원료의약품 분진의 폭발 위험성 평가

이주엽,이근원,박상용,한인수,Lee, Joo Yeob,Lee, Keun Won,Park, Sang Yong,Han, In Soo 한국화학공학회 2017 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.55 No.5

Hazard risk of explosion on pharmaceutical raw materials dust in pharmaceutical industry often exists when it is handled or processed in the industrial sites, and explosion accident is caused by this. In this study, the dust explosion characteristics of the three pharmaceutical raw materials samples were measured. The main explosion characteristics are as follows: $P_{max}$, MIE and MIT of loxoprofen acid having $5.31^{\circ}C$ of median diameter are obtained 8.4 bar, 1 mJ < MIE < 3 mJ and $550^{\circ}C$. $P_{max}$, MIE and MIT of camphorsulfonate having $95.63^{\circ}C$ of median diameter are obtained 7.9 bar, 30 mJ < MIE < 100 mJ and $510^{\circ}C$. $P_{max}$, MIE and MIT of rifampicine having $26.48^{\circ}C$ of median diameter are obtained 7.9 bar and 1 mJ < MIE < 3 mJ and $470^{\circ}C$. The deflagration index ($K_{st}$) and the explosion index (EI) were obtained by using these data. The explosion hazard assessment of pharmaceutical raw materials dust was compared and examined. As a result, the explosion hazard assessment according to deflagration index and explosion index were the explosion class with St 2 and the explosion hazard rating of severe for loxoprofen acid & rifampicine and St 1 and strong for clopidogrel camphorsulfonate, respectively. 산업현장에서 취급되거나 가공되는 원료의약품 분진의 폭발 위험성은 항상 존재하며, 이로 인한 폭발사고가 자주 발생되고 있다. 본 연구에서는 원료의약품 시료 3종의 분진폭발특성을 측정하였다. 주요 폭발특성 측정값은 록소프로펜산은 평균 입경이 $5.31{\mu}m$이며, $P_{max}$는 8.4 bar, 최소점화에너지는 1 mJ < MIE < 3 mJ이며 최소점화온도는 $550^{\circ}C$이다. 클로피도그렐 캄포르술폰산염은 평균 입경이 $95.63{\mu}m$이며, $P_{max}$는 7.9 bar, 최소점화에너지는 30 mJ < MIE < 100 mJ이며 최소점화온도는 $510^{\circ}C$이었다. 리팜피신은 평균 입경이 $26.48{\mu}m$이며 $P_{max}$는 7.9 bar, 최소점화에너지는 1 mJ < MIE < 3 mJ이며 최소점화온도는 $470^{\circ}C$로 나타났다. 이들 값을 적용하여 폭연지수($K_{st}$)와 폭발지수(EI)의 폭발위험등급을 구하고, 원료의약품 분진의 폭발 위험성을 비교 검토하였다. 그 결과 폭발 위험성은 록소프로펜산과 리팜피신의 폭발등급은 St 2이고 폭발위험등급은 severe이며, 클로피도그렐 캄포르술폰산염의 폭발등급은 St 1이고 폭발위험등급은 strong으로 나타났다.

형개(荊芥)가 조골세포(造骨細胞)에 미치는 영향(影響)

이주엽,황귀서,Lee, Joo-Yup,Hwang, Gwi-Seo 대한예방한의학회 2009 대한예방한의학회지 Vol.13 No.3

Objectives : The author aimed to evaluate the effect of BuOH fraction(ST) from Schizonepeta tenuifolia on osteoblast proliferation in murine calvarial cells. Methods : The osteoblast separated from murine calvariae was cultivated for 10 days and evaluated the cell function. After the addition of ST on the culture medium, we determined the effect of ST on the cell proliferation, protein synthesis, alkaline phosphatase activity, collagen synthesis, and apoptosis of the osteoblast. Results : 1. ST increased the proliferation of osteoblast, and restored the decreased cell number in glucocorticoid (GC)-treated osteoblast. 2. ST increased protein synthesis of osteoblast, and restored the decreased protein synthesis in GC-treated osteoblast. 3. ST increased ALP activity of osteoblast, and restored the decreased enzyme activity in GC-treated osteoblast. 4. ST increased collagen synthesis of osteoblast, and restored the decreased collagen synthesis in GC-treated osteoblast. 5. ST did not change the survival rate of osteoblast, but increased the survival rate in GC-treated osteoblast. Conclusions : It is concluded that ST might reduce the osteoporosis resulted from augumentation of osteoblast proliferation.

월상 골 제거 및 혈관부착 두상 골 이식으로 치료한 진행된 키엔벡 병

이주엽,김형민,정양국,진성기,박일중,Lee, Joo-Yup,Kim, Hyoung-Min,Chung, Yang-Guk,Jin, Sung-Ki,Park, Il-Jung 대한미세수술학회 2008 Archives of reconstructive microsurgery Vol.17 No.2

Purpose: There are many controversies concerning therapeutic guidelines for the treatment of Kienb$\ddot{o}$ck's disease. The purpose of this study is to evaluate the clinical and radiological result of lunate replacement by vascularized os pisiform transfer in advanced Kienb$\ddot{o}$ck's disease. Materials & Methods: There were two men and three women. The mean age was 55 years, ranging from 41 to 70. According to Lichtman's classification, three cases were stage IIIb and two cases were stage IV. Results: At the period of follow up, pain during motion was markedly diminished in all patients, the arc of motion ranged on average from 49 degrees of flexion to 53 degrees of extension and the grip power of the affected hand reached on average 83% compared with the contralateral side. Clinical results assessed by DASH and modified Mayo score showed 8 and 90. On plain X-rays, carpal height ratio and radioscaphoid angle were not changed postoperatively. Conclusion: Lunate excision and vascularized os pisiform transfer is a reliable alternative method for the treatment of advanced Kienb$\ddot{o}$ck's disease.

Histologic Findings and Inflammatory Reactions After Long-term Colonization of Helicobacter felis in C57BL/6 Mice

이주엽,김나영,최윤정,남령희,최윤진,권용환,Kichul Yoon,서지형,Seon Min Lee,이혜승,이동호 대한암예방학회 2014 Journal of cancer prevention Vol.19 No.3

Background: The Helicobacter felis (H. felis) mouse model has been developed for the research regarding pathogenesis of chronic gastritis and gastric cancer. The aim of this study was to investigate long-term H. felis colonization in the stomachs of C57BL/6 mice and subsequent histologic findings and inflammatory reactions including pro-inflammatory cytokines. Methods: Twenty-three female C57BL/6 mice at 4 weeks of age were gavaged with H. felis, and 13 control mice served as vehicle only. The mice were sacrificed at 4, 24, and 52 weeks after inoculation. The infection status and degree of inflammation were determined by culture and histopathology. The level of gastric mucosal myeloperoxidase (MPO), tumor necrosis factor alpha (TNF-α), and interleukin-1beta (IL-1β) were measured by ELISA. Results: The overall infection rate was 100%, as determined by the culture and histology. At 4, 24, and 52 weeks, the neutrophil and monocyte scores were significantly higher in infected mice than in control mice. At 24 weeks after inoculation, most of the infected mice showed mucosal atrophy with or without metaplasia, and a few showed focal dysplasia. Adenocarcinoma was observed in one mouse at 52 week post-infection. Gastric mucosal MPO and IL-1 levels were significantly higher in infected mice than those in control mice at 24 and 52 weeks. However, the expression of gastric mucosal TNF-α was not significantly different between the infected and control mice at any time-point. Conclusions: Long-term H. felis-infection in C57BL/6 mice provoked a severe inflammatory reaction and it progressed into atrophy, metaplasia, dysplasia and cancer. IL-1β might play an important role in the inflammatory response of mice to Helicobacter species.

화학물질 관리에 대한 이해 54 - 공정안전관리(PSM) 제도의 현황 및 개선방안

이주엽,Lee, Ju-Yeop 대한산업보건협회 2015 산업보건 Vol.332 No.-

Skin layer 코팅에 사용되는 Mono Methyl Ether 기반 수성 폴리우레탄의 제조 및 물리적 성질

이주엽,Lee, Joo-Youb 한국응용과학기술학회 2019 한국응용과학기술학회지 Vol.36 No.2

본 연구에서는 가죽 스킨층에 코팅용으로 사용할 수용성 폴리우레탄의 합성을 위해 polyrupopylene mono methyl ether (PM)를 기반으로 폴리 에테르 폴리올인 폴리 프로필렌 글리콜과 이소포론 디 이소시아네이트 (isophorone diisocyanate, IPDI)를 합성 하였다. 예비 중합체의 합성 후, 점도 상승을 억제하기 위해 $40^{\circ}C$에서 1M, 2M, 3M 및 4M으로 PM을 첨가하고 중화 반응 및 사슬연장 반응을 수행하여 폴리 우레탄 시료를 준비 하였다. 준비된 시료의 인장강도와 연신율, 접착강도를 측정한 결과로 인장강도는 PM 이 1M 일 때 2.109 kgf/mm2, 4M 에서 1.721kgf/mm2, 연신율은 PM이 1M일 때 496%, 4M 에서 522%, 접착력은 PM이 1M 일 때 1.114 kgf/cm, 4M 에서 0.99 kgf/cm 로 확인 되었다. In this study, polyether polyol polypropylene glycol and isophorone diisocyanate (IPDI) were synthesized based on polyrupopylene mono methyl eher (PM) for the synthesis of water - soluble polyurethane for coating on leather skin layer. After synthesis of prepolymer, PM was added at $40^{\circ}C$ to 1M, 2M, 3M, and 4M to inhibit the viscosity rise, and neutralization reaction and chain extension reaction were carried out to prepare polyurethane samples. According to the measurement results of the tensile strength, elongation and adhesive strength of the prepared sample, the tensile strength was 2.109 kgf / mm2 for PM 1M, 1.721kgf / mm2 for 4M, elongation was 496% for PM 1M, 522% for 4M, adhesion was 1.114 kgf / cm for PM 1M and 0.99 kgf / cm for 4M.

Correlations Among Endoscopic, Histologic and Serologic Diagnoses for the Assessment of Atrophic Gastritis

이주엽,김나영,이혜승,Jane C Oh,권용환,최윤진,Kichul Yoon,Jae Jin Hwang,Hyun Joo Lee,AeRa Lee,정연상,조현진,윤혁,신철민,박영수,이동호 대한암예방학회 2014 Journal of cancer prevention Vol.19 No.1

Atrophic gastritis is a precancerous condition, which can be diagnosed by several methods. However, there is no consensus for the standard method. The aim of this study was to evaluate the correlations among endoscopic, histologic, and serologic findings for the diagnosis of atrophic gastritis. Methods: From March 2003 to August 2013, a total of 2,558 subjects were enrolled. Endoscopic atrophic gastritis was graded by Kimura-Takemoto classification and histological atrophic gastritis was assessed by updated Sydney system. Serological assessment of atrophic gastritis was based on serum pepsinogen test. Results: The serum pepsinogen I/II ratio showed a significant decreasing nature when the extent of atrophy increased (R2=0.837, P<0.001) and the cut-off value for distinguishing between presence and absence of endoscopic atrophic gastritis was 3.2. The serum pepsinogen I and pepsinogen I/II ratio were significantly lower when the histological atrophic gastritis progressed and the cut-off value was 3.0 for a diagnosis of histological atrophic gastritis. A significant correlation between endoscopic and histological atrophic gastritis was noted and the sensitivity and specificity of endoscopic diagnosis were 65.9% and 58.0% for antrum, 71.3% and 53.7% for corpus, respectively. Conclusions: The endoscopic, histological, and serological atrophic gastritis showed relatively good correlations. However, as these three methods have a limitation, a multifactorial assessment might be needed to ameliorate the diagnostic accuracy of atrophic gastritis.

열매체유 시스템의 안전관리 개선방안

이주엽(Joo Yeob Lee),이형섭(Hyung Sub Lee),이근원(Keun-Won Lee) 한국가스학회 2013 한국가스학회 학술대회논문집 Vol.2013 No.5

열수송관 이상 탐지를 위한 멀티 모달 인공지능 모델

이민영(Minyoung Lee),이주엽(Juyeob Lee),김현지(Hyunji Kim),이진모(Jinmo Lee),박민수(Minsu Park),안채희(Chaehee An),임준우(Junwoo Lim),한승희(Seunghee Han),이홍철(Hongcheol Lee),황인주(Inju Hwang),김연호(Yeonho Kim),박은일(Eunil Park) 한국정보과학회 2022 한국정보과학회 학술발표논문집 Vol.2022 No.12

위점막 순응 및 소장 손상으로 인한 만성 비스테로이드성 소염제 유발 위염증 백서 모델 수립의 어려움

이병환 ( Byoung Hwan Lee ),김나영 ( Nayoung Kim ),남령희 ( Ryoung Hee Nam ),이주엽 ( Ju Yup Lee ),이혜승 ( Hye Seung Lee ),이창희 ( Chang Hee Lee ),박지현 ( Ji Hyun Park ),이동호 ( Dong Ho Lee ) 대한소화기학회 2014 대한소화기학회지 Vol.63 No.6

Background/Aims: The prevalence of peptic ulcer disease has not decreased mainly due to an increase in the use of NSAIDs. This study was conducted in order to determine whether a chronic NSAID-induced gastric inflammation model could be established by repeated administration of NSAID. Methods: Indomethacin (10 mg/kg) was administered once per week for six weeks in 8- and 26-week rats and animals were sacrificed every week after administration. Gross ulcer index, histologic damage index, myeloperoxidase (MPO) activity, and mucus (glucosamine) levels were measured. Small bowel damage was also evaluated. Results: Gross gastric damage index showed a peak level at three weeks and then decreased slowly in the 26-week indomethacin group. Gastric mucosal glucosamine level increased in both the 8-week (p=0.038) and 26-week groups (p=0.007). In addition, gastric mucosal MPO level decreased in the 8-week group (p=0.018) but did not show a decrease in the 26-week group. Small bowel damage began to occur at three weeks during the schedule and eight of 36 rats (22.2%) died due to perforation or peritonitis of the small bowel in the 8- and 26-week indomethacin groups, respectively. Conclusions: Due to gastric adaptation and small bowel damage, repeated administration of NSAID to experimental animals may not be an adequate method for establishment of the chronic gastric inflammation model. (Korean J Gastroenterol 2014;63:341-347)