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이용세,황병국 한국균학회 1982 韓國菌學會誌 Vol.10 No.4
Immature apple fruits of cultivars Fuji and Miller were completely resistant to Macrophoma sp. until 10 July. When inoculated on 31 July, apples became susceptible to this fungus, irrespective of cultivar. This fungus grew better on Fuji than Miller. Lesion enlargement on apple at 25℃ was remarkably pronounced relative to 20℃. In both cultivars, the amount of total soluble carbohydrates or reducing sugars in apple fruit flesh was gradually increased as apples became mature. The healthy apples of cultivar Fuji had a higher amount of carbohydrates than those of Miller. The infected apples contained more soluble carbohydrates in comparison to the healthy ones. In healthy apples, there were no consistent differences between Fuji and Miller in amount of total soluble amino acids. With increasing age of apples, the amount of soluble amino acids declined in the fruit flesh of both cultivars. Levels of carbohydrates and amino acids in apple fruits were discussed in association with the immature fruit resistance to Macrophoma sp.
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보리흰가루병(Erysiphe graminis DC f.sp. hordei Marchal)에 대한 길항균의 분리
이용세 대구대학교 농업과학연구소 1992 農業科學硏究論文集 Vol.6 No.-
Antibiotic-producing microorganisms (57 actinomycetes and 18 bacteria) were tested in vivo for effects on colony production of powdery mildew (Erysiphe graminis DC f. sp. hordei Marchal) on the leaf surface of the spring barley cultivar golden promise. Complete disease suppressions up to 100% were noticed by 13 strains out of actinomycetes. However, less than 50% disease suppression was appeared by 18 bacteria strains tested. In order to selection of the most effective strains, 13 actinomycetes were further tested. As results, actinomycetes, A 171 and A 252, showed remarkable inhibitory effects on the suppression of colony production of E. graminis on the leaf surface of spring barley cultivar golden promise. Two strains also demonstrated protective and curative effects against to the colony production of E. graminis on spring cultivar golden promise.
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길항균 Streptomyces sp. A 171 균주의 항균효과 및 배양적 특성
이용세 大邱大學校出版部 (대구대학, 한사대학 1992 農業科學硏究論文集 Vol.6 No.-
The powerful antagonistic actinomycetes, strain A 171, to powdery mildew(Erysiphe graminis DC f.sp. hordei Marchal) was identified to be Streptomyces species. The cultural filtrate of the Streptomyces sp. A 171 showed antifungal effect against the tested six soilbrone plant pathogenic fungi. Distinct antifungal activity was showed in tryptic soy broth medium at pH 6.5 and 30℃ during 72 hrs culture. The antifungal activity was stable in the pH range of 4 to 12 and high temperature(121℃).
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Bacillus subtilis B23 균주가 생산하는 항진균성물질의 분리 및 작용기작
이용세,이창호,송치현 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5
The antifungal substance was extracted from Bacillus subtilis B23 which is antagonistic against to some soil-borne plant pathogenic fungi. When the substance was extracted with organic solvents the activity was the highest in butanol. However, inpurity was lower and concentration steps were easier in ethyl acetate than other solvents. The column chromatography was carried out after absorption of the organic extract with XAD-2. The antifungal substance was eluted in 80-100% methanol and the active fractions were pooled. The pooled fractions were partially purified to obtain active antifungal substance by XAD-2 column chromatography. The biological activity of the antifungal substance was tested using TLC plate and the antifungal mechanism was studied.
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Bacillus subtilis B23 균주의 배양적 특성 및 항진균활성
이용세,이창호,송치현 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5
The growth rate and the antifungal activity of culture filtrate of the antagonistic microorganism Bacillus subtilis B23 was increased in tryptic soy broth (TSB) and yeast extract-malt extract broth (YMB). The cell growth and the antifungal activity of culture filtrate in TSB medium were optimized at 30 ℃ and pH 7.0. The cell growth of B23 strain reached to maximum at 21 hr after inoculation, wherease the antifungal activity of culture filtrate was shown to have the highest level at stationary phase (60 hr) after inoculation and was decreased a little after 72 hr incubation. The culture filtrate of B23 inhibited spore germination of Fusarium oxysporum.
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이용세,최장원,김상달,백형석 한국생명과학회 1999 생명과학회지 Vol.9 No.1
To isolate of antagonistic bacteria to Phytophthora capsici, which cause Phytophthora blight in red pepper, 237 isolates of Pseudomonas spp. and 260 isolates of Bacillus spp. were screened in selective media from rhizosphere soils of red pepper at Kyongsan, Kyongju, Yongchon and Euisung in Kyongbuk. Among total 497 isolates, 8 isolates of Pseudomonas spp and 4 isolates of Bacillus spp. inhibited the mycelial growth of Phytophthora capsici above 50$\%$ . These antagonistic bacteria showed more inhibitory effect on TSA (tryptic soy agar) than V-8 juice agar. Four isolates, P0704, P1201, B1101 and B1901, showing the most prominent antagonistic activity were selected and identified as P. cepacia (P0704, P1201), B. polymyxa (B1101) and B. subtilis (B1901), respectively. Cell free filtrates of these isolates were shown to inhibit zoosporangia germination and mycelial growth of p. capsici indicating that these isolates turned out to be bacteria producing antifungal substances. As a result of antagonistic test to Phytophthora blight in green house p. cepacia (P0704) showed the highest antagonistic effect with 46.7$\%$ and the rest of them were in the range of 13.4$\%$ to 26.7$\%$ .
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