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한우에서 Computer aided semen analysis(CASA) 기법을 이용한 수태율 예측에 관한 연구
이강남,이병천,황우석,Lee, Kang-nam,Lee, Byeong-chun,Hwang, Woo-suk 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4
This experiment was conducted to predict the effects of motional characteristics on the fertility of Korean native cattle(KNC) by using CASA technology and in vitro fertilization system. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system(SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber(Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The oocytes collection, in vitro maturation, IVF, in vitro culture and determination of the cleavage rate were performed by the technique, as described by Hwang et al (1997). Statistical analysis was done by linear regression with use of the Sigma plot program on a IBM personal computer. The cleavage rate in vitro fertilized oocyte was significantly correlated(P<0.05) with MOT, VCL, VSL, VAP, ALH, BCF and MAD, but not CON, LIN, STR, WOB, DNM, DNC and HYP in regressional analysis. The results show that some kinematic characteristics of frozen-thawed semen by CASA can be predict the fertility in in vitro model system.
컴퓨터 정액자동분석에 의한 동결융해 한우 정액의 운동특성 연구
이강남,이병천,김정태,박종임,신태영,황우석,Lee, Kang-nam,Lee, Byeong-chun,Kim, Jung-tae,Park, Jong-im,Shin, Tae-young,Hwang, Woo-suk 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.4
The aim of this experiments were to assess the time-interval change of motional characteristics in frozen-thawed semen of Korean native cattle (KNC) by using computer aided semen analysis (CASA) technology. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system (SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber (Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The measurement parameters in SIAS were as follows ; frame rate = 30 frames per sec, image capture = 1 sec, minimum motile speed = $10{\mu}m/s$, maximum countable sperm number = 400. Statistical analysis was done by Student t-test with use of the Sigma plot program on a IBM personal computer. The dancemean(DNM) and hyperactivated sperm(HYP) of frozen-thawed KNC semen kinematics were significantly decreased(p < 0.05) after 10 min of incubation at $37^{\circ}C$ water bath. But, wobble(WOB) of same sample semen was significantly increased(p < 0.05) after 10 min of incubation and significantly decrease(p < 0.05) after 60 min of same incubation. And, after 30 mim of incubation, significantly differences were found most of motion kinematics, motifity(MOT), curvilinear velocity(VCL), straight line velocity(VSL), average path velocity(VAP), amplitude of lateral head displacement(ALH), beat cross frequency(BCF), mean angular displacement(MAD), dance(DNC), on same sample semen. The DNM of KNC semen sample was variable kinematics after 30 min of incubation. Also, the linearity(LIN) and straightness(STR) was significantly decreased(p < 0.05) from 60 min of incubation. In conclusion, the AI within 30 min after thawing of frozen semen can be an effective method for obtaining high fertility rate in KNC reproductive program.
치은증식시 세포구성과 성장인자에 관한 면역조직화학적 연구
이강남,한수부,이재일,Lee, Kang-Nam,Han, Soo-Boo,Lee, Jae-Il 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.2
The purpose of this study was to investigate the differences of histochemical characteristics in inflammatory fibrous gingival hyperplasia (FGH), phenytoin-induced gingival hyperplasia(PIGH), idiopathic gingival hyperplasia(IDGH) and control groups (healthy and inflammatory gingiva) by immunohistochemical method with various antibodies and histomorphological analysis. In immunohistochemical finding, antibodies to inflammatory cells (T/B lymphocytes, macrophages, other monocytes), proliferating cell nuclear antigen(PCNA), epidermal growth factor(EGF), factor VIII, and type I collagen were used. 1. The inflammatory infiltrates in FGH were less than those in inflammatory gingiva. The composition of inflammatory cells of PIGH was similar with that of FGH. IDGH showed a similar histologic findings with healthy gingival tissue. 2. In FGH, the number of fibroblasts and newly-formed collagen fibers was increased. No significant increase of fibroblasts and the dense accumulation of thick collagen fibers were seen in PIGH. The increase of fibroblasts and the dense accumulation of thick collagen were seen in IDGH. 3. PCNA-positive cells were localized mainly in the area accumulated with inflammatory cells and blood vessels, significantly increased in all hyperplastic tissue groups, and distributed evenly in IDGH. 4. The distribution of EGF were not observed in healthy gingiva but detected locally in area with confluent blood vessels,without significant difference between the other tissue groups. This results suggest that inflammation plays a significant role in inducing hyperplastic change of gingival tissue. While in DIGH, drug itself as well as inflammation seems to attribute to hyperplastic change.
플레어 각도에 따른 청수현상의 발생과정 및 갑판 위 유동특성 변화에 대한 실험적 연구
이강남(Gang Nam Lee),정광효(Kwang Hyo Jung),서성부(Sung Bu Suh),김문성(Mun Sung Kim) 한국해양공학회 2018 韓國海洋工學會誌 Vol.32 No.2
In this study, a series of experiments were performed to investigate the variations in the behavior of green water generation and the flow kinematics of bubbly flow on deck with various flare angles. The experiments were conducted in a 2-D wave flume using a simplified model of a BW Pioneer FPSO operating in the Gulf of Mexico, with a 100-year return period wave condition. The green water phenomena were captured with a high speed CCD camera. The variations in the behavior of the green water generation were investigated with various flare angles, and the horizontal mean velocity profiles of bubbly flow on deck obtained using bubble image velocimetry (BIV) were provided. The differences in flow kinematics of bubbly flow on deck were analyzed with various flare angles.
이강남(K. N. Lee),정광효(K. H. Jung),채영준(Y. J. Chae),박일룡(I. R. Park),서성부(S. B. Suh) 한국전산유체공학회 2015 한국전산유체공학회지 Vol.20 No.2
In this study, the green water phenomena on rectangular shaped structure is numerically simulated by STAR-CCM+ to investigate the flow pattern including the velocity profiles in bubbly water flow. 5 phases of the formation of green water in front of and over the rectangular shaped structure is simulated at the design condition which is scaled down by 1:125 from FPSO operating in GOM. All numerical results are compared with the experimental results performed in a two dimensional wave flume. The water deformation due to the green water are obtained by the high speed CCD camera with employing the shadow graphy technique, which is allowed to take the bubbly water flow into images. A series of image taken by shadow graphy technique is analyzed with MQD method to calculate the velocity in bubbly water flow.