망막-시상하부 경로의 광반응 특성

이배환 ( Bae Hwan Lee ),손진훈 ( Jin Hun Sohn ),( Shigeki Watanuki ),( Akira Yasukouchi ),( Takeshi Morita ) 한국감성과학회 2007 감성과학 Vol.10 No.2

좌골신경 손상 후 기능회복에 미치는 acidic fibroblast growth factor의 효과

이배환 ( Bae Hwan Lee ),김은정 ( Eun Jung Kim ),이경희 ( Kyung Hee Lee ),정세정 ( Se Jung Jung ),박용구 ( Yong Gou Park ) 한국심리학회 2002 한국심리학회지 생물 및 생리 Vol.14 No.1

좌골신경 손상 후 Nitric oxide synthase inhibitor의 주입에 의한 기능회복 촉진 효과

이배환 ( Bae Hwan Lee ),원란 ( Ran Won ),김은주 ( Eun Joo Kim ),남혜윤 ( Hye Yoon Nahm ),김은정 ( Eun Jung Kim ),박용구 ( Yong Gou Park ) 한국심리학회 2002 한국심리학회지 생물 및 생리 Vol.14 No.1

척수손상 후 세포이식에 의한 운동기능의 회복증진

이배환 ( Bae Hwan Lee ),이경희 ( Kyung Hee Lee ),성제경 ( Je Kyung Seong ),황세진 ( Se Jin Hwang ),김계성 ( Kye Seong Kim ) 한국감성과학회 2004 감성과학 Vol.7 No.2

흰쥐에서 체감각유발장전위의 기록부위별 특성과 경로분석

신현철,박용구,이배환,류재욱,조춘식,정상섭,Shin, Hyun Chul,Park, Yong Gou,Lee, Bae Hwan,Ryou, Jae Wook,Zhao, Chun Zhi,Chung, Sang Sup 대한신경외과학회 2001 Journal of Korean neurosurgical society Vol.30 No.7

Objective : Somatosensory evoked potentials(SSEPs) have been used widely both experimentally and clinically to monitor the function of central nervous system and peripheral nervous system. Studies of SSEPs have reported the various recording techniques and patterns of SSEP. The previous SSEP studies used scalp recording electrodes, showed mean vector potentials which included relatively constant brainstem potentials(far-field potentials) and unstable thalamocortical pathway potentials(near-field potentials). Even in invasive SSEP recording methods, thalamocortical potentials were variable according to the kinds, depths, and distance of two electrodes. So they were regarded improper method for monitoring of upper level of brainstem. The present study was conducted to investigate the characteristics of somatosensory evoked field potentials(SSEFPs) of the cerebral cortex that evoked by hindlimb stimulation using ball electrode and the pathways of SSEFP by recording the potentials simultaneously in the cortex, VPL nucleus of thalamus, and nucleus gracilis. Methods : In the first experiment, a specially designed recording electrode was inserted into the cerebral cortex perpendicular to the cortical surface in order to recording the constant cortical field potentials and SSEFPs mapped from different areas of somatosensory cortex were analyzed. In the second experiment, SSEPs were recorded in the ipsilateral nucleus gracilis, the contralateral ventroposterolateral thalamic nucleus(VPL), and the cerebral cortex along the conduction pathway of somatosensory information. Results : In the first experiment, we could constantly obtain the SSEFPs in cerebral cortex following the transcutaneous electrical stimulation of the hind limb, and it revealed that the first large positive and following negative waves were largest at the 2mm posterior and 2mm lateral to the bregma in the contralateral somatosensory cortex. The second experiment showed that the SSEPs were conducted by way of posterior column somatosensory pathway and thalamocortical pathway and that specific patterns of the SSEPs were recorded from the nucleus gracilis, VPL, and cerebral cortex. Conclusion : The specially designed recording electrode was found to be very useful in recording the localized SSEFPs and the transcutaneous electrical stimulation using ball electrode was effective in evoking SSEPs. The characteristic shapes, latencies, and conduction velocities of each potentials are expected to be used the fundamental data for the future study of brain functions, including the hydrocephalus model, middle cerebral artery ischemia model, and so forth.

백서에서 삼차신경 유발전위의 특성과 경로 분석

김세혁,조춘식,권오규,이배환,박용구,정상섭,Kim, Se-Hyuk,Zhao, Chun-Zhi,Kwon, Oh-Kyoo,Lee, Bae-Hwan,Park, Yong-Gou,Chung, Sang-Sup 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.8

Objective : There are some advantages of trigeminal evoked potential(TEP) recording compared to other somatosensory evoked potential(SSEP) recordings. The trigeminal sensory pathway has a pure sensory nerve branch, a broader receptive field in cerebral cortex, and a shorter pathway. Despite these advantages, there is little agreement as to what constitutes a normal response and what wave forms truly characterize the intraoperative TEP. This study presents the normative data of TEP recorded on the epidural surface of the rat with a platinum ball electrode. Materials & Methods : Under general anesthesia with urethane, the adult Sprague-Dawley male rats(300-350g) were given electrical stimulation with two stainless steel electrodes which were inserted into the subcutaneous layer of the area around whiskers. A reference electrode was positioned in the temporalis muscle ipsilateral to the recording site. Results : TEPs were recorded in the Par I area of somatosensory cortex and recorded most apparently on the point of 2mm posterior from the bregma and 6mm lateral from the midline. The typical wave form consisted of 5 peaks (N1-P1-N2-P2-N3 according to emerging order, upward negativity). Each latency to corresponding peaks was not influenced by the different intensities of stimulation, especially from 1 to 5mA. Average latencies of 5 peaks were in the following order ; 7.7, 11.1, 15, 22.3, 29.4ms. There was also no significant difference between latencies before and after administration of muscle relaxant(pancuronium). For the electrophysiological localization of recorded waves, the action potential of a single unit was recorded with glass microelectrode(filled with 2M NaCl, $3-5M{\Omega}$) in the thalamus of rat. A sharp wave was recorded in the VPM nucleus, in which the latency was shorter than that of N1. This suggests that all 5 peaks were generated by neural activities in the suprathalamic pathway. Conclusion : In terms of recording near-field potentials, our data also suggests that TEP in the rat may be superior to other SSEPs. In overall, these results may afford normative data for the studies of supratentorial lesions such as hydrocephalus or cerebral ischemia which can have an influence on near-field potentials.

성별에 따른 향 선호도 결정에 미치는 주관적 감성요인

백은주 ( Eun Joo Baik ),이윤영 ( Yun Young Lee ),김완석 ( Wan Suk Gim ),이배환 ( Bae Hwan Lee ) 한국감성과학회 1998 추계학술대회 Vol.1998 No.-

향은 방향성 또는 휘발성 물질에 의해 코의 점막을 통해 흡입되어 여러 감성의 변화와 뇌파등 생체 신호의 변화를 초래하고 또한 풍부한 감성도 유발한다. 특히 기억력에 관련된 원시 감정을 유발하고 생체에서 나오는 phermone(페르몬)은 행동 양식까지 영향을 미친다. 또한 질환치료에도 적용되거나 증상의 경감, 예방효과 등을 보여주는 aromatherapy는 매우 유망한 분야이다. 본 연구에서는 이러한 페르몬 향과 aromatherapy에 사용되는 essential oil등을 이용하여 주관적 감성 평가를 실시하고 이를 통계분석하여 이들 여러 종류의 향들의 자극으로 유발되는 감성의 특징을 밝혀내고자 하였다. 둘째로 향의 선호도를 결정하는데 어떠한 감성요인이 작용하는지를 알고자 하였다. 실험에 사용된 향은 26종류이고 총 33명의 지원자가 참여하였다. 설문지는 18문항을 선별하여 4가지 유형으로 작성한 후 유형별로 데이타를 정리하여 베리막스의 요인분석, 회귀분석, 군집분석등을 이용하여 분석하였다. 베리 막스의 요인분석으로 감성 형용사를 비슷한 척도끼리 묶어 괘한 감성을 결정하는 요인, 자극의 강도를 결정하는 요인, 이외의 다른 고풍스럽거나 현대적인 요인등 3개 요인으로 grouping하였다. 향의 선호도를 결정하는데 중요한 감성척도에서는 피검자 성별에 따라 차이가 있음을 보여주었다. 남성의 경우 황홀하다, 여성적이다인데 반해 여성인 경우 쾌적하다, 친숙하다라는 감성 척도가 중요하게 작용하였다. 또한 각 향별로 군집 분류하여 향의 종류를 구분할 수 있었으며 또한 각 향별 선호도를 결정하는 감성 척도도 구해 보았다. 또한 향의 분류에 따른 감성 척도의 특징을 성별로 관찰한 결과 페르몬 향인 경우 essential oil과 달리 성별의 차이가 나타났다. 결론적으로 후각을 자극하는 향물질로 유발된 감성 측정을 해 본 결과 성별에 따라 선호도를 결정하는 감성요인의 차이를 알 수 있었으며 또한 essential oil에서는 성별 차이가 없는데 반해 페르몬 향의 경우 성별의 차이를 나타내었다.

수두증 흰쥐 모델에서 수두증 정도에 따른 체성 감각 유발 장전위의 변화

김동석,이광수,박용구,김세혁,최중언,이배환,류재욱,Kim, Dong-Seok,Lee, Kwang-Soo,Park, Yong-Goo,Kim, Se-Hyuk,Choi, Joong-Uhn,Lee, Bae-Hwan,Ryou, Jae-Wook,Zhao, Chun-Zhi 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.1

Objective : Somatosensory evoked potential(SSEP) has been known to be a good method for evaluating brain stem function, but it is not sufficient to check the fine changes of cortical functions. A fine change of cortical function can be expressed with somatosensory evoked cortical field potential(SSEFP) rather than general SSEP. To confirm the usefulness of SSEFP for evaluating the cortical function, the authors simultaneously measured SSEFP and the intracranial pressure-volume index(PVI) in kaolin-induced hydrocephalic rats. Method : Hydrocephalus was induced with injection of 0.1ml kaolin-suspended solution into the cisterna magna in 60 Sprague-Dawley rats. The authors measured PVI and SSEFP 1 week after injection of kaolin-suspended solution. To evaluate the severity of induced hydrocephalus, we measured the transverse diameter of the lateral ventricle on the coronal slice of the rat brain 0.40mm posterior to the bregma. Result : The typical wave form of SSEFP in control rats showed a negative-positive complex wave at early latency. In SSEFP of normal rats, N0 is 10.0 msec, N1 15.3 msec, P1 31.2 msec and N1-P1 amplitude $15.4{\mu}V$. As hydrocephalus progressed, the peak latency of N1 and P1 were delayed. In mild hydrocephalus, negative peak waves were split. The N1-P1 amplitude was decreased only in severe hydrocephalus. The changes of the characteristics of SSEFP according to the severity of hydrocephalus were well correlated with the changes of PVI. Shunting normalized the characteristics of SSEFP in relation to ventricular sizes and PVI in hydrocephalic rats. Conclusion : SSEFP may be useful for evaluating the impairment of cortical function in hydrocephalus.

Mannitol의 주입 경로와 양 및 농도가 혈-뇌 장벽 손상에 미치는 효과

차명훈 ( Myeoung Hoon Cha ),이규홍 ( Kyu Hong Lee ),이철현 ( Chul Hyun Lee ),정명애 ( Myung Ae Chung ),손진훈 ( Jin Hun Sohn ),정재준 ( Chae Joon Cheong ),이배환 ( Bae Hwan Lee ) 한국감성과학회 2008 감성과학 Vol.11 No.4

Functions of human brain including sensibility and emotion may be affected by drugs mediated by the blood-brain barrier (BBB). The present study was performed to evaluate whether injection route, volume and concentration of mannitol could alter the degree of disruption of the BBB. Under urethane anesthesia, female Sprague-Dawley rats were infused with 20% mannitol into the right internal carotid artery (ICA). In the other group, intravenous injection of mannitol through the femoral vein was performed. Evans blue (EB) dye was used as a marker of BBB disruption. When mannitol was injected via the ICA, the content of EB dye in the ipsilateral hemisphere was markedly increased. However, the content of EB in the brain was not increased when mannitol was injected via the femoral vein, even though the volume or concentration of mannitol was increased. These results suggest that the BBB was disrupted only through ICA injection route and this may provide a useful strategy for transient opening of the BBB to control the functions of human brain.

Lavendar와 Jasmin으로 유발된 후각 강성에 대한 중추 및 자율신경계 반응

백은주 ( Eun Joo Baik ),이윤영 ( Yun Young Lee ),하태환 ( Tae Hwan Hah ),임재중 ( Jae J Im ),이배환 ( Bae Hwan Lee ) 한국감성과학회 1998 추계학술대회 Vol.1998 No.-

향에 의해 유발되는 감성에 대한 중추신경계 및 자율신경계의 반응의 변화를 측정하기 위해 안정시키는 향과 각성시키는 향을 사용하여 주관적 평가와 동시에 시행하였다. 안정시키는 향으로 1% Lavendar 향을 사용하였고, 각성시키는 향으로 0.8% Jasmin 향을 사용하였으며, 안정상태를 향자극 전후에 측정하여 대조군으로 사용하였다. 중추신경계의 지표로 뇌파측정을 하였고 뇌파의 전극은 international 10-20 system에서 4 채널을 사용하였으며, 자율신경계의 지표로는 심전도, heart rate, 피부저항, 피부온도를 기록하였다. 뇌파의 분석은 Fast Fourier Transform analysis의 power spectra로 하였고, 그 frequency bands는 theta(4-8Hz), alpha(8-l3Hz), beta(14-30Hz)로 하였다. 또한 심전도를 이용하여 심전도 상의 연속적인 R-R peak간 시간간격을 시계열 데이터로 재구성한 Heart rate variability 분석도 하였다. HRV 분석을 보다 정확히 할 수 있도록 호흡이 심전도에 미치는 영향을 제거하기 위하여 호흡을 분당 20회로 일정하게 하였다. 생체신호 측정과 동시에 실시한 주관적 검사에서 lavendar 향은 친숙하게, jasmin 향은 활기차고 상쾌하고 유쾌하게 평가되었다. 뇌파 분석에서 lavendar 향을 주었을 때 theta의 증가 양상을 보였으며, Jasmin 향을 주었을 때는 모든 채널에서 beta 파의 증가 양상을 보였다. 또한 HRV 분석 결과 부교삼신경의 활동성이 부각되는 HF/LF의 값이 lavendar에서는 대조 자극보다 높게 나타났으며, jasmin에서는 대조자극보다 낮은 값이 나타나는 경향을 보였다. 결론적으로 안정과 각성의 후각 자극으로 인한 감성의 변화를 뇌파와 자율신경계 등의 생체지표로 관찰할 수 있었다.