낙상홍 추출물의 효능에 관한 연구

이광수,박경숙,Lee, Kwang-Soo,Park, Kyung-Sook 한국식품영양학회 2016 韓國食品營養學會誌 Vol.29 No.6

In this study, Ilex serrata Thunb was extracted with 70% methanol and concentrated under reduced pressure to measure its total polyphenol contents; furthermore, we examined the effect of electron donating ability of the various extracts. The extract was fractionated using different solvents such as methylene chloride, ethyl acetate, and methanol to investigate total polyphenol contents and electron donating abilities. The total polyphenol contents were $1,058.59{\pm}85.85{\mu}g\;GAE/mL$ in 70% methanol, $297.29{\pm}29.43{\mu}g\;GAE/mL$ in methylene chloride, $313.28{\pm}9.22{\mu}g\;GAE/mL$ in ethyl acetate, and $837.82{\pm}31.16{\mu}g\;GAE/mL$ in methanol. The total polyphenol contents showed significant differences (p<0.05) between the solvents. The electron donating abilities were $72.93{\pm}0.29%$ for 70% methanol extract, $14.02{\pm}2.01%$ for methylene chloride, $24.05{\pm}1.75%$ for ethyl acetate, and $84.08{\pm}1.95%$ for methanol. The electron donating abilities were significantly different (p<0.05) between the solvents. The two portions of Ilex serrata Thunb extracts obtained from methanol showed significantly relevant results in the total polyphenol contents and electron donating ability, which was higher than the original extract.

혼식(混植)한 몇가지 수도품종(水稻品種)의 생육(生育)과 병해발생(病害發生)

이광수,안수봉,Lee, Kwang Soo,Ahn, Su Bong 충남대학교 농업과학연구소 1987 Korean Journal of Agricultural Science Vol.14 No.2

Three rice varietis, Dongjinbyeo, Daecheongbyeo and Chucheongbyeo, which are leading cultivars in the Chungnam area, were planted to determine their effects of mixture on the plant characteristics and the blast occurrence. The summary obtained is as follows; 1. The heading dates of each cultivars were not affected by the mixing culture. Only a day was delayed in heading date of Chucheongbyeo under commonly standard fertilizer level. 2. The effects of cultivar mixture on the plant height was high when low level of fertilizer were applied. Of the cultivar mixture, the mixing of Dongjinbyeo and Daecheongbyeo shoed 5.1% of plant height increase. The effects of mixture on the tillering number were apparent only under the standard fertilizer level. The mixture of Dongjinbyeo and Chucheongbyeo was effective in increasing the tillering number up to 5.7% over mean tillering number of each cultivars. 3. The stem height tended to increase as the fertilizer level increased and the effects of cultivar mixture on the stem height was high. The stem height of mixture of Dongjinbyeo and Daecheongbyeo was highest compared with other mixture cropping. On the contrary, the panicle length was shortened as the fertilizer level decreased and cultivars were mixed. Among the cultivar mixtures, the mixture of Dongjinbyeo and Daecheongbyeo showed the most effectives of increasing the panicle length. 4. The total dry weight of mixed and pure cultivars were compared. The mixture of two cultivars was effective in increasing the dry weight of rice upto 3.5% over the average of dry weight of each cultivars. When three cultivars were mixed, there were 8.5% of dry weight increase over not mixed cultivars. The effects of mixture on the dry weight were more apparent under standard fertilizer level. 5. The rates of Neck and Node and Branch Blast occurrence were reduced when cultivars were mixed, and their reduction rates were 11.7% and 14.0%, respectively. The occurrence of Blasts was also decreased under the lowest fertilizer level than the standard fertilizer level. The least Blast occurrence was obtained when Dongjinbyeo and Daecheongbyeo were mixed cropped. 6. Significantly high number of spikelets of per square meter was observed when Chucheongbyeo and Dongjinbyeo were mixed. However, the spikelets of panicle were the lowest when Chucheongbyeo and Dongjinbyeo were mixed. The number of panicles per square meter was increased as the fertilizer levels increased, while the number of spikelets per panicle increased as the fertilizer level decreased. 7. The effects of mixture on the maturing were apparent regardless of the fertilizer level. The 1000 grain weight was higher when lower level of fertilizer was applied. The effects of cultivar mixture on the 1000 grain weight were more apparent in the plots of Chucheongbyeo and Dongjinbyeo mixture and three cultivar mixture. 8. The grain yield increased when cultivars mixed. The increment under mixing cropping was 4.6% over mono cropping. The effects of cultivar mixture on the yield increase were more apparent under lower levels of fertilizer application. The highest yield increase was obtained when Chucheongbyeo and Daecheongbyeo were mixed, and the rate of yield increase was 6.8% over mono-cropping. 9. The grain yield was highly correlated with number of panicles per square meter and dry weight. There was not any significant relationships found among grain yield, spikelets of panicle and ripening percentage. 충남(忠南) 지역(地域) 일반계(一般系) 장려품종인 동진(東津)벼, 대청(大晴)벼, 추청(秋晴)벼 3품종(品種)의 혼식재배(混植栽培)가 병해(病害) 및 수량(收量)에 미치는 영향(影響)을 구명(究明)하기 위하여 1987년(年)에 충남대학(忠南大學) 농과대학(農科大學) 시험포(試驗圃)에서 수행(遂行)한 시험결과(試驗結果)를 요약(要約)하면 다음과 같다. 1. 혼식(混植)에 따른 품종(品種) 및 처리간(處理間) 출수기(出穗期) 변화(變化)는 차이(差異)가 없었고 추청(秋晴)벼 보비구(普肥區)에서만 1일(日) 지연(遲延)되었다. 2. 초장(草長)의 혼식효과(混植效果)는 소비구(少肥區)에서 높았으며 혼식조합별(混植組合別)로는 동진(東津)벼와 대청(大晴)벼 혼식조합구(混植組合區)에서 5.1%로 가장 높게 나타났고 경수(莖數)에서는 보비구(普肥區)에서 혼식효과(混植效果)가 나타났으며 혼식조합별(混植組合別)로는 추청(秋晴)벼와 동진(東津)벼의 혼식조합구(混植組合區)에서 단식구(單植區) 평균치(平均値)보다 5.7%가 증가(增加)하여 혼식효과(混植效果)가 가장 높았다. 3. 간장(稈長)은 시비량(施肥量)이 많을수록 혼식효과(混植效果)도 증가(增加)하였으며 동진(東津)벼와 대청(大晴)벼 혼식조합구(混植組合區)에서 간장(稈長)이 가장 길었으며 혼식효과(混植效果)도 높았다. 반대로 수장(穗長)은 시비량(施肥量)이 많을수록 감소(減少)하였으며 혼식효과(混植效果)도 떨어졌다. 혼식조합별(混植組合別)로는 동진(東津)벼와 대청(大晴)벼 혼식조합구(混植組合區)에서 수장(穗長)이 가장 길었으며, 혼식효과(混植效果)도 현저했다. 4. 성숙기(成熟期)의 지상부(地上部) 건물중(乾物重)은 단식구(單植區)에 비해 2품종(品種) 혼식구(混植區)에서 3.5%, 3품종(品種) 혼식구(混植區)에서 8.5% 증가(增加)하였으며 비료수준별(肥料水準別)로는 보비구(普肥區)에서 혼식효과(混植效果)가 현저했다. 5. 목도열병(稻熱病)과 지경(枝莖) 도열병(稻熱病)은 혼식구(混植區)에서 각각(各各) 11.7%, 14.0% 감소(減少)하였으며, 소비구(少肥區)보다는 보비구(普肥區)에서 혼식효과(混植效果)가 현저했으며, 혼식조합별(混植組合別)로는 동진(東津)벼와 대청(大晴)벼 혼식조합구(混植組合區)에서 도열병(稻熱病) 발생(發生)이 가장 적었다. 6. $m^2$당(當) 수수(穗數)는 추청(秋晴)벼와 동진(東津)벼 혼식조합구(混植組合區)에서 혼식효과(混植效果)가 가장 높았으나 수당(穗當) 영화수(穎花數)는 추청(秋晴)벼와 동진(東津)벼 혼식조합구(混植組合區)에서 가장 낮았다. 비료수준별(肥料水準別) 혼식효과(混植效果)는 $m^2$당(當) 수수(穗數)에서 비료수준(肥料水準)이 높을수록, 수당(穗當) 영화수(穎花數)는 비료수준(肥料水準)이 낮을수록 컸다. 7. 등숙율(登熟率)은 비료수준(肥料水準)에 관계(關係)없이 전(全) 혼식조합구(混植組合區)에서 혼식효과(混植效果)가 나타났으며, 1000립중(粒重)은 소비구(少肥區)에서 혼식조합간(混植組合間)에는 추청(秋晴)벼와 동진(東津)벼 혼식조합(混植組合)과 3품종(品種) 혼식조합구(混植組合區)에서 혼식효과(混植效果)가 높았다. 8. 수량(收量)은 단식구(單植區)보다 혼식구(混植區)에서 4.6% 증수(增收)되었으며 특히 소비구(少肥區)에서 증수(增收)가 현저했다. 혼식조합별(混植組合別)로는 추청(秋晴)벼와 동진(東津)벼 혼식조합구(混植組合區)에서 6.8% 증수(增收)되어 혼식효과(混植效果)가 가장 높았다. 9. 수량(收量)은 $m

지속적인 거주를 위한 노인가구의 주거요구 특성에 관한 연구

이광수,박수빈,Lee, Kwang-Soo,Park, Soo-Been 한국주거학회 2009 한국주거학회 논문집 Vol.20 No.5

As society increasingly ages, maintaining an independent lifestyle at home becomes an important issue for older people. This study aims to determine old people's housing needs for maintaining an independent lifestyle despite their health status and living arrangements. A total of 438 residents voluntarily took part in a research questionnaire survey through the quota sampling method. The participants were grouped according to age (60-64, 65-69, 70-74, and over 75), gender (male and female), and house type (apartment houses and others). The results are as follows. (1) The senior residents are mostly within a non occupational, low income, and low subjective living status. (2) They are satisfied with their current residence and hope to manage the rest of their life in the same place. (3) Three out of five residents prefer the apartment housing type to other types of housing. The preferred dwelling size, number of rooms, and preference for use of an extra room all varied depending on gender and housing type as well as whether they were a couple or living alone. (4) The older residents have a higher need for a safety system than do the younger residents. Female residents pay more attention to convenience while male residents pay more attention to safety. The non-apartment residents require more modification to fundamental facilities such as a heating and ventilation system, wind protection, and additional storage than do the apartment residents. This study has thoroughly analyzed request characteristics according to basic qualities of the elderly households.

The Effect of High Molecular Hyaluronic Acid on Bone Formation in Human Fetal Osteoblasts

이광수,김현아,김윤상,유형근,신형식,Lee, Kwang-Soo,Kim, Hyun-A,Kim, Yun-Sang,You, Hyung-Keun,Shin, Hyung-Shik The Korean Academy of Periodontoloy 2002 Journal of Periodontal & Implant Science Vol.32 No.3

Hyaluronic acid (HA)는 중요한 glycosaminoglycan 중 하나로서 단백질과 화학적 결합을 하지 않기 때문에 분리가 쉽고 결합조직의 세포간 기질의 주요 성분이다. 우리는 점탄성 고분자 hyaluronic acid를 실험실상에서 사람 태아 골모세포의 골 형성 과정에 미치는 영향을 알아보고자 하였다. 우리는 여러 농도의 HA에 대한 사람 태아 골모세포에서의 세포증식, 염기성 인산분해효소 활성, 석회화 결절 형성능, 교원질 합성능 그리고 bone sialoprotein (BSP)의 발현 정도를 검사하였다. 세포증식에서 각 농도의 HA 처리군과 대조군 간에 2일과 4일간의 결과에서 유의한 차이를 보이지 않았다. 염기성 인산분해효소 활성에서는 0.063% HA 처리군에서 음성 대조군에 비해 가장 유의한 염기성 인산분해효소 활성을 보였다 (p<0.05). 0.063% HA 처리군은 교원질 합성능에서도 가장 높은 수준을 보였다 (p<0.05). 석회화 결절 형성능에서는 0.063% HA 처리군에서 대조군에 비해 많은 염색된 석회화 결절을 보였다. BSP의 발현 정도를 분석한 Western blot에서는 대조군에 비해 0.063% HA 처리군에서 증가된 단백질 발현을 나타났다. 본 연구 결과 고분자 HA가 실험실상에서 사람 태아 골모세포의 분화를 통해 새로운 골 형성을 유도할 수 있는 능력이 있음을 시사하였다.

계단식 공동주택 방연풍속 실효성에 대한 연구

이광수,윤명오,이준,Lee, Kwang­Soo,Yoon, Myong­O,Lee, Jun 한국재난정보학회 2021 한국재난정보학회 논문집 Vol.17 No.1

Purpose: The purpose of this study is to analyze the differential pressure and velocity to prevent smoke backflow of Stairways Apartment House fire, and verified the effectiveness of smoke velocity standards proposed by NFSC 501A. Method: The smoke control design of the stairways apartment house of the real model and the performance of the velocity to prevent smoke backflow according to the window opening conditions of the living room were analyzed using the CONTAM program. Result: Although the differential pressure performance of the apartment's smoke control system was satisfactory, it was found that Performance of velocity to prevent smoke backflow did not come out according to the opening condition of the living room window. Conclusion: In the case of Stairways Apartment House, it is necessary to review the method of making exceptions to the 'velocity to prevent smoke backflow' standard required by the National Fire Safety Codes(NFSC 501A) 연구목적: 본 연구는 계단식 공동주택의 화재 시 차압 및 방연풍속 특성을 분석하고, 화재안전기준에서 제시하고 있는 방연풍속 기준의 실효성을 검증하는 것을 목적으로 한다. 연구방법: 실제 모델의 계단식 공동주택의 제연설계 및 거실의 창문 개방조건에 따른 방연풍속의 성능을 CONTAM 프로그램을 이용하여 분석하였다. 연구결과: 공동주택 제연설비의 차압성능은 만족하더라도, 방연풍속 성능은 거실창문의 개방조건에 따라 방연풍속의 성능이 나오지 않음을 알 수 있었다. 결론: 계단식 공동주택의 경우 화재안전기준에서 요구하는 '방연풍속' 기준에 대해 예외를 두는 방안의 검토가 필요하다.

나문재(Suaeda glauca (Bunge) Bunge) 추출물과 분획물의 효능에 관한 연구

이광수,박경숙,Lee, Kwang-Soo,Park, Kyung-Sook 한국식품영양학회 2019 韓國食品營養學會誌 Vol.32 No.6

In this study, S. glauca (Bunge) Bunge extract was testified total polyphenol contents, electron donating ability on DPPH, and scavenging ability of superoxide anion radical and hydrogen peroxide. Total polyphenol contents of S. glauca (Bunge) Bunge extract were 7,053.30±915.93 ㎍ GAE/mL. In the fractions, fraction by ethanol was the highest content of 10,973.30±1,000.24 ㎍ GAE/mL. Fractions of ethyl acetate and water were 2,386.70±166.53 ㎍ GAE/mL, 2,413.30±41.63 ㎍ GAE/mL respectively. It was shown that total content of polyphenol according to solvent was significant relation at p<0.05. In the experiment of the electron donating ability, 70% ethanol extract and methylene chloride fraction were -246.15±24.17%, -254.01± 16.54% respectively. In case of ethyl acetate fraction, it was the highest electron donating ability to DPPH radical, 39.06±0.34%, and then water fraction of 36.71±5.55%, ethanol fraction of 29.77±2.57%, gradually. Electron donating abilities revealed significant difference (p<0.05) between the solvents. The superoxide radical scavenging ability of standard material was 0.029±0.0011; 70% ethanol extract of 0.022±0.00052, methylene chloride fraction of 0.027±0.00031, ethyl acetate fraction of 0.024±0.0011, ethanol fraction of 0.021±0.00024 and water fraction of 0.024±0.00019. Hydrogen peroxide scavenging ability of 70% ethanol extract was -0.0029±0.00040 and the others were as follow; methylene chloride fraction (-0.0042±0.00058), ethyl acetate fraction (-0.003± 0.0041), ethanol fraction (-0.0029±0.0015) and water fraction (-0.0028±0.00090).

커피 폐기물 추출물의 효능에 관한 연구

이광수,박경숙,Lee, Kwang-Soo,Park, Kyung-Sook 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.5

In this study, coffee waste was extracted with different solvents such as ethyl acetate, methylene chloride and methanol to investigate the total polyphenol contents, electron donating ability and the inhibitory effect on glutathione S-transferase. The total polyphenol contents were $3,060.61{\pm}357.12{\mu}g\;GAE/mL$ in ethyl acetate, $909.09{\pm}35.71{\mu}g\;GAE/mL$ in methylene chloride, and $1,602.27{\pm}30.36{\mu}g\;GAE/mL$ in methanol. The total polyphenol contents showed a significant difference (p<0.05) between the solvents. The electron donating ability was $80.20{\pm}1.45%$ for ethyl acetate, $81.94{\pm}0.45%$ for methylene chloride, and $85.14{\pm}1.53%$ for methanol. The electron donating abilities were significantly different (p<0.05) between the solvents. The inhibitory effect of the various extracts on glutathione S-transferase (% inhibition) was $92.12{\pm}0.56%$, $88.48{\pm}0245%$ with methylene chloride extract, and $90.85{\pm}0.14%$ with methanol extract. These too were significant different (p<0.05) between the solvents. The two portions of coffee waste extracts obtained from ethyl acetate and methanol showed meaningful results on the total polyphenol contents, and the inhibition effects on glutathione S-transferase. Therefore, they can be utilized to develop health care foods and can be applied as antioxidants for cosmeceuticals.

양파 추출물에서의 글루타티온 전달효소 활성 저해제에 관한 연구

이광수,박경숙,Lee, Kwang-Soo,Park, Kyung-Sook 한국식품영양학회 2013 韓國食品營養學會誌 Vol.26 No.4

In this study, an ethanol extract being obtained from Allium cepa var. cepa examins the inhibitory effects on the glutathione S-transferase and the separation had been done by silica-gel column chromatography using various eluents, such as ethyl acetate, methanol, and 50% methanol. A volume of column fraction was 50ml and evaporation has been performde by the rotary evaporator under reduced pressure. Each fraction is being examined by thin layer chromatography and the UV spectrum at 365 nm was used to investigate separation patterns of spots on thin layer chromatography. When the eluent was changed, the spot patterns showed another different pattern on thin layer chromatography, so on. Fractions showing similar pattern are combined and eventually, three fractions are obtained. Each fraction is testified to examine the inhibition effects on glutathione S-transferase. All of these showed inhibition effects on glutathione S-transferase. The GC-MS shows that each fraction contains more than 2 compounds.

관상동맥우회로술 환자의 위험도에 따른 수술량과 병원내 사망의 관련성

이광수,이상일,Lee, Kwang-Soo,Lee, Sang-Il 대한예방의학회 2006 예방의학회지 Vol.39 No.1

Objectives: To propose a risk-adjustment model with using insurance claims data and to analyze whether or not the outcomes of non-emergent and isolated coronary artery bypass graft surgery (CABG) differed between the low- and high-volume hospitals for the patients who are at different levels of surgical risk. Methods: This is a cross-sectional study that used the 2002 data of the national health insurance claims. The study data set included the patient level data as well as all the ICD-10 diagnosis and procedure codes that were recorded in the claims. The patient's biological, admission and comorbidity information were used in the risk-adjustment model. The risk factors were adjusted with the logistic regression model. The subjects were classified into five groups based on the predicted surgical risk: minimal (<0.5%), low (0.5% to 2%), moderate (2% to 5%), high (5% to 20%), and severe (=20%). The differences between the low- and high-volume hospitals were assessed in each of the five risk groups. Results: The final risk-adjustment model consisted of ten risk factors and these factors were found to have statistically significant effects on patient mortality. The C-statistic (0.83) and Hosmer-Lemeshow test ($x^2=6.92$, p=0.55) showed that the model's performance was good. A total of 30 low-volume hospitals (971 patients) and 4 high-volume hospitals (1,087 patients) were identified. Significant differences for the in-hospital mortality were found between the low- and high-volume hospitals for the high (21.6% vs. 7.2%, p=0.00) and severe (44.4% vs. 11.8%, p=0.00) risk patient groups. Conclusions: Good model performance showed that insurance claims data can be used for comparing hospital mortality after adjusting for the patients' risk. Negative correlation was existed between surgery volume and in-hospital mortality. However, only patients in high and severe risk groups had such a relationship.

6,6-Dichlorobicyclo[3, 1, 0]hexane-3-carboxylic acid의 합성과 분석

이광수,양재건,Lee, Kwang-Soo,Yang, Jae-Kun 한국분석과학회 2001 분석과학 Vol.14 No.1

6,6-Dichlorobicyclo[3, 1, 0]hexane-3carboxylic acid was synthesized by dichlorocarbene addition into 3-cyclopentenecarboxylic acid using BTEA.Cl as phase transfer catalyst. $^1H$ NMR $^{13}C$ NMR data analyst showed that this compound had boat-like conformation and carboxyl group existed as trans form. 6,6-Dichlorobicyclo[3, 1, 0]hexane-3carboxylic acid를 phase transfer catalyst(PTC)로 benzyl triethylamine chloride(BTEA.Cl)를 사용하여 3-cyclopentenecarboxylic acid로부터 합성하였다. $^1H$ NMR과 $^{13}C$ NMR 분석을 통하여 이 화합물은 boat-like conformation을 갖는 것으로 나타났고 carboxyl group은 trans로 존재하는 것으로 나타났다.