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이준범,Lee Byoung-hee,박정준,Hong Jeong In,Lee Dae-Weon 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.3
The tobacco whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae), is a serious pest that transmits tomato yellow leaf curl virus (TYLCV) in tomato. The tobacco whitefly exhibits morphological similarity with the greenhouse whitefly, Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) which also damages various plants in the green house. Therefore, it is very important to quickly and accurately diagnose whiteflies for applying pest manage ment strategies. In this study, we used a loop-mediated isothermal amplification (LAMP) assay to quickly and effectively detect B. tabaci. Primer sets were investigated for the specificity of B. tabaci by visible detection of the target DNA fragment. Two primer sets for pheromone biosynthesis activating neuropeptide (PBAN) gene were obtained from transcriptomic analysis of B. tabaci biotype Q. The PBAN-based LAMP primer set showed specific amplification of the target region. The optimal conditions for B. tabaci detection were 60 ◦ C for 60 min with four LAMP primers of PBAN. The minimum amount of genomic DNA required for visible detection was 100 ng. These results suggest that the PBAN-based LAMP assay can be applicable for field monitoring of B. tabaci.
이연수 ( Y S Lee ),이명구 ( M G Lee ),김용곤 ( Y G Kim ),백신원 ( S W Paik ),송창근 ( C G Song ),한대희 ( D H Han ),오태근 ( T K Oh ) 한국안전학회(구 한국산업안전학회) 2014 한국안전학회지 Vol.29 No.2
The construction accidents in temporary structures have ceaselessly happened and these mostly lead to serious disasters associated with public criticism. Recently, the accidents under construction due to incomplete or faulty design has repeatedly occurred such as the overturned girder accident in Jangnam Bridge, the submerged incident in Noryangjin and the slab collapse in Banghwa Bridge. In order to prevent such accidents due to the faulty design in temporary structures, it’s important to set up the solid construction management system which allows the reasonable design change if necessary. In this regard, this study provides the basic data for the reasonable design change in temporary structures by conducting a question investigation to the construction, design, and supervising companies. From the survey results, the kind and range of the temporary structures which should considers the design change were suggested by the deduced reasonable processes.
오태근 ( T K Oh ),김용곤 ( Y G Kim ),이명구 ( M G Lee ),백신원 ( S W Paik ),우인성 ( I S Woo ),송창근 ( C G Song ) 한국안전학회(구 한국산업안전학회) 2014 한국안전학회지 Vol.29 No.1
Article 29(3) of Occupational Safety And Health Act, which states a contractor can request a design change to an employer of businesses under the risk condition of construction of temporary structure, was established. Accordingly, in this study, recent fatal accidents caused by temporary structures were analyzed, and the level of inclusion of temporary structures in the design document were examined, and high risk temporary structures were classified. In addition, the requirements of design change of temporary structures were presented, and the qualifications of expert to certify the design change were proposed