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이용세,황병국 한국균학회 1982 韓國菌學會誌 Vol.10 No.4
Immature apple fruits of cultivars Fuji and Miller were completely resistant to Macrophoma sp. until 10 July. When inoculated on 31 July, apples became susceptible to this fungus, irrespective of cultivar. This fungus grew better on Fuji than Miller. Lesion enlargement on apple at 25℃ was remarkably pronounced relative to 20℃. In both cultivars, the amount of total soluble carbohydrates or reducing sugars in apple fruit flesh was gradually increased as apples became mature. The healthy apples of cultivar Fuji had a higher amount of carbohydrates than those of Miller. The infected apples contained more soluble carbohydrates in comparison to the healthy ones. In healthy apples, there were no consistent differences between Fuji and Miller in amount of total soluble amino acids. With increasing age of apples, the amount of soluble amino acids declined in the fruit flesh of both cultivars. Levels of carbohydrates and amino acids in apple fruits were discussed in association with the immature fruit resistance to Macrophoma sp.
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고추잎 열수추출물로부터 보체계 활성화 다당의 분리 및 특성
이용세,나경수,정상철,서형주,박희성,백형석,최장원 한국생명과학회 2002 생명과학회지 Vol.12 No.1
It was observed that the hot water extract of the leaves of Capsicum annuum L., a Korean edible plant, had a potent anti-complementary activity. Crude polysaccharide fraction(CAL-0) was obtained by methanol reflux, ethanol precipitation, dialysis and lyophilization. CAL-0 contained 51.8% of total sugar, 8.2% of uronic acid and 16.8% of protein, and consisted of mainly arabinose, galactose and glucose as neutral sugars and galacturonic acid as uronic acid. The anti-complementary activity of CAL-0 decreased greatly by periodate oxidation, but was not changed by pronase treatment. Also, the anti-complementary activity of CAL-0 was reduced partially in the absence of the $Ca^{2+}$ ion. The crude polysaccharide CAL-0 was found to activate the C3 component both in the presence and in the absence of $Ca^{2+}$ through the crossed-immunoelectrophoresis suggesting that those involved in both classical and alternative complement pathway CAL-0 was further separated to an unabsorbed fraction(CAL-1) and six absorbed fractions(CAL-2longrightarrowCAL-7) on DEAE Sepharose CL-6B ion exchange column. Among them four major fractions in activity and yield were obtained, and consisted mainly of arabinose, galactose and glucose with various molar ratios. The major fraction, CAL-2, was purified to give a high molecular fraction(CAL-2-I) and a low molecular fraction(CAL-2-II) on Sepharose CL-6B column. The anti-complementary activity of CAL-2-I, a molecular weight of about 61,000, was higher than it of CAL-2-II.-II.
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이용세,최장원,김상달,백형석 한국생명과학회 1999 생명과학회지 Vol.9 No.1
To isolate of antagonistic bacteria to Phytophthora capsici, which cause Phytophthora blight in red pepper, 237 isolates of Pseudomonas spp. and 260 isolates of Bacillus spp. were screened in selective media from rhizosphere soils of red pepper at Kyongsan, Kyongju, Yongchon and Euisung in Kyongbuk. Among total 497 isolates, 8 isolates of Pseudomonas spp and 4 isolates of Bacillus spp. inhibited the mycelial growth of Phytophthora capsici above 50$\%$ . These antagonistic bacteria showed more inhibitory effect on TSA (tryptic soy agar) than V-8 juice agar. Four isolates, P0704, P1201, B1101 and B1901, showing the most prominent antagonistic activity were selected and identified as P. cepacia (P0704, P1201), B. polymyxa (B1101) and B. subtilis (B1901), respectively. Cell free filtrates of these isolates were shown to inhibit zoosporangia germination and mycelial growth of p. capsici indicating that these isolates turned out to be bacteria producing antifungal substances. As a result of antagonistic test to Phytophthora blight in green house p. cepacia (P0704) showed the highest antagonistic effect with 46.7$\%$ and the rest of them were in the range of 13.4$\%$ to 26.7$\%$ .
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Streptomyces sp. A252의 배양적 특성 및 항진균활성
이용세,최장원,라경수,백형석 한국생명과학회 1999 생명과학회지 Vol.9 No.1
The growth rate of the A252 strain was increased in tryptic soy broth (TSB) and malt extract-yeast extract medium (ISP-2), but the antifungal activity of culture filtrate was efficient in the media of TSB and nutrient broth. The mycelial growth and the antifungal activity of culture filtrate in TSB medium were optimized at $25^{\circ}C$ and pH 6.5. The growth in 2$\%$TSB concentration was more effective than 1$\%$, but there was no difference of the antifungal activity by the TSB concentrations. The mycelial growth of A252 strain reached to maximum at 72 hr after inoculation, whereas the antifungal activity of culture filtrate was shown to have the highest level at idiophase (60 hr) after inoculation and was decreased a little after 96 hr incubation. The antifungal activity was stable in the pH range of 4 to 11 and evenly at $121^{\circ}C$. The A252 strain was characterized as Streptomyces species by the physiological properties and examination of sporophore me morphology.
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Bacillus subtilis B23 균주의 배양적 특성 및 항진균활성
이용세,이창호,송치현 대구대학교 과학기술연구소 1999 科學技術硏究 Vol.5 No.5
The growth rate and the antifungal activity of culture filtrate of the antagonistic microorganism Bacillus subtilis B23 was increased in tryptic soy broth (TSB) and yeast extract-malt extract broth (YMB). The cell growth and the antifungal activity of culture filtrate in TSB medium were optimized at 30 ℃ and pH 7.0. The cell growth of B23 strain reached to maximum at 21 hr after inoculation, wherease the antifungal activity of culture filtrate was shown to have the highest level at stationary phase (60 hr) after inoculation and was decreased a little after 72 hr incubation. The culture filtrate of B23 inhibited spore germination of Fusarium oxysporum.
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