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The DNAs were extracted and purified from following various vertebrates and invertebrates. Invertebrates: (도표) Vertebrates (도표) These DNA base compositions were analyzed and following results were obtained. Invertebrates Coelenterates and Flatworms The testis DNAs of jelly fish and liver fluke (Fasciola hepatica) have 40% and 42% G + C, respectively. It is noteworthy that DNA base composition of liver fluke is identical to that of its host cells (ox liver) (42 % G + C). Echinoderms The testis DNAs of sea star, sea urchin and sea cucumber have 42 %, 36 % and 39% G + C, respectively. There exists a little variability of G + C content in DNAs among echinoderms Molluscs (1) Loricates The testis DNAs of two species of chitons have the same 37 % G + C. (2) Gastropods The DNA base compositions of gastropod (15 species) testes are variable and generally fall into two classes, one group possessing 37 - 40% G + C and another 30 - 33 % G + C. Testes of limpets (Fissurella) and turban shells (Tegula) have the DNA of 30 - 33 % G + C, and thus these species may belong to pelecypod rather than gastropod in terms of DNA base composition. This exhibits a good example that DNA base composition is not correlated with the traditional classification or taxonomic criteria. (3) Cephalopods The testis DNAs of six species of cephalopods have very similar G + C content (35 - 37 %). (4) Pelecypods Quite similar G + C content (31 - 33%) exists in testis DNAs among fifteen species of pelecypods. There is a significant divergence of G + C contents in DNA among the species of molluscs, but the DNA base proportions show generally a very close similarity in species of the same classes or families except those of gastropods. All the molluscan DNAs are rich in A-T, those of gastropods with exception above mentioned, containing largest amount of G + C. The DNAs of lamellibranchs have the lowest G + C content and those of cephalopods lie in intermediate content an3 those of loricate also rank very high in G + C content. Annelids The earthworm (terrestrial) and sandworm (marine) possess the same 42 % G + C in testis DNA. Arthropod Crustacea: 1) Barnacle: The testes of acorn shell and goose barnacle have the same 43 % G + C in DNA. 2) Crabs: The DNA base compositions of testes of five species of crabs are similar with 36-38% G + C. 3) Lobsters: The testis DNAs of seven species of lobsters are divided into two categories by their G + C contents, one group having 33-34 % G + C contents, and another one about 43 % G + C. This also provided an another illustration that the DNA base proportion was not always consistent with the classical taxonomic criteria, Cambaroides similis, (cray fish) Linuparus trigeners and Panulisus jap. belonging to latter one containing 42-44 % G + C. 4) Stomatopods: The DNA of testis of squilla has 35 % G + C. Insects: The testis DNAs of nine species of insects are of diversity in G + C content which ranges from 33 % to 42 %, but the limited number of our materials cannot be regarded as complete. Arachnids: The testis DNA of a spider possesses 39 % G + C. Chordates The testis of tunicate which lies in transition between invertebrate and vertebrate, has 39 % G + C in DNA. Vertebrates The lowest living vertebrate is hagfish (Cyclostomata), of which testis has the DNA of 42 % G + C, indentical to G + C content of ordinary vertebrates, whereas the testis DNA of various species of sharks have unusual pattern of base composition with relatively high 46 - 49 % G + C, unlike that of other fishes. The DNA bases of testes of varying fishes belonging to teleost, keep the same range of 40 - 43 % G + C. The testis DNA of glove fishes, however, has relatively high G + C content (47 %), and that of ray belonged to elasmobranch has 43 % G + C. The DNA base compositions of testes of amphibia, reptiles, birds and mammals studied all have identical G + C content of 42 - 43%.