사구체 상피세포와 혈관사이세포 내에 안지오텐신 II와 레닌-안지오텐신계 구성인자들의 공존

이원정,고철우,박매자 대한해부학회 2002 Anatomy & Cell Biology Vol.35 No.2

환경보건적 요소가 도시 내 폭염 취약성 평가 결과에 미치는 영향 분석

이원정,강재은,김유근,Lee, Won-Jung,Kang, Jae-Eun,Kim, Yoo-Keun 한국환경보건학회 2013 한국환경보건학회지 Vol.39 No.6

Objectives: This analysis seeks to evaluate the impact of environmental health factors (EHF; e.g. hospital beds per capita, employees of medical institutions) on extreme-heat vulnerability assessment in Busan Metropolitan City during 2006-2010. Methods: According to the vulnerability concept suggested by the Intergovernmental Panel on Climate Change (IPCC), extreme-heat vulnerability is comprised of the categories of Exposure, Sensitivity, and Adaptive Capacity (including EHF). The indexes of the Exposure and Sensitivity categories indicate positive effects, while the Adaptive capacity index indicates a negative effect on extreme-heat vulnerability. Variables of each category were standardized by the re-scaling method, and then each regional relative vulnerability was computed with the vulnerability index calculation formula. Results: The extreme-heat vulnerability index (EVI) excepting EHF was much higher in urban areas than in suburban areas within the metropolitan area. When EHF was considered, the difference in the EVI between the two areas was reduced due to the increase of the Adaptive capacity index in urban areas. The low EVI in suburban areas was induced by a dominant effect of natural environmental factors (e.g. green area) within the Adaptive capacity category. Conclusions: To reduce the vulnerability to extreme heat in urban areas, which were more frequently exposed to extreme heat than others areas, public health and natural environments need to be improved in sensitive areas.

다중 퍼셉트론 기법을 활용한 철도차량 견인용 영구자석 동기 전동기의 영구자석 형상 설계 유효성 검증

이원정,이재범,이형우,임재현,김충서,홍석민,이찬규,백종민,박찬배 한국철도학회 2023 한국철도학회 학술발표대회논문집 Vol.2023 No.11

CIP조건의 국제물품매매계약에서 항공화물에 대한 피보험이익 - 대법원 2018.3.15. 선고 2017다240496판결을 중심으로 -

이원정 한국해법학회 2020 韓國海法學會誌 Vol.42 No.2

CIP조건상 매도인은 합의된 장소에서 자신이 지명한 운송인에게 물품을 인도하고, 지정목적지까지 물품의 이동을 위한 운송계약을 체결해야 하며, 특히 운송 중 물품의 멸실 또는 손상에 대한 매수인의 위험을 담보하는 보험계약을 체결해야 한다. 그런데 보험계약상 보험자로부터 보상이 이루어지기 위해서는 피보험자가 손해발생시점에서 보험목적물에 대하여 피보험이익을 가져야만 한다. 2018년 우리나라 대법원의 「2017다240496판결」에서는, CIP조건상 매도인이 매수인의 위험부담 구간인 항공운송 중에 발생된 물품의 손해에 대하여 피보험자로서 보험금을 청구할 수 있는지가 쟁점이 되었다. 대법원은 매도인이 보험자로부터 보상을 받을 수 있는 적하보험증권상 피보험자이며, 물품이 목적지에 손상 없이 도착할 때까지 물품의 소유자이자 기대이익을 갖는 자로서 피보험이익을 갖는다고 판시하였다. 이번 판결은 CIP조건에 따라 매수인을 위해 매도인이 체결한 항공적하보험계약상 매도인의 법적 지위에 관한 문제를 최초로 다루었다는 점에서 중요한 의미가 있다. 따라서 본 논문은 대법원의 판결이유를 분석하고, 상법상 타인을 위한 보험의 법리, 협회적하약관(항공)의 관련규정, CIP조건의 법적 성질, 항공화물운송장의 발행 관행 등을 기초로 판결의 타당성을 평가한다. Under CIP Term, the seller delivers the goods to the carrier nominated by him at an agreed place, and must contract for carriage necessary to bring the goods to the named place of destination, and also should contract for insurance cover against the buyer’s risks of loss of or damage to the goods during the carriage. It should be noted that under the insurance contract, in order to recover from the insurer, the assured must have an insurable interest in the subject-matter insured at the time of loss. In 2018, a decision(2017Da240496) as to whether the seller as the assured, can claim insurance money against the insurer for loss of goods during air transit which is the buyer’s risk was handed down by the Korean Supreme Court. The Supreme Court decided that the seller was the assured under cargo insurance policy to recover loss of goods from the insurer, and had also an insurable interest as the owner of the goods as well as the holder of expected profit, until the goods arrived at the destination safely. This case has important implication to academic world as well as business world, because it was the first case in Korea which handled the issue as to the seller’s legal position under the contract for air cargo insurance. Therefore, this article attempts to analyze the reasoning behind the Supreme Court's decision, and evaluates the appropriateness of the court's decision on the basis of the legal principle on insurance for benefits of third party under Korean Commercial Act, relevant provisions of Institute Cargo Clause(Air), legal nature of CIP term, practice of Air waybill’s issuance.

Differences in Proportion of N-acetyllactosamine and O-acetylated Sialic Acid Have No Significant Effect on the Pharmacokinetics and Biological Activity of Darbepoetin Alfa

이원정,오후근,최인성,이근호,신현우,이윤정,박지혜,양유희,하경식,이동억,최강열,최은영 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2

Darbepoetin alfa is used to treat anemia in patients with chronic renal failure and is a therapeutic glycoprotein with five N-glycosylation sites and one Oglycosylation site. Since the glycosylation of therapeutic proteins is known to affect pharmacokinetics and biological activity, it was wondered how the certain glycosylation structure of darbepoetin alfa affects pharmacokinetics and biological activity. To investigate the effects of glycosylation structures, several darbepoetin alfa samples were generated through the processes slightly modified from HK-DPO production process. Analysis of N-linked glycosylation profiles showed that darbepoetin alfa samples have different proportions of N-acetyllactosamine and O-acetylated sialic acids. Since these structures were known to affect the stability and efficacy of glycoproteins, we evaluated the pharmacokinetics and biological activities of darbepoetin alfa samples. As a result of pharmacokinetics, the time course of the concentration in plasma was similar to that of NESP®, commercially available darbepoetin alfa. Also, the relative AUClast ratio (sample/reference) was ranged from 84% to 114%, which indicated they did not have meaningful differences. For in vitro biological activity, a cell proliferation test was conducted in the EPO-dependent F36E cell-line. An in vivo biological activity test was performed with EP-based assay using B6D2F1 female mice. As a result, the biological activities of each samples were similar to those of reference drug, NESP®. In conclusion, different proportions of N-acetyllactosamine and O-acetylation of sialic acid in darbepoetin alfa did not show any meaningful effects on pharmacokinetics and biological activities.

치료 방사선량이 쥐의 난포 퇴축에 미치는 영향

이원정,선종률,유세종,안봉선 대한방사선방어학회 2012 방사선방어학회지 Vol.37 No.4

덩굴쪼김병 저항성 멜론을 위한 효율적이고 간편한 대량 검정법 개발

이원정,장경수,최용호,김흥태,김진철,최경자 한국식물병리학회 2015 식물병연구 Vol.21 No.3

This study was conducted to establish a simple mass-screening method for resistant melon to Fusarium wilt caused by Fusarium oxysporum f. sp. melonis (FOM). Root-dipping inoculation method has been used to investigate resistance of melon plants to Fusarium wilt. However, the inoculation method requires a lot of labor and time because of complicate procedure. To develop a simple screening method on melon Fusarium wilt, occurrence of Fusarium wilt on susceptible and resistant cultivars of melon according to inoculation method including root-dipping, soil-drenching, tip, and scalpel methods was investigated. Scalpel and tip methods showed more clear resistant and susceptible responses in the melon cultivars than root-dipping inoculation method, but tip method represented slightly variable disease severity. In contrast, in the case of soil-drenching inoculation method, disease severity of the susceptible cultivars was very low. Thus we selected scalpel method as inoculation method of a simple screening method for melon Fusarium wilt. By using the scalpel inoculation method, resistance degrees of the cultivars according to incubation temperature after inoculation (25 and 30oC) and inoculum concentration (1×106 and 1×107 conidia/ml) were measured. The resistance or susceptibility of the cultivars was hardly affected by all the tested conditions. To look into the effectiveness of scalpel inoculation methods, resistance of 22 commercial melon cultivars to FOM was compare with root-dipping inoculation method. When the melon cultivars were inoculated by scalpel method, resistance responses of all the tested cultivars were clearly distinguished as by root-dipping method. Taken together, we suggest that an efficient simple mass-screening method for resistant melon plant to Fusarium wilt is to sow the seeds of melon in a pot (70 ml of soil) and to grow the seedlings in a greenhouse (25±5oC) for 7 days, to cut the root of seedlings with a scalpel and then pour a 10 ml-aliquot of the spore suspension of 1×106 conidia/ml on soil. The infected plants were cultivated in a growth room at 25 to 30oC for about 3 weeks with 12-hr light a day. 본 연구는 Fusarium oxysporum f. sp. melonis에 의해 발생하는 멜론 덩굴쪼김병의 간편 대량 저항성 검정법을 확립하기 위하여 수행하였다. 멜론의 덩굴쪼김병 저항성 검정에는 대부분뿌리 침지(root-dipping) 접종 방법을 사용하고 있지만, 이 방법은 접종과정이 번거로워 노동력과 시간이 많이 소요된다. 간편저항성 검정법을 개발하기 위해 뿌리 침지, scalpel, tip 및 토양관주 방법으로 F. oxysporum f. sp. melonis 균주를 감수성 및 저항성 멜론 품종에 접종하여 덩굴쪼김병 발생을 조사하였다. 그결과 멜론 품종들은 scalpel 방법과 tip 방법에서 뿌리 침지 방법에서와 같은 분명한 저항성과 감수성 반응을 보였다. 하지만tip 방법은 scalpel 방법보다 개체간의 발병도 차이가 약간 더심하였으며 토양 관주 방법의 경우에는 감수성 멜론 품종들에서 덩굴쪼김병 발생이 매우 낮았다. 따라서 멜론 덩굴쪼김병의간편 대량 저항성 검정을 위한 효율적인 접종 방법으로 scalpel 방법을 선발하였다. 그리고 scalpel 방법으로 접종할 때 접종 농도(1×106, 1×107 conidia/ml)와 접종 후 재배 온도(25, 30oC) 에 따른 멜론 품종들의 덩굴쪼김병 발생을 조사한 결과, 이들은 scalpel 방법으로 접종된 멜론 품종의 저항성 정도에 거의 영향을 미치지 않았다. 그리고 확립한 간편 검정법을 이용하여 시판 멜론 22개 품종의 덩굴쪼김병 저항성을 뿌리 침지 접종 방법과 비교하여 실험하여 이 방법의 효용성을 확인하였다. 이와 같은 결과로부터 멜론 덩굴쪼김병에 대한 간편 대량 저항성 검정법으로 멜론 종자를 파종하고 온실(25±5oC)에서 7일 동안 재배한 유묘의 뿌리를 scalpel을 이용하여 상처를 준 후에 1×106 conidia/ml 농도의 멜론 덩굴쪼김병균 포자현탁액을 포트 당10 ml씩 관주하고 25–30oC에서 약 3주일 동안 재배하는 것을제안하고자 한다.

평택 칠원동 지구단위계획부지(3-2단계) 내 유적

이원정 중부고고학회 2019 중부고고학회 학술대회논문집 Vol.2019 No.3

반선지연으로 인한 손해배상액의 산정 - The Achilleas사건에서 영국 최고법원 판결을 중심으로

이원정 해상보험법연구회 2010 해상보험법연구 Vol.6 No.-

흰쥐 난포에서 저선량 방사선조사로 유도된 hormesis효과

이원정,손성경,이민아,양윤석,김원식 대한체질인류학회 2007 해부·생물인류학 (Anat Biol Anthropol) Vol.20 No.3

Hormesis효과는 생물체가 자연속에 존재하는 정도의 저선량 방사선 또는 독성물질 등에 노출된 후 단기 간 내에 나타나는 이로운 영향을 말한다. 저자들은 흰쥐 난포에서 저선량의 방사선을 조사한 후 고선량을 조사하 여 hormesis효과를 알아보고자 하였다. 본 실험에서는 4주령의 암컷 흰쥐(Sprague-Dawley계통)를 대조군, 2 cGy조사군, 2 cGy 조사후 2Gy조사군 및 2 Gy조사군으로 나누고, 각 군별로 단회 방사선 조사후 24시간에 희생시켜 난소를 적출하고 통상적인 방법으로 처 리한 후 H & E 염색과 TUNEL 면역염색을 실시하였다. 광학현미경으로 각 군 사이에 폐쇄난포의 비율과 난포의 발육단계별 정상난포의 비율을 비교하였다. 그 결과 난소내 전체 난포에서 차지하는 폐쇄난포의 비율은 2 Gy조사군(55.35 %)에서 2 cGy+2 Gy조사군 (39.27 %)에 비하여 현저히 증가하였으며, 난포의 발육단계에 따른 정상난포의 비율은 2 Gy조사군(일차난포, 82.15 %; 난포동형성전난포, 63.48 %; 난포동 난포, 21.58 %) 보다 2 cGy+2Gy조사군(일차난포, 94.37 %; 난포동형 성전난포, 78.81 %; 난포동 난포, 33.09 %)에서 모든 발육단계에서 유의하게 증가 하였다. 이같은 결과는 저선량의 방사선에 의해 난포발육단계에 hormesis효과가 나타났음을 의미하는 것으로 앞으로 방사선 hormesis연구에 있어 한 모델로 사용할 수 있을 것으로 사료된다. Hormesis is the generally-favorable biological responses to low exposures to toxins and other stressors. Radiation hormesis is the theory that ionizing radiation is benign at low levels of exposure, and that doses at the level of natural background radiation can be beneficial. The purpose of this study is to reveal the hormetic effect of low-dose radiation of ionizing radiation on the ovarian follicles of 4-week old female mice. Mice were grouped into control group, 2 cGy irradiated group, 2 cGy and 2 Gy irradiated group (2 cGY pre-exposure group), and 2 Gy irradited group. Mice were sacrificed by cervical dislocation 24 hours after irradiation, removed ovaries, fixed in neutral formaldehyde solution for 24 hours, embedded with paraffin, stained with hematoxylin and eosin and TUNEL immunohistochemically, and observed light microscopically the atretic follicles and normal follicles in various follicular developmental stages. In this experiment, the ratrio of atretic follicles to entire follicles in an ovary increased significantly in 2 Gyirradiated group compared with 2 cGY pre-exposure group, and the ratio of normal follicles to the entire follicles in an ovary in all the developmental stages were increased significantly in the 2 cGY pre-exposure group compared with 2 Gy-irradiated group. These results mean that low-dose radiation pre-exposure can induce the hormetic effect in the developing ovarian follicle.