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이강선(Gang Seon Lee),남기열(Ki Yeul Nam),최재을(Jae Eul Choi) 한국약용작물학회 2013 한국약용작물학회지 Vol.21 No.4
This study was conducted to compare the contents of ginsenoside according the water extract conditions of red ginseng. In method A, red ginseng extract was prepared at 75℃ for 18 hours by 1 time extraction, and method B, the preparation was done at 85℃ for 18 hours by 1 time extraction. In method C, the primary extract prepared at 75℃ for 9 hours was blended with the secondary extract prepared by re-extracting the red ginseng residue obtained after the primary extraction, at 85℃ for 9 hours. Method D was the same procedure as method C but the extraction temperature for the primary extraction was 85℃ and that for the secondary extraction was 95℃. The contents of total and Rb1, Rg1 and Rg₃ ginsenoside were highest in Method C. The content of prosapogenin (ginsenoside Rg2, Rg₃, Rh1 and Rh2) was highest in Method B. There was no consistent tendency in Brix, pH, Hue value and absorbance among extraction methods.
우이음 ( E-eum Woo ),이강선 ( Gang-seon Lee ),이인경 ( In-kyoung Lee ),최재을 ( Jae-eul Choi ),윤봉식 ( Bong-sik Yun ) 한국균학회 2016 韓國菌學會誌 Vol.44 No.3
한국의 인삼(Panax ginseng)은 다양한 생리학적, 약리학적 활성을 지닌 것으로 입증되어 왔다. 인삼 잘록병은 Rhizoctonia solani와 Pythium sp.과 같은 식물 병원균에 의해 발생하며 인삼 종자에 치명적이다. 잘록병은 일반적으로 화학농약을 이용하여 방제하고 있다. 또한 생물학적 방제는 인삼 잘록병을 방제하는데 효과적이며 환경친화적인 방법으로 알려져 있다. 본 연구진은 인삼 잘록병 방제를 위한 생물 방제제로서 잠재성을 지닌 토양 세균을 탐색하였으며, 그 결과 항균물질 guanidylfungin과 methylguanidylfungin을 생산하는 방선균 A3265 균주를 선발하였다. 본 연구는 방선균 A3265 균주의 인삼 잘록병 방제 활성을 포장에서 조사한 것이다. 그 결과 인삼종자를 방선균 A3265균주의 배양액에 침지한 후 파종하였을 때 잘록병의 발병률이 유의적으로 감소함을 확인하였다. 이는 방선균 A3265균주가 잘록병 방제에 효과적으로 활용될 수 있음을 나타낸다. Korean ginseng (Panax ginseng) possesses various biological and pharmacological properties. Damping-off is a critical disease on ginseng seedlings, which is caused by the fungal pathogens Rhizoctonia solani and Pythium sp.. This disease is generally controlled by the application of fungicides, but also biological control is an efficient and environmentally friendly way to prevent ginseng damping-off. In a previous study, we screened soil-borne bacteria with potential applications as biological control agents for ginseng damping-off and selected the bacterial strain Streptomyces sp. A3265, producing antifungal substances guanidylfungin and methylguanidylfungin. In this study, we investigated control efficacy of Streptomyces sp. A3265 against ginseng damping-off in the field. As a result, the incidence of damping-off was significantly reduced when soaking ginseng seeds in the culture broth of Streptomyces sp. A3265.