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이성정 ( Sungjung Lee ),강동욱 ( Dong Wook Kang ) 대구가톨릭대학교 자연과학연구소 2015 자연과학연구논문집 Vol.13 No.1
Capsaicin is the important ingredient of hot chili pepper which is studied for pain relief, weight reduction, cardiovascular related diseases, and etc. We have prepared 7 capsaicin analogues by amide coupling reaction with EDC and HOBt. In particular, 4 analogues of them were obtained through ester coupling besides amide coupling at the same time. There were no product through only ester coupling. The more simple carboxylic acid were obtained the higher yield in ester coupling.
Fischer 에스터화 반응조건에서 아젤라익산과 같은 포화된 디카르복실산의 단일 에스터화 생성물 합성
이성정 ( Sungjung Lee ),강동욱 ( Dong Wook Kang ) 대구가톨릭대학교 자연과학연구소 2016 자연과학연구논문집 Vol.14 No.1
Fischer esterification is known to have no selectivity. However, we confirmed that monoesters of saturated dicarboxylic was obtained in general Fischer esterification condition. We were trying to react to the six saturated dicarboxylic acids in the same reaction condition, and the yields of a single esterification product were approximately 14∼26%.
신슬미,이성정,조경해,권정학,이성원,공현석,김경제 대한면역학회 2010 Immune Network Vol.10 No.2
Cordyceps militaris has been used in traditional medicine to treat numerous diseases and is reported to have antitumor and immunomodulatory activities in vitro and in vivo. The pharmacological and biochemical mechanisms of Cordyceps militaris extract (CME) on macrophages have not been clearly elucidated. In the present study, we tested the How CME induces the production of proinflammatory cytokines,transcription factor and the expression of co-stimulatory molecules was examined. Methods: We confirmed the levels of proinflammatory cytokines mRNA and protein of cytokines through RT-PCR and western blot analysis and followed by FACS analysis for the surface molecules. Results:CME increased the production of NO and proinflammatory cytokines, such as IL-1β, IL-6, TNF-α and PGE2, dose-dependently and induced protein levels of iNOS, COX-2, and proinflammatory cytokines in a concentration-dependent manner, as determined by western blot and RT-PCR analysis,respectively. The expression of co-stimulatory molecules such as ICAM-1, B7-1, and B7-2 was enhanced by CME. Furthermore, the activation of nuclear transcription factor NF-κB in macrophages was stimulated by CME. Conclusion:Based on these observations, CME increased proinflammatory cytokines through the activation of NF-κB, further suggesting that CME will provide potential use as an immune enhancing agent for treating immunological diseases.
Activation of macrophages by the components produced from Cordyceps militaris
김현희,김광희,한신하,이성정,권정학,이성원,김경제 대한면역학회 2007 Immune Network Vol.7 No.2
Background: Cordyceps militaris have been reported to modify the immune and inflammatory responses both in vivo and in vitro. Macrophages play important roles in the innate immunity through the phagocytosis of antigens. This study examined the effects of Cordyceps militaris on the activation of murine macrophage RAW 264.7 cells and primary macrophages. Methods: The components contained in culture broth of Cordyceps militaris were purified by propyl alcohol extraction and HP 20 column chromatography to CMDB, CMDBW, CMDB5P, and CMDB25P. The amounts of nitric oxide (NO) were determined by using ELISA, Griess reagent respectively. The amounts of some cytokines were determined by using ELISA, western blot, and RT-PCR The expression levels of cell surface molecules (ICAM-1, B7-1 and B7-2) were measured by flow cytometric analysis. Results: All the components of Cordyceps militaris produced significant amounts of NO. In particular, CMDB produced much more NO in RAW 264.7 cells and primary macrophages than other fractions of Cordyceps militaris. CMDB increased significantly the production of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 dose-dependently in RAW 264.7 cells. Examination of the gene expression level also showed that the enhanced production of cytokines was correlated with the up-regulation of i-NOS expression, cycloxygenase (COX)-2 expression, IL-1β and IL-6 expression, and TNF-α expression on the expression of mRNAs by semi-quantitative RT-PCR Western blot analysis also confirmed that CMDB enhances the expression level of these cytokines. Conclusion: These results show that CMDB stimulates the production of NO and pro-inflammatory cytokines and can also up-regulate the gene expression levels in macrophages.