http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Efficiency of an Automated Reception and Turnaround Time Management System for the Phlebotomy Room
윤순규,신정원,박은수,방해인,강중구 대한진단검사의학회 2016 Annals of Laboratory Medicine Vol.36 No.1
Background: Recent advances in laboratory information systems have largely been focused on automation. However, the phlebotomy services have not been completely automated. To address this issue, we introduced an automated reception and turnaround time (TAT) management system, for the first time in Korea, whereby the patient’s information is transmitted directly to the actual phlebotomy site and the TAT for each phlebotomy step can be monitored at a glance. Methods: The GNT5 system (Energium Co., Ltd., Korea) was installed in June 2013. The automated reception and TAT management system has been in operation since February 2014. Integration of the automated reception machine with the GNT5 allowed for direct transmission of laboratory order information to the GNT5 without involving any manual reception step. We used the mean TAT from reception to actual phlebotomy as the parameter for evaluating the efficiency of our system. Results: Mean TAT decreased from 5:45 min to 2:42 min after operationalization of the system. The mean number of patients in queue decreased from 2.9 to 1.0. Further, the number of cases taking more than five minutes from reception to phlebotomy, defined as the defect rate, decreased from 20.1% to 9.7%. Conclusions: The use of automated reception and TAT management system was associated with a decrease of overall TAT and an improved workflow at the phlebotomy room.
Rhodocyclus gelatinosus KUP-74의 고정화균체로부터 ${\delta}-Aminolevulinate$의 연속생산
이소희,김현호,윤순규,임왕진,황세영,Lee, So-Hee,Kim, Hyun-Ho,Yun, Sun-Kyu,Lim, Wang-Jin,Hwang, Se-Young 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
Rhodocyclus gelatinosus KUP-74의 고농도 휴지균체로부터 ${\delta}-aminolevulinate$(ALA)를 연속적으로 생산하기 위한 최적조건을 검토하였다. 균체외 ALA양은 20 mg cells/ml의 균체농도까지는 농도 증가에 따른 rectangular hyperbola의 증가양식을 보였으나, 그 이상의 균체농도는 ALA 생산에 전혀 증가효과를 보이지 않았다. 20 mg cells/ml의 반응계에서, 균체외 ALA의 효과적 생산을 위한 첨가물질의 최저농도는 levulinate 4 mM과 L-glutamate 5 mM로 나타났으며, 배양 3시간후 ALA 양의 최대치를 보였다. 한편, Ca-alginate를 이용한 포괄법으로 고정화시킨 균체로부터 효과적으로 ALA를 생산하기 위한 첨가물질의 최적농도는 levulinate 6 mM과 L-glutamate 10 mM로 나타났으며, 배양 6시간째에 균체외 ALA 양의 최대치를 보였다. 또한, 이 조건 하에서 고정화균체와 고농도의 휴지균체에 의한 ALA의 연속생산성을 검토한 결과, 이들 균체에 의한 50% ALA 생산성은 각각 185시간과 100시간에서 나타났으며, 생산된 균체외 ALA의 회수를 위해서는 균체를 고정화시키는 방법이 효과적이었다. An optimal condition for the continuous production of ${\delta}-aminolevulinate$(ALA) was investigated using high concentrated resting cells of Rhodocyclus gelatinosus KUP-74. The increase of the amount of extracellular ALA versus the concentration of resting cells showed rectangular hyperbolic pattern until 20 mg cells/ml, but no further increase in the ALA amount by increasing its concentration was occurred. The highest yield of the extracellular ALA was observed after 3 hr of incubation of 1 ml reaction system containing 20 mg cells, 4 mM levulinate and 5 mM L-glutamate. On the other hand, the immobilized cells prepared by Ca-alginate inclusive method needed to incubate for 6 hr with 6 mM levulinate and 10 mM L-glutamate to give maximal yield of the extracellular ALA. In addition, under these conditions the resulted continuous productivities of the ALA by immobilized cells and highly concentrated resting cells were appeared 50 percent decreases after incubations for 185 hr and 100 hr, respectively, and the method of the cells to be immobilized was more efficient to recover the extracellular ALA produced.
폐색전증 진단을 위한 D-dimer 검사의 유용성: 단일기관연구
박노진,서영익,윤순규,최태윤,신정원,어수택,김양기 대한진단검사의학회 2008 Annals of Laboratory Medicine Vol.28 No.6
Background : Pulmonary embolism (PE) presents with diverse non-specific signs and symptoms and its diagnosis mainly depends on diagnostic imaging tests which are laborious and not costeffective, and only a small proportion of patients with suspected PE actually have the disease. The aim of this study was to analyze the utility of D-dimer test for diagnosing PE by categorizing patients into ‘PE likely’ and ‘PE unlikely’ groups using Wells score for clinical probability. Methods : One hundred forty consecutive patients with clinically suspected PE, in whom D-dimer and imaging tests were performed were enrolled. Dignosis of PE was made when the imaging tests were positive. Wells scores were retrospectively assigned and the dignostic utility of D-dimer test was analyzed. Results : Of the 140 patients studied, D-dimer test was positive in 97 and diagnostic imaging tests revealed PE, deep vein thrombosis (DVT), and PE+DVT in 24, 3, and 7 patients, respectively. For the diagnosis of PE, D-dimer test with cutoff value of ≥230 ng/mL showed sensitivity, specificity, and negative predictive value of 96.8%, 39.6%, and 97.7%, respectively. These values were 96.3%, 37.9%, and 91.7% in ‘PE likely’ group (n=56), and 100%, 38.8%, and 100% in ‘PE unlikely’ group (n=84). Among 43 patients with D-dimer values of <230 ng/mL, only one patient was diagnosed with PE, who belonged to the ‘PE likely’ group. Conclusions : D-dimer test cannot be used as a stand-alone test to diagnose PE, but it can be helpful for exclusion of PE especially in ‘PE unlikely’ group according to Wells score. (Korean J Lab Med 2008;28:419-24)
박은수,조경일,신정원,박노진,최태윤,방해인,채금란,윤순규 대한진단검사의학회 2014 Annals of Laboratory Medicine Vol.34 No.3
Background: Most immune reactions related to transfusion and transplantation are caused by IgM ABO antibodies. However, IgG also plays an important role in these reac- tions. Therefore, a method to measure antibodies, including IgG, is necessary. We investi- gated ABO antibody titers of healthy individuals using a column agglutination technique (CAT) with or without dithiothreitol (DTT) and compared them with titers obtained using a conventional tube method. Methods: Among healthy adults who underwent a medical examination, 180 individuals (60 with blood group A, 60 with group B, and 60 with group O) were selected. Antibody ti- trations were performed using the immediate spin (IS) tube, anti-human globulin (AHG) tube, and CAT with or without DTT methods. Results: Higher median values of anti-B and anti-A titers in groups A and B individuals, respectively, were obtained using the IS method than using the AHG method. Higher val- ues for group O individuals were obtained using the AHG method. Higher median titers of anti-B and anti-A in group O individuals were obtained using CAT without DTT than using the AHG method. Median titers of anti-B and anti-A in all blood groups were higher in CAT without DTT than in CAT with DTT, especially for group O individuals. Conclusions: We recommend CAT with and without DTT for titration of anti-A and anti-B, especially in group O individuals, to provide more sensitive results that include IgG data. Adjustment of insurance coverage of fees associated with antibody titration might be nec- essary, considering the actual cost of reagents and personnel.
Rhodocyclus gelatinosus KUP - 74 의 고정화균체로부터 δ - Aminolevulinate 의 연속생산
이소희(So He Lee),김현호(Hyun Ho Kim),윤순규(Sun Kyu Yun),임왕진(Wang Jin Lim),황세영(Se Young Hwang) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
An optimal condition for the continuous production of δ-aminolevulinate(ALA) was investigated using high concentrated resting cells of Rhodocyclus gelatinosus KUP-74. The increase of the amount of extracellular ALA versus the concentration of resting cells showed rectangular hyperbolic pattern until 20 ㎎ cells/㎖, but no further increase in the ALA amount by increasing its concentration was occurred. The highest yield of the extracellular ALA was observed after 3 hr of incubation of 1 ㎖ reaction system containing 20 ㎎ cells, 4 mM lewlinate and 5 mM L-glutamate. On the other hand, the immobilized cells prepared by Ca-alginate inclusive method needed to incubate for 6 hr with 6 mM levulinate and 10 mM L-glutamate to give maximal yield of the extracellular ALA. In addition, under these conditions the resulted continuous productivities of the ALA by immobilized cells and highly concentrated resting cells were appeared 50 percent decreases after incubations for 185 hr and 100 hr, respectively, and the method of the cells to be immobilized was more efficient to recover the extracellular ALA produced.