RISS 학술연구정보서비스

검색
다국어 입력

http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.

변환된 중국어를 복사하여 사용하시면 됩니다.

예시)
  • 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
  • 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
닫기
    인기검색어 순위 펼치기

    RISS 인기검색어

      검색결과 좁혀 보기

      선택해제
      • 좁혀본 항목 보기순서

        • 원문유무
        • 원문제공처
          펼치기
        • 등재정보
          펼치기
        • 학술지명
          펼치기
        • 주제분류
          펼치기
        • 발행연도
          펼치기
        • 작성언어
        • 저자
          펼치기

      오늘 본 자료

      • 오늘 본 자료가 없습니다.
      더보기
      • 무료
      • 기관 내 무료
      • 유료
      • 항 히스타민제의 H₁ 수용체와 무스카린 수용체에 대한 상대적 역가

        이신웅,박영주,이정수 영남대학교 약품개발연구소 1994 영남대학교 약품개발연구소 연구업적집 Vol.4 No.-

        The muscarinic antagonist l-[benzilic-4, 4'-³H]quinuclidinyl benzilate([³H]QNB) bound to a single class of muscarinic receptor with high affinity in guinea pig ileal membranes. The K_(D) and B_(max) values for [³H]QNB calculated from analysis of saturation isotherms were 54 pM and 156 fmol/mg, respectively. H₁-blockers inhibited [³H]QNB binding to ileal membranes with K_(i) values ranged from 0.008 μM to 1.6 μM. The pseudo-Hill coefficients of H₁-blockers for inhibition of [³H]QNB binding to the ileal membranes were close to unit. The K_(i) values for H₁-blockers were similar to the K_(M) values calculated by Schild plot of functional data obtained from inhibition of the carbachol-induced contraction in guinea-pig ileum, suggesting that binding of H₁-blockers vs [³H]QNB in ileal membranes represents an interaction with a receptor of physiological relevance. The K_(H) values of H₁-blockers for H₁-receptor estimated from inhibition of the histamine-induced contraction were the range of 0.15 nM to 56.5 nM. The K_(M)/K_(H) ratio of H₁-blockers varied over a wide range of 3 to 2300. Thus, the antihistaminic potencies of H₁-blockers do not correlate with their antimuscarinic potencies, which suggest that antihistamines have different antimuscarinic potencies in therapeutic blood levels causing similar antiallergic effect. Among 13 traditional antihistaminics examined in this study, drug having the highest and the lowest K_(M)/K_(H) ratio is triprolidine and diphenidol, respectively. The present results demonstrate that the antimuscarinic property of antihistamines is not necessary for their antiallergic effect, and data on the affinity of antihistamines for muscarinic and H₁-receptors can be an important parameter in the selection and evaluation of these drugs.

      • Oxomemazine의 Muscarinic Receptor Subtypes에 대한 결합성질

        이신웅,김정구 大韓藥理學會 1994 대한약리학잡지 Vol.30 No.1

        '스콜라' 이용 시 소속기관이 구독 중이 아닌 경우, 오후 4시부터 익일 오전 9시까지 원문보기가 가능합니다.

        Oxomemazine이 muscarinic receptor subtypes에 대하여 선택성을 가지는지에 관한 지견을 얻고자, 대뇌, 심실 및 회장 muscarinic receptor에 대한 oxomemazine의 결합성질을 조사, 비교하였다. <TEX>$[^3H]QNB$</TEX> 포화결합실험 결과 세 조직의 muscarinic receptor는 <TEX>$[^3H]QNB$</TEX>에 대해서는 affinity가 약 60pM인 단일 receptor인 것으로 추정되었다. 대뇌에서 pirenzepine과 oxomemazine의 <TEX>$[^3H]QNB$</TEX> 결합억제에 대한 Hill coefficient는 각각 0.67 및 0.8로서 대뇌에는 이들 약물에 대하여 affinity가 서로 다른 두 종류의 muscarinic receptor subtypes가 존재하는 것으로 나타났으며, pirenzepine에 대한 high <TEX>$affinity(M_1)$</TEX>와 <TEX>$low affinity(M_2)$</TEX> receptor 및 oxomemazine에 대한 high <TEX>$affinity(O_H)$</TEX>와 <TEX>$low\;affinity (O_L)$</TEX> receptor의 분포비는 약 60:40 및 40:60이었고, <TEX>$M_1$</TEX>과 <TEX>$M_2$</TEX> receptor에 대한 pirenzepine의 <TEX>$K_i$</TEX>치는 16nM 및 431 nM, <TEX>$O_H$</TEX>와 <TEX>$O_L$</TEX>, receptor에 대한 oxomemazine 의 <TEX>$K_i$</TEX>치는 80nM 및 1350nM이었다. 그러나 심실과 회장에서 이들 약물의 <TEX>$[^3H]QNB$</TEX> 결합억제에 대한 Hill coefficient는 1에 가까웠다. 심실과 회장 muscarinic receptor에 대한 pirenzepine의 <TEX>$K_i$</TEX>치는 850nM 및 250nM, oxomemazine의 <TEX>$K_i$</TEX>치는 1460nM 및 670nM로서 대피에서 이들 약물의 low affinity receptor에 대한 <TEX>$K_i$</TEX>치에 가까웠다. 즉, muscarinic receptor에 대한 affinity면에서 oxomemazine은 pirenzepine과 같이 대뇌에서 가장 높았으며, 회장에 대해서는 중등도였고, 심실에서 가장 낮았다. 이로 보아 oxomemazine은 <TEX>$M_1\;receptor$</TEX>에 선택성이 있는 것으로 추정된다. The binding properties of oxomemazine to muscarinic receptors using the ability of oxomemazine to inhibit <TEX>$[^3H]QNB$</TEX> binding in membrane fractions of rat cerebrum and guinea pig ventricle and ileum were investigated. <TEX>$[^3H]QNB$</TEX> bound to a single class of muscarinic receptors with a dissociation constant of approximately 60 pM in three tissue preparations. Pirenzepine and oxomemazine inhibited <TEX>$[^3H]QNB$</TEX> binding in cerebrum with a Hill coefficient lower than unity, and the inhibition data were best described by a two-site model. The relative densities of the high <TEX>$(M_1)\;and\;low\;(M_2)$</TEX> affinity sites for pirenzepine were 60 and 40%, with corresponding Ki values of 16 and 431 nM, and those <TEX>$(O_H\;and\;O_L)$</TEX> for oxomemazine 40 and 60%, with corresponding Ki values of 80 and 1350 nM. However, the inhibition data of both drugs vs <TEX>$[^3H]QNB$</TEX> in ventricle and ileum appeared to obey the law of mass-action (Hill coefficient close to 1). The apparent Ki values of pirenzepine were 850 and 250 nM, and those of oxomemazine 1460 and 570 nM in ventricle and ileum, respectively. Thus, oxomemazine like pirenzepine has high affinity for cerebrum, moderate affinity for ileum and low affinity for ventricle. These results suggest that oxomemazine could recognize the muscarinic receptor subtypes with a high affinity for the <TEX>$M_1$</TEX> sites.

      • SCIESCOPUSKCI등재

        칼슘 길항제가 심장 ${\beta}$-Adrenergic Receptors에 미치는 영향

        이신웅,김정구 한국응용약물학회 1993 Biomolecules & Therapeutics(구 응용약물학회지) Vol.1 No.1

        It has been known that calcium antagonists also inhibit the radioligand binding to muscarinic and $\alpha$-adrenergic receptors and, in case of verapamil, these inhibitions may play a role in the effects of verapamil on the heart. In this study, the effects of nicardipine, nifedipine, nimodipine, diltiazem and verapamil on the binding of [$^3H$]dihydroalprenolol (DHA) to dog cardiac ${\beta}$-adrenergic receptors were examined. A single uniform [$^3H$]DHA binding site ($K_D/= 5nM\;and\;B_{max}=2600$ fmol/mg protein) was identified in dog cardiac sarcolemma. [$^3H$]DHA binding was not affected by the usual therapeutic concentrations of these calcium antagonists (nanomolar range) but in the "nonspecific"concentration ranges ($28-180{\mu}m$) these drugs inhibited [$^3H$]DHA binding to $\beta$-adrenergic receptors. Nicardipine, nifedipine, nimodipine and diltiazem competed for [$^3H$]DHA binding to ${\beta}$-adrenergic receptors with dissociation constants ($K_i$) of $28{\mu}m,\' 74{\mu}m, 39{\mu}m \;and \;35{\mu}m,$ respectively. Verapamil ($K_i=176.5 {\mu}m$) was less potent inhibitor than other drugs and this inhibition was noncompetitive; the maximal binding capacity ($B_{max}$) $300 {\mu}m$ verapamil without change in the apparent dissociation constant (4K_D$) for DHA. These results indicate that the inhibitory action of calcium antagonists at high concentrations on ${\beta}$-adrenergic receptors is not involved in the therapeutic effects of these drugs by the calcium channel blocking action.

      • KCI등재

        ESL students' beliefs about the utility of synchronous online discussion (SOD) in language learning and their participation in the SOD.

        이신웅 한국멀티미디어언어교육학회 2005 Multimedia Assisted Language Learning Vol.8 No.2

        The purpose of the study was to examine students' beliefs about the utility of synchronous online discussion (SOD) in language learning and their participation in the SOD and how they were related by using both quantitative and qualitative research methods. Thirty-four ESL students from diverse cultural backgrounds who were enrolled in an English Language Program (ELP) at a university in the U.S. participated in the study. The results showed that the students' beliefs were significantly related to their participation in the SOD, and could explain such participation considerably even after controlling their English proficiency. On the basis of the results, it is argued that teachers need to help students to understand more explicitly the purpose of participating in synchronous online discussions and how they are relevant to their language learning. Otherwise, the potential benefits of the use of SOD in language learning may not accrue to learners as expected.

      • Binding Profiles of Oxomemazine to the Muscarinic Receptor Subtypes

        이신웅,김정구,Lee, Shin-Woong,Kim, Jeung-Gu The Korean Society of Pharmacology 1994 대한약리학잡지 Vol.30 No.1

        Oxomemazine이 muscarinic receptor subtypes에 대하여 선택성을 가지는지에 관한 지견을 얻고자, 대뇌, 심실 및 회장 muscarinic receptor에 대한 oxomemazine의 결합성질을 조사, 비교하였다. $[^3H]QNB$ 포화결합실험 결과 세 조직의 muscarinic receptor는 $[^3H]QNB$에 대해서는 affinity가 약 60pM인 단일 receptor인 것으로 추정되었다. 대뇌에서 pirenzepine과 oxomemazine의 $[^3H]QNB$ 결합억제에 대한 Hill coefficient는 각각 0.67 및 0.8로서 대뇌에는 이들 약물에 대하여 affinity가 서로 다른 두 종류의 muscarinic receptor subtypes가 존재하는 것으로 나타났으며, pirenzepine에 대한 high $affinity(M_1)$와 $low affinity(M_2)$ receptor 및 oxomemazine에 대한 high $affinity(O_H)$와 $low\;affinity (O_L)$ receptor의 분포비는 약 60:40 및 40:60이었고, $M_1$과 $M_2$ receptor에 대한 pirenzepine의 $K_i$치는 16nM 및 431 nM, $O_H$와 $O_L$, receptor에 대한 oxomemazine 의 $K_i$치는 80nM 및 1350nM이었다. 그러나 심실과 회장에서 이들 약물의 $[^3H]QNB$ 결합억제에 대한 Hill coefficient는 1에 가까웠다. 심실과 회장 muscarinic receptor에 대한 pirenzepine의 $K_i$치는 850nM 및 250nM, oxomemazine의 $K_i$치는 1460nM 및 670nM로서 대피에서 이들 약물의 low affinity receptor에 대한 $K_i$치에 가까웠다. 즉, muscarinic receptor에 대한 affinity면에서 oxomemazine은 pirenzepine과 같이 대뇌에서 가장 높았으며, 회장에 대해서는 중등도였고, 심실에서 가장 낮았다. 이로 보아 oxomemazine은 $M_1\;receptor$에 선택성이 있는 것으로 추정된다. The binding properties of oxomemazine to muscarinic receptors using the ability of oxomemazine to inhibit $[^3H]QNB$ binding in membrane fractions of rat cerebrum and guinea pig ventricle and ileum were investigated. $[^3H]QNB$ bound to a single class of muscarinic receptors with a dissociation constant of approximately 60 pM in three tissue preparations. Pirenzepine and oxomemazine inhibited $[^3H]QNB$ binding in cerebrum with a Hill coefficient lower than unity, and the inhibition data were best described by a two-site model. The relative densities of the high $(M_1)\;and\;low\;(M_2)$ affinity sites for pirenzepine were 60 and 40%, with corresponding Ki values of 16 and 431 nM, and those $(O_H\;and\;O_L)$ for oxomemazine 40 and 60%, with corresponding Ki values of 80 and 1350 nM. However, the inhibition data of both drugs vs $[^3H]QNB$ in ventricle and ileum appeared to obey the law of mass-action (Hill coefficient close to 1). The apparent Ki values of pirenzepine were 850 and 250 nM, and those of oxomemazine 1460 and 570 nM in ventricle and ileum, respectively. Thus, oxomemazine like pirenzepine has high affinity for cerebrum, moderate affinity for ileum and low affinity for ventricle. These results suggest that oxomemazine could recognize the muscarinic receptor subtypes with a high affinity for the $M_1$ sites.

      연관 검색어 추천

      이 검색어로 많이 본 자료

      활용도 높은 자료

      해외이동버튼