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지렁이 중장에서 발현되는 Endo-$\beta$-1,4-glucanase의 동정 및 특성에 관한 연구
이명식,조성진,탁은식,허소영,이종애,박범준,조현주,신주옥,박순철,Lee Myung Sik,Cho Sung Jin,Tak Eun Sik,Hur So Young,Lee Jong Ae,Park Bum Joon,Cho Hyun Ju,Shin Chuog,Park Soon Cheol 한국토양동물학회 2003 한국토양동물학회지 Vol.8 No.1,2
지렁이 (Eisenia andrei)의 중장에서 내생의 endoglucanase 유전자의 전체 염기 서열을 동정하였다. ORF의 길이는 1,371 bp이며, 456개의 아미노산으로 번역된다. NCBI에 등록된 가재와 흰개미의 cellulase 및 endo-$\beta$-1, 4-glucanase와 50-51%의 유사성을 보이며, 활성 부위가 잘 보존되어 있었다. 계통수 분석에서는 다른 동물 분류군에서 밝혀진 GHF9 그룹의 cellulase와 근연관계가 없음이 확인되었다. Endogeneous endoglucanase (EC 3.2.1.4) cDNA was cloned from a representative species (Eisenia anderi) of the earthworm family Lumbricidae. Endoglucanase from the midgut of the earthworm is composed of 456 amino acids and belongs to glycosyl hydrolase family 9 (GHF9), sharing high homologies (50-51 %) with those of selected termite and crayfish. This endoglucanase consists of three consensus catalytic domains found in most microbial cellulases. A phylogenetic tree was constructed using the amino acid squence data matched through the BLASTX program and showed that GHF9 families could be divided into four groups of arthropoda, bacteria, plant and annelida.
이명식(Myung Sik Lee) 대구사학회 2001 대구사학 Vol.65 No.1
It might be at the age of Queen Chinseong that the present 5eongju District began to be freed from the rule of the Central Government. And then, it might be inferred, when Later Packjae attacked and occupied 10 castles or so south of II-sung Commencery at the 11th year of King Hyogong, that Seongju began to be influenced by Later Packjae. At that time, however, Later Packjae was unable to keep on holding the territory which it took from Sillla, so the present Seongju District was ruled by the lord who extended his power and called himself a General. After that, it is obvious that Seongju District was influenced againby Later Packjae from about 4th year of King Kyoungmyoung(920 A.D.), equal to the period when Koryeo and Later Packjae was in the rivals each other. But Seongju became to be under the rule of Korea, aftert Ryangmun, General of Pyuckjin Comrnendery, yielded to Koryung in the ?th year of King Kyoungmyoung(924). In the first year of King, Kyoungae(924), Koryeo and Later Packjae committed a battle for a long period at Jomoo Castle, and General Lee in Later Packjae, local gentry in Seongju District, yielded to Koryeo, which turned the battle favorable to Koryeo. After the Battle of Gongsan, offering a route of retreat to King, Wang Keon who lost in the battle, he contributed much to King, Wang Keon, On one hand, when Lee Chong-eon, General of Pyuckjin Commendery, fought against Koryeo at the Battle of Chomul Castle, his son, Lee Young joined in the battle and served greatly to gain victory for Koryeo. King, Wang Keon rewarded him far his distinguished services at the battle, and appointed him as General Boneup and as Samjungdaekwang. What Lee Neung-il had achieved, can be revealed from a lot off history books written at the middle period of Lee Dynasty, but his name, life and achievements are not written in the Samgaoksaki and Koryeosa which are the basic historical materials. Therefore, the writer concluded that Lee Neung-il might be considered as Lee Neung-mun who can be traced and identified from the basic historical documents, who yielded to Koryeo in the second year of King Kyoungae(925) and was appointed as Seangsangun and ruled Kyaungsan Commendery.
이명식(Lee Myung-Sik) 대한건축학회 2009 대한건축학회논문집 Vol.25 No.2
People are getting information for building through various medium. But people can not approach the required data in spite of necessity and heft of performance information of building grade. Because synthetic evaluation model and systematic computing system for performance information of building is not consisting in domestic circumstance. That is why this paper is intended to propose computer based implementation model for Building Performance Evaluation. This research suggests (1)the criteria of performance evaluation items to be divided into two categories - performance of building itself, performance of exterior effect on building (2)the implementation scheme of web-based building performance evaluation system through computer aided model and the information classification system for the building performance evaluation.
신용카드사의 웹사이트 서비스품질이 e-로열티 및 가상공동체의식에 미치는 영향
이명식(Lee, Myung Sik) 한국서비스경영학회 2016 서비스경영학회지 Vol.17 No.2
The purpose of this study is to examine the effect of website service quality of credit card company on e-loyalty and a sense of community as well as a mediating effect of e-loyalty on relationship between website service quality and a sense of virtual community, and a moderating effect of perceived security risk on relationship between website service quality and e-loyalty. To measure website service quality, four elements are suggested as follows: communication, system operation, aesthetics, and exchange process. Five hypotheses are proposed and tested empirically. All of elements of website service quality have a positive effect on e-loyalty. Also, three element except aesthetics have a positive effect on a sense of virtual community. There are mediating effects of e-loyalty on relationship between all of elements of website service quality and a sense of virtual community, and moderating effects on both relationship between system operation and e-loyalty and relationship between exchange process and e-loyalty. Further theoretical and managerial implications as well as research limitations are discussed.