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頭部에서 Pityrosporum orbiculare 및 Pityrosporum ovale의 硏究
이건섭,방용준 대구보건대학 1995 대구보건대학 論文集 Vol.15 No.-
The authors performed mycologic studies on Pityrosporum by direct smear culture. 1. The subjects with dandruff were 63.3% and subjects without dandruff were 36.7%. 2. The spores of Pityrosporum were observed in all subjects under the KOH microscopic examination. 3. The subjects in the group of more than 90% of P.orbiculare were 42, group of 90-60% of P.orbiculare were 2, group of more than 90% of P.oval were 34, group of 90-60% of P.ovale were 12. 4. Scales from the scalp of 60 subjects were cultured, positive culture colonies were observed in 60(100%).
이건섭,서민호,백성덕 대구보건대학 1994 대구보건대학 論文集 Vol.14 No.-
The development of recombinant DNA technology bring about a revolution in biology. In medicine molecular biology is widely appolicated to diagnosis, treatment and understanding of disease. For diagnosis of infectious disease, cancer and genetic disorder, recombinant DNA technology provides rapid, specific, sensitive new method ; Southern blot, Northern blot, in situ hybridization and PCR(Polymerase Chain Reaction), etc. PCR is being used for rapid and sensitive diagnostic method of infectious disease, for example for diagnosis fo Mycobacterium tuberculosis classic culture method needs 4~7 weeks but PCR only 1~2 days. Over the nest few years by combing automated method for DNA extraction, PCR, and non-radioactive labelling, it may be possible to automate most of steps in molecular diagnosis of disease.
이건섭,조승찬,Phuong Mai Hoang,김동준,이용준,길의준,변성준,김택균,김대현,김성한,이석찬 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.9
3D8 single chain variable fragment (scFv) is a recombinant monoclonal antibody with nuclease activity that was originally isolated from autoimmune-prone MRL mice. In a previous study, we analyzed the nuclease activity of 3D8 scFv and determined that a HeLa cell line expressing 3D8 scFv conferred resistance to herpes simplex virus type 1 (HSV- 1) and pseudorabies virus (PRV). In this study, we demonstrate that 3D8 scFv could be delivered to target tissues and cells where it exerted a therapeutic effect against PRV. PRV was inoculated via intramuscular injection, and 3D8 scFv was injected intraperitoneally. The observed therapeutic effect of 3D8 scFv against PRV was also supported by results from quantitative reverse transcription polymerase chain reaction, southern hybridization, and immunohistochemical assays. Intraperitoneal injection of 5 and 10 μg 3D8 scFv resulted in no detectable toxicity. The survival rate in C57BL/6 mice was 9% after intramuscular injection of 10 LD50 PRV. In contrast, the 3D8 scFv-injected C57BL/6 mice showed survival rates of 57% (5 μg) and 47% (10 μg). The results indicate that 3D8 scFv could be utilized as an effective antiviral agent in several animal models.