3) by lactic acid bacteria (Lactobacillus acidophilus AML 0422, Lactobacillus brevis HLJ 59, Lactobacillus helveticus AML0410, Lactobacillus plantarium KCTC 13093) and the antibacterial activity, antioxidant activity, and functionality (e.g., anti-hypertensive activity) of the fermented product. The fermented sugared-buchu showed high antibacterial activity against Staphylococcus aureus KCTC 1916, at 31.43 mm, and its total polyphenols, total flavonoid content, and DPPH scavenging activity were 160.8-178 mg/ml, 100-108 mg/ml, and 51.4-58.1%, respectively. DPPH scavenging activity was to that of vitamin C (50 ppm). ACE inhibitory activity was 50.4-67%, depending on the strain of lactic acid bacteria, and the control of sugared-buchu activity was higher than 32.6%. These results suggest that sugared-buchu fermented with lactic acid bacteria has strong antibacterial, antioxidant, and ACE inhibitory activities.

스마트IT기기를 활용한 농가일원화시스템 설계 및 적용 -농가 일원화 시스템 설계-

이중복 ( Jung-bok Lee ),윤재상 ( Jae-sang Yun ) 한국국제농업개발학회 2015 韓國國際農業開發學會誌 Vol.27 No.5

본 연구에서는 안동지역 특용작물 재배농가의 요구를 반영하여 사용자 편이성이 강화된 정보화의 방안으로 스마트폰을 활용한 정보화시스템 구축을 다음과 같이 검토하였다. 본 연구의 방법은 연구의 대상인 안동지방 특용작물재배농가에 대한 정보화요구 자료수집과 분석, 실제 사용자인 특용작물 생산농가의 의견수렴을 거쳐 DB의 구축, 프로그램레이 아웃을 거쳐 농업경영장부와 영농일지가 반영된 일원화시스템구축을 하였으며, 산출물은 농민들의 시연을 거쳐 보완하였다. 연구의 결과는 다음과 같다. 1. 스마트폰을 기반으로 한 판매관리시스템은 정보화에 익숙하지 않은 농민들도 쉽게 사용할 수 있어 농가경영개선에 도움을 줄 수 있었다. 2. 스마트폰연계 영농시스템은 컴퓨터와 연동하여 웹기반 영농일지 및 영농관리에 활용할 수 있었다. 3. 소비자 입장에서는 친환경농산물 생산에 필요한 생산이력관리를 제공할 수 있었다. 향후 농업인구 감소와 급속한 고령화의 진행은 농업산업의 변화를 요구하고 있으며 본 연구가 스마트시대로의 변화 속에 농촌정보화의 한 축을 담당할 수 있을 것이다. This paper designed a highly reliable high-precision agriculture management integrated system using smart IT devices(Android) and PC. Smart farming daily and the mobile farming daily have been developed in recent years but still the majority of managers may be written by hand. However, the hand-written farming daily, there is no way that you can manage the data directly. In addition, the problem is that there is no way to determine if users are not wrong to write. It is possible to write an agricultural management ledger and a lot number farming daily a centralized monitoring system of agricultural business management database easily by taking advantage of this system. The system of this paper is a real-time farming business model that can be used for production and sales forecasting data. This system is a centralized system to support for the statistics, customer, product, order, and settlement that not constrained by time and place.

국내 공수병의 발생현황 및 예방대책

이중복(Joong-Bok Lee),이훈재(Hun-Jae Lee),현방훈(Bang-Hun Hyun),방지환(Ji-Hwan Bang),남경옥(Kyung-Ok Nam),정영의(Young-Eui Jeong),신영학(Young-Hack Shin) 한국역학회 2005 Epidemiology and Health Vol.27 No.1

Rabies is a uniformly fatal encephalitis and cannot be treated, therefore efforts must be focused on preventing the disease. The incidence of rabies in humans and animals has decreased with the introduction of rabies vaccination for animals since the early 1950s in Korea. There was no rabies occurrence either in human or animal for 8 years from 1985 to 1992. However, a case of animal rabies recurred in 1993. Since then, rabies endemic areas were expanded into 17 counties near the demilitarized zone, and 6 human cases were reported from the endemic areas of animal rabies. According to epidemic studies, wild raccoon dogs were suspected to be the transmission source of rabies in dogs, domestic animals, and other wild animals, which resulted in human rabies. Rabies prevention strategies were aimed at the animals capable of transmitting rabies or vaccination for the high risk group and postexposure prophylaxis for the bitten patients. Unfortunately, these activities were not conducted appropriately and substantially. All rabies victims were either not treated or did not receive timely and appropriate postexposure treatment. Prevention of rabies can only be achieved by securing political and financial support for an effective rabies program. Key activities for rabies prevention should include changing current public perception regarding rabies, strengthening surveillance for the exposed person to suspected rabid animals, and laboratory-based rabies surveillance.

Lactic acid bacteria를 이용한 마늘 고체 발효에 따른 생리활성

이중복(Jung-Bok Lee),주우홍(Woo-Hong Joo),권기석(Gi-Seok Kwon) 한국생명과학회 2016 생명과학회지 Vol.26 No.4

마늘(Allium sativum L.)은 많은 아시아 지역의 나라에서 민간요법 및 추출물을 이용하여 다양하게 사용하고 있다. 국내 전통채소류들은 다양한 기능성과 강한 생리학적 활성을 가지고 있으며, 기능성 식품재료로 사용이 가능하다. 본 연구는 유산균을 이용하여 고체마늘 발효에 따른 생리활성을 조사하고자 하였다. 마늘 고체 발효를 위해 유산균을 한국전통 발효식품인 젓갈로부터 분리 및 KCTC로 부터 분양 받아 사용하였다. 발효유산균 선별은 MRS 고체배지에 디스크법을 이용하여 마늘 착즙액에 저항을 보이며 증식하는 유산균을 선택하였다. 선별된 유산균을 이용한 마늘 고체발효 추출물의 총 폴리페놀과 플라보노이드 농도가 731.0-845.2 ug/g과 92.68-413.58 ug/g로 각각 조사되었다. 그리고 DPPH 라디컬 활성과 SOD 유사활성은 양성대조구인 비타민 C 50 ppm 보다 높은 활성을 보였다. 또한, 항당뇨 활성인 α-glucosidase 저해활성은 유산균 발효마늘에서 양성대조구인 acarbose 50 ppm 보다 높은 저해활성을 보이는 것으로 조사되었다. 본 연구결과 유산균 발효 마늘의 생리학적 활성을 증대를 통해 향후 항산화 활성과 항당뇨 활성을 갖는 유산균 발효 마늘의 개발을 위해 유산균을 활용이 가능하며, 발효물을 이용하여 기능성 식․음료 소재로의 개발 가능성을 보일 것으로 사료된다. Garlic (Allium sativum L.) and its extracts have been used in a wide range of applications, including as folk medicines in many Asian countries. This traditional herb has several functional properties and strong biological activities, making it useful as a functional food material. This study investigated the biological activities of solid-garlic after fermentation by lactic acid bacteria. Several solid-garlic fermentation strains of lactic acid bacteria were isolated from Korean traditional fermented food or obtained from the Korean Collection for Type Cultures. Lactic acid bacteria showed selective growth in garlic extracts on MRS media. Fermentation of solid garlic (heated 121℃, 15 min or not heated) by lactic acid bacteria gave total polyphenol and flavonoid contents of 731.0-845.2 g/g and 92.68-413.58 g/g, respectively. The DPPH scavenging activities and SOD like activities were measured as 7,584% and 9499%, respectively. These activities were relatively higher than a positive control, vitamin C. Measurement of antidiabetic activity using α-glucosidase inhibition assay showed that solid garlic fermented with lactic acid bacteria gave a higher activity than the control, acarbose. Fermentation of solid garlic with lactic acid bacteria may therefore help to alleviate adverse biological activities, as well as provide functional food materials.