보정방독탕 에탄올 추출물의 안전성에 대한 연구

이은옥,이효정,이효정,정수진,최도영,정희재,안규석,강종구,김성훈,Lee, Eun-Ok,Lee, Hyo-Jeong,Lee, Hyo-Jung,Jeong, Soo-Jin,Choi, Do Young,Jung, Hee-Jae,Ahn, Kyoo-Seok,Kang, Jong-Gu,Kim, Sung-Hoon 대한암한의학회 2010 大韓癌韓醫學會誌 Vol.15 No.1

Bojungbangdocktang (BJBDT), a formula of eight Oriental herbs, is a modified herbal prescription of Bangdoktang and Bojungbangamtang. Recently, BJBDT was demonstrated to inhibit angiogenesis induced by vascular endothelial growth factor in human umbilical vein endothelial cells, enhance hematopoiesis and protect cisplatin-induced cytotoxicity in normal MCF-10A breast cells. Nevertheless, there is no safety study of BJBDT before clinical trial so far. Thus, in the current study, we investigated the toxicity about ethanol-extracted BJBDT. Male and female Spraque Dawley (SD) rats were given orally by BJBDT at 250, 500, and 1000 mg/kg for 4 weeks. Mortality, clinical signs and measured change of body weight, food consumption and water consumption were observed. In addition, we performed ophthalmologic, urinary, hematological, blood serum biochemical and histopathological examination. Any general toxicity was not found in BJBDT treated group. Also, there were no significant differences in the parameters such as body weight, food consumption and water consumption, a lot of urine and blood factor levels except HCT, MCHC, Ca, TG, Glucose and T-Bilirubin level compared with control group. Although HCT was elevated and TG was decreased in male rats, and MCHC, Glucose and T-Bilirubin were elevated and Ca and HCT were decreased in female rats, these were within normal ranges. Finally, we determined that maximum tolerated dose (MTD) was 1000 mg/kg and no observed adverse effect level (NOAEL) was 500 mg/kg. Taken together, these results demonstrated that BJBDT is very safe to SD rats.

RF UBM Sputtering에 의해 증착된 hBN 박막의 미세구조가 cBN 상의 핵형성에 미치는 영향

이은옥,박종극,임대순,백영준,Lee Eun-Ok,Park Jong-Keuk,Lim Dae-Soon,Baik Young-Joon 한국진공학회 2004 Applied Science and Convergence Technology Vol.13 No.4

Si(100) 기판 위에 RF UBM 스퍼터링 (Unbalanced Magnetron Sputtering) 방법을 이용하여 BN 박막을 증착하였다. 이온 충돌 에너지에 영향을 주는 증착 압력과 기판 바이어스 전압을 변화시켜, 증착된 BN박막의 미세구조와 압축응력의 변화를 살펴보았다. 높은 증착 압력에서는 hBN laminate의 정렬도가 기판 바이어스 전압이 증가함에 따라 선형적으로 증가한 반면, 낮은 증착 압력에서는 낮은 기판 바이어스 전압에서 hBN laminate의 정렬도가 높게 나타났다. hBN 박막의 응력 변화와 표면 형상은 hBN laminate의 정렬도와 밀접한 관계가 있는 것으로 관찰되었는데, 이의 적절한 조절에 의해 압축응력의 증가 없이도 hBN 박막 위에 cBN 상의 핵 형성이 일어날 수 있었다. Boron nitride thin films were deposited on Si(100) substrate by RF (Radio-frequency) UBM (Unbalanced Magnetron) sputtering system. The effect of working pressure and substrate bias voltage on microstructure and compressive stress of boron nitride thin films has been investigated. In high working pressure, the alignment of hBN laminates increased with substrate bias voltage, in low working pressure, however, it was high in low substrate bias voltage. Compressive stress evolution and surface morphology of deposited BN films are closely related with the alignment of hBN laminates. The cBN phase without high compressive stress could be nucleated on hBN thin film by controlling the alignment of hBN laminates.

반지련으로 부터 분리한 luteolin의 세포고사효과

이은옥,김진형,안규석,박영두,김성훈,Lee, Eun-Ok,Kim, Jin-Hyung,Ahn, Kyoo-Seok,Park, Young-Doo,Kim, Sung-Hoon 대한동의생리학회 2005 동의생리병리학회지 Vol.19 No.3

We previously demonstrated that the methylene chloride fraction of Scutellaria barbata suppessed human leukemic U937 cell proliferation by inducing apoptosis. In the present study, we have isolated luteolin from Scutellaria barbata and evaluated its apoptotic mechanism in Lewis lung carcinoma cells. Luteolin inhibited the proliferation of Lewis lung carcinoma cells in a concentration-dependent manner. Luteolin effectively increased the portion of $sub-G_1$ DNA content (apoptotic portion) and apoptotic Annexin-V positive cells in a concentration-dependent manner by FACS analysis. Caspase 9 and caspase 3 were activated and PARP was effectively cleaved by luteolin. It also increased the ratio of Bax to Bcl-2 through the decrease of Bcl-2 expression by Western blotting and reduced mitochondrial membrane potential following TMRE staining. These results suggest that luteolin can induce apoptosis through the mitochondrial mediated pathway.

Immunoglobulin G(IgG)를 함유한 DOPE 리포솜의 제조와 담즙산염에 의한 용해 특성

이은옥,김진구,김종득,Lee, Eun-Ok,Kim, Jin-Gu,Kim, Jong-Duk 한국약제학회 1990 Journal of Pharmaceutical Investigation Vol.20 No.3

The effects of bile salts (BS) on the stability of dioleoylphosphatidylethanolamine (DOPE) liposomes were investigated, observing apparent absorbance of vacant liposomes and calcein release from entrapped liposomes. Unilamellar liposomes were prepared by using a small quantity of palmitoly-immunoglobulin G(IgG) ($2.5{\times}10^{-4}$ mo1/lipid mol) to stabilize the bilayer phase of the unsaturated DOPE which by itself does not form stable liposomes. The destabilization of PE immunoliposomes by papain, clearly demonstrates that the IgG is essential for stabilization of PE bilayer. Approximately 4% of the entrapped calcein was released from the PE liposomes after 1 hr from liposome formation. Calcein release and absorbance of liposomes depended on the BS/lipid ratio because of the solubilization of lipid molecule in bilayer and the formation of mixed micelles. At very low BS concentrations, the incorporation of BS induced BS/lipid aggregates in the outer vesicles monolayer, while high BS concentrations, mixed micelles were formed. Chelate and its conjugates as $3{\alpha},\;7{\alpha},\;12{\alpha}-trihydroxy$ BS induce the concentration of the $3{\alpha}$, $12{\alpha}-dihydroxy$ BS at half-maximal solubilization of immunoliposomes to approximately 2.5-, or 5-fold. Conjugation of BS with glycine or taurine slightly enhanced their capacities to perturb membranes.

통증 관리에 대한 의사와 간호사의 지식과 태도 비교

이은옥,허대석,김순자,김열홍,윤성수,권인각,조석구,박명희,박정윤,Lee, Eun-Ok,Heo, Dae-Seok,Kim, Soon-Ja,Kim, Yeul-Hong,Yoon, Sung-Soo,Kwon, In-Gak,Cho, Seok-Goo,Park, Myung-Hee,Park, Jung-Yoon 한국호스피스완화의료학회 1999 한국호스피스.완화의료학회지 Vol.2 No.1

목적 : 암환자에게 있어 통증은 삶의 질을 좌우하는 가장 중요한 증상이다. 현재의 통증 치료 방법 즉, 적절한 약물 치료 방사선, 마취, 신경차단 등의 방법으로 97%정도가 통증 조절이 가능함에도 불구하고, 여러 국내외 문헌에서는 암환자의 $60{\sim}70%$가 통증을 호소하고 있음을 보고하고 있다. 이에 이 연구에서는 의료진 측면에서 적절한 통증 치료의 장애가 되는 요인을 찾고, 진통제 및 암환자의 통증에 대한 잘못된 인식과 편견을 파악하여 보다 효과적인 교육프로그램을 개발하는 기초 조사를 마련하고자 한다. 방법 : 1998년 5월 1일부터 5월 31일까지 한달 동안 서울 시내에 있는 네개의 종합병원 내과에서 근무하고 있는 의사와 간호사를 대상으로 통증 관리에 대한 지식과 태도에 관한 도구를 수정한 32개 문항의 설문지를 통해 조사하였다. 설문의 내용은 통증 사정이 6문항, 진통제 투약이 5문항, 진통제 약물 작용이 8문항, 진통제 분류가 11문항, 그리고 진통제 투약 태도 2문항으로 이루어졌다. 결과 : 의사가 99명, 간호사가 152명이 참여하였으며, 전체 정답율은 의사가 71.6%, 간호사가 70.3%였다. 그러나, 그 중 진통제 분류에서는 80% 이상의 정답율을 보였으나 나머지에서는 대부분 $50{\sim}60%$정도의 정답율을 보이면서 통증 관리에 대한 낮은 지식 정도를 보여 주었다. 통증 사정에서는 간호사의 지식이 유의하게 높았으며, 진통제의 약리작용과 진통제 분류에서는 의사의 지식이 유의하게 높았다. 그러나, 전체 통증 관리에 대한 지식과 태도에 있어 의사 평균이 21.40이었으며, 간호사의 평균은 20.87로서 유의한 차이를 보이지 않았다. 결론 : 암환자 통증 관리에 대해 의료진의 지식 정도이 부족함이 나타났고, 이에 대한 적극적인 대안으로 의사와 간호사를 위한 교육프로그램 개발과 교육이 절실히 필요한데 일시적이고 일회적인 교육보다는 지속적이고 장기적이고 자동적으로 운영되는 체계가 필요하다. This survey was designed to evaluate knowledge and attitudes of physicians and nurses toward cancer pain management in South Korea and to compare physicians' knowledge and attitudes with nurses' Ninety-nine physicians and 152 nurses working at four major institutions in South Korea were included for the study. With the 30 items of the knowledge with true and false answers about cancer pain such as pain assessment(6 items), pharmacokinetics of opioids(8 items), analgesics classification(11 items), and drug administration(5 items), total score of knowledge answered by physicians was 21.40, which was not significantly higher than 20.87 answered by nurses. Rates of the correct answer were more than 70% in both physicians and nurses. Physicians were more knowledgeable in pharmacokinetics and analgesics classification than nurses, while nurses higher only in pain assessment than physicians. Since physicians and nurses could not effectively manage the cancer pain because of inappropriate knowledge, it is important to provide intensive education to physicians and nurses about cancer pain management.

환자의 수술후 경과시간에 따른 동통정도의 변화에 관한 연구

이은옥(Eun-ok Lee),김수진(Soo Jin Kim),이경숙(Kyoung Sook Lee) 한국간호과학회 1984 Journal of Korean Academy of Nursing Vol.14 No.1

고밀도 폴리에틸렌의 표면결정성과 그 산화속도

이은옥(Eun Ok Lee),김정엽(Chung Yup Kim) 한국고분자학회 1980 폴리머 Vol.4 No.5

4개의 송신 안테나를 사용하는 S-T 방식의 Rayleigh fading에서의 성능

이은옥(Eun Ok Lee),이혁재(Hyuck Jae Lee) 대한전자공학회 2002 대한전자공학회 학술대회 Vol.2002 No.6

통증어휘를 이용한 통증비율척도의 개발연구

이은옥(Lee Eun-Ok),윤순녕(Yun Soon Nyong),송미순(Song Misoon) 한국간호과학회 1984 Journal of Korean Academy of Nursing Vol.14 No.2

기독교 예복에 관한 기독교인의 의식 조사

이은옥(Eun Ok Lee),이현정(Hyun Jeong Lee),오경화(Kyung Wha Oh) 한국복식학회 2003 服飾 Vol.53 No.3

This study is to clarify the true meaning of christian ceremonial dress, based on the Biblical background, by examining the origin and the symbolic meaning of the Catholic ceremonial dress. The influence of the Liturgical movement on the ceremonial dress and the changes in the symbolic meaning of the Christian ceremonial dress are studied. This research also aims to closely examine the difference in Christians` consciousness on the Christian ceremonial dress such as gown, stole, and clergy shirt. It aims to provide basic resources for the education and further study on the Christian ceremonial dress through reconfirming the basic understanding and the symbolic meaning of the Christian ceremonial dress. As a result, according to the lesson of the Bible and the ideology of the Reformation, it is suggested that priests should wear regular suits or the traditional Hanbok rather than wearing gown or Roman collar. However, if most of Christians believe that it is critical to wear ceremonial dress to project holiness and seriousness during the service, it is suggested that priest should wear gown only and avoid Roman collar and stole because they only show the hierarchy of priests. Accordingly, there is a hope in this research that the knowledge of ceremonial dress could be re-defined based on the Evangelism in the Bible though the results of the study.