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조지은(Ji Eun Jo),이아영(A Yeong Lee),김효선(Hyo Seon Kim),문병철(Byeong Cheol Moon),지윤의(Yunui Ji),천진미(Jin Mi Chun),김호경(Ho Kyoung Kim) 한국식품과학회 2013 한국식품과학회지 Vol.45 No.3
A quantitative method using ultra performance liquid chromatography with a photodiode array detector (UPLCPDA) was established for the analysis of 2 major plant metabolites: β-asarone and α-asarone from Acorus gramineus, A. tatarinowii, A. calamus and Anemone altaica, and their contents are compared with other herbs of Acorus species. The method was validated according to the International Conference on harmonization (ICH) guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The average content of β-asarone in Acorus gramineus was significantly higher than that in others, with the second highest concentration observed in A. tatarinowii, and only a trace amounts found in A. calamus and Anemone altaica. In contrast, the average content of α-asarone in A. calamus was the highest, followed by that in Acorus gramineus and A. tatarinowii. principle component analysis (PCA) confirmed that β-asarone and α-asarone content differed among the species. These results suggest that this UPLC-PDA method can be considered as good quality control criteria for Acorus gramineus.
이아영(Lee A-Yeong),김희창(Kim hee-Chang),정제창(Jeong Je-Chang) 한국방송·미디어공학회 2009 한국방송공학회 학술발표대회 논문집 Vol.2009 No.11
영상 표시장치의 화소수가 다양화됨에 따라, 영상 보간법은 더욱 중요한 역할을 하게 되었다. 3차 콘볼루션 보간법(Cubic Convolution Interpolation)은 간단하지만, 적용하는데 제한이 없고, 좋은 성능을 보이기 때문에 널리 쓰이고 있다. 이 논문은 3차 콘볼루션 보간법을 이용한 적응적 방법을 제안한다. 예측하려는 픽셀의 이웃 화소 값의 차이를 고려해서, 3차 콘볼루션 커널에 있는 ‘a’값을 적응적으로 선택한다.
KHP 기준에 의한 보험용 단미엑스산제의 지표성분함량 확인연구
박상준 ( Sang Jun Park ),김소형 ( So Hyung Kim ),김경석 ( Kyeong Seok Kim ),김효선 ( Hyo Seon Kim ),이아영 ( A Yeong Lee ),김호경 ( Ho Kyoung Kim ),김윤경 ( Yun Kyung Kim ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.3
Objectives : Since single extract powders was released at 1987, the insurance fee has not been changed, but the price of raw material has been increasing. According to this, Pharmaceutical company couldn`t invest on quality of the product, so, the quality of single extracts went down and lost the consumer`s trust. We checked the contents of marker ingredients in single extract thereby to recover the reliability of insurance-covered herbal preparations. Methods : we bought total twelve products of eight different single extracts of two main pharmaceutical companies among total 65 kinds of single extract powder stipulated in KHP (The Korean Herbal Pharmacopoeia) monograph Ⅲ at Jan. 2013. Assays of selected single extract powders are performed by KHP regulation. And we surveyed price fluctuation of relevant herb raw materials from 2005 to 2012. Results : Among twelve products, eight single extract powders were suitable by the KHP regulation. But four products didn`t reach the content amount of KHP. Marker contents in the single extracts product of Pueraria Root, Licorice, Peony root and Scutellaria Root of A company were 70%, 1%, 23.7% and 75.1%, respectively. Conclusions : We can acertain whether there`s a quality problem in the insurance-covered single extract powders. But, A company is no longer producing these improper single extract powders. As a medicine, single extract powders needs to be strictly quality controlled by the company, and regularly monitored by the KFDA.
이아영 ( A Yeong Lee ),김효선 ( Hyo Seon Kim ),최고야 ( Go Ya Choi ),천진미 ( Jin Mi Chun ),문병철 ( Byeong Cheol Moon ),김호경 ( Ho Kyoung Kim ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6
Objectives: The Illicii Veri Fructus was not only traditional medicine but also food in Asia. The aim of this study was selection of optimum solvent in the fruit of Illicii Veri Fructus because an appropriate solvent affect a medicinal effect. Methods : Illicii Veri Fructus was carried out ultrasonic-assisted extraction as various solvents. Two main compounds, p-anisaldehyde and anethole, were successfully analyzed by high performance liquid chromatography-photodiode array detector (HPLC-PDA) and carried out method validation according to ICH guideline. The optimum solvent selected by comparing with yields of two main ingredients. Results : The p-anisaldehyde and anethole were detected at approximately 8.0 min and 19.8 min, respectively. It was all below 5.0% that RSD of retention time and peak area for two main peaks. Calibration curves of two compounds were good linearity as R2 >0.9999. All of the precisions and accuracy were good intra-day and inter-day as below 5.0% RSD. Limited of detection (LOD) of p-anisaldehyde and anethole were analyzed as 0.134 μg/mL and 4.286 μg/mL, respectively. Limited of quantification (LOQ) of two compounds were 0.407 μg/mL and 12.989 μg/mL, respectively. As a result of this study, p-anisladehyde was detected as 0.209 ~ 0.467%, however anethole was not detected in the distilled water. Conclusions : Anethole was main component as 5.329 ~ 6.815% except for water extraction. Methanol extraction among various solvents was detected the highest contents of p-anisaldehyde and anethole as 0.467(±0.008)% and 6.815(±0.220)%, respectively.
이혜원(Lee Hye-Won),박소영(Park So-Young),추병길(Choo Byung-Kil),채성욱(Chae Sung-Wook),이아영(Lee A-Yeong),김호경(Kim Ho-Kyoung) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.3
Objective : Polygonum multiflorum Thunberg (Polygonaceae) has been traditionally used as a tonic and the purgative in China and Korea. The genus Polygonum is a source of a wide range of phenolic compound, flavanoids, anthraquinones, stilbenes and tannins. In this study, three anthraquinones were isolated and quantitative determination of anthraquinones from P multiflorum has been developed for quality standardization. Methods : Three anthraquinone derivatives were isolated from a methanol extract of the radix of P. multiflorum by the chromatographic separation. Their structures were identified as emodin, physcion and ω-hydroxyemodin on the basis of spectral data (MS, 1H-NMR, 13C-NMR) and chemical analysis. HPLC analysis was performed to determine the contents of emodin, physcion, chrysophanol, rhein and ω-hydroxyemodin in P multiflorum from different specimens were collected from twenty Korean markets. Results : According to the results, the contents of emodin, physcion, chrysophanol, rhein and ω-hydroxyemodin were 0.145%, 0.434%, 0.016%, 0.026%, 0.030% by HPLC, respectively. Conclusions : In these results, we have determined the contents of emodin, physcion, chrysophanol, rhein and ω-hydroxyemodin in P. multiflorum, respectively. We hope that this study will contribute to the standardization and quality control of herbal medicine.
전원경(Jeon Won Kyung),김호경(Kim Ho Kyoung),이주현(Lee Ju Hyun),고병섭(Ko Byoung Seob),이아영(Lee A Yeong),이경구(Lee Kyung Goo),신현규(Shin Hyeun Kyoo) 한국한의학연구원 2007 한국한의학연구원논문집 Vol.13 No.2
The present study examined inhibitory effects of 20 efficient experience prescriptions on platelet aggregation induced by collagen in human whole blood using the impedance method of aggregometry. Among them, a hot water extract of KIOM 2003-080 was selected to be the most effective candidate. In an in vivo study using a mouse acute thrombosis model, the anti-thrombotic effects of the KIOM2003-080 crude extract were also observed. In addition, we accessed bio-marker of platelet activation using thromboxane B2 by ELISA assay. A significantly decrease in thromboxane B2 production was seen in the presence of KIOM2003-080. Consequently, the results from this experiment provide pharmacological evidence for the traditional use of KIOM2003-080 prescription, suggesting that its hot water extracts could be used to prevent platelet aggregation and thrombosis disease.
이주현,전원경,고병섭,천진미,이아영,김호경,Lee, Ju-Hyun,Jeon, Won-Kyung,Go, Byoung-Seob,Chun, Jin-Mi,Lee, A-Yeong,Kim, Ho-Gyoung 한국한의학연구원 2006 한국한의학연구원논문집 Vol.12 No.1
한약재는 생산, 가공, 유통과정에서 적절한 보관. 관리가 이루어지지 않으면 미생물 증식 가능성이 크고 이로 인한 부패나 변질이 우려되며 한약 품질 저하를 가져올 수 있다. 한약재 미생물허용한도에 대한 각 나라별 규격기준을 보면 유렵약국, 도일약국에서는 호기성균의 미생물오염한도(microbial contamination limit)를 10 CFU/g 이하로 규격화하고 있다. 또한 미국약국 NF(US phamacopeia & National Formulary)에서는 생약의 미생물 한도치를 유럽약국과 같은 정도의 규격설정을 하고 있다. 특히 일본의 경우 일본약국방(日本藥局方) 및 국방외생약규격집(局方外生藥規格集)에 의해서 한약의 품질을 유지하고 있고, 미생물학적 품질관리 실태조사가 체계적으로 이루어지고 있다. 본 연구에서는 3개 지역에서 각각 1곳의 약업사를 정하여 대상품목 총 30종을 선정하였다. 실험의 재료는 문헌과 자료조사에 의해 미생물에 의한 오염이 용이한 품목 18종, 육진약(六陳藥)에 속하는 6종, 그리고 육미지황환을 구성하는 6종을 구입하여 대한약전 8개정의 미생물한도 시험법에 따라 시험하였다. 한약재에 대한 미생물 오염 실태조사를 통하여 미생물 허용한도를 설정하는데 기초 자료로 제시하고자 한다. 한약재 미생물 오염 실태조사 결과 WHO 미생물한도 기준을 적용하였을 때, 전체적으로 진균의 오염이 높은 경향을 보였고 향후 한약재에 대한 미생물오염도 모니터링을 지속적으로 하여야 할 것으로 사료된다. Objectives : This study has attempted to establish an organized system for the microbiological quality of raw materials which are important factors in preparing the standard for microbial limits. Methods : This study has first set up microbial contamination limit test. total aerobic microbial count and total fungi count, int accordance with testing method of the Korea Pharmacopeia 8th edition in order to establish an inspection standard for microbial contamination. Results : The microbial contamination of 18 items that are highly prone to contamination by three regions(Seoul, Daejeon, Gyeongsangbuk-do), As a result, Morus alba Linne and Rehmannia glutimosa Liboschitz var. purpurea Makino showed as high contaminated by WHO's Microbial Contamination Limit standard. In case of Yukjin medicine in the Theory of Herb Medicinal Properties, total bacterial contamination rate showed as 17.7%, total fungal contamination rate showed as 41.2% and total aerobic mircobial count and total fungi count on Ephedra sinica Stapf. Pinellia ternate Breitenbach, Evodia officinalis Dode showed as high measured. The microbial conatmination rate materials which make up Yukmijihwanghwan were mostly high therefore the total aerobic microbial count was measured as high in case of Yukmijihwanghwan, the characteristics about microbial contamination strain is to be researched. Conclusions : By combining the basic data and experimental results related to microbial contamination of herb medicine, the most ideal storage standards for herb medicine has been attempted to be presented.