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이영훈 ( Yeong Hoon Lee ),임승택 ( Seung Taek Lim ),윤영남 ( Young Nam Yoon ),전명기 ( Myeong Gi Jeon ),윤홍태 ( Hong Tae Yun ),고종민 ( Jong Min Ko ),이수헌 ( Su Heon Lee ),이기운 ( Key Woon Lee ),백인열 ( In Youl Baek ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.29 No.1
국내 콩에서는 SMV, SbDV, AMV, CMV, CPMV, SYMMV, SYCMV와 PSV가 보고되었다. 과거 이들 바이러스 중에서 SMV가 심각한 피해를 입히고 있으며, 90%이상 우점하는 것으로 보고되었다. 하지만, 최근 SMV의 발생률은 50%정도로 낮아졌으며, SYMMV, SYCMV와 PSV 등에 의한 여러 가지 바이러스 병해가 피해를 주고 있는 것으로 확인되었다. 최근 기후 및 재배환경의 변화와 국제 농산물 교역으로 인해 병 발생 양상이 급속하게 변하고 있음을 나타내는 단적인 예인 것이다. 또한, 새로운 매개층과 전염원의 발생으로 신종 또는 미보고 바이러스 발생이 늘어나고 있지만, 콩과 같은 주요 작물의 병해 발생 상황 및 피해 양상 구명에 대한 연구는 미흡한 실정이다. 이러한 이유로 국내 콩에서 발생하는 바이러스 병해의 확인을 위해 8도19지역에서 193점의 시료를 채집하였다. 채집된 시료들은 SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV와 CPMV 14종에 대한 정밀 진단을 위하여 각각의 종특이 프라이머를 이용하여 RT-PCR 진단이 수행되었다. 그 결과 채집된 시료의 86%가 바이러스에 감염된 것으로 확인 되었으며, SMV 141, SYMMV 14, PSV 8과 SYCMV 5점이 확인되었다. 대구와 나주에서 채집된 2점의 시료들에서는 SYMMV와 SYCMV가 복합감염 되어있었다. 나머지 미동정 시료에 대한 정밀 분석과 지속적인 발생상황 조사가 이루어 져야 할 것이다. It had been reported that soybean viral diseases are Soybean mosaic virus (SMV), Soybean dwarf virus (SbDV), Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV), Cowpea mosaic virus (CPMV), Soybeun yellow rnottle mosaic virus (SYMMV), Soybean yellow common mosaic virus (SYCMV) and Peanut stunt virus (PSV) in Korea. Among these viral diseases, SMV caused severe damage to soybean in Korea, Although SMV occurred more than 90% in the past, recently several viruses such as SYMMV, SYCMV, SbDV and PSV have been reported in Korea, It means that the incidence of viral diseases are changing in soybean. To identify the viruses infecting soybean in Korea, the 193 samples with viral symptoms were collected in 19 areas of 8 provinces. And then the RT-PCR assay was conducted to detect 14 different viruses such as SMV, SYMMV, SYCMV, SbDV, PSV, BCMV, AMV, PEMV, CMV, CCMV, TSV, BBWV2, BYMV and CPMV. The results indicated that about 86% of samples were identified as Virus-infected, Among 193 soybean samples, 141 SMV, 8 PSV, 14 SYMMV, 5 SYCMV were detected. Two samples were coinfected with SYMMV and SYCMV. The rest of them were likely to express the mosaic similar to virus-induced symptoms. Therefore, the identifications of the unknown samples have been performed by the dererminatlon of the nucleotide sequences of the genomic RNAs.
이영훈(Lee Young-Hoon),김종기(Kim Jong-Ki),류상환(Ryu Sang-Hwan),이영형(Lee Yeong-Hyeong) 한국철도학회 2001 한국철도학회 학술발표대회논문집 Vol.- No.-
본 논문은 고정폐색구간에서 차량운전 특성과 속도패턴이 다양한 다종의 열차들이 동일선로에서 복합되어 고밀도 운행을 위한 방법으로, 신호현시에 대한 제반정보를 차상으로 전송받아 기관실 신호표시반에 신호를 현시하는 차상신호방식(Cab Signalling System)중에서, 선행열차의 위치, 선로조건 등 지상의 상태정보를 후속열차가 수신하여 열차 자신의 제동특성 등 차량정보와 비교 분석하여 제동거리를 계산하는 distance to go 방식으로 열차운전속도를 제어하는 ATP (Automatic Train Protection) 시스템을 제안하였다. 폐색구간별 고정된 신호속도단계가 부여되는 ATC(Automatic Train Control) 시스템에서는 운행패턴이 같은 단일차종의 열차만 운행하는 구간에서는 일정속도에 의한 폐색구간 분할로 구간내 distance to go 신호체계가 이루어지지만, 차종과 속도패턴이 서로 상이한 복합열차들의 운행구간에서는 안전거리 확보를 위하여 저속열차의 제동거리를 기준으로 폐색구간 분할 및 속도결정이 이루어지므로 ATP 방식보다 운행효율이 다소 떨어짐을 알 수 있었다. 이를 차량특성과 속도패턴이 다른 3개의 열차를 모델로 하여 열차별 감속도에 의한 제동특성을 ATC와 ATP 선호제어체계에 접목시켜 시뮬레이션을 하였다.
콩에 발생하는 세균병의 종자 오염 및 불마름병과 들불병 저항성 평가
이영훈 ( Yeong Hoon Lee ),전명기 ( Myeong Gi Jeon ),최만수 ( Man Soo Choi ),강범규 ( Beom Kyu Kang ),김현영 ( Hyun Young Kim ),윤홍태 ( Hong Tae Yun ),백인열 ( In Youl Baek ),윤영남 ( Young Nam Yoon ) 한국콩연구회 2013 韓國콩硏究會誌 Vol.30 No.1
It has been reported that bacterial diseasees of domestic soybean are bacterial pustule (Xanthomonas axonopodis pv. glycines), wildfire ( Pseudomonas syringae pv. tabaci), bacterial blight (Pseydomonas savastanoi pv. glcines) and bacterial brown spot (Pseudomonas syringae pv. syringae) in Korea. Bacterial pustule had been the most issue in soybean diseases. In recently wildfire also occurrence in soybean and Bacterial blight and bacterial brown spot have been reported to less incidence in Korea. Each bacterial diseases were seed borne and then they caused diseases as orumary casual agent. in this study, direct OCR assay was applied to detect casual agent of bacterial diseases and survey on seed contamination on 44 soybean cultivars and 14 genctic resources in Korea, The pathogens were detected frin the seed samples incubated for 12h with 160rpm shaking. The result of surbey on seed contamination showed that bacterial pustule, wildfire and bacterial blight were detected from some cultivars and genetic resources. We also conducted resistance screening in other to select resistance cultivars of Baterial pustule and Wildfire. we inoculated on 128 cultivars with Baterial pustule and Wildfire respectively. These cultivars were classified through pathoggenecity from 1 to 9. In addition, 35 and 50 resistant cultivars were confirmed against Baterial pustule and Wildfire respectively
이태훈,남일현,문기혁,이영현,안길영,이영훈,Lee, Tae-Hoon,Nam, Il-Hyun,Moon, Gi-Hyuk,Lee, Yeong-Hyeon,Ahn, Gil-Yeong,Lee, Young-Hoon 대한족부족관절학회 2015 대한족부족관절학회지 Vol.19 No.1
Accessory navicular is a congenital anomaly appearing in the secondary ossification center on the tuberosity of the navicular that may cause flatfoot. Bipartite medial cuneiform is another rare congenital anomaly occurring as two primary ossification centers in the medial cuneiform. The authors report a rare case of symptomatic bilateral accessory navicular with bipartite medial cuneiform and flatfoot deformity in a 19-year-old man with a review of the literature.
이영현,안길영,남일현,이태훈,이용식,김대근,이영훈,Lee, Yeong Hyeon,Ahn, Gil Yeong,Nam, Il Hyun,Lee, Tae Hun,Lee, Yong Sik,Kim, Dae Geun,Lee, Young Hoon 대한족부족관절학회 2016 대한족부족관절학회지 Vol.20 No.4
Purpose: To evaluate the effect of shortening scarf osteotomy on pain relief and range of motion (ROM) of the first metatarsophalangeal joint in hallux rigidus patients. Materials and Methods: Twenty-three cases of 19 patients who had been treated with shortening scarf osteotomy for the hallux rigidus between January 2007 and December 2013 were reviewed. The mean follow-up period was 21.4 months, and the mean age was 59.2 years. The first metatarsal bone was shortened until the ROM of the first metatarsophalangeal joint was greater than $80^{\circ}$ or $40^{\circ}$ of dorsiflexion. The length shortened by scarf osteotomy was measured. The authors also measured and compared the joint interval difference of the standing foot using an anteroposterior radiography. Moreover, the difference of ROM of the first metatarsophalangeal joint between the preoperative and final follow-up periods was also compared. The clinical results were evaluated and compared using the American Orthopaedic Foot and Ankle Society (AOFAS) scoring system and visual analogue scale (VAS) score. Results: The mean shortening length was about 6.5 mm (range, 4~9 mm). The joint space has been increased to 1.8 mm, and the ROM of the first metatarsophalangeal joint has also been increased to $18.4^{\circ}$ after the operation. In three cases, the postoperative ROM has been decreased to less $10^{\circ}$. The AOFAS score has been improved from 41.7 (range, 32~55) to 86.2 (range, 65~95), and the VAS score was also decreased from 3.7 (range, 3~5) to 1.3 (range, 0~3). Two cases have shown no decrease in pain even after the operation. Conclusion: Shortening scarf osteotomy was found to decrease joint pain by decompressing the pressure of the first metatarsophalangeal joint. This osteotomy also helped improve the ROM of the first metatarsophalangeal joint. Shortening scarf osteotomy can be considered one of the effective methods for joint preservation.
콩 불마름병 저항성 및 감수성 품종을 이용한 rxp 유전자 근접 분자표지 개발
양기웅(Kiwoung Yang),이영훈(Yeong Hoon Lee),고종민(Jong Min Ko),전명기(Myeong Gi Jeon),이병원(Byong Won Lee),김현태(Hyun Tae Kim),윤홍태(Hong Tae Yun),정찬식(Chan Sik Jung),백인열(In Youl Baek) 한국육종학회 2011 한국육종학회지 Vol.43 No.4
본 연구는 최근 콩 재배에서 심각한 병으로 대두된 콩 불마름병에 대한 저항성 유전자인 rxp 근접분자표지를 개발하고 자수행하였다. 콩 불마름병은 국내에서 전국적으로 발생하는 심각한 세균병으로 이에 관련하여 세균병 접종을 이용한 저 항성 품종과 감수성 품종에 대한 연구가 많이 진행되고 있지 만 정확한 유전자의 염기서열이 밝혀져 있지 않고 있다. 본 연구는 콩 불마름병에 저항성 품종 8개체와 감수성 품종 8개체를 이용하여 rxp 유전자 근접분자표지를 확인하기 위하여 수행하였다. 콩 불마름병 저항성 유전자로 알려진 rxp 유전자는 chromosome 17의 Satt486과 Satt372 사이에 있다고 알려져 있으며, 최근 연구결과로 chromosome 17의 7.27-7.30 Mbp 사이에 있다. 연구진은 chromosome 17의 6.6-7.3 Mbp 사이 에 random으로 분자표지를 제작하여 저항성과 감수성 품종 에서 다형성을 알아보았다. 실험결과로 콩 불마름병관련 근접 분자표지 3점을 개발하였고, Rxp17-700 분자표지는 흥미로운 rxp 근접분자표지임을 확인하였다. 이러한 콩 불마름병 저항성관련 근접분자표지는 앞으로 저항성 품종을 선발하는데 도움이 될 것이다. Bacterial pustule (BP) is a leaf disease of soybean that is most common in Korea. Inoculation of 8ra, pathogen strain, to resistant and susceptible cultivars for finding the BP resistance gene (rxp) was much tried but the sequence of the exact gene is not found. This research performed in order to confirm the rxp gene near molecular marker by using the resistant and susceptible cultivars. Soybean BP resistance gene which related to region of near molecular marker could select the resistant cultivar. For the near molecular marker of rxp, reference genomics data available at sequenced Phytozome was used for designing molecular markers. The rxp was mapped between Satt372 and Satt486 on chromosome 17. According to previous study, rxp released in find mapping 7.2 Mbp to 7.3 Mbp on chromosome 17. In this study, we developed 3 random markers near from 6.6 Mbp to 7.3 Mbp on chromosome 17 identified to increase the genetic resolution of the rxp gene region using resistant and susceptible cultivars. Particularly, Rxp17-700 marker was mostly coincided resistance and susceptible genotype to rxp. This result suggests that Rxp17-700 marker will be more tightly linked to rxp gene.