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- 저자 : 이중복(Jung?Bok Lee) (검색결과 5 건)
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안동호 상류 운곡천의 이화학적 수질특성과 식물플랑크톤 군집 특성
이중복,이희무,이건주,박정원,박재충,김동걸,권기석 7개 국립대학교 환경연구 논문집 공동발행 위원회 2002 환경연구논문집 Vol.2 No.1
This investigation is about the characteristics of phytoplankton community and physciochemical water quality of specific 6-point the Woon-kog stream system in upsteam of the Andong Lake. DO value was showed over 8.1㎎/L at each site and COD_Mn, BOD, T-N, T-P tend to increase as they stream down and that the existence and dominance of phytoplankton was low and it was difficult to conclude the definite correlation of water quality and phytoplankton community. Finally, it seemed to be desirable that alternatives for pollutional reduction should be made and performed on the basis of the continuous monitoring of the inflow to preserve the Andong Lake.
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Baculovirus를 이용한 Aujeszky's Disease Virus gⅢ 단백질 발현
송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-
The g Ⅲ gene located in U_L region of Yangsan strain, a field isolate of Aujeszky's disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The gⅢ gene consisting of 1,437 nucleotides showed 98% sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gⅢ of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed gⅢ was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-gⅢ monoclonal antibody. The partially purified gⅢ protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect to specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gⅢ protein antigen (98%) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false nagative reactions in gⅢ RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.
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국내에서 분리된 Infectious Bovine Rhinotracheitis Virus DNA의 제한효소분석
민원기,전무형,이중복,김병한,안수환 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
Nine strains of infectious bovine rhinotracheitis virus(IBRV) isolated in Korea since 1970 were investigated to elucidate the pathogenicity on various cell lines and the reactivity in cross serum neutralization test. The genomes of the viruses were also studied by restriction endonuclease analysis to examine the genetical patterns and the origin of the viruses. Results obtained by experiment were as follows ; 1. In pathogenicity test on various cell lines by cytopathology and immunofluorescence antibody assay, MDBK cells were found to be very susceptible to all of the domestic isolates, Colorado and Oxford strains and RK-13 cells, mildly reactive to all of viruses. However, CV-1 and BHK cells revealed negligible reactions against all of viruses tested. 2. In cross serum neutralization(SN) test with the positive serum from the cattle immunized with PQ7 strain of IBRV, PQ7, SQ, VS, A14, TSV and S′74-7 strains showed 64 of SN titer, and IQ, QW, A37, Colorado and Oxford strains, 128 of SN titer. It was assumed that there is no difference in major antigenic determinants among the viruses. 3. By analysis of restriction endonuclease Hind Ⅲ, it was found that 8 domestic isolates except A37 and Colorado strain revealed the identical banding patterns. However, Oxford strain showed quite different patterns, displaying H band at 6.9×10 exp (6) daltons and K′band at 4.5×10 exp (6) daltons. A37 strain exclusively contained the longer bands of A′ and A″. 4. When the viral DNA were analyzed by restriction endonuclease EcoRI, all of the domestic isolates and Colorado strain showed very similar banding patterns, whereas Oxford strain revealed a considerable discrepancy, displaying B′ band at 12.5×10 exp (6) daltons. 5. By analysis of restriction endonuclease BamHI, it was found that the domestic isolates and Colorado strain revealed similar patterns displaying 8 fragments. However, Oxford strain was cleaved into 9 fragments displaying G′and G″ bands.
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국내에서 분리된 canine parvovirus의 구조유전자 cloning과 염기서열 분석
박종현,송재영,이중복,현방훈,안수환,전무형 충남대학교 생물공학연구소 1993 생물공학연구지 Vol.3 No.-
In this study gene encoding structural proteins of a CPV isolate was cloned and saquenced to elucidate the molecular genetical properties of the canine parvoviruses isolated from the field. Six recombinant plasmids of pEP3, p1471, p2070, pEP069, pEP338 and p14711p were constructed from the map positions 22 to 98 of RF DNA to clone the VP1 and VP2 genes of CPV-V20. Sequentialy the gene comprising 3780 nucleotides were sequenced by dideoxy chain termination method. When nucleotide sequence of gene encoding the structural proteins of CPV-V20 was compared with those of other strains, CPV-N, CPV-d and CPV-780929 published previously. DNA homologies to CPV-V20 were 99.87% with CPV-NM, 99.73% with CPV-d, 96.85% with CPV-780929 AND 98.4% with FPLV-Carl, respectively. The DNA sequence data of CPV-V20 showed seven point mutations and also deletion of 135 nucleotides from the nucleotide position 4745 to 4879 located in the 3-noncoding region of CPV-N.
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全茂炯,金德煥,安壽煥,李重馥,閔元其 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-
To develop more specific and sensitive diagnostic methods for infectious bovine rhinotracheitis, 7-C-2 monoclonal antibody specific to polypeptides of infectious bovine rhinotracheitis virus (IBRV) was applied in indirect immunofluorescence antibody assay(IFA), indirect immunoperoxidase assay(IPA) and radial immunodiffusion enzyme assay (RIDEA). It was found that IBRV infected in MDBK cells could be detected as early as 8 hours post infection by IFA, and that IFA was more rapid and specific to identify IBRV antigen than IPA. The diagnostic efficacy of RIDEA and SN test was studied with 88 bovine sera. It was evident that RIDEA could eliminate the false positive reaction encountered in serum neutralization(SN) test, being more rapid and sensitive than the latter. Highly significant correlation coefficiency(r=0.76, p<0.01) was evaluated between the titers of sera and the diameters of RIDEA. Tracheal membranes and sera collected from 96 slaughtered cattle with lesions in respiratory organs were examined to detect IBRV antigen and antibody by IFA, RIDEA and SN test. It was presented that positive rates were 32.3% in IFA, 20.8% in RIDEA and 21.9% in SN test, and that coincidence rate between RIDEA and SN test were 100% in positive sera and 98.7% in negative sera. In conclusion, it was assumed that application of monoclonal antibody could improve the diagnostic efficacy of IBR by enhancing sensitivity and specificity of IPA, IFA and RIDEA.
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