석고를 이용한 한국재래산양 시삭상핵과 방실핵의 입체적 재구성

이봉희,이흥식,이인세,이성준,Lee, Bong-hee,Lee, Heungshik S,Lee, In-se,Yi, Seong-joon 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2

This study was carried out to reconstruct three-dimensional plaster model of the supraoptic and paraventricular nuclei of 3 Korean native goats. The representative coronal sections of the hypothalami were stained immunohistochemically with monoclonal antibodies to vasopressin and oxytocin simultaneously. Plaster models were reconstructed by schematic drawings which were made by tracing onto the tracing paper with the aid of a drawing attachment. The results were as follows: The configurations of the models of 3 supraoptic nuclei were slender spherical shape at their cranial parts, and the highest and widest size at middle parts, and became lower and narrow at caudal parts in two models, hence one was directed dorsolaterally. The medial surfaces of the para ventricular nuclei were vertically flat, and lateral surfaces were more complex than medial with processes directed dorsolaterally at their cranial portion. They change positions dorsally at caudal portion, and there were no significant variations in shape between them.

생체밖에서 미국흰불나방 지방세포에 의한 저장단백질의 흡수와 축적에 관하여

이봉희,김관선,문명진,김우갑,Lee, Bong-Hee,Kim, Kwan-Seon,Moon, Myung-Jin,Kim, Woo-Kap 한국현미경학회 1988 Applied microscopy Vol.18 No.2

This study was carried out to examine in vitro first whether the storage proteins, which the fat bodies of last larvae from Hyphantria cunea secrete into haemolymph, can be uptaked by the fat body cells of prepupa and then how the uptaked storage proteins can be accumulated in the fat body cells, if uptaken. The fat bodies which had been isolated from last instar larvae were cultured in 1 ml of Grace's insect medium containing $50{\mu}l$ of $^{3}H$-leucine (5.0 mCi/mol, Dupont) at $28{\pm}2^{\circ}C$ for 6 hrs. After the homogenates of the cultured fat bodies were centrifuged at 10,000 rpm for 10 minutes, the proteins included in the supernatant were separated by polyacrylamide gel electrophoreses (non-SDS, 6%). The next treatment of the electrophoresed gel was followed by rinsing. A storage protein band of several bands in the rinsed gel was sliced off. With elution of sliced storage protein bands in Tris-glycine buffer, the purification of radioactive storage proteins from fat bodies was finished. After the purified radioactive storage proteins were added in Grace's insect midis containing fat bodies of the prepupae, they were cultured for the randomly following minutes given as 3, 5, 7, 10, 15, 20 and 30 and for the randomly following hours given as 1, 2, 3 and 4 respectively. The double fixations of the cultured fat bodies in aldehyde and $OsO_4$, were followed by preparation of ultrathin sections from Epon-Araldite blocks through dehydration and embedding. The electron microscope autoradiographic treatment of all prepared sections were performed by the dipping method (Kim et al., 1987). The finally prepared specimens were examined with electron microscope. The fat body cells of the prepupa could be found to uptake the storage preteins of the last instar larvae, which were included in the culture medium, mostly by formation of coated vesicles. The in vitro uptake of the storage proteins actively occurred by 30 minutes after the addition of purified storage proteins in the culture medium. After culture for 7 minutes with the storage proteins, the uptaked radioactive storage proteins labelled a number of lysosomal granules. After culture for 20 minutes with the storage proteins, the radioactive storage proteins were finally incorporated and accumulated in lipid droplets and protein granules. The frequency in the fat body cell of radiolabelled lipid droplets occurs approximately 60%, while the frequency, in which the radiolabelled protein granules occurs in a fat body cell, is approximately 40%.

책머리 - 하천에 물을 돌려주자

이봉희,Lee, Bong-Hui 한국하천협회 2013 하천과 문화 Vol.9 No.3

배추흰나비 (Pieris rapae L.) 뇌간부(腦間部)의 신경세포(神經細胞)에 대한 미세구조(微細構造)

이봉희,김우갑,Lee, B.H.,Kim, W.K. 한국현미경학회 1982 Applied microscopy Vol.12 No.2

The study on the nerve cells in the pars intercerebralis(IP) of 5-day-old cabbage butterfly Pieris rapae L. was performed to observe their ultrastructures and classify them on the basis. of the differences in size, shape and relative distribution cf cell organelles. The brain-subesophageal ganglion complex was fixed in 1% paraformaldehyde-1% gluaraldehyde mixture and embedded in araldite mixture. The transverse thin sections of IP were stained with uranyl acetate and lead citrate and examined by Hitachi 500 and ]EM 100B electron microscope. Five distinct types. of nerve cells are recognized and are arbitrarily designated as Type I, Type II Type III, Type IV and Type V. Type I neurone: These neurones are neurosecretory cells. Several neurosecretory cells are. recognized in the pars intercerebralis. They are roughly round or peach-shaped cells measuring $13{\sim}25{\mu}m$ in diameter. The rounded nucleus shows about $5{\sim}10{\mu}m$ in diameter. The chromatin is predominantly diffused with only occasional dense patches. The perikaryon contains numerous. mitochondria, free polyribosomes and neurosecretory granules. The neurosecretory granules are relatively uniform in electron density, and each one is about $100{\sim}400{\mu}m$ in diameter and surrounded by a single membrane. The granules are also observed mostly as in groups. In one group of neurones the cisternae of endoplasmic reticulum are distended or in other group of neurones are not distended. Golgi saccules are slightly dilated at their lateral extremities and contains. frequenty dense rounded materials. Type II neurone: Thes have the largest soma in the pars intercerebralis about $30{\sim}35{\mu}m$ in diameter. They also show roughly polygonal in shape. The nucleus is elongated or sickle-shaped. The chromatin is mainly in the euchromatin form. The perikarya in these cells are well populated with populated with free ribosomes and contain numerous mitochondria and Golgi bodies. The cisternae of granular endoplasmic reticulum are also well distributed. Type III neurone: They are oval or spindle-shaped and also medium-sized. neurones approximately $15{\sim}17{\mu}m$ in length. The nucleus is oval or slightly elongated in shape and $8{\sim}9{\mu}m$ in length. The chromatin occurs in diffused form. The cytoplasm contains many filamentous or oval mitochondria. The perikaryon has also numerous free polyribosomes and cisternae of granular endoplasmic reticulum. Type VI neurone: They are roughly polygonal in shape probably due to the close approximation of the adjacent cells. The soma is about $7{\sim}8{\mu}m$ in diameter. The nucleus is round or oval in shape and $5.0{\sim}5.8{\mu}m$ in diameter. The necleus also occupies a large proprion of the cell body. The perikaryon is well populated with free ribosomes and contains several mitochondria and cistenae of granular endoplasmic reticulum. Type V neurone: These neurones are similar to Type VI neurones in various respects such as cell size and cell inclusion, but they differ from Type IV neurones in shape. The soma is oval or slightly elongated. The cell body contains several filamentous and oval mitochondria.

Molecular Taxonomy of a Soil Actinomycete Isolate, KCCM10454 Showing Neuroprotective Activity by 16S rRNA and rpoB Gene Analysis

이봉희,Hong Kim,Hyun-Ju Kim,Yoon-Kyu Lim,Kyung-Hee Byun,Brian Hutchinson,김창진,Young-Hwan Ko,Keun-Hwa Lee,Chang-Yong Cha,Yoon-Hoh Kook,Bum-Joon Kim 한국미생물학회 2005 The journal of microbiology Vol.43 No.2

Epilepsy constitutes a significant public health problem, and even the newest drugs and neurosurgical techniques have proven unable to cure the disease. In order to select a group of isolates which could generate an active compound with neuroprotective or antiepileptic properties, we isolated 517 actinomycete strains from soil samples taken from Jeju Island, in South Korea. We then screened these strains for possible anti-apoptotic effects against serum deprivation-induced hippocampal cell death, using the 3-(4, 5-dimethylthiazol-2-yl)2,5-diphenyl-tetrazolium bromide (MTT) assay as an in vitro test. The excitotoxic glutamate analog, kainic acid (KA), was used to induce seizures in experimental mice in our in vivo tests. As a result of this testing, we located one strain which exhibited profound neuroprotective activity. This strain was identified as a Streptomyces species, and exhibited the rifampinresistant genotype, Asn(AAC)442, according to the results of 16S rRNA and rpoB gene analyses

국산공기압축기의 품질분석과 향상에 대한 연구

이봉희,허선범,서명수,Lee, Bong-Hui,Heo, Seon-Beom,Seo, Myeong-Ju 한국기계연구원 1982 기계연구원소보 Vol.9 No.-

베트남 하천탐방기

이봉희,Lee, Bong-Hui 한국하천협회 2007 하천과 문화 Vol.3 No.1

무아레 간섭계를 이용한 유연 솔더와 무연 솔더 실장 WB-PBGA 패키지의 열-기계적 변형 거동

이봉희,김만기,주진원,Lee, Bong-Hee,Kim, Man-Ki,Joo, Jin-Won 한국마이크로전자및패키징학회 2010 마이크로전자 및 패키징학회지 Vol.17 No.3

반도체 패키지에 사용되고 있는 유연 솔더는 환경 보호 필요성 대문에 무연 솔더로 빠르게 대체되고 있다. 이와 같은 무연 솔더에 대한 여구는 주로 재료의 발견과 공정 적응성의 관점에서 이루어졌을 뿐, 기계적인 성질이나 신뢰성의 관점에서의 연구는 많이 이루어지지 않았다. 본 논문에서는 무아레 간섭계를 이용하여 유연 솔더와 무연 솔더 실장 WB-PBGA 패키지 결합체의 온도변화에 대한 열-기게적 거동을 해석하였다. 실시간 무아레 간섭계를 이용하여 각 온도 단계에서 변위 분포를 나타내는 간섭무늬를 얻고, 그로부터 유연과 무연의 솔더 조인트를 갖는 WB-PBGA 패키지의 굽힘 변형 거동 및 솔더 볼의 변형률을 비교 분석하였다. 분석결과를 보면 유연 솔더 실장 패키지 결합체의 솔더 볼은 칩경계 부근인 #3 솔더 볼에서 발생하는 전단변형률이 파손에 큰 영향을 미치며, 무연 솔더가 실장된 패키지 결합체의 솔더 볼은 가장 바깥 부근인 #7 솔더 볼에서 발행하는 수직 변형률이 파손에 큰 영향을 미칠 것으로 예측된다, 또한 무연 솔더 실장 패키지 결합체는 같은 온도 조건에서 유연 솔더 실장된 패키지에 비해 굽힘 변형이 휠씬 크게 발생될 뿐 아니라 솔더 볼의 유효변형률도 10% 정도 크게 발생하는 것으로 나타나서 열변형에 의한 파손에 취약할 것으로 예측된다. Pb-Sn solder is rapidly being replaced by lead-free solder for board-level interconnection in microelectronic package assemblies due to the environmental protection requirement. There is a general lack of mechanical reliability information available on the lead-free solder. In this study, thermo-mechanical behaviors of wire-bond plastic ball grid array (WB-PBGA) package assemblies are characterized by high-sensitivity moire interferometry. Experiments are conducted for two types of WB-PBGA packages that have Pb-Sn solder and lead-free solder as joint interconnections. Using real-time moire setup, fringe patterns are recorded and analyzed for several temperatures. Bending deformations of the assemblies and average strains of the solder balls are investigated and compared for the two type of WB-PBGA package assemblies. Results show that shear strain in #3 solder ball located near the chip shadow boundary is dominant for the failure of the package with Pb-Sn solder, while normal strain in #7 most outer solder ball is dominant for that with lead-free solder. It is also shown that the package with lead-free solder has much larger bending deformation and 10% larger maximum effective strain than the package with Pb-Sn solder at same temperature level.

특별기사 1 - 아시아 하천복원네트워크(ARRN)

이봉희,Lee, Bong-Hui 한국하천협회 2011 하천과 문화 Vol.7 No.2

사상체질에 따른 감정표현불능 성향, 반추사고, 우울감 및 신체화증상의 비교 분석: 거제지역에 거주하는 일반인을 대상으로

이봉희,이우경,Lee, Bong-Hee,Lee, Woo-Kyeong 사상체질의학회 2013 사상체질의학회지 Vol.25 No.4

Objectives This study was to examine the differences on alexithymia, rumination, depression and somatic symptoms by each Sasang constitution, and aimed at combining the psychological construct with Korean medical treatment. Methods One hundred eighty men and women aged from 20 to 69 who reside in Geoje-si were selected, and data from 173 people consisting of the three constitutions-Soyangin(39), Taeeumin(83), and Soeumin(51), excepted the minority sample of Taeyangin(7 people)-were analyzed. Collected data were analyzed and tested by IBM SPSS Statistics 20: frequency analysis for demographic data; t-test for gender differences; one-way ANOVA for differences among three constitutions; and Pearson correlation analysis for the correlation between variables. Results The result of t-test taken for the gender differences between variables was not significant. According to the result of one-way ANOVA conducted by different Sasang constitutions, no variables showed a significant result except that of alexithymia. The correlation analysis showed that the alexithymia had positive correlation with rumination, depression, and somatic symptoms. Rumination was in positive correlation with depression and somatic symptoms. Especially, depression had high positive correlation with somatization symptoms. Among the variables analyzed by each constitution, only the survey result of the alexithymia was significant. Conclusions Given that each constitution showed different levels of the alexithymia, it can be concluded that therapeutic intervention in the treatment for the Taeeumin who shows a high level of alexithymia is needed to improve their emotional recognition and expression. In this context, application of program which focus on the emotional ability such as emotion coaching to the Korean medical treatment is expected to improve alexithymia, depression, and somatic symptoms.