한국인 기관지 천식 허증(虛證), 실증(實證) 환자와 CD46 유전자 다형성과의 관계

이매,백현정,박의근,김관일,이범준,김수강,정주호,김진주,김미아,정희재,정승기,Lee, Mei,Baek, Hyun-jung,Park, Eui-keun,Kim, Kwan-il,Lee, Beom-joon,Kim, Su-kang,Chung, Joo-ho,Kim, Jin-ju,Kim, Mi-a,Jung, Hee-jae,Jung, Sung-ki 대한한방내과학회 2015 大韓韓方內科學會誌 Vol.36 No.4

Objectives In this study, we divided Korean asthma patients into excess syndrome or deficiency syndrome groups according to clinical phenotype. Genetic analysis was conducted to investigate the association of exonic SNPs in the CD46 gene polymorphism with the clinical phenotype based on the differentiation syndrome of the bronchial asthma patients.Methods There were 95 healthy patients (control group) and 53 asthma patients. (The deficiency syndrome group included 24 and the excess syndrome group 29). We searched the exonic areas of the CD46 gene in the NCBI website SNPs with <0.01 minor allele frequency (MAF) and <0.01 heterozygosity. We finally selected two SNPs: rs138843816, Ser13Phe and rs7144, 3’-UTR. Hardy-Weinberg equilibrium was calculated using SNPStats.Results There were significant differences in the codominant 1 model and the dominant model between the healthy group and the asthma group. There were significant differences between deficiency syndrome group and the excess syndrome group in the genotype frequencies and in the codominant 1 model, the dominant model, and the log-additive model. The allele frequency of rs7144C showed a significant difference between the deficiency syndrome group and the excess syndrome group. Two-SNP haplotype analysis showed a significant difference in frequency in the deficiency syndrome group and in the excess syndrome group. There were significant differences between the healthy group and the excess syndrome group in the codominant 1 model, the dominant model, and the log-additive model. The frequency of the rs7144 C allele exhibited a significant difference in the demonstration. SNP haplotype analysis between the healthy group and the excess syndrome group showed a significant difference in the frequency of the CT haplotype and the CC haplotype.Conclusions The results indicate that two CD46 SNPs (rs138843816, Ser13Phe and rs7144, 3′–UTR) might be associated with the symptomatic excess syndrome in Korean asthma patients.

한ㆍ중 가족드라마 서사 비교분석 - <부탁해요, 엄마>와 <둘째시대(二胎时代)>를 대상으로

이매,남태수,한혜경 동북아시아문화학회 2016 동북아 문화연구 Vol.1 No.49

The purpose of this study is to compare the conflict structures of Korean family drama(<Second Child’s Generation>) and Chinese family drama(<Second Child’s Generation>), using the semiotic method of Greimas. The results of the narrative analysis on the actantial model and the semiotic square show that in both of Korean and Chinese dramas the object which the subjects will desire to achieve is the same. It is the ‘harmony of the family.’ But the contrary terms that bring disharmony and cause conflicts among the family members are different. In Korean drama, the contrary term is ‘discrimination’, while in Chinese drama it is ‘difference’. In Korean drama, the subject is the daughter-in-law, Jin-Ae Lee. She has been discriminated against for being a woman. Her marriage was opposed by her future mother-in-law because she was poor and has not graduated outside of high school. After she succeeded in marriage, she has been ignored by her mother-in-law. She has continued to adjust to her mother-in-law until her sacrifice is recognised by other family members, especially her mother-in-law. By that she finally achieved her desire-having a harmonious family. In the Chinese drama, the subject is the wife, Chan-Chan Jin. She has the different opinions about the family issues such as parenting and livelihood with her husband and mother-in-law. She has continued to argue with them. Finally, she carried her points through with them and by that she acquired the harmonious family. So the heroine of the Chinese family drama goes farther from the existing patriarchal system.

생강감초탕(生薑甘草湯) 및 생강(生薑), 감초(甘草)가 생쥐 비장세포의 활성에 미치는 영향

이매,김진주,정희재,정승기,Lee, Mei,Kim, Jin-Ju,Jung, Hee-Jae,Jung, Sung-Ki 대한한방내과학회 2011 大韓韓方內科學會誌 Vol.32 No.3

Objectives : Zingberis Rhizoma (ZR) and Glycyrrihizae Radix (GR) have been widely used to prevent or treat allergic diseases. However, relatively little research has been conducted on the immune response to Senggang Gamcho-tang (SG) in a mixture of ZR and GR. The purpose of this study was to determine antiallergic effects of Senggang Gamcho-tang (SG) extracts ZR and GR on activity of murine splenocytes. Methods : This study was performed to investigate the effect of SG in mice, using in vitro experiments. Cells were treated with SG extract (1 ${\mu}g$/ml, 10 ${\mu}g$/ml and 100 ${\mu}g$/ml) plus Con A (2 ${\mu}g$/ml) and ZR (6.6 ${\mu}g$/ml and 66 ${\mu}g$/ml), GR (3.3 ${\mu}g$/ml and 33 ${\mu}g$/ml) respectively for 48 hrs. The production of IFN-${\gamma}$ and IL-4 were determined by ELISA. Results : No toxicity was found in splenocytes treated with SG extract for 48 hrs at the concentration of 0 ${\mu}g$/ml, 1 ${\mu}g$/ml, 10 ${\mu}g$/ml and 100 ${\mu}g$/ml, respectively. The production of IFN-${\gamma}$ increased to 23,968.7 pg/ml (p<0.001) while that of IL-4 significantly decreased to 14.1 pg/m l(p<0.05) only at the SG concentration of 100 ${\mu}g$/ml. With the GR concentration of 33 ${\mu}g$/ml in SG extract, the production of IFN-${\gamma}$ rose to 32,102.2 pg/ml (p<0.001) while that of IL-4 (p<0.05) declined to 34.2 pg/ml. However, there was no significant observation with regard to the effect of ZR on production of IFN-${\gamma}$ and IL-4. Conclusions : This study suggests an antiallergic effect of Senggang Gamcho-tang, which leads to the implication that SG extract may be used to enhance human immune function.

통영(統營) 오광대(五廣大) 대사(臺詞)

이매기 국어국문학회 1960 국어국문학 Vol.- No.22

한ㆍ중 가족드라마에 나타난 갈등구조 비교분석

이매(李梅),남태수(Taesu Nam),한혜경(Hyekyoung Han) 동북아시아문화학회 2016 동북아시아문화학회 국제학술대회 발표자료집 Vol.2016 No.10

The purpose of this study is to compare the conflict structure of Korean family drama(<Second Child’s Generation>) to one of Chinese family drama(<Second Child’s Generation>), using the semiotic method of Greimas. The results of the narrative analysis on the actantial model and the semiotic square show that both of Korean drama’s subject and Chines drama’s one desire the harmony of the family. But the contrary terms to the harmony which cause conflicts among the family members are different. In Korean drama, the contrary term is the discrimination. However, it is the difference in Chines drama. In Korean drama, the subject is the daughter-in-law. She has continued to adjust to her husband’s mother. When her sacrifice was recognized by her husband’s mother, she acquired the harmonious family. In Chines drama, the subject is the wife. She has continued to argue her husband into accepting her opinions about family affairs. Finally, she carried her points with her husband and acquired the harmonious family. So the heroine of Chines family drama goes further away from the partriarchal system.

동충하초균주로 발효한 감초의 주요성분 함량 변화 및 NO 생성 억제 효과

왕자옥(Ziyu Wang),이매(Mei Li),이커(Ke Li),손병구(Beung Gu Son),강점순(Jum Soon Kang),이용재(Yong Jae Lee),박영훈(Young Hoon Park),김선태(Sun Tae Kim),정재철(Jae-Chul Jung),이영근(Young Guen Lee),최영환(Young Whan Choi) 한국생명과학회 2017 생명과학회지 Vol.27 No.1

약용식물의 발효는 새로운 식품의 소재 개발이 가능하나, 발효 균주는 대부분 이스트, 유산균, 박테리아 등이 이용되고 있다. 본 연구에서는 감초와 번데기 단독 또는 감초에 발효원인 번데기를 20%와 50%로 첨가한 혼합물의 배지에 눈꽃 동충하초(Paecilomyces tenuipes)와 밀리타리스 동충하초(Cordyceps militaris)를 이용하여 고체배양방법을 확립하였다. 동충하초 발효물을 식품소재로 개발하기 위하여 식용 가능한 용매인 에탄올 95%, 70%, 50%, 25% 및 물로서 추출한 다음 동충하초로부터 생성된 cordycepin과 감초의 지표성분인 liquiritin, liquiritigenin과 glycirrhizin의 함량 및 NO생성 억제효과를 조사하였다. Cordycepin함량은 감초에 번데기를 50%로 혼합한 배지에 밀리타리스 동충하초 균주을 접종하여 발효한 발효물을 70% EtOH추출하였을 경우에 가장 많았으며, 번데기를 첨가하지 않은 밀리타리스 동충하초 발효물 추출물보다 함량이 33배 정도 증가하였다. 또한 추출용매의 극성이 70% EtOH보다 높거나 낮아지면 감소하는 경향이었으며, 특히 발효원으로서 번데기의 첨가는 cordycepin의 함량을 현저하게 증가시켰다. Liquiritin의 함량은 발효하지 않은 감초보다 눈꽃 동충하초와 밀리타리스 동충하초로 발효한 모든 추출물에서 감소하였다. Liquiritigenin의 함량은 눈꽃 동충하초로 발효한 추출물이 밀리타리스 동충하초 발효 추출물보다 현저히 증가하였으나, 밀리타리스 동충하초 균쥬의 발효 추출물은 발효하지 않은 감초추출물과 거의 차이가 없었으며, 두 균주 모두 번데기의 첨가량이 증가할수록 liquiritigenin의 함량이 감소하는 경향이었다. 감초에 번데기의 첨가량 또는 추출 용매의 극성이 증가하면 liquiritin과 glycyrrhizin의 함량은 현저히 감소하였다. 이상의 결과로부터, cordycepin 함량은 C. militaris 균주로 liquiritigenin은 P. tenuipes로 발효시에 현저하게 증가하였으나, liquiritin과 glycyrrhizin은 감소하였다. 감초를 동충하초로 발효시에 번데기의 첨가는 주요 성분의 변화를 현저하게 유도하였다. 동충하초 발효 추출물은 NO생성 억제효과가 증가하였으며, 고극성 용매추출물에서 그 효과가 현저하였다. 감초의 발효시에 생성된 cordycepin과 liquiritin, liquiritigenin 및 glycyrrhizin의 함량은 발효원으로서 첨가되는 번데기, 추출용매의 극성, 발효 균주의 종류 등에 따라서 현저한 차이가 있었다. 이러한 결과는 동충하초 균주를 이용한 기능성 식품 소재를 개발하기 위한 기초 자료로서 활용이 가능할 것으로 기대된다. Traditional Korean fermented herbal plants are potential sources of new food that promote health, but they are still produced by yeast, fungi or bacteria fermentation. In the present work, mushroom (Paecilomyces tenuipes and Cordyceps militaris) fungal dongchunghacho were used to fermented Glycyrrhiza uralensis Fischer (licorice) or mixed with pupa. The pupa were tested as solid substrates for the production of corcycepin, liquiritin, and liquiritigenin. The fermented substrates were analyzed the content of cordycepin, liquiritin, liquiritigenin, and glycirrhizin productivity and inhibitory activity of NO. The cordycepin content of 70% EtOH extract from the fermented mixture of licorice and 50% pupa with C. militaris increased maximum at 33 times. Pupa was very excellent for the production of cordycepin. The liquiritin content was decreased in all the assays inoculated with P. tenuipes and C. militaris dongchunghachos. The liquiritigenin content was higher when fermented with P. tenuipes than C. militaris. The addition of pupa significantly reduced the liquiritin content and glycyrrhizin production. As a result, the liquiritigenin content increased in fermented P. tenuipes and C. militaris, and liquiritin and glycyrrhizin decreased. The inhibition of NO production in the different ethanolic extracts fermented with licorice and pupa was also significantly increased and higher than that of a nonfermented extract in higher polar solvent extracts. The contents of cordycepin and biological active compounds were altered in accordance with the concentration of pupa and fungi. This study provides basic data for use in developing dongchunghacho fungi as a functional food resource.

딸기과실의 성숙시기별 생리활성성분 함량분석

고민정(Min Jung Ko),안재욱(Jae Uk An),왕자옥(Ziyu Wang),이매(Mei Li),강점순(jum Soon Kang),손병구(Beung Gu Son),이용재(Yong Jae Lee ),박영훈(Young Hoon Park),홍광표(Kwang Pyo Hong),강남준(Nam Jun Kang),최영환(Young Whan Choi) 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.5

Lipolysis Effect of Daucosterol Isolated from Mulberry (Morus alba) Leaves

Ke Li1(이커),Mi Lim Lee(이미림),Lu Que(루쿼),Mae Li(이매),Jum Soon Kang(강점순),Yung Hyun Choi(최영현),Kyung Mi Kim(김경미),Jae-Chul Jung(정재철),Dae Youn Hwang(황대연),Young Whan Choi(최영환) 한국생명과학회 2017 생명과학회지 Vol.27 No.12

뽕나무는 약 40여 속과 1,000여 종이 있는 것으로 알려져 있으며, 항염증, 항진정, 지사작용, 노화억제 및 신경보호작용이 있는 것으로 알려져 있다. 본 연구에서는 아시아 지역에서 전통 한약재로 사용되는 뽕 나무 잎의 지방분해 활성에 관여하고 있는 물질을 스크린하기 위하여 뽕잎 분말을 헥산, 에틸 아세테이트 및 메탄올로 순차 추출하였다. 뽕 잎의 EtOAc 추출물로부터 daucosterol이 순수분리 되었으며, 그 구조는 1H, 13C, DEPT, COSY, HSQC 및 HMBC 등의 NMR스펙트럼 분석에 의해 밝혀졌다. Daucosterol은 농도 의존적으로 지질분해 효과를 나타내었는데, 본 연구의 결과로부터 뽕나무 잎으로부터 순수분리한 daucosterol의 지분분해 활성은 다양한 질병을 치료하기 위한 천연물 소재 또는 지표성분으로서 활용이 가능할 것으로 생각된다. 그러나 보다 효율적으로 이용하기 위해서는 daucosterol의 비만에 관한 생리활성 기작에 대한 추가적인 연구가 필요할 것이다. Plants are reservoirs of naturally occurring chemical compounds and of structurally diverse bioactive molecules. The aim of this investigation was to screen for the presence of phytochemicals responsible for the lipolysis activity in mulberry (Morus alba) leaves, which are important in traditional Asian medicinal plants. Powdered mulberry leaves were extracted with hexane, ethyl acetate, and methanol. Daucosterol was isolated from the EtOAc extract of mulberry leaves, and its structure was elucidated by NMR spectral analyses. The NMR assignments for the compound were determined using 1H, 13C, DEPT, COSY, HSQC, and HMBC NMR spectral data. Daucosterol showed a concentration-dependent lipolysis activity that may impart medicinal properties that can be exploited by medical practitioners for the treatment of various diseases. However, further studies should be conducted to elucidate additional mechanisms of daucosterol.

딸기 주요성분의 동시분석법 개발 및 국내 재배종과 육성계통의 생리활성 성분 분석

안재욱(Jae Uk An),왕자옥(Ziyu Wang),고민정(Min Jung Ko),이매(Mae Li),강남준(Nam Jun Kang),홍광표(Kwang Pyo Hong),강점순(Jum-Soon Kang),박영훈(Young Hoon Park),김용균(Yong Gyun Kim),최영환(Young Whan Choi) 한국원예학회 2016 한국원예학회 학술발표요지 Vol.2016 No.5

Anti-metastatic Effect of Garlic Hexane Extract on Lung Metastasis Induced by Melanoma B16F10 Cells in Mice

Min Jung Ko(고민정),Seetharaman Rajasekar(라자세커 시타르만),Ziyu Wang(왕자옥),Mei Li(이매),Jung Ho Kwak(곽정호),Young Hoon Park(박영훈),Beung Gu Son(손병구),Jum Soon Kang(강점순),Young Whan Choi(최영환) 한국생명과학회 2016 생명과학회지 Vol.26 No.2

암전이는 현재까지 적당한 치료제가 거의 없었기 때문에 암에 의한 사망의 주요한 원인 중의 하나로 인식되고 있다. 최근 본 연구팀은 마늘 추출물과 순수분리한 성분에 대한 암전이 억제 시험 결과 마늘의 추출물 또는 성분이 암전이를 억제시켰으며, 역학조사에서도 마늘을 많이 섭취한 사람은 암의 발생을 억제시키는 것으로 보고되어 있다, 본 연구의 암전이 실험에서는 C57BL/6 mouse의 꼬리 정맥에 melanoma B16F10세포를 주사하여 폐에 전이를 유도하였다. 암세포 주사 1일 후에 마늘의 헥산 추출물 50, 100 및 200 mg/kg body weight를 2일 간격으로 21일 동안 구강투여 한 다음 암전이 억제효과를 조사하였다. GHE를 처리하지 않은 대조구에서는 폐에서 암 colony가 97.4±30.2으로 대량 생성되었다. GHE를 50, 100 및 200 mg/kg의 농도로 경구투여시에 암전이 빈도는 각각 6.93, 46.80 및 50.53% 억제하였다. 또한 100 mg/kg body weight 경구투여 시에는 폐로 암전이 억제율이 약 53% 이상으로 매우 높았다. 폐에서 melanoma cell colony의 발생율과 면적은 마늘 헥산 추출물의 농도가 높을수록 감소하였다. 결론적으로 C57BL/6 mice의 암전이 모델에서 마늘 헥산추출물의 구강투여는 폐에 암전이를 억제시켰으나, 향후 그 기작에 대한 연구가 수행되어야 할 것으로 생각된다. Metastatic cancer is one of the main causes of cancer-related death since they rarely respond to available treatments. There is epidemiologic evidence that high garlic consumption decreases the incidence of cancer. Recent studies of our laboratory have revealed that a garlic-extracts is effective in suppressing metastasis. For experimental metastasis, C57BL/6 mice were injected intravenously with melanoma B16F10 cells in the tail vein, and were orally administered various concentrations (0, 50, 100 or 200 mg/kg body weight) of garlic hexane extract (GHE) for 21 days. The incidence and the area of the melanoma cell colony occupied by the poorly differentiated carcinoma were significantly lower in dose-dependent in 50, 100 and 200 mg/kg BW GHE–treated mice compared with control mice. In conclusion, the results of the present study show that GHE administration prevents lung metastasis in C57BL/6 mice.