Monensin 사료첨가가 한국재래산양의 제일위내(第一胃內) 미생물상과 그 활성에 미치는 영향

이은,이학철 한국낙농학회 1982 韓國酪農學會誌 Vol.4 No.3

The present experiments were conducted to determine the effect of feeding monensin on rumen bacterial flora and protozoal fauna, on bacterial enzyme activities involving amylase and urease, on ruminal pH and volatile fatty acids. Nine Korean native goats averaging 15.6 ㎏ of body weight were randomly alloted to the diets supplemented with monensin at the level of 0, 30 and 60 ppm. After 3 weeks adaptation period the animals were fed the experimental diets. Ruminal fluids were collected and examined just before feeding and 2, 4, 6 and 10 hrs after feeding on days 0, 7, 14, 28 and 42. The results obtained in this study are summerized as follows: 1. The total number of ruminal protozoa was markedly decreased with monensin diets from the 7th to 28 th day of the experimental period, although it appeared to be recovered gradually towards the end of experiments. However, a significant difference ( P< .05 ) among tereatments could be noted in the total number of ruminal protozoa throughout the experimental period. 2. A total of 20 protozoal species were detected throughout the period ; 17 species in Ophryoscolacidae, 3 species in Isotrichidae. The number of those was decreased by monensin diets, so that the protozal composition of monensin groups was simpler than that of the control. 3. The protozoal fluctuation pattern was changed by the monensin treatment, and the fluctuation of each species consisted of the protozoal composition was varied according to not only species, but also treatments. The counts of protozoa of Holotricha order were appeared to be increased by monensin treatments while no significant differences were noted in counts of protozoa of Spirotricha order except Eudiplodinium species shown slight increase in number. 4. In the total number of ruminal bacteria, nonsignificant difference ( P > .05 ) was observed among treatments throughout the experimental period. 5. Fourteen different kinds of the rumen bacterial types were classified by means of morphology and Green-stain reaction. In the total number of bacterial types, nonsignificant difference ( P>.05 ) was observed among treatments. 6. The bacterial fluctuation pattern was changed by the monensin treatment, and the fluctuation of each bacterial type consisted of the bacterial composition was varied according to bacterial types and monensin levels. The total numbers of Gram-negative bacteria increased (15.78-16.41 %) with monensin treatments, While, the numbers of Gram-positive bacteria decreased ( 69.65-73.09% ) throughout the experimental period. 7. Amylase activity in ruminal fluids of the monensin diet tend to be increased from the 7th to 28 th or 42nd day of the experimental period : although no significant difference in amylese activity was noted among treatments. 8. Urease activity in ruminal fluids of the 30 ppm diet appeared to be increased from the 7 th to 28th day of the experimental period, while the 60 ppm diet tend to be decreased from the 7th to the end of the experiment. However, no significant difference (P >.05) was observed between the 30 ppm and control group, and 60 ppm group was significantly different from the other two groups (P< .05). 9. In the ruminal pH, nonsignificant differences (P>.05) were found among treatments throughout the experimental period. 10. The total ruminal VFA concentration was found not to be affected by the monensin treatments. 11. The molar portion of acetate in rumen fluids were reduced ( P< .05 ) by monensin diets, propionate, however, increased ( P<.05 ) from the 7th to the end of the experiment. The butyrate was significantly decreased at the 60 ppm level ( P< .05 ). Nonsignificant difference ( P>.05)was observed in isovalerate and valerate.

Two Cases of Chloromethylisothiazolinone and Methylisothiazolinone-associated Toxic Lung Injury

이은,손승국,윤지선,조현주,양송이,정성수,도경현,조영아,이소연,박동욱,홍수종 대한의학회 2018 Journal of Korean medical science Vol.33 No.16

Previous animal studies have not conclusively determined the association between exposure to humidifier disinfectants (HDs) containing 5-chloro-2-methyl-4-isothiazolin-3-one (CMIT) and/or 2-methyl-4-isothiazolin-3-one (MIT) and development of HD-associated lung injuries. Nonetheless, patients exposed to HDs containing only CMIT and/or MIT showed clinically similar lung injuries to those exposed to HDs containing polyhexamethylene guanidine (PHMG) or oligo (2-[2-ethoxy]ethoxyethyl) guanidinium chloride (PGH). Here, we report twin sisters with lung injuries associated with exposure to CMIT/MIT-containing HDs. At 6 months of age, a younger twin sister presented with the 3-day history of cough, sputum, and respiratory difficulty. Chest radiography revealed multiple patchy consolidation and ground-glass opacities with pneumothorax and pneumomediastinum. Thoracostomy was performed due to pneumothorax at admission and she was discharged at 11 days of hospitalization. At 5 years of age, multiple tiny nodules and faint centrilobular ground-glass opacities were observed with the small pneumatocele. The elder sister visited a tertiary hospital due to dyspnea at 12 months of age. Chest radiography showed consolidation, pneumomediastinum, and pulmonary interstitial emphysema. There was no response to the administration of immunosuppressant drugs and antifibrotic agents. At 5 years of age, chest CT revealed ground-glass opacity and multiple tiny centrilobular ground-glass opacities nodules in both lungs with exercise intolerance.

씀바귀의 지질강하 및 항산화효과

이은 한국자원식물학회 2011 한국자원식물학회지 Vol.24 No.1

To investigate effects of Ixeris dentata EtOH ext. on lowering lipid levels and anti-oxidation activity, hyperlipidemic rats were treated with Ixeris dentata EtOH ext. and lipid levels and anti-oxdation activity were measured. The Ixeris dentata EtOH ext. groups showed low concentration of plasma FFA, plasma triglyceride, plasma total cholesterol, and plasma LDL-cholesterol compared to control group. However, concentration of plasma HDL- cholesterol was not significantly different among all the treatment groups. The Ixeris dentata EtOH ext. groups showed lower level of liver total cholesterol, liver triglyceride, plasma TBARS, and liver TBARS than those of control group. The Ixeris dentata EtOH ext. groups also showed higher level of GSH-Px activity, SOD activity, and CAT activity than those of control group. Moreover, the Ext. showed lower level of TNF-α, Apo-B, Apo-E, and leptin expression than those of control group. The results in this study shows that the Ixeris dentata EtOH ext. have positive effect in lowering lipid level, and anti-oxidative activity. 씀바귀추출물이 비만을 유도한 흰쥐의 지질강하 및 항산화 효과에 미치는 영향을 검토하였다. 혈장 유리지방산과 triglyceride농도는 대조군과 비교하여 씀바귀추출물 투여군은 농도 의존적으로 낮은 값을 나타내었다. 혈장 내 total cholesterol 농도와 LDL-cholesterol 농도는 씀바귀추출물 투여군 모두에서 감소하는 경향을 보였다. 그러나, HDL-cholesterol농도는 처리군 들 간에 유의한 차이를 나타내지 않았다. 간장 total cholesterol과 triglyceride량은 씀바귀추출물 투여군 들이 대조군보다 낮은 값을 나타내었다. 혈장 및 간장의 TBARS 농도는 씀바귀추출물 투여군 들 모두가 대조군보다 유의하게 낮은 값을 나타내었다. GSH-Px, SOD 및 CAT의 활성치는 씀바귀추출물 투여군 들이 대조군보다 높은 값을 나타내었다. TNF-α, Apo-B, Apo-E 및 Leptin의 gene expression은 씀바귀추출물 투여군이 대조군보다 낮은 expression을 나타내었다. β-actin expression에 대한 TNF-α, Apo-B, Apo-E 및 Leptin의 gene expression의 비율은 TNF-α와 Apo-E는 대조군보다 씀바귀추출물 투여군 들이 낮은 경향을 보였으나, Apo-B 및 Leptin의 비율은 대조군을 비롯한 씀바귀추출물 투여군 들 간에 유의한 차이를 나타내지는 않았다. 이상의 결과들을 종합해보면, 씀바귀 추출물은 지질강하 및 항산화에 긍정적인 효과를 나타내었음을 시사해준다.

용융탄산염 연료전지용 리튬첨가 Ni-Al 합금 연료극의 제조 및 특성

이은,윤성필,한종희,남석우,임태훈,홍성안,이호인 한국공업화학회 2004 한국공업화학회 연구논문 초록집 Vol.2004 No.1

극점 외 1편

이은 고요아침 2020 열린시학 Vol.25 No.3

Stereoselective Synthesis of (-)-Centrolobine

이은,김학중,장원석 대한화학회 2004 Bulletin of the Korean Chemical Society Vol.25 No.11

삼황사심탕약침의 지질강하 및 항산화효과

이은,이준무,Lee, Eun,Lee, Joon-Moo 대한약침학회 2005 Journal of pharmacopuncture Vol.8 No.1

Effects of Samhwangsasimtang aqua-acupuncture at gansoo($B_{18}$) and chungwan($CV_{12}$) on antihyperlipidemic and antioxidant were investigated in rat fed high fat diet. Concentrations of triglyceride, free fatty acids, lipoprotein and glucose in plasma showed a tendency to decrease in the aqua-acupuncture groups. In plasma lipid composition, total cholesterol and LDL- cholesterol showed a tendency to decrease in aqua-acupuncture groups, however the values of HDL-cholesterol showed no significantly different in the treatment groups. Concentration of thiobarbituric acid reactive substances(TBARS) in plasma and liver showed a low in the aqua-acupuncture groups. The values of glutathione peroxidase(GSH-Px) and catalase(CAT) activity showed a tendency to increase in aqua-acupuncture groups. However the values of superoxide dismutase(SOD) activity showed no significantly different in the treatment groups.

[동화 부문] 그냥시계방

이은 아동문학평론사 2015 아동문학평론 Vol.40 No.4

남해안지역 신석기시대 생업활동 연구 - 패총 출토 유물을 중심으로 -

이은 한국신석기학회 2016 한국신석기연구 Vol.- No.31

This paper was to find out the aspects of livelihood activities in the Southern coastal area during Neolithic Era through animal remains excavated from shell mound relics. First, we reviewed the Neolithic Era environment and distribution of the ruins and analyzed relics by dividing artifacts associated with livelihood activities excavated from ruins and animal remains into stone tools, bone tools and shellfish, fish, mammals, birds, respectively. As a result, various univalve shells, mussel and pacific oyster account for the majority in shellfish. Fishing activities were carried out by combined fishing and harpoons and fish excavated from Southern coastal area are mainly red sea-breams and various breams. Mammals are mainly sea lions of marine mammals and deer and wild pigs which are land mammals and can be seen to have been used as the main food resource and this is the same as the mammal aspect in the Korean peninsula during the Neolithic Era. It can be also seen that fishing activities were actively carried out in the southern coast where Yeoseodo Shell Mound, Ando Shell Mound and Dongsam-dong Shell Mound are located and there were exchanges with Jeju Island and European Region through the sea.

이은의 변호사 : 실천적인 성 담론이 필요하다

이은의,지승호,권영탕(사진) 인물과사상사 2018 인물과 사상 Vol.- No.238