Amycolatopsis sp. KCTC 29142로부터 유래된 siderochelin A의 다제 내성 균주에 대한 항균활성

이동령,성금화,이성권,홍희전,송재경,양승환,서주원,Lee, Dong-Ryung,Cheng, Jinhua,Lee, Sung-Kwon,Hong, Hee-Jeon,Song, Jaekyeong,Yang, Seung Hwan,Suh, Joo-Won The Microbiological Society of Korea 2016 미생물학회지 Vol.52 No.3

본 연구에서는 신규 Amycolatopsis 균주 KCTC 29142를 분리하여 형태학적 관찰, 계통분석 및 화학분류학적 분석 등 다상 분류분석을 통해 분석하였다. KCTC 29142 균주의 에틸아세테이트추출물은 강한 항균활성을 나타났고, 활성물질은 철 이온 킬레이트 물질인 siderochelin A로 동정되었다. 본 연구에서 분리된 siderochelin A는 다제내성균인 Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), 및 Escherichia coli (E. coli)에 대해 강한 활성을 보였고, 임상에서 분리된 다제내성균에 대한 MIC를 결정하였다. A novel Amycolatopsis strain KCTC 29142 was isolated and characterized based on the polyphasic taxonomic analysis including morphological observation, phylogenetic analysis, physiological and chemotaxonomic characteristics. The ethyl acetate extract of strain KCTC 29142 culture broth showed strong antibacterial activity and the active compound was identified as siderochelin A, a ferrous-ion chelating compound. In this study, siderochelin A showed good activity against multi-drug resistant pathogens, including Acinetobacter baumanii, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Staphylococcus aureus (VRSA), and Escherichia coli (E. coli). The minimum inhibitory activity against clinical isolates was also determined.

Discovery of Genomic Regions and Candidate Genes for Awn Length Using QTL-seq in Rice (Oryza sativa L.)

이동령,장홍지아,Yuting Zeng,김백기,권순욱 한국육종학회 2023 Plant Breeding and Biotechnology Vol.11 No.4

Rice domestication has led to cultivated rice with no or short awns. Discovery of novel genes associated to awn lengthis of paramount importance for understanding the molecular mechanisms for the transformation of wild rice long awns to awnlesscultivated rice. In this study, we employed Next-Generation Sequencing based QTL-seq approach to identify genomic regionsassociated with awn length using mapping population derived from a cross between awnless Tun Sart and awned Sobaekmangsudo. QTL-seq analysis identified two awn length QTLs viz. qAwn-4 (12.8-20.3 Mb) and qAwn-8 (22.3-27.2 Mb) on chromosome 4 and 8,respectively. Based on the sequence comparison between the two parents, Os04g0350700 (bHLH transcription factor) was postulatedto be the candidate of Awn-4 gene. Further discovery of the novel genes in qAwn-8 interval will provide insights into the geneticarchitecture of awn length.

isoSTED 현미경의 적응광학 활용을 위한 수차 영향 분석

이동령(D.-R. Lee) Korean Society for Precision Engineering 2023 한국정밀공학회 학술발표대회 논문집 Vol.2023 No.5

디지털 마이크로미러 디바이스를 이용한 고속 3 차원 형상 측정 공초점 현미경

이동령(D.-R. Lee),권대갑(D.-G. Gweon) 한국정밀공학회 2016 한국정밀공학회 학술발표대회 논문집 Vol.2016 No.10월

A PULSED PLASMA SOURCE

Lie, Tong Nyong 서울대학교 1962 서울대학교 論文集 Vol.11 No.-

本論文은 氷表面上에서의 衝擊放電에 關한 硏究結果를 記述하고 있다. 實驗結果에 依하면 放電에 依해서 主로 水表아온 及 電子로 構成된 高密度의 「프리즈마」가 放出되며 2.6×10^6㎝/sec 라는 큰 速力(放電溫度에 關聯되지 않은)으로서 前方으로 推進된다는 것이 밝혀졌다. 더욱이 이 衝擊「프리즈마」는 制限된 空間內에 갇힌채 飛行하며 局部的인 荷電粒子의 密度分布를 갖는 傾向이 있다. 氷表面放電機構의 說明을 爲하여 放電電壓, 電流 및 兩者間의 時間的關聯등이 觀測되었다. 이 結果에 依하면 表面放電(breakdown)은 極히 짧은 時間內(10^-8sec)에 이러나며 이것은 이온化, 氣體放電, arc 放電으로 進行되어 arc 放電終了時에 電極間에 存在하였던 are plasma sample이 前方으로 加速 當한다고 생각된다. 「프리즈마」加速機構는 Button type source의 Bostick 假說과 같이 arc current loop 內側의 보다 强한 磁場의 存在에 基因한다고 推側되나 本「프리즈마」源의 境遇, 그 放電電流가 Button source의 그것의 1/20∼1/100 밖에 되지 않는데 比較的 큰 推進速度를 갖는다는것은 注目할만한 일이다. 本論文은 또 「프리즈마」內의 이온의 數의 推算을 包含한다. A new type of pulsed plasma source which makes use of a pulsed discharge on an ice surface was investigated. The source produces bursts of massive plasma which consists of hydrogen (or deuterium) ions, metallic ions and electrons. It was found that the plasma was projected in forward direction at a speed of 2.6×10^6㎝/sec. in case of 1/3 and ½ microsecond discharge duration and that when the plasma was released from the gap, it was certain density configuration. Mechanism of the discharge was sought and it was inferred that the acceleration of the plasma observed in the present source is possibly caused by the same mechanism as that of the button-type source and in view of the plasma density configuration, it may also be called plasmoid.

Identification of a novel candidate gene for rolled leaf in rice

고희종,이윤경,우미옥,이동령,이길응,김백기 한국유전학회 2016 Genes & Genomics Vol.38 No.11

A semi-narrow and adaxially rolled leaf mutant, rl15(t), was induced from Korean japonica rice cultivar Ilpum by chemical mutagenesis using ethyl methanesulfonate. We characterized the mutant and identified the novel gene causing the mutant phenotype. Cytological analysis of mutant leaves indicated that the adaxial leafrolling phenotype is due to the reduced size and number of bulliform cells in the mutant. Genetic analysis showed that the rolled leaf trait is controlled by a single recessive gene, designated rl15(t). Using an F2 mapping population generated from a cross between Milyang23 and the mutant, we mapped the candidate region to a 174 kb interval on the long arm of chromosome 1 near the centromeric region. Through whole genome sequencing in bulk and MutMap analysis, we identified the causal SNP within the candidate region. The results of RT-PCR analysis indicated that a splicing error occurred due to a base change from G to A at the beginning of the fifth intron of LOC_Os01g37837, which encodes a putative seryl-tRNA synthetase, resulting in the mutant phenotype. Further study of the rl15(t) gene will facilitate analysis of leaf architecture and morphogenesis in rice plants.

Characterization and genetic mapping of white spotted leaf (wspl) mutant in rice

김백기,이혜림,Zhuo Jin,이동령,고희종 한국육종학회 2019 Plant Breeding and Biotechnology Vol.7 No.4

Spotted leaf mutants which produce necrotic lesions spontaneously are important sources to study programmed celldeath in plant defense responses. A novel white-spotted leaf (wspl) mutant was induced from Ilpum, Korean japonica rice cultivar bythe treatment of ethyl methane sulfonate (EMS). The phenotype of wspl mutant differed from that of other spotted leaf mutants in thatnot only brown spots but also white lesion mimic spots were observed on the tip of the leaves from the vegetative stage. Strong NBT andDAB staining were observed on the older leaf of wspl mutant in microscopic ROS assay, and the chlorophyll content of wspl mutantmaintained longer than wild-type in the old leaves. Genetic analysis revealed that the wspl mutant trait was controlled by a singlerecessive gene and the locus of wspl gene was mapped on the long arm of chromosome 5 between the flanking markers S05100 andS05112 (4.1 Mb). Through the combination of the genetic mapping and SNP analysis, two candidate genes for white-spotted leaf wereidentified in the genic region. A novel phenotype of white-spotted leaf mutant has not yet been reported, thus further study of the wsplmutant will contribute to understanding of the molecular mechanisms involved in lesion mimic phenotype in rice.

Immune-enhancing effects of Echinacea purpurea extracts on RAW264.7 cells via TLR4-mediated NF-κB and MAPKs pathways

김혜림,이성권,최봉근,이동령 한의병리학회 2023 동의생리병리학회지 Vol.37 No.5

Echinacea purpurea (L.) Moench is a medicinal plant from North America, Europe, and Australia that has been traditionally used to treat the common cold, bronchitis, coughs, and inflammation of the pharynx and mouth. Furthermore, extracts of Echinacea purpurea (EP) exert various biological effects, such as antioxidant, antibacterial, and anti-inflammatory activities. However, the possible mechanisms of action of the immune-enhancing effects are yet to be elucidated. Therefore, this study investigated the role of EP extracts in the immune-enhancing effects of RAW264.7 cells and the underlying mechanisms of action. It was found that EP extracts considerably increased the protein expression of iNOS, COX-2, and mPGES-1 in RAW264.7 cells. Also, EP extracts increased NO production, phagocytic activity, and the expression of cytokines. Consistent with these results, phosphorylation of MAPKs (ERK, JNK, and p38) and NF-κB (IKKα/β, IκBα, and NF-κB p65) were induced after treatment with EP extracts. Finally, EP extracts caused a marked increase in activation of the TLR4-TRAF6-TAK1 pathway. These results suggest that the immune-enhancing effects of EP extracts are mediated through the TLR4-associated activation of the NF-κB and MAPK pathways in RAW264.7 cells. Thus, it is suggested that EP extracts could be considered as a potential immunostimulatory agent or functional food.

Anti-inflammatory Effects of Scrophularia buergeriana Extract Mixture Fermented with Lactic Acid Bacteria

Thi Ngoc Anh Pham,Hae Lim Kim,이동령,최봉근,양승환 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.3

Scrophularia buergeriana (SB) was once thought to be an herbal remedy with a wide range of uses. Lactobacillus transformation improves the medicinal benefits of biological substances. However, the effect of fermented SBE in macrophages on inflammatory expression is unknown. Furthermore, the synergistic effects of SBE and lactic acid bacteria (LAB) have not been previously investigated. Therefore, in the present study, we investigated the mixture of SB fermented with different lactic acid bacteria (LAB) strains, and analyzed their characteristics before and after fermentation. The results showed that LAB activities increased the levels of phenolic compounds, flavonoids, and iridoids, and SB extract (SBE) stimulated the growth of Lactobacillus strains. Moreover, we also determined the anti-inflammatory effects of the SBE fermented sample in lipopolysaccharide-treated RAW264.7 cells. It was observed that the treatment of SBE fermented sample inhibited nitric oxide (NO) production than SBE and also decreased the RNA expression of iNOS, IL-1β, IL-6, TNF-α, and COX-2. The results of this study indicate the potential anti-inflammation activity of lactic acid bacteria-fermented SBE as nutraceutical ingredients.

Echinacea purpurea extract inhibits LPS-induced inflammatory response by interfering with TLR4-mediated NF-κB and MAPKs signaling pathways

김혜림,민다은,이성권,최봉근,이해진,이동령 한의병리학회 2022 동의생리병리학회지 Vol.36 No.1

Echinacea purpurea (Asteraceae family) is widely used in the European countries and the United States due to its proven immune enhancement and anti-inflammatory effects. Echinacea purpurea has been reported prevent and treat upper respiratory tract infections and common cold, but the underlying molecular mechanisms are not well understood. In the present study, we examined the anti-inflammatory effects and molecular mechanisms of Echinacea purpurea (EP) extract using lipopolysaccharide (LPS)-stimulated signal pathways in RAW264.7 cells. Our results suggest that EP extract exerts anti-inflammatory effects by down-regulating the expression of LPS-induced toll-like receptor 4 (TLR4), subsequently inhibiting the activation of nuclear factor (NF)-κB and mitogen-activated protein kinase (MAPK) signaling pathways and suppression of the release of pro-inflammatory cytokines. These results suggest that EP extract is a potential therapeutic agent for inflammatory diseases.